UniProtKB - Q66H62 (CYLD_RAT)
Ubiquitin carboxyl-terminal hydrolase CYLD
Cyld
Functioni
Catalytic activityi
- Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).By similarity EC:3.4.19.12
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 598 | NucleophilePROSITE-ProRule annotation | 1 | |
Metal bindingi | 785 | Zinc 1By similarity | 1 | |
Metal bindingi | 788 | Zinc 1By similarity | 1 | |
Metal bindingi | 796 | Zinc 2By similarity | 1 | |
Metal bindingi | 799 | Zinc 2By similarity | 1 | |
Metal bindingi | 814 | Zinc 1By similarity | 1 | |
Metal bindingi | 817 | Zinc 1By similarity | 1 | |
Metal bindingi | 822 | Zinc 2By similarity | 1 | |
Metal bindingi | 830 | Zinc 2By similarity | 1 | |
Active sitei | 868 | Proton acceptorPROSITE-ProRule annotation | 1 |
GO - Molecular functioni
- cysteine-type peptidase activity Source: UniProtKB-KW
- Lys48-specific deubiquitinase activity Source: RGD
- Lys63-specific deubiquitinase activity Source: UniProtKB
- proline-rich region binding Source: RGD
- protein kinase binding Source: RGD
- thiol-dependent ubiquitin-specific protease activity Source: UniProtKB
- zinc ion binding Source: UniProtKB
GO - Biological processi
- CD4-positive or CD8-positive, alpha-beta T cell lineage commitment Source: RGD
- homeostasis of number of cells Source: RGD
- innate immune response Source: UniProtKB
- necroptotic process Source: RGD
- negative regulation of canonical Wnt signaling pathway Source: UniProtKB
- negative regulation of JNK cascade Source: UniProtKB
- negative regulation of NF-kappaB transcription factor activity Source: UniProtKB
- negative regulation of NIK/NF-kappaB signaling Source: UniProtKB
- negative regulation of p38MAPK cascade Source: UniProtKB
- negative regulation of T cell differentiation Source: RGD
- positive regulation of cellular protein localization Source: RGD
- positive regulation of extrinsic apoptotic signaling pathway Source: RGD
- positive regulation of T cell differentiation Source: RGD
- positive regulation of T cell receptor signaling pathway Source: RGD
- protein deubiquitination Source: UniProtKB
- protein K63-linked deubiquitination Source: UniProtKB
- protein linear deubiquitination Source: UniProtKB
- regulation of B cell differentiation Source: RGD
- regulation of cilium assembly Source: UniProtKB
- regulation of inflammatory response Source: UniProtKB
- regulation of intrinsic apoptotic signaling pathway Source: RGD
- regulation of microtubule cytoskeleton organization Source: RGD
- regulation of mitotic cell cycle Source: RGD
- regulation of necroptotic process Source: RGD
- regulation of protein binding Source: RGD
- regulation of tumor necrosis factor-mediated signaling pathway Source: UniProtKB
- ripoptosome assembly involved in necroptotic process Source: RGD
- ubiquitin-dependent protein catabolic process Source: InterPro
- Wnt signaling pathway Source: UniProtKB-KW
Keywordsi
Molecular function | Hydrolase, Protease, Thiol protease |
Biological process | Immunity, Innate immunity, Ubl conjugation pathway, Wnt signaling pathway |
Ligand | Metal-binding, Zinc |
Enzyme and pathway databases
Reactomei | R-RNO-168638, NOD1/2 Signaling Pathway R-RNO-5357786, TNFR1-induced proapoptotic signaling R-RNO-5357905, Regulation of TNFR1 signaling R-RNO-5357956, TNFR1-induced NFkappaB signaling pathway R-RNO-5689880, Ub-specific processing proteases |
Protein family/group databases
MEROPSi | C67.001 |
Names & Taxonomyi
Protein namesi | Recommended name: Ubiquitin carboxyl-terminal hydrolase CYLD (EC:3.4.19.12By similarity)Alternative name(s): Deubiquitinating enzyme CYLD Ubiquitin thioesterase CYLD Ubiquitin-specific-processing protease CYLD |
Gene namesi | Name:Cyld Synonyms:Cyld1 |
Organismi | Rattus norvegicus (Rat) |
Taxonomic identifieri | 10116 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Rattus |
Proteomesi |
|
Organism-specific databases
RGDi | 1308346, Cyld |
Subcellular locationi
Cytoskeleton
- cytoskeleton
- centrosome By similarity
- spindle By similarity
- cilium basal body By similarity
Cytoplasm and Cytosol
Plasma membrane
- Cell membrane By similarity; Peripheral membrane protein By similarity; Cytoplasmic side By similarity
Other locations
Note: Detected at the microtubule cytoskeleton during interphase (By similarity). Detected at the midbody during telophase (By similarity). During metaphase, it remains localized to the centrosome but is also present along the spindle (By similarity).By similarity
Cytoskeleton
- centrosome Source: UniProtKB
- ciliary basal body Source: UniProtKB
- microtubule Source: UniProtKB-KW
- spindle Source: UniProtKB
Cytosol
- cytosol Source: UniProtKB
Plasma Membrane
Other locations
- ciliary tip Source: UniProtKB
- perinuclear region of cytoplasm Source: UniProtKB-SubCell
Keywords - Cellular componenti
Cell membrane, Cell projection, Cytoplasm, Cytoskeleton, Membrane, MicrotubulePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000326149 | 1 – 953 | Ubiquitin carboxyl-terminal hydrolase CYLDAdd BLAST | 953 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 384 | PhosphoserineBy similarity | 1 | |
Modified residuei | 415 | PhosphoserineCombined sources | 1 | |
Modified residuei | 419 | PhosphoserineCombined sources | 1 |
Post-translational modificationi
Keywords - PTMi
Phosphoprotein, Ubl conjugationProteomic databases
PaxDbi | Q66H62 |
PRIDEi | Q66H62 |
PTM databases
iPTMneti | Q66H62 |
PhosphoSitePlusi | Q66H62 |
Interactioni
Subunit structurei
Interacts (via CAP-Gly domain) with IKBKG/NEMO (via proline-rich C-terminal region).
Interacts with TRAF2 and TRIP.
Interacts with PLK1, DVL1, DVL3, MAVS, TBK1, IKKE and DDX58.
Interacts (via CAP-Gly domain) with microtubules.
Interacts with HDAC6 and BCL3 (By similarity).
Interacts with MAP3K7.
Identified in a complex with TRAF6 and SQSTM1 (By similarity).
Interacts with CEP350.
Interacts with RNF31; the interaction is indirect and is mediated via SPATA2.
Interacts with SPATA2 (via the PUB domain); the interaction is direct and recruits CYLD to the LUBAC complex, thereby regulating TNF-alpha-induced necroptosis (By similarity).
By similarityGO - Molecular functioni
- proline-rich region binding Source: RGD
- protein kinase binding Source: RGD
Protein-protein interaction databases
BioGRIDi | 260280, 3 interactors |
STRINGi | 10116.ENSRNOP00000018888 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 153 – 198 | CAP-Gly 1PROSITE-ProRule annotationAdd BLAST | 46 | |
Domaini | 253 – 286 | CAP-Gly 2PROSITE-ProRule annotationAdd BLAST | 34 | |
Domaini | 489 – 532 | CAP-Gly 3PROSITE-ProRule annotationAdd BLAST | 44 | |
Domaini | 589 – 947 | USPAdd BLAST | 359 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 106 – 590 | Interaction with TRIPBy similarityAdd BLAST | 485 | |
Regioni | 391 – 466 | Interaction with TRAF2By similarityAdd BLAST | 76 | |
Regioni | 467 – 681 | Interaction with IKBKG/NEMOBy similarityAdd BLAST | 215 | |
Regioni | 778 – 830 | B-boxBy similarityAdd BLAST | 53 |
Sequence similaritiesi
Keywords - Domaini
RepeatPhylogenomic databases
eggNOGi | KOG3556, Eukaryota |
InParanoidi | Q66H62 |
OrthoDBi | 119442at2759 |
PhylomeDBi | Q66H62 |
Family and domain databases
Gene3Di | 2.30.30.190, 3 hits |
InterProi | View protein in InterPro IPR036859, CAP-Gly_dom_sf IPR000938, CAP-Gly_domain IPR038765, Papain-like_cys_pep_sf IPR001394, Peptidase_C19_UCH IPR018200, USP_CS IPR028889, USP_dom |
Pfami | View protein in Pfam PF01302, CAP_GLY, 2 hits PF00443, UCH, 1 hit |
SMARTi | View protein in SMART SM01052, CAP_GLY, 3 hits |
SUPFAMi | SSF54001, SSF54001, 1 hit SSF74924, SSF74924, 3 hits |
PROSITEi | View protein in PROSITE PS00845, CAP_GLY_1, 1 hit PS50245, CAP_GLY_2, 2 hits PS00972, USP_1, 1 hit PS50235, USP_3, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 2 potential isoforms that are computationally mapped.Show allAlign All
10 20 30 40 50
MSSGLWNQEK VTSPYWEERL FYLLLQECSV TDKQTQKLLR VPKGSIGQYI
60 70 80 90 100
QDRSVGHSRV PSAKGKKNQI GLKILEQPHA VLFVDEKDVV EINEKFTELL
110 120 130 140 150
LAITNCEERL SLFRNRIRLS KGLQVDVGSP VRVQLRSGEE KFPGVVRFRG
160 170 180 190 200
PLLAERTVSG IFFGVELLEE GRGQGFTDGV YQGKQLFQCD EDCGVFVALD
210 220 230 240 250
KLELIEDDDN GLESDFAGPG DTVQVEPPPL EINSRVSLKV GESTESGTVI
260 270 280 290 300
FCDVLPGKES LGYFVGVDMD NPIGNWDGRF DGVQLCSFAS VESTVLLHIN
310 320 330 340 350
DIIPDSVTQE RRPPKLAFMS RGVGDKGSSS HNKPKVTGST SDPGSRNRSE
360 370 380 390 400
LFYTLNGSSV DSQQQSKSKN PWYIDEVAED PAKSLTEMSS DFGHSSPPPQ
410 420 430 440 450
PPSMNSLSSE NRFHSLPFSL TKMPNTNGSM AHSPLSLSVQ SVMGELNSTP
460 470 480 490 500
VQESPPMPSS SGNAHGLEVG SLAEVKENPP FYGVIRWIGQ PPGLSDVLAG
510 520 530 540 550
LELEDECAGC TDGTFRGTRY FTCALKKALF VKLKSCRPDS RFASLQPVSN
560 570 580 590 600
QIERCNSLAF GGYLSEVVEE NTPPKMEKEG LEIMIGKKKG IQGHYNSCYL
610 620 630 640 650
DSTLFCLFAF SSALDTVLLR PKEKNDVEYY SETQELLRTE IVNPLRIYGY
660 670 680 690 700
VCATKIMKLR KILEKVEAAS GFTSEEKDPE EFLNILFHDI LRVEPLLKIR
710 720 730 740 750
SAGQKVQDCN FYQIFMEKNE KVGVPTIQQL LEWSFINSNL KFAEAPSCLI
760 770 780 790 800
IQMPRFGKDF KLFKKIFPSL ELNITDLLED TPRQCRICGG LAMYECRECY
810 820 830 840 850
DDPDISAGKI KQFCKTCSTQ VHLHPRRLNH TYHPVSLPKD LPDWDWRHGC
860 870 880 890 900
IPCQKMELFA VLCIETSHYV AFVKYGKDDS AWLFFDSMAD RDGGQNGFNI
910 920 930 940 950
PQVTPCPEVG EYLKMSLEDL HSLDSRRIQG CARRLLCDAY MCMYQSPTMS
LYK
Computationally mapped potential isoform sequencesi
There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketF1LPJ6 | F1LPJ6_RAT | Deubiquitinating enzyme CYLD | Cyld | 950 | Annotation score: | ||
Q6TXJ6 | Q6TXJ6_RAT | 40S ribosomal protein S3a | Cyld LOC100362727, RPS3A | 454 | Annotation score: |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | BC082001 mRNA Translation: AAH82001.1 |
RefSeqi | NP_001017380.1, NM_001017380.1 |
Genome annotation databases
GeneIDi | 312937 |
KEGGi | rno:312937 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | BC082001 mRNA Translation: AAH82001.1 |
RefSeqi | NP_001017380.1, NM_001017380.1 |
3D structure databases
SMRi | Q66H62 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 260280, 3 interactors |
STRINGi | 10116.ENSRNOP00000018888 |
Protein family/group databases
MEROPSi | C67.001 |
PTM databases
iPTMneti | Q66H62 |
PhosphoSitePlusi | Q66H62 |
Proteomic databases
PaxDbi | Q66H62 |
PRIDEi | Q66H62 |
Genome annotation databases
GeneIDi | 312937 |
KEGGi | rno:312937 |
Organism-specific databases
CTDi | 1540 |
RGDi | 1308346, Cyld |
Phylogenomic databases
eggNOGi | KOG3556, Eukaryota |
InParanoidi | Q66H62 |
OrthoDBi | 119442at2759 |
PhylomeDBi | Q66H62 |
Enzyme and pathway databases
Reactomei | R-RNO-168638, NOD1/2 Signaling Pathway R-RNO-5357786, TNFR1-induced proapoptotic signaling R-RNO-5357905, Regulation of TNFR1 signaling R-RNO-5357956, TNFR1-induced NFkappaB signaling pathway R-RNO-5689880, Ub-specific processing proteases |
Miscellaneous databases
PROi | PR:Q66H62 |
Family and domain databases
Gene3Di | 2.30.30.190, 3 hits |
InterProi | View protein in InterPro IPR036859, CAP-Gly_dom_sf IPR000938, CAP-Gly_domain IPR038765, Papain-like_cys_pep_sf IPR001394, Peptidase_C19_UCH IPR018200, USP_CS IPR028889, USP_dom |
Pfami | View protein in Pfam PF01302, CAP_GLY, 2 hits PF00443, UCH, 1 hit |
SMARTi | View protein in SMART SM01052, CAP_GLY, 3 hits |
SUPFAMi | SSF54001, SSF54001, 1 hit SSF74924, SSF74924, 3 hits |
PROSITEi | View protein in PROSITE PS00845, CAP_GLY_1, 1 hit PS50245, CAP_GLY_2, 2 hits PS00972, USP_1, 1 hit PS50235, USP_3, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | CYLD_RAT | |
Accessioni | Q66H62Primary (citable) accession number: Q66H62 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | March 18, 2008 |
Last sequence update: | October 11, 2004 | |
Last modified: | April 7, 2021 | |
This is version 122 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Peptidase families
Classification of peptidase families and list of entries - SIMILARITY comments
Index of protein domains and families