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Protein

Phosphatidylglycerophosphatase and protein-tyrosine phosphatase 1

Gene

Ptpmt1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Lipid phosphatase which dephosphorylates phosphatidylglycerophosphate (PGP) to phosphatidylglycerol (PG) (PubMed:21641550, PubMed:21730175). PGP is an essential intermediate in the biosynthetic pathway of cardiolipin, a mitochondrial-specific phospholipid regulating the membrane integrity and activities of the organelle (PubMed:21641550). Has also been shown to display phosphatase activity toward phosphoprotein substrates, specifically mediates dephosphorylation of mitochondrial proteins, thereby playing an essential role in ATP production (By similarity). Has probably a preference for proteins phosphorylated on Ser and/or Thr residues compared to proteins phosphorylated on Tyr residues (By similarity). Probably involved in regulation of insulin secretion in pancreatic beta cells (By similarity). May prevent intrinsic apoptosis, probably by regulating mitochondrial membrane integrity (By similarity).By similarity2 Publications

Caution

Was originally (PubMed:15247229) thought to have phosphatidylinositol 5-phosphatase activity, however, it was later shown (PubMed:16039589) that it is probably not the case in vivo.2 Publications

Catalytic activityi

Phosphatidylglycerophosphate + H2O = phosphatidylglycerol + phosphate.2 Publications
Protein tyrosine phosphate + H2O = protein tyrosine + phosphate.PROSITE-ProRule annotation
[a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.By similarity

Pathwayi: phosphatidylglycerol biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes phosphatidylglycerol from CDP-diacylglycerol.1 Publication
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. CDP-diacylglycerol--glycerol-3-phosphate 3-phosphatidyltransferase, mitochondrial (Pgs1), CDP-diacylglycerol--glycerol-3-phosphate 3-phosphatidyltransferase (Pgs1), CDP-diacylglycerol--glycerol-3-phosphate 3-phosphatidyltransferase (Pgs1), CDP-diacylglycerol--glycerol-3-phosphate 3-phosphatidyltransferase (Pgs1), CDP-diacylglycerol--glycerol-3-phosphate 3-phosphatidyltransferase (Pgs1)
  2. Phosphatidylglycerophosphatase and protein-tyrosine phosphatase 1 (Ptpmt1)
This subpathway is part of the pathway phosphatidylglycerol biosynthesis, which is itself part of Phospholipid metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes phosphatidylglycerol from CDP-diacylglycerol, the pathway phosphatidylglycerol biosynthesis and in Phospholipid metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei132Phosphocysteine intermediatePROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase, Protein phosphatase
Biological processLipid biosynthesis, Lipid metabolism, Phospholipid biosynthesis, Phospholipid metabolism

Enzyme and pathway databases

UniPathwayi
UPA00084;UER00504

Chemistry databases

SwissLipidsiSLP:000000643

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphatidylglycerophosphatase and protein-tyrosine phosphatase 1 (EC:3.1.3.272 Publications)
Alternative name(s):
PTEN-like phosphatase
Phosphoinositide lipid phosphatase
Protein-tyrosine phosphatase mitochondrial 1 (EC:3.1.3.16By similarity, EC:3.1.3.48PROSITE-ProRule annotation)
Gene namesi
Name:Ptpmt1
Synonyms:Plip
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:1913711 Ptpmt1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Membrane, Mitochondrion, Mitochondrion inner membrane

Pathology & Biotechi

Disruption phenotypei

Mice die prior to E8.5.1 Publication

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi2 – 37Missing : Loss of mitochondrion localization. 1 PublicationAdd BLAST36
Mutagenesisi2 – 19Missing : Does not affect mitochondrion localization. 1 PublicationAdd BLAST18
Mutagenesisi73E → A: Fails to dephosphorylate PGP in vitro. 1 Publication1
Mutagenesisi76E → A: Fails to dephosphorylate PGP in vitro. 1 Publication1
Mutagenesisi101D → A: Fails to dephosphorylate PGP in vitro. 1 Publication1
Mutagenesisi132C → S: Fails to dephosphorylate PGP in vitro. Does not affect level of Akt phosphorylation. 3 Publications1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 31MitochondrionSequence analysisAdd BLAST31
ChainiPRO_000002542432 – 193Phosphatidylglycerophosphatase and protein-tyrosine phosphatase 1Add BLAST162

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei85N6-succinyllysineCombined sources1

Proteomic databases

EPDiQ66GT5
MaxQBiQ66GT5
PaxDbiQ66GT5
PeptideAtlasiQ66GT5
PRIDEiQ66GT5

PTM databases

iPTMnetiQ66GT5
PhosphoSitePlusiQ66GT5

Expressioni

Tissue specificityi

Predominantly expressed in testis. Expressed at lower level in heart, brain, spleen, lung, liver, skeletal muscle, kidney, bone marrow, eye, lymph node, smooth muscle, prostate, thymus, stomach and uterus.1 Publication

Gene expression databases

CleanExiMM_PTPMT1

Interactioni

Subunit structurei

Interacts with STYXL1; the interaction inhibits PTPMT1 catalytic activity.By similarity

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000077036

Structurei

Secondary structure

1193
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliQ66GT5
SMRiQ66GT5
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ66GT5

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini109 – 177Tyrosine-protein phosphataseAdd BLAST69

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG1719 Eukaryota
ENOG4111IMR LUCA
HOVERGENiHBG079822
InParanoidiQ66GT5
KOiK14165

Family and domain databases

Gene3Di3.90.190.10, 1 hit
InterProiView protein in InterPro
IPR000340 Dual-sp_phosphatase_cat-dom
IPR024950 DUSP
IPR029021 Prot-tyrosine_phosphatase-like
IPR016130 Tyr_Pase_AS
IPR000387 TYR_PHOSPHATASE_dom
IPR020422 TYR_PHOSPHATASE_DUAL_dom
PANTHERiPTHR10159 PTHR10159, 1 hit
PfamiView protein in Pfam
PF00782 DSPc, 1 hit
SMARTiView protein in SMART
SM00195 DSPc, 1 hit
SUPFAMiSSF52799 SSF52799, 1 hit
PROSITEiView protein in PROSITE
PS00383 TYR_PHOSPHATASE_1, 1 hit
PS50056 TYR_PHOSPHATASE_2, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q66GT5-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MAASAWLEAG LARVLFYPTL LYTVFRGRVR GPAHRDWYHR IDHTVLLGAL
60 70 80 90 100
PLKNMTRRLV LDENVRGVIT MNEEYETRFL CNTSKEWKKA GVEQLRLSTV
110 120 130 140 150
DMTGVPTLAN LHKGVQFALK YQALGQCVYV HCKAGRSRSA TMVAAYLIQV
160 170 180 190
HNWSPEEAIE AIAKIRSHIS IRPSQLEVLK EFHKEITARA AKN
Length:193
Mass (Da):21,943
Last modified:October 11, 2004 - v1
Checksum:i740D39798855D439
GO

Sequence cautioni

The sequence AAH26750 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated
The sequence BAB28400 differs from that shown. Reason: Frameshift at position 36.Curated
The sequence BAB29504 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK012674 mRNA Translation: BAB28400.1 Frameshift.
AK014691 mRNA Translation: BAB29504.1 Different initiation.
BC026750 mRNA Translation: AAH26750.1 Different initiation.
BK005540 mRNA Translation: DAA05585.1
RefSeqiNP_079852.1, NM_025576.2
UniGeneiMm.23926

Genome annotation databases

GeneIDi66461
KEGGimmu:66461

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK012674 mRNA Translation: BAB28400.1 Frameshift.
AK014691 mRNA Translation: BAB29504.1 Different initiation.
BC026750 mRNA Translation: AAH26750.1 Different initiation.
BK005540 mRNA Translation: DAA05585.1
RefSeqiNP_079852.1, NM_025576.2
UniGeneiMm.23926

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3RGOX-ray1.93A37-193[»]
3RGQX-ray2.05A36-191[»]
ProteinModelPortaliQ66GT5
SMRiQ66GT5
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000077036

Chemistry databases

SwissLipidsiSLP:000000643

PTM databases

iPTMnetiQ66GT5
PhosphoSitePlusiQ66GT5

Proteomic databases

EPDiQ66GT5
MaxQBiQ66GT5
PaxDbiQ66GT5
PeptideAtlasiQ66GT5
PRIDEiQ66GT5

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi66461
KEGGimmu:66461

Organism-specific databases

CTDi114971
MGIiMGI:1913711 Ptpmt1

Phylogenomic databases

eggNOGiKOG1719 Eukaryota
ENOG4111IMR LUCA
HOVERGENiHBG079822
InParanoidiQ66GT5
KOiK14165

Enzyme and pathway databases

UniPathwayi
UPA00084;UER00504

Miscellaneous databases

EvolutionaryTraceiQ66GT5
PROiPR:Q66GT5
SOURCEiSearch...

Gene expression databases

CleanExiMM_PTPMT1

Family and domain databases

Gene3Di3.90.190.10, 1 hit
InterProiView protein in InterPro
IPR000340 Dual-sp_phosphatase_cat-dom
IPR024950 DUSP
IPR029021 Prot-tyrosine_phosphatase-like
IPR016130 Tyr_Pase_AS
IPR000387 TYR_PHOSPHATASE_dom
IPR020422 TYR_PHOSPHATASE_DUAL_dom
PANTHERiPTHR10159 PTHR10159, 1 hit
PfamiView protein in Pfam
PF00782 DSPc, 1 hit
SMARTiView protein in SMART
SM00195 DSPc, 1 hit
SUPFAMiSSF52799 SSF52799, 1 hit
PROSITEiView protein in PROSITE
PS00383 TYR_PHOSPHATASE_1, 1 hit
PS50056 TYR_PHOSPHATASE_2, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiPTPM1_MOUSE
AccessioniPrimary (citable) accession number: Q66GT5
Secondary accession number(s): Q9CSJ8, Q9D622
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 16, 2005
Last sequence update: October 11, 2004
Last modified: July 18, 2018
This is version 112 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  4. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
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Main funding by: National Institutes of Health

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