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Entry version 94 (31 Jul 2019)
Sequence version 1 (01 Nov 1996)
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Protein

RNA-directed RNA polymerase L

Gene

L

Organism
Sudan ebolavirus (strain Maleo-79) (SEBOV) (Sudan Ebola virus)
Status
Reviewed-Annotation score:

Annotation score:4 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the ‘correct annotation’ for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
-Protein inferred from homologyi <p>This indicates the type of evidence that supports the existence of the protein. Note that the ‘protein existence’ evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

RNA-directed RNA polymerase that catalyzes the transcription of viral mRNAs, their capping and polyadenylation. The template is composed of the viral RNA tightly encapsidated by the nucleoprotein (N). The viral polymerase binds to the genomic RNA at the 3' leader promoter, and transcribes subsequently all viral mRNAs with a decreasing efficiency. The first gene is the most transcribed, and the last the least transcribed. The viral phosphoprotein acts as a processivity factor. Capping is concommitant with initiation of mRNA transcription. Indeed, a GDP polyribonucleotidyl transferase (PRNTase) adds the cap structure when the nascent RNA chain length has reached few nucleotides. Ribose 2'-O methylation of viral mRNA cap precedes and facilitates subsequent guanine-N-7 methylation, both activities being carried by the viral polymerase. Polyadenylation of mRNAs occur by a stuttering mechanism at a slipery stop site present at the end viral genes. After finishing transcription of a mRNA, the polymerase can resume transcription of the downstream gene.By similarity
RNA-directed RNA polymerase that catalyzes the replication of viral genomic RNA. The template is composed of the viral RNA tightly encapsidated by the nucleoprotein (N). The replicase mode is dependent on intracellular N protein concentration. In this mode, the polymerase replicates the whole viral genome without recognizing transcriptional signals, and the replicated genome is not caped or polyadenylated.By similarity

<p>This subsection of the <a href="http://www.uniprot.org/help/function_section">Function</a> section describes the catalytic activity of an enzyme, i.e. a chemical reaction that the enzyme catalyzes.<p><a href='/help/catalytic_activity' target='_top'>More...</a></p>Catalytic activityi

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

<p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

Molecular functionMethyltransferase, Multifunctional enzyme, Nucleotidyltransferase, RNA-directed RNA polymerase, Transferase
Biological processmRNA capping, mRNA processing, Viral RNA replication
LigandATP-binding, Nucleotide-binding, S-adenosyl-L-methionine

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
Recommended name:
RNA-directed RNA polymerase L
Short name:
Protein L
Alternative name(s):
Large structural protein
Replicase
Transcriptase
Including the following 4 domains:
RNA-directed RNA polymerase (EC:2.7.7.48By similarity)
mRNA (guanine-N(7)-)-methyltransferase (EC:2.1.1.56By similarity)
GDP polyribonucleotidyltransferase (EC:2.7.7.88By similarity)
Cap-specific mRNA (nucleoside-2'-O-)-methyltransferase 2 (EC:2.1.1.296By similarity)
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: ‘Name’, ‘Synonyms’, ‘Ordered locus names’ and ‘ORF names’.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
Name:L
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiSudan ebolavirus (strain Maleo-79) (SEBOV) (Sudan Ebola virus)
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the ‘taxonomic identifier’ or ‘taxid’.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri128949 [NCBI]
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiVirusesRiboviriaNegarnaviricotaHaploviricotinaMonjiviricetesMononegaviralesFiloviridaeEbolavirus
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section only exists in viral entries and indicates the host(s) either as a specific organism or taxonomic group of organisms that are susceptible to be infected by a virus.<p><a href='/help/virus_host' target='_top'>More...</a></p>Virus hostiEpomops franqueti (Franquet's epauleted fruit bat) [TaxID: 77231]
Homo sapiens (Human) [TaxID: 9606]
Myonycteris torquata (Little collared fruit bat) [TaxID: 77243]

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Host cytoplasm, Virion

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the ‘PTM / Processing’ section describes the extent of a polypeptide chain in the mature protein following processing.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_00002221681 – 2210RNA-directed RNA polymerase LAdd BLAST2210

Proteomic databases

PRoteomics IDEntifications database

More...
PRIDEi
Q66802

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/family_and_domains_section">Family and Domains</a> section describes the position and type of a domain, which is defined as a specific combination of secondary structures organized into a characteristic three-dimensional structure or fold.<p><a href='/help/domain' target='_top'>More...</a></p>Domaini626 – 810RdRp catalyticPROSITE-ProRule annotationAdd BLAST185
Domaini1802 – 2000Mononegavirus-type SAM-dependent 2'-O-MTasePROSITE-ProRule annotationAdd BLAST199

Family and domain databases

Integrated resource of protein families, domains and functional sites

More...
InterProi
View protein in InterPro
IPR039736 L_poly_C
IPR026890 Mononeg_mRNAcap
IPR014023 Mononeg_RNA_pol_cat
IPR025786 Mononega_L_MeTrfase
IPR017235 RNA-dir_pol_L_filovirus

Pfam protein domain database

More...
Pfami
View protein in Pfam
PF14318 Mononeg_mRNAcap, 1 hit
PF00946 Mononeg_RNA_pol, 1 hit

PIRSF; a whole-protein classification database

More...
PIRSFi
PIRSF037548 RNA_pol_Filoviridae, 1 hit

TIGRFAMs; a protein family database

More...
TIGRFAMsi
TIGR04198 paramyx_RNAcap, 1 hit

PROSITE; a protein domain and family database

More...
PROSITEi
View protein in PROSITE
PS50526 RDRP_SSRNA_NEG_NONSEG, 1 hit
PS51590 SAM_MT_MNV_L, 1 hit

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence_length">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequencei

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences_section">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical_and_isoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

Q66802-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MMATQHTQYP DARLSSPIVL DQCDLVTRAC GLYSEYSLNP KLRTCRLPKH
60 70 80 90 100
IYRLKYDAIV LRFISDVPVA TIPIDYIAPM LINVLADSKN APLEPPCLSF
110 120 130 140 150
LDEIVNYTVQ DAAFLNYYMN QIKTQEGVIT DQLKQNIRRV IHKNRYLSAL
160 170 180 190 200
FFWHDLSILT RRGRMNRGNV RSTWFVTNEV VDILGYGDYI FWKIPIALLP
210 220 230 240 250
MNSANVPHAS TDWYQPNIFK EAIQGHTHII SVSTAEVLIM CKDLVTSRFN
260 270 280 290 300
TLLIAELARL EDPVSADYPL VDDIQSLYNA GDYLLSILGS EGYQIIKYLE
310 320 330 340 350
PLCLAKIQLC SQYTERKGRF LTQMHLAVIQ TLRELLLNRG LKKSQLSKIR
360 370 380 390 400
EFHQLLLRLR STPQQLCELF SIQKHWGHPV LHSEKAIQKV KNHATVLKAL
410 420 430 440 450
RPIIIFETYC VFKYSVAKHF FDSQGTWYSV ISDRCLTPGL NSYIRRNQFP
460 470 480 490 500
PLPMIKDLLW EFYHLDHPPL FSTKIISDLS IFIKDRATAV EQTCWDAVFE
510 520 530 540 550
PNVLGYSPPY RFNTKRVPEQ FLEQEDFSIE SVLQYAQELR YLLPQNRNFS
560 570 580 590 600
FSLKEKELNV GRTFGKLPYL TRNVQTLCEA LLADGLAKAF PSNMMVVTER
610 620 630 640 650
EQKESLLHQA SWHHTSDDFG EHATVRGSSF VTDLEKYNLA FRYEFTAPFI
660 670 680 690 700
KYCNQCYGVR NVFDWMHFLI PQCYMHVSDY YNPPHNVTLE NREYPPEGPS
710 720 730 740 750
AYRGHLGGIE GLQQKLWTSI SCAQISLVEI KTGFKLRSAV MGDNQCITVL
760 770 780 790 800
SVFPLESSPN EQERCAEDNA ARVAASLAKV TSACGIFLKP DETFVHSGFI
810 820 830 840 850
YFGPKQYLNG IQLPQSLKTA ARMAPLSDAI FDDLQGTLAS IGTAFERSIS
860 870 880 890 900
ETRHILPSRV AAAFHTYFSV RILQHHHLGF HKGSDLGQLA INKPLDFGTI
910 920 930 940 950
ALSLAVPQVL GGLSFLNPEK CLYRNLGDPV TSGLFQLKHY LSMVGMSDIF
960 970 980 990 1000
HALVAKSPGN CSAIDFVLNP GGLNVPGSQD LTSFLRQIVR RSITLSARNK
1010 1020 1030 1040 1050
LINTLFHASA DLEDELVCKW LLSSTPVMSR FAADIFSRTP SGKRLQILGY
1060 1070 1080 1090 1100
LEGTRTLLAS KMISNNAETP ILERLRKITL QRWNLWFSYL DHCDSALMEA
1110 1120 1130 1140 1150
IQPIRCTVDI AQILREYSWA HILGGRQLIG ATLPCIPEQF QTTWLKPYEQ
1160 1170 1180 1190 1200
CVECSSTNNS SPYVSVALKR NVVSAWPDAS RLGWTIGDGI PYIGSRTEDK
1210 1220 1230 1240 1250
IGQPAIKPRC PSAALREAIE LTSRLTWVTQ GSANSDQLIR PFLEARVNLS
1260 1270 1280 1290 1300
VQEILQMTPS HYSGNIVHRY NDQYSPHSFM ANRMSNTATR LMVSTNTLGE
1310 1320 1330 1340 1350
FSGGGQAARD SNIIFQNVIN FAVALYDIRF RNTCTSSIQY HRAHIHLTDC
1360 1370 1380 1390 1400
CTREVPAQYL TYTTTLNLDL SKYRNNELIY DSEPLRGGLN CNLSIDSPLM
1410 1420 1430 1440 1450
KGPRLNIIED DLIRLPHLSG WELAKTVLQS IISDSSNSST DPISSGETRS
1460 1470 1480 1490 1500
FTTHFLTYPK IGLLYSFGAL ISFYLGNTIL CTKKIGLTEF LYYLQNQIHN
1510 1520 1530 1540 1550
LSHRSLRIFK PTFRHSSVMS RLMDIDPNFS IYIGGTAGDR GLSDAARLFL
1560 1570 1580 1590 1600
RIAISTFLSF VEEWVIFRKA NIPLWVVYPL EGQRPDPPGE FLNRVKSLIV
1610 1620 1630 1640 1650
GIEDDKNKGS ILSRSEEKCS SNLVYNCKST ASNFFHASLA YWRGRHRPKK
1660 1670 1680 1690 1700
TIGATKATTA PHIILPLGNS DRPPGLDLNQ SNDTFIPTRI KQIVQGDSRN
1710 1720 1730 1740 1750
DRTTTTRLPP QSRSTPTSAT EPPTKIYEGS TTYRGKSTDT HLDEGHNAKE
1760 1770 1780 1790 1800
FPFNPHRLVV PFFKLTKDGE YSIEPSPEES RSNIKGLLQH LRTMVDTTIY
1810 1820 1830 1840 1850
CRFTGIVSSM HYKLDEVLWE YNKFESAVTL AEGEGSGALL LIQKYGVKKL
1860 1870 1880 1890 1900
FLNTLATEHS IESEVISGYT TPRMLLSVMP RTHRGELEVI LNNSASQITD
1910 1920 1930 1940 1950
ITHRDWFSNQ KNRIPNDVDI ITMDAETTEN LDRSRLYEAV YTIICNHINP
1960 1970 1980 1990 2000
KTLKVVILKV FLSDLDGMCW INNYLAPMFG SGYLIKPITS SARSSEWYLC
2010 2020 2030 2040 2050
LSNLLSTLRT TQHQTQANCL HVVQCALQQQ VQRGSYWLSH LTKYTTSRLH
2060 2070 2080 2090 2100
NSYIAFGFPS LEKVLYHRYN LVDSRNGPLV SITRHLALLQ TEIRELVTDY
2110 2120 2130 2140 2150
NQLRQSRTQT YHFIKTSKGR ITKLVNDYLR FELVIRALKN NSTWHHELYL
2160 2170 2180 2190 2200
LPELIGVCHR FNHTRNCTCS ERFLVQTLYL HRMSDAEIKL MDRLTSLVNM
2210
FPEGFRSSSV
Length:2,210
Mass (Da):251,258
Last modified:November 1, 1996 - v1
<p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:iF50E6B624951AEA4
GO

Sequence databases

Select the link destinations:

EMBL nucleotide sequence database

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EMBLi

GenBank nucleotide sequence database

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GenBanki

DNA Data Bank of Japan; a nucleotide sequence database

More...
DDBJi
Links Updated
U23458 Genomic RNA Translation: AAA79970.1

<p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U23458 Genomic RNA Translation: AAA79970.1

3D structure databases

Database of comparative protein structure models

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ModBasei
Search...

SWISS-MODEL Interactive Workspace

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SWISS-MODEL-Workspacei
Submit a new modelling project...

Proteomic databases

PRIDEiQ66802

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

InterProiView protein in InterPro
IPR039736 L_poly_C
IPR026890 Mononeg_mRNAcap
IPR014023 Mononeg_RNA_pol_cat
IPR025786 Mononega_L_MeTrfase
IPR017235 RNA-dir_pol_L_filovirus
PfamiView protein in Pfam
PF14318 Mononeg_mRNAcap, 1 hit
PF00946 Mononeg_RNA_pol, 1 hit
PIRSFiPIRSF037548 RNA_pol_Filoviridae, 1 hit
TIGRFAMsiTIGR04198 paramyx_RNAcap, 1 hit
PROSITEiView protein in PROSITE
PS50526 RDRP_SSRNA_NEG_NONSEG, 1 hit
PS51590 SAM_MT_MNV_L, 1 hit

ProtoNet; Automatic hierarchical classification of proteins

More...
ProtoNeti
Search...

MobiDB: a database of protein disorder and mobility annotations

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MobiDBi
Search...

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This subsection of the ‘Entry information’ section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiL_EBOSM
<p>This subsection of the ‘Entry information’ section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called ‘Primary (citable) accession number’.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: Q66802
<p>This subsection of the ‘Entry information’ section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification (‘Last modified’). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical_and_isoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: November 1, 1996
Last modified: July 31, 2019
This is version 94 of the entry and version 1 of the sequence. See complete history.
<p>This subsection of the ‘Entry information’ section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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