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Protein

Ribonucleoside-diphosphate reductase large subunit

Gene

RIR1

Organism
Equine herpesvirus 2 (strain 86/87) (EHV-2)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Ribonucleoside-diphosphate reductase holoenzyme provides the precursors necessary for viral DNA synthesis. Allows virus growth in non-dividing cells, as well as reactivation from latency in infected hosts. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides.UniRule annotation

Catalytic activityi

2'-deoxyribonucleoside diphosphate + thioredoxin disulfide + H2O = ribonucleoside diphosphate + thioredoxin.UniRule annotation

Pathwayi: DNA replication

This protein is involved in the pathway DNA replication, which is part of Genetic information processing.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway DNA replication and in Genetic information processing.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei192SubstrateUniRule annotation1
Sitei208Important for hydrogen atom transferUniRule annotation1
Binding sitei238Substrate; via amide nitrogenUniRule annotation1
Active sitei408Proton acceptorUniRule annotation1
Active sitei410Cysteine radical intermediateUniRule annotation1
Active sitei412Proton acceptorUniRule annotation1
Sitei424Important for hydrogen atom transferUniRule annotation1
Sitei743Important for electron transferUniRule annotation1
Sitei744Important for electron transferUniRule annotation1
Sitei795Interacts with thioredoxin/glutaredoxinUniRule annotation1
Sitei798Interacts with thioredoxin/glutaredoxinUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
Biological processDNA replication
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00326

Names & Taxonomyi

Protein namesi
Recommended name:
Ribonucleoside-diphosphate reductase large subunitUniRule annotation (EC:1.17.4.1UniRule annotation)
Short name:
R1UniRule annotation
Alternative name(s):
Ribonucleotide reductase large subunitUniRule annotation
Gene namesi
Name:RIR1UniRule annotation
Ordered Locus Names:61
OrganismiEquine herpesvirus 2 (strain 86/87) (EHV-2)
Taxonomic identifieri82831 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stageHerpesviralesHerpesviridaeGammaherpesvirinaePercavirus
Virus hostiEquus caballus (Horse) [TaxID: 9796]
Proteomesi
  • UP000007083 Componenti: Genome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004059871 – 799Ribonucleoside-diphosphate reductase large subunitAdd BLAST799

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi208 ↔ 424Redox-activeUniRule annotation

Keywords - PTMi

Disulfide bond

Proteomic databases

PRIDEiQ66663

Expressioni

Keywords - Developmental stagei

Early protein

Interactioni

Subunit structurei

Heterotetramer composed of a homodimer of the large subunit (R1) and a homodimer of the small subunit (R2). Larger multisubunit protein complex are also active, composed of (R1)n(R2)n.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliQ66663
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni207 – 208Substrate bindingUniRule annotation2
Regioni408 – 412Substrate bindingUniRule annotation5
Regioni612 – 616Substrate bindingUniRule annotation5

Sequence similaritiesi

Belongs to the ribonucleoside diphosphate reductase large chain family.UniRule annotation

Phylogenomic databases

OrthoDBiVOG0900001D

Family and domain databases

HAMAPiMF_04026 HSV_RIR1, 1 hit
InterProiView protein in InterPro
IPR034717 HSV_RIR1
IPR013346 NrdE_NrdA
IPR000788 RNR_lg_C
IPR013509 RNR_lsu_N
PfamiView protein in Pfam
PF02867 Ribonuc_red_lgC, 1 hit
PF00317 Ribonuc_red_lgN, 1 hit
PRINTSiPR01183 RIBORDTASEM1
TIGRFAMsiTIGR02506 NrdE_NrdA, 1 hit

Sequencei

Sequence statusi: Complete.

Q66663-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAAAHLGMPV PVYMAEREED LALRKLIDTL KISAGWDLEA NRMAGRLYHF
60 70 80 90 100
VMGRRAPVDT RAYVTTFGDK LEKGVHRFLW DNARDIDDLC KDFQNGPEYE
110 120 130 140 150
RLISRGMLSA KRMYDTYVLR TEEGGGEGAV YESALQMYAR MAAFFTCQCF
160 170 180 190 200
AYPSLRKTVS EVEGGSGRME SGLDFFAYFF KILASQLVSC ATPVMRSAGL
210 220 230 240 250
RQSYLASCFI MNPDMSTEDK TLSAVFRDLS PLLCSKSGVG MNLTNFSSGG
260 270 280 290 300
KNVQSCLRLI NSQVEFCNDK NLRPVSVAAY MELWHEQIEE FLAAKLPENP
310 320 330 340 350
ERCQSIFQGV CVPGLFFRLY EQNPDSQWHL FSPKVGGHLA GLYGDKFDEE
360 370 380 390 400
YARLVRHGLY SSSLPAKSLM FALISAIIKT GSPYILSKDA INRHHWFETQ
410 420 430 440 450
GNAISYANLC AEVVQQPHEF TSTCNLANVC LPACLRARGE GEGAGAAGEG
460 470 480 490 500
EGADRLSPLT PDLEFCFDTL RAAVRAAVYM INASILGGVC PTPGVRVMQA
510 520 530 540 550
ERSAGIGVQG LADVFAKLGR GYMDAESALL DARIFEVMYY QAVRASNQLV
560 570 580 590 600
TVGGAPPHAN WKNSKLSRGE FHWESWGVQY DSLFIERQLW EELRESVTKH
610 620 630 640 650
GTFNSQFIAL MPTAGTSQLT GLSESFYPFY ANVSSKVSNK EEVMKPNITF
660 670 680 690 700
LERVSPSDVP LLKYHGGDVS KLPPPLAAKY RNFLTAFDYS PEDQIRRAAA
710 720 730 740 750
RSPFVDQSQS FSFFLKEANV KSASYVKNLI LLGHGLGLKT IMYYCRIQKQ
760 770 780 790
TSLTALECLQ CTESPDSGDG VGGYKGGDEE PRSPEHAQCE SPDRCLSCQ
Length:799
Mass (Da):88,581
Last modified:November 1, 1996 - v1
Checksum:iA88D2540357934F0
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U20824 Genomic DNA Translation: AAC13849.1
PIRiS55656
RefSeqiNP_042658.1, NC_001650.2

Genome annotation databases

GeneIDi1461039
KEGGivg:1461039

Similar proteinsi

Entry informationi

Entry nameiRIR1_EHV2
AccessioniPrimary (citable) accession number: Q66663
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 8, 2011
Last sequence update: November 1, 1996
Last modified: May 23, 2018
This is version 85 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

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