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Protein

Wings apart-like protein homolog

Gene

Wapl

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Regulator of sister chromatid cohesion in mitosis which negatively regulates cohesin association with chromatin. Involved in both sister chromatid cohesion during interphase and sister-chromatid resolution during early stages of mitosis. Couples DNA replication to sister chromatid cohesion. Cohesion ensures that chromosome partitioning is accurate in both meiotic and mitotic cells and plays an important role in DNA repair (By similarity).By similarity

GO - Biological processi

Keywordsi

Biological processCell cycle, Cell division, Mitosis

Enzyme and pathway databases

ReactomeiR-MMU-2467813 Separation of Sister Chromatids
R-MMU-2468052 Establishment of Sister Chromatid Cohesion
R-MMU-2470946 Cohesin Loading onto Chromatin
R-MMU-2500257 Resolution of Sister Chromatid Cohesion

Names & Taxonomyi

Protein namesi
Recommended name:
Wings apart-like protein homolog
Alternative name(s):
Dioxin-inducible factor 2
Short name:
DIF-2
WAPL cohesin release factorImported
Gene namesi
Name:WaplImported
Synonyms:Kiaa0261, Wapal
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 14

Organism-specific databases

MGIiMGI:2675859 Wapl

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Chromosome, Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002452331 – 1200Wings apart-like protein homologAdd BLAST1200

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei77PhosphoserineCombined sources1
Modified residuei168N6-acetyllysineBy similarity1
Modified residuei226PhosphoserineCombined sources1
Modified residuei228PhosphoserineBy similarity1
Modified residuei286PhosphothreonineCombined sources1
Modified residuei355PhosphoserineBy similarity1
Modified residuei388PhosphoserineBy similarity1
Modified residuei452PhosphoserineBy similarity1
Modified residuei468PhosphoserineCombined sources1
Modified residuei470PhosphoserineCombined sources1
Modified residuei914PhosphoserineBy similarity1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ65Z40
MaxQBiQ65Z40
PaxDbiQ65Z40
PRIDEiQ65Z40

PTM databases

iPTMnetiQ65Z40
PhosphoSitePlusiQ65Z40

Expressioni

Tissue specificityi

Highly expressed in large pachytene spermatocytes of testis. Down-regulated by dioxin in testis.1 Publication

Gene expression databases

BgeeiENSMUSG00000041408 Expressed in 248 organ(s), highest expression level in cleaving embryo
CleanExiMM_WAPAL
ExpressionAtlasiQ65Z40 baseline and differential
GenevisibleiQ65Z40 MM

Interactioni

Subunit structurei

Interacts with the cohesin complex throughout the cell cycle; interacts with both chromatin-bound and soluble pools of the complex. Interacts with RAD21; the interaction is direct (By similarity). Interacts with PDS5A; the interaction is direct, cohesin-dependent and competitive with CDCA5/SORORIN (By similarity). Interacts (via FGF motifs) with PDS5B; the interaction is direct (By similarity). Interacts with a SMC1 protein (SMC1A or SMC1B) and SMC3 (By similarity).By similarity

Protein-protein interaction databases

BioGridi230082, 6 interactors
IntActiQ65Z40, 1 interactor
STRINGi10090.ENSMUSP00000040232

Structurei

3D structure databases

ProteinModelPortaliQ65Z40
SMRiQ65Z40
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini635 – 1179WAPLPROSITE-ProRule annotationAdd BLAST545

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 668Mediates interaction with the cohesin complexBy similarityAdd BLAST668

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili266 – 293Sequence analysisAdd BLAST28
Coiled coili751 – 791Sequence analysisAdd BLAST41

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi73 – 75FGF motif 13
Motifi437 – 439FGF motif 23
Motifi462 – 464FGF motif 33

Sequence similaritiesi

Belongs to the WAPL family.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiKOG2152 Eukaryota
ENOG410XSDS LUCA
GeneTreeiENSGT00390000015768
HOGENOMiHOG000001563
HOVERGENiHBG088338
InParanoidiQ65Z40
OMAiVKMTSRF
OrthoDBiEOG091G042I
TreeFamiTF323477

Family and domain databases

Gene3Di1.25.10.10, 2 hits
InterProiView protein in InterPro
IPR011989 ARM-like
IPR016024 ARM-type_fold
IPR039874 WAPL
IPR022771 WAPL_C
IPR012502 WAPL_dom
PANTHERiPTHR22100 PTHR22100, 1 hit
PfamiView protein in Pfam
PF07814 WAPL, 1 hit
SUPFAMiSSF48371 SSF48371, 1 hit
PROSITEiView protein in PROSITE
PS51271 WAPL, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

This entry has 1 described isoform and 3 potential isoforms that are computationally mapped.Show allAlign All

Q65Z40-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MTSRFGKTYS RKGGNGSSKF DEVFSNKRTT LSTKWGETTF MAKLGQKRPN
60 70 80 90 100
FKPDIQEIPK KPKVEEEDTG DPFGFDSDDE SLPVSSKNLA QGKGSSYSES
110 120 130 140 150
SEAAQLEEVT SVFEANSKCS HVVGEDSFAS DRCLLVEDTL IGKEKSISRI
160 170 180 190 200
PEDNANKSSC TKLLTSDKVE NFSEEHEKNS HHFHKNAEDS TKKPNAETAV
210 220 230 240 250
ASEYKADETK ETNDTWNSQS GKRTESPSES CPVKGSVRTG LYEWDNDFED
260 270 280 290 300
IRSEDCILSL DNESLLEMKD EDLKNRIGGL ENLNETFEED IIQSVLRPSN
310 320 330 340 350
CRTYCRANKA RSSQGASNFD KLMDGTSQSL AKANSESSKD GLNQAKKGSA
360 370 380 390 400
SCGTSFRGTV GRTRDYTVLH PSCLSVCNVT IQDTMERSMD EFTASTPADL
410 420 430 440 450
GEAGRLRKKA DIATSKTTTR FRPSNTKSKK DVKLEFFGFE DHDETGGDEG
460 470 480 490 500
GSGSSNYKIK YFGFDDLSES EDDDDDDCQV ERKKDKKRTK TAPSPSQQPP
510 520 530 540 550
PESSDNSQDS QSSTNNAENL DFTEDLPGVP ESVKKPISKQ GDKSKENTRK
560 570 580 590 600
IFSGPKRSPT KAVYNARHWN HPDSEELPGP PIAKPQRVTV RLSSKEPNQK
610 620 630 640 650
DDGVFKAPAP PLKVIKTVTI PTQPYQEIVT ALKCRKEDKE LYTVVQHVKH
660 670 680 690 700
FNDVVEFGEN QEFTDDIEYL LSGLKSTQPL NTRCLSVISL ATKCAMPSFR
710 720 730 740 750
MHLRAHGMVA MVFKTLDDSQ HHQNLSLCTA ALMYILSRDR LNMDLDRASL
760 770 780 790 800
DLMIRLLELE QDASSAKLLN EKDMNKIKEK IRRLCETVHN KHLDLENITT
810 820 830 840 850
GHLAMETLLS LTSKRAGDWF KEELRLLGGL DHIVDKVKEC VDHLSRDDED
860 870 880 890 900
EEKLVASLWG AERCLRVLES VTVHNPENQS YLIAYKDSQL IISSAKALQH
910 920 930 940 950
CEDLIQQYNR AENSICVADS NPLPYQNVTN HVGKAVEDCM RAIIGVLLNL
960 970 980 990 1000
TNDNEWGSTK TGEQEGLIGT AMNCVLQVPK YLPQEQRFDI RVLGLGLLIN
1010 1020 1030 1040 1050
LVEYSARNRH CLVNMQTSCS FDSSFSSGEG DHSLRLAGQV HAVQALVQLF
1060 1070 1080 1090 1100
LERERAAQLA ESKTDELIKD APTTQHDKSG EWQETSGEIQ WVSTEKTDGA
1110 1120 1130 1140 1150
EEKQKKEEED EELDLNKALQ HAGKHMEDCI VASYTALLLG CLCQESPINV
1160 1170 1180 1190 1200
TTVREYLPEG DFSIMTEMLK KFLSFMNLTC AVGTTGQKSI SRVIEYLEHC
Length:1,200
Mass (Da):134,070
Last modified:July 27, 2011 - v2
Checksum:i7697FA31AABBC573
GO

Computationally mapped potential isoform sequencesi

There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
B7ZP47B7ZP47_MOUSE
Wapal protein
Wapl Wapal
1,194Annotation score:
F6YCH1F6YCH1_MOUSE
Wings apart-like protein homolog
Wapl Wapal
430Annotation score:
G3UXQ2G3UXQ2_MOUSE
Wings apart-like protein homolog
Wapl Wapal
160Annotation score:

Sequence cautioni

The sequence BAC97915 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti757L → V in BAD44717 (PubMed:15620708).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB167349 mRNA Translation: BAD44717.1
AK129105 mRNA Translation: BAC97915.1 Different initiation.
BC139434 mRNA Translation: AAI39435.1
CCDSiCCDS36880.1
RefSeqiNP_001004436.2, NM_001004436.4
NP_001288259.1, NM_001301330.1
UniGeneiMm.27957
Mm.437351
Mm.491215

Genome annotation databases

EnsembliENSMUST00000048263; ENSMUSP00000040232; ENSMUSG00000041408
ENSMUST00000169910; ENSMUSP00000130547; ENSMUSG00000041408
GeneIDi218914
KEGGimmu:218914
UCSCiuc007tbi.2 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB167349 mRNA Translation: BAD44717.1
AK129105 mRNA Translation: BAC97915.1 Different initiation.
BC139434 mRNA Translation: AAI39435.1
CCDSiCCDS36880.1
RefSeqiNP_001004436.2, NM_001004436.4
NP_001288259.1, NM_001301330.1
UniGeneiMm.27957
Mm.437351
Mm.491215

3D structure databases

ProteinModelPortaliQ65Z40
SMRiQ65Z40
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi230082, 6 interactors
IntActiQ65Z40, 1 interactor
STRINGi10090.ENSMUSP00000040232

PTM databases

iPTMnetiQ65Z40
PhosphoSitePlusiQ65Z40

Proteomic databases

EPDiQ65Z40
MaxQBiQ65Z40
PaxDbiQ65Z40
PRIDEiQ65Z40

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000048263; ENSMUSP00000040232; ENSMUSG00000041408
ENSMUST00000169910; ENSMUSP00000130547; ENSMUSG00000041408
GeneIDi218914
KEGGimmu:218914
UCSCiuc007tbi.2 mouse

Organism-specific databases

CTDi23063
MGIiMGI:2675859 Wapl
RougeiSearch...

Phylogenomic databases

eggNOGiKOG2152 Eukaryota
ENOG410XSDS LUCA
GeneTreeiENSGT00390000015768
HOGENOMiHOG000001563
HOVERGENiHBG088338
InParanoidiQ65Z40
OMAiVKMTSRF
OrthoDBiEOG091G042I
TreeFamiTF323477

Enzyme and pathway databases

ReactomeiR-MMU-2467813 Separation of Sister Chromatids
R-MMU-2468052 Establishment of Sister Chromatid Cohesion
R-MMU-2470946 Cohesin Loading onto Chromatin
R-MMU-2500257 Resolution of Sister Chromatid Cohesion

Miscellaneous databases

PROiPR:Q65Z40
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000041408 Expressed in 248 organ(s), highest expression level in cleaving embryo
CleanExiMM_WAPAL
ExpressionAtlasiQ65Z40 baseline and differential
GenevisibleiQ65Z40 MM

Family and domain databases

Gene3Di1.25.10.10, 2 hits
InterProiView protein in InterPro
IPR011989 ARM-like
IPR016024 ARM-type_fold
IPR039874 WAPL
IPR022771 WAPL_C
IPR012502 WAPL_dom
PANTHERiPTHR22100 PTHR22100, 1 hit
PfamiView protein in Pfam
PF07814 WAPL, 1 hit
SUPFAMiSSF48371 SSF48371, 1 hit
PROSITEiView protein in PROSITE
PS51271 WAPL, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiWAPL_MOUSE
AccessioniPrimary (citable) accession number: Q65Z40
Secondary accession number(s): B9EIG8, Q6ZQE9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 11, 2006
Last sequence update: July 27, 2011
Last modified: November 7, 2018
This is version 100 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
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Main funding by: National Institutes of Health

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