Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

tRNA(Ile)-lysidine synthase

Gene

tilS

Organism
Bacillus licheniformis (strain ATCC 14580 / DSM 13 / JCM 2505 / NBRC 12200 / NCIMB 9375 / NRRL NRS-1264 / Gibson 46)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Ligates lysine onto the cytidine present at position 34 of the AUA codon-specific tRNA(Ile) that contains the anticodon CAU, in an ATP-dependent manner. Cytidine is converted to lysidine, thus changing the amino acid specificity of the tRNA from methionine to isoleucine.UniRule annotation

Catalytic activityi

(tRNA(Ile2))-cytidine(34) + L-lysine + ATP = (tRNA(Ile2))-lysidine(34) + AMP + diphosphate + H2O.UniRule annotation

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi25 – 30ATPUniRule annotation6

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLigase
Biological processtRNA processing
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciBLIC279010:BLI_RS00415-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA(Ile)-lysidine synthaseUniRule annotation (EC:6.3.4.19UniRule annotation)
Alternative name(s):
tRNA(Ile)-2-lysyl-cytidine synthaseUniRule annotation
tRNA(Ile)-lysidine synthetaseUniRule annotation
Gene namesi
Name:tilSUniRule annotation
Ordered Locus Names:BLi00083, BL00503
OrganismiBacillus licheniformis (strain ATCC 14580 / DSM 13 / JCM 2505 / NBRC 12200 / NCIMB 9375 / NRRL NRS-1264 / Gibson 46)
Taxonomic identifieri279010 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
Proteomesi
  • UP000000606 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001816481 – 476tRNA(Ile)-lysidine synthaseAdd BLAST476

Proteomic databases

PRIDEiQ65PF4

Interactioni

Protein-protein interaction databases

STRINGi279010.BLi00083

Structurei

3D structure databases

ProteinModelPortaliQ65PF4
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domaini

The N-terminal region contains the highly conserved SGGXDS motif, predicted to be a P-loop motif involved in ATP binding.

Sequence similaritiesi

Belongs to the tRNA(Ile)-lysidine synthase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105D3U Bacteria
COG0037 LUCA
HOGENOMiHOG000236506
KOiK04075
OMAiAHCNFNL

Family and domain databases

CDDicd01992 PP-ATPase, 1 hit
Gene3Di3.40.50.620, 2 hits
HAMAPiMF_01161 tRNA_Ile_lys_synt, 1 hit
InterProiView protein in InterPro
IPR012796 Lysidine-tRNA-synth_C
IPR014729 Rossmann-like_a/b/a_fold
IPR011063 TilS/TtcA_N
IPR012094 tRNA_Ile_lys_synt
IPR012795 tRNA_Ile_lys_synt_N
IPR015262 tRNA_Ile_lys_synt_subst-bd
PANTHERiPTHR43033:SF1 PTHR43033:SF1, 1 hit
PfamiView protein in Pfam
PF01171 ATP_bind_3, 1 hit
PF09179 TilS, 1 hit
PF11734 TilS_C, 1 hit
SMARTiView protein in SMART
SM00977 TilS_C, 1 hit
TIGRFAMsiTIGR02433 lysidine_TilS_C, 1 hit
TIGR02432 lysidine_TilS_N, 1 hit

Sequencei

Sequence statusi: Complete.

Q65PF4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKSIEDFMKK YELSFDGVTL IAGVSGGPDS MALLHALHHT VPASATLIAA
60 70 80 90 100
HVDHMFRGEE SERDMRFVQD YCEAEGIQCE AVQIDVLAFA AENNLNKQAA
110 120 130 140 150
ARECRYAFFQ ELMKRHRAEY LVLGHHGDDQ VETMLMKMAK GTVGIGLAGI
160 170 180 190 200
QPKRRFDSGW LLRPFLKLSK DDLLAYCKEN RIPFRTDPSN SEDDYTRNRF
210 220 230 240 250
RHHVLPFLKK ESEDVHKRFQ SVSEFLTEDE LYLQALTKDK MNTVITSKSS
260 270 280 290 300
SGVEISIERL LALPMPLQRR GVHLILNYLY ENVPSAFSAH HIQVFLDWIS
310 320 330 340 350
QDGPSGSLDF PNGLKVAKSY HTCLFTFQRL QCENVSYHYE ISGAGEEELI
360 370 380 390 400
LPGGSSLHVS GPGSTRKLQG NDVFATSPKQ VQFPLYVRTR QNGDRVKLKG
410 420 430 440 450
MNGSKKVKDI FIDEKVPLSK RDSWPIVTDA VGNIIWIPGL KKTIFEELDV
460 470
TNNDRIVLQY RQHEKCRGLA KNETGY
Length:476
Mass (Da):53,986
Last modified:October 25, 2004 - v1
Checksum:iC9CB56F19042D53B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE017333 Genomic DNA Translation: AAU39060.1
CP000002 Genomic DNA Translation: AAU21715.1
RefSeqiWP_011197473.1, NC_006322.1

Genome annotation databases

EnsemblBacteriaiAAU21715; AAU21715; BL00503
AAU39060; AAU39060; BLi00083
GeneIDi3029360
KEGGibld:BLi00083
bli:BL00503
PATRICifig|279010.13.peg.73

Similar proteinsi

Entry informationi

Entry nameiTILS_BACLD
AccessioniPrimary (citable) accession number: Q65PF4
Secondary accession number(s): Q62ZU3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 15, 2005
Last sequence update: October 25, 2004
Last modified: June 20, 2018
This is version 93 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health