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Protein

L-lactate dehydrogenase

Gene

ldh

Organism
Bacillus licheniformis (strain ATCC 14580 / DSM 13 / JCM 2505 / NBRC 12200 / NCIMB 9375 / NRRL NRS-1264 / Gibson 46)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the conversion of lactate to pyruvate.UniRule annotation

Catalytic activityi

(S)-lactate + NAD+ = pyruvate + NADH.UniRule annotation

Activity regulationi

Allosterically activated by fructose 1,6-bisphosphate (FBP).UniRule annotation

Pathwayi: pyruvate fermentation to lactate

This protein is involved in step 1 of the subpathway that synthesizes (S)-lactate from pyruvate.UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. L-lactate dehydrogenase (ldh)
This subpathway is part of the pathway pyruvate fermentation to lactate, which is itself part of Fermentation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes (S)-lactate from pyruvate, the pathway pyruvate fermentation to lactate and in Fermentation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei17NAD; via amide nitrogenUniRule annotation1
Binding sitei38NADUniRule annotation1
Binding sitei43NADUniRule annotation1
Binding sitei69NADUniRule annotation1
Binding sitei86SubstrateUniRule annotation1
Binding sitei92SubstrateUniRule annotation1
Binding sitei147NADUniRule annotation1
Binding sitei157Allosteric activatorUniRule annotation1
Binding sitei172Allosteric activatorUniRule annotation1
Active sitei179Proton acceptorUniRule annotation1
Binding sitei233SubstrateUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi83 – 84NADUniRule annotation2
Nucleotide bindingi122 – 124NADUniRule annotation3

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAllosteric enzyme, Oxidoreductase
LigandNAD

Enzyme and pathway databases

UniPathwayi
UPA00554;UER00611

Names & Taxonomyi

Protein namesi
Recommended name:
L-lactate dehydrogenaseUniRule annotation (EC:1.1.1.27UniRule annotation)
Short name:
L-LDHUniRule annotation
Gene namesi
Name:ldhUniRule annotation
Ordered Locus Names:BLi00366, BL01703
OrganismiBacillus licheniformis (strain ATCC 14580 / DSM 13 / JCM 2505 / NBRC 12200 / NCIMB 9375 / NRRL NRS-1264 / Gibson 46)
Taxonomic identifieri279010 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
Proteomesi
  • UP000000606 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002375401 – 319L-lactate dehydrogenaseAdd BLAST319

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei224PhosphotyrosineUniRule annotation1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PRIDEiQ65NP0

Interactioni

Subunit structurei

Homotetramer.UniRule annotation

Protein-protein interaction databases

STRINGi279010.BLi00366

Structurei

3D structure databases

ProteinModelPortaliQ65NP0
SMRiQ65NP0
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni124 – 127Substrate bindingUniRule annotation4
Regioni152 – 155Substrate bindingUniRule annotation4

Sequence similaritiesi

Belongs to the LDH/MDH superfamily. LDH family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0039 LUCA
HOGENOMiHOG000213793
KOiK00016
OMAiMDLMQTA

Family and domain databases

Gene3Di3.90.110.10, 1 hit
HAMAPiMF_00488 Lactate_dehydrog, 1 hit
InterProiView protein in InterPro
IPR001557 L-lactate/malate_DH
IPR011304 L-lactate_DH
IPR018177 L-lactate_DH_AS
IPR022383 Lactate/malate_DH_C
IPR001236 Lactate/malate_DH_N
IPR015955 Lactate_DH/Glyco_Ohase_4_C
IPR036291 NAD(P)-bd_dom_sf
PfamiView protein in Pfam
PF02866 Ldh_1_C, 1 hit
PF00056 Ldh_1_N, 1 hit
PIRSFiPIRSF000102 Lac_mal_DH, 1 hit
PRINTSiPR00086 LLDHDRGNASE
SUPFAMiSSF51735 SSF51735, 1 hit
SSF56327 SSF56327, 1 hit
TIGRFAMsiTIGR01771 L-LDH-NAD, 1 hit
PROSITEiView protein in PROSITE
PS00064 L_LDH, 1 hit

Sequencei

Sequence statusi: Complete.

Q65NP0-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MRNQKVNRTV LIGAGFVGSS YAFTLINQGI TDELVIIDLN KDKAMGDVMD
60 70 80 90 100
LNHGKAFAPH PVKTWYGTYD DCKEADIVCV CAGANQKPGE TRLDLVEKNL
110 120 130 140 150
KIFKGIIGEV MASGFDGIFL VATNPVDILT YATWKFSGLP KERVIGSGTT
160 170 180 190 200
LDTARFRYLL SEYFGVAAHN AHGYIIGEHG DTELPVWSHA NIGGVPVSDL
210 220 230 240 250
LKRNEKYKAE DLDELFDNVK NAAYHIIEKK GATYYGVAMS LARITKAIYR
260 270 280 290 300
NEEAILTVSA HLDGEFGEND VYIGVPAVVG RCGAREIVEL DLNEKEKQQF
310
KHSAGVLKAI LKPHFELQA
Length:319
Mass (Da):35,111
Last modified:October 25, 2004 - v1
Checksum:i51D09BEDAAD3BBC6
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE017333 Genomic DNA Translation: AAU39324.1
CP000002 Genomic DNA Translation: AAU21970.1
RefSeqiWP_003178742.1, NC_006322.1

Genome annotation databases

EnsemblBacteriaiAAU21970; AAU21970; BL01703
AAU39324; AAU39324; BLi00366
GeneIDi3029440
KEGGibld:BLi00366
bli:BL01703

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE017333 Genomic DNA Translation: AAU39324.1
CP000002 Genomic DNA Translation: AAU21970.1
RefSeqiWP_003178742.1, NC_006322.1

3D structure databases

ProteinModelPortaliQ65NP0
SMRiQ65NP0
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi279010.BLi00366

Proteomic databases

PRIDEiQ65NP0

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAU21970; AAU21970; BL01703
AAU39324; AAU39324; BLi00366
GeneIDi3029440
KEGGibld:BLi00366
bli:BL01703

Phylogenomic databases

eggNOGiCOG0039 LUCA
HOGENOMiHOG000213793
KOiK00016
OMAiMDLMQTA

Enzyme and pathway databases

UniPathwayi
UPA00554;UER00611

Family and domain databases

Gene3Di3.90.110.10, 1 hit
HAMAPiMF_00488 Lactate_dehydrog, 1 hit
InterProiView protein in InterPro
IPR001557 L-lactate/malate_DH
IPR011304 L-lactate_DH
IPR018177 L-lactate_DH_AS
IPR022383 Lactate/malate_DH_C
IPR001236 Lactate/malate_DH_N
IPR015955 Lactate_DH/Glyco_Ohase_4_C
IPR036291 NAD(P)-bd_dom_sf
PfamiView protein in Pfam
PF02866 Ldh_1_C, 1 hit
PF00056 Ldh_1_N, 1 hit
PIRSFiPIRSF000102 Lac_mal_DH, 1 hit
PRINTSiPR00086 LLDHDRGNASE
SUPFAMiSSF51735 SSF51735, 1 hit
SSF56327 SSF56327, 1 hit
TIGRFAMsiTIGR01771 L-LDH-NAD, 1 hit
PROSITEiView protein in PROSITE
PS00064 L_LDH, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiLDH_BACLD
AccessioniPrimary (citable) accession number: Q65NP0
Secondary accession number(s): Q62Z38
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 30, 2006
Last sequence update: October 25, 2004
Last modified: October 10, 2018
This is version 106 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
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Main funding by: National Institutes of Health

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