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UniProtKB - Q65G92 (Q65G92_BACLD)
Protein
DNA polymerase I
Gene
polA
Organism
Bacillus licheniformis (strain ATCC 14580 / DSM 13 / JCM 2505 / CCUG 7422 / NBRC 12200 / NCIMB 9375 / NCTC 10341 / NRRL NRS-1264 / Gibson 46)
Status
Functioni
In addition to polymerase activity, this DNA polymerase exhibits 5'-3' exonuclease activity.
UniRule annotationCatalytic activityi
- a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) = diphosphate + DNA(n+1)UniRule annotationARBA annotationEC:2.7.7.7UniRule annotationARBA annotation
GO - Molecular functioni
- 3'-5' exonuclease activity Source: InterPro
- DNA binding Source: UniProtKB-UniRule
- DNA-directed DNA polymerase activity Source: UniProtKB-UniRule
GO - Biological processi
- DNA-dependent DNA replication Source: UniProtKB-UniRule
- DNA repair Source: UniProtKB-UniRule
Keywordsi
Molecular function | DNA-bindingUniRule annotationARBA annotation, DNA-directed DNA polymeraseUniRule annotationARBA annotation, Hydrolase, NucleaseARBA annotation, Nucleotidyltransferase, Transferase |
Biological process | DNA damage, DNA repairUniRule annotation, DNA replicationUniRule annotation |
Enzyme and pathway databases
BioCyci | BLIC279010:BLI_RS15135-MONOMER |
Names & Taxonomyi
Protein namesi | Recommended name: DNA polymerase IUniRule annotationARBA annotation (EC:2.7.7.7UniRule annotationARBA annotation) |
Gene namesi | Name:polAUniRule annotationImported Ordered Locus Names:BL00394Imported |
Organismi | Bacillus licheniformis (strain ATCC 14580 / DSM 13 / JCM 2505 / CCUG 7422 / NBRC 12200 / NCIMB 9375 / NCTC 10341 / NRRL NRS-1264 / Gibson 46)Imported |
Taxonomic identifieri | 279010 [NCBI] |
Taxonomic lineagei | Bacteria › Firmicutes › Bacilli › Bacillales › Bacillaceae › Bacillus › |
Proteomesi |
|
Interactioni
Subunit structurei
Single-chain monomer with multiple functions.
UniRule annotationARBA annotationProtein-protein interaction databases
STRINGi | 279010.BL00394 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 5 – 264 | 53EXOcInterPro annotationAdd BLAST | 260 | |
Domaini | 303 – 469 | 3'-5' exonucleaseInterPro annotationAdd BLAST | 167 | |
Domaini | 636 – 843 | POLAcInterPro annotationAdd BLAST | 208 |
Coiled coil
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Coiled coili | 500 – 520 | Sequence analysisAdd BLAST | 21 |
Sequence similaritiesi
Keywords - Domaini
Coiled coilSequence analysisPhylogenomic databases
eggNOGi | COG0258, Bacteria COG0749, Bacteria |
HOGENOMi | CLU_004675_0_0_9 |
OMAi | NRPPMPD |
OrthoDBi | 1220182at2 |
Family and domain databases
CDDi | cd09898, H3TH_53EXO, 1 hit |
Gene3Di | 3.30.420.10, 1 hit |
InterProi | View protein in InterPro IPR002562, 3'-5'_exonuclease_dom IPR020046, 5-3_exonucl_a-hlix_arch_N IPR002421, 5-3_exonuclease IPR036279, 5-3_exonuclease_C_sf IPR019760, DNA-dir_DNA_pol_A_CS IPR001098, DNA-dir_DNA_pol_A_palm_dom IPR043502, DNA/RNA_pol_sf IPR020045, DNA_polI_H3TH IPR018320, DNA_polymerase_1 IPR002298, DNA_polymerase_A IPR008918, HhH2 IPR029060, PIN-like_dom_sf IPR012337, RNaseH-like_sf IPR036397, RNaseH_sf |
PANTHERi | PTHR10133, PTHR10133, 1 hit |
Pfami | View protein in Pfam PF01367, 5_3_exonuc, 1 hit PF02739, 5_3_exonuc_N, 1 hit PF00476, DNA_pol_A, 1 hit |
PRINTSi | PR00868, DNAPOLI |
SMARTi | View protein in SMART SM00474, 35EXOc, 1 hit SM00475, 53EXOc, 1 hit SM00279, HhH2, 1 hit SM00482, POLAc, 1 hit |
SUPFAMi | SSF47807, SSF47807, 1 hit SSF53098, SSF53098, 1 hit SSF56672, SSF56672, 1 hit SSF88723, SSF88723, 1 hit |
TIGRFAMsi | TIGR00593, pola, 1 hit |
PROSITEi | View protein in PROSITE PS00447, DNA_POLYMERASE_A, 1 hit |
i Sequence
Sequence statusi: Complete.
Q65G92-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MTERKKLVLV DGNSLAYRAF FALPLLSNEK GIHTNAVYGF TTILMKMLEE
60 70 80 90 100
EKPTHMLVAF DAGKTTFRHK TFKEYKGGRQ KTPPELSEQL PFIRELLDAY
110 120 130 140 150
RISRYELENY EADDIIGTLA KSAEKDGFEV KIFSGDKDLT QLATEGTTVA
160 170 180 190 200
ITKKGITDVE YYTPEHVREK YGLTPEQIID MKGLMGDSSD NIPGVPGVGE
210 220 230 240 250
KTAIKLLKQF HTVEELLSSI DEVSGKKLKE KLEEFKEQAL MSKELATITT
260 270 280 290 300
EAPLEVSLDS LGYEGFDREA VVKIFKDLGF HSLLERIGEE AGEKEEEQLE
310 320 330 340 350
EIDVMIKTDI TDDLFASPAS LVVEQLGDNY HEAPILGFSI VNEHGAFFIP
360 370 380 390 400
EETAVQSDCF KEWAEDESKK KWVFDAKRAA VALRWRGIEL KGAEFDVLLA
410 420 430 440 450
AYIINPGHSY DDVASVAKEH QLHIVSADEA VYGKGAKQAV PDEKELADHL
460 470 480 490 500
ARKAKAISLL REKLLDELEE NEQLELFEAL EMPLAHILGE MESIGVQVDV
510 520 530 540 550
DRLKKMGEEL SAKLAEYEKK IHESAGETFN INSPKQLGVI LFDKLGLPVV
560 570 580 590 600
KKTKTGYSTS ADVLEKLRDK HVIIEDILHY RQIGKLQSTY VEGLLKVIKK
610 620 630 640 650
DSHKVHTRFN QALTQTGRLS STDPNLQNIP IRLEEGRKIR QAFVPSQKGW
660 670 680 690 700
LIFAADYSQI ELRVLAHISK DKNLIEAFTN DMDVHTKTAM DVFHVSEEEV
710 720 730 740 750
TPAMRRQAKA VNFGIVYGIS DYGLSQNLGI TRKEAAAFIE RYFHSFQGVK
760 770 780 790 800
EYMEETVQEA KQRGYVTTLL SRRRYIPELT SRNFNLRSFA ERTAMNTPIQ
810 820 830 840 850
GSAADIIKKA MIDMADKLKE KNLQAKLLLQ VHDELIFEAP EDEIKVLEKL
860 870
VPEVMEHALE LDVPLKVDCA SGPSWYDAK
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | CP000002 Genomic DNA Translation: AAU24563.1 |
RefSeqi | WP_011198188.1, NC_006322.1 |
Genome annotation databases
EnsemblBacteriai | AAU24563; AAU24563; BL00394 |
KEGGi | bli:BL00394 |
PATRICi | fig|279010.13.peg.3120 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | CP000002 Genomic DNA Translation: AAU24563.1 |
RefSeqi | WP_011198188.1, NC_006322.1 |
3D structure databases
SMRi | Q65G92 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 279010.BL00394 |
Genome annotation databases
EnsemblBacteriai | AAU24563; AAU24563; BL00394 |
KEGGi | bli:BL00394 |
PATRICi | fig|279010.13.peg.3120 |
Phylogenomic databases
eggNOGi | COG0258, Bacteria COG0749, Bacteria |
HOGENOMi | CLU_004675_0_0_9 |
OMAi | NRPPMPD |
OrthoDBi | 1220182at2 |
Enzyme and pathway databases
BioCyci | BLIC279010:BLI_RS15135-MONOMER |
Family and domain databases
CDDi | cd09898, H3TH_53EXO, 1 hit |
Gene3Di | 3.30.420.10, 1 hit |
InterProi | View protein in InterPro IPR002562, 3'-5'_exonuclease_dom IPR020046, 5-3_exonucl_a-hlix_arch_N IPR002421, 5-3_exonuclease IPR036279, 5-3_exonuclease_C_sf IPR019760, DNA-dir_DNA_pol_A_CS IPR001098, DNA-dir_DNA_pol_A_palm_dom IPR043502, DNA/RNA_pol_sf IPR020045, DNA_polI_H3TH IPR018320, DNA_polymerase_1 IPR002298, DNA_polymerase_A IPR008918, HhH2 IPR029060, PIN-like_dom_sf IPR012337, RNaseH-like_sf IPR036397, RNaseH_sf |
PANTHERi | PTHR10133, PTHR10133, 1 hit |
Pfami | View protein in Pfam PF01367, 5_3_exonuc, 1 hit PF02739, 5_3_exonuc_N, 1 hit PF00476, DNA_pol_A, 1 hit |
PRINTSi | PR00868, DNAPOLI |
SMARTi | View protein in SMART SM00474, 35EXOc, 1 hit SM00475, 53EXOc, 1 hit SM00279, HhH2, 1 hit SM00482, POLAc, 1 hit |
SUPFAMi | SSF47807, SSF47807, 1 hit SSF53098, SSF53098, 1 hit SSF56672, SSF56672, 1 hit SSF88723, SSF88723, 1 hit |
TIGRFAMsi | TIGR00593, pola, 1 hit |
PROSITEi | View protein in PROSITE PS00447, DNA_POLYMERASE_A, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | Q65G92_BACLD | |
Accessioni | Q65G92Primary (citable) accession number: Q65G92 Secondary accession number(s): Q62RP7 | |
Entry historyi | Integrated into UniProtKB/TrEMBL: | October 25, 2004 |
Last sequence update: | October 25, 2004 | |
Last modified: | May 25, 2022 | |
This is version 146 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Unreviewed (UniProtKB/TrEMBL) |