UniProtKB - Q65CJ7 (HPPR_PLESU)
Protein
Hydroxyphenylpyruvate reductase
Gene
HPPR
Organism
Plectranthus scutellarioides (Coleus) (Solenostemon scutellarioides)
Status
Functioni
Catalyzes the NAD(P)H-dependent reduction of 4-hydroxyphenylpyruvate to 4-hydroxyphenyllactate and 3,4-dihydroxyphenylpyruvate to 3,4-dihydroxyphenyllactate in the biosynthesis of rosmarinic acid. Rosmarinic acid is an ester of caffeic acid and 3,4-dihydroxyphenyllactic acid. NADP is the preferred substrate.1 Publication
Catalytic activityi
- (2R)-2-hydroxy-3-(4-hydroxyphenyl)propanoate + NAD+ = 3-(4-hydroxyphenyl)pyruvate + H+ + NADH1 PublicationEC:1.1.1.2371 Publication
- (2R)-2-hydroxy-3-(4-hydroxyphenyl)propanoate + NADP+ = 3-(4-hydroxyphenyl)pyruvate + H+ + NADPH1 PublicationEC:1.1.1.2371 Publication
- (2R)-3-(3,4-dihydroxyphenyl)lactate + NADP+ = 3-(3,4-dihydroxyphenyl)pyruvate + H+ + NADPH1 PublicationEC:1.1.1.2371 Publication
- (2R)-3-(3,4-dihydroxyphenyl)lactate + NAD+ = 3-(3,4-dihydroxyphenyl)pyruvate + H+ + NADH1 PublicationEC:1.1.1.2371 Publication
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 230 | NADP; via carbonyl oxygen1 Publication | 1 | |
Active sitei | 232 | By similarity | 1 | |
Binding sitei | 256 | NADP1 Publication | 1 | |
Active sitei | 261 | By similarity | 1 | |
Active sitei | 279 | Proton donorBy similarity | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 152 – 155 | NADP1 Publication | 4 | |
Nucleotide bindingi | 174 – 176 | NADP1 Publication | 3 |
GO - Molecular functioni
- hydroxyphenylpyruvate reductase activity Source: UniProtKB
- NAD binding Source: InterPro
- R(+)-3,4-dihydroxyphenyllactate:NADP+ oxidoreductase activity Source: UniProtKB-EC
GO - Biological processi
- oxidation-reduction process Source: UniProtKB
Keywordsi
Molecular function | Oxidoreductase |
Ligand | NAD, NADP, Nucleotide-binding, Pyruvate |
Enzyme and pathway databases
BioCyci | MetaCyc:MONOMER-11763 |
BRENDAi | 1.1.1.237, 1561 |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:HPPR |
Organismi | Plectranthus scutellarioides (Coleus) (Solenostemon scutellarioides) |
Taxonomic identifieri | 4142 [NCBI] |
Taxonomic lineagei | Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliopsida › eudicotyledons › Gunneridae › Pentapetalae › asterids › lamiids › Lamiales › Lamiaceae › Nepetoideae › Ocimeae › Plectranthus |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000422581 | 1 – 313 | Hydroxyphenylpyruvate reductaseAdd BLAST | 313 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | Q65CJ7 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | Q65CJ7 |
Family & Domainsi
Sequence similaritiesi
Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family.Curated
Family and domain databases
InterProi | View protein in InterPro IPR006139, D-isomer_2_OHA_DH_cat_dom IPR029752, D-isomer_DH_CS1 IPR006140, D-isomer_DH_NAD-bd IPR036291, NAD(P)-bd_dom_sf |
Pfami | View protein in Pfam PF00389, 2-Hacid_dh, 1 hit PF02826, 2-Hacid_dh_C, 1 hit |
SUPFAMi | SSF51735, SSF51735, 1 hit |
PROSITEi | View protein in PROSITE PS00065, D_2_HYDROXYACID_DH_1, 1 hit |
i Sequence
Sequence statusi: Complete.
Q65CJ7-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MEAIGVLMMC PMSTYLEQEL DKRFKLFRYW TQPAQRDFLA LQAESIRAVV
60 70 80 90 100
GNSNAGADAE LIDALPKLEI VSSFSVGLDK VDLIKCEEKG VRVTNTPDVL
110 120 130 140 150
TDDVADLAIG LILAVLRRIC ECDKYVRRGA WKFGDFKLTT KFSGKRVGII
160 170 180 190 200
GLGRIGLAVA ERAEAFDCPI SYFSRSKKPN TNYTYYGSVV ELASNSDILV
210 220 230 240 250
VACPLTPETT HIINREVIDA LGPKGVLINI GRGPHVDEPE LVSALVEGRL
260 270 280 290 300
GGAGLDVFER EPEVPEKLFG LENVVLLPHV GSGTVETRKV MADLVVGNLE
310
AHFSGKPLLT PVV
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AJ507733 mRNA Translation: CAD47810.2 |
Genome annotation databases
KEGGi | ag:CAD47810 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AJ507733 mRNA Translation: CAD47810.2 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
3BA1 | X-ray | 1.47 | A | 1-313 | [»] | |
3BAZ | X-ray | 2.20 | A | 1-313 | [»] | |
SMRi | Q65CJ7 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Genome annotation databases
KEGGi | ag:CAD47810 |
Enzyme and pathway databases
BioCyci | MetaCyc:MONOMER-11763 |
BRENDAi | 1.1.1.237, 1561 |
Miscellaneous databases
EvolutionaryTracei | Q65CJ7 |
Family and domain databases
InterProi | View protein in InterPro IPR006139, D-isomer_2_OHA_DH_cat_dom IPR029752, D-isomer_DH_CS1 IPR006140, D-isomer_DH_NAD-bd IPR036291, NAD(P)-bd_dom_sf |
Pfami | View protein in Pfam PF00389, 2-Hacid_dh, 1 hit PF02826, 2-Hacid_dh_C, 1 hit |
SUPFAMi | SSF51735, SSF51735, 1 hit |
PROSITEi | View protein in PROSITE PS00065, D_2_HYDROXYACID_DH_1, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | HPPR_PLESU | |
Accessioni | Q65CJ7Primary (citable) accession number: Q65CJ7 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | May 29, 2013 |
Last sequence update: | May 24, 2005 | |
Last modified: | December 2, 2020 | |
This is version 78 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Plant Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencingDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families