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Protein

All-trans-retinol 13,14-reductase

Gene

Retsat

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the saturation of all-trans-retinol to all-trans-13,14-dihydroretinol (PubMed:15358783, PubMed:17253779, PubMed:19139408). Does not exhibit any activity toward all-trans-retinoic acid, nor 9-cis, 11-cis or 13-cis-retinol isomers (PubMed:15358783). May play a role in the metabolism of vitamin A (PubMed:15358783, PubMed:17253779). Independently of retinol conversion, may regulate liver metabolism upstream of MLXIPL/ChREBP (PubMed:28855500). Required for adipocyte differentiation in a 3T3-L1 cell culture model (PubMed:19139408). This effect seems not to mimic the in vivo situation in which animals show increased adiposity in the absence of RETSAT (PubMed:19940255).1 Publication5 Publications

Catalytic activityi

All-trans-13,14-dihydroretinol + acceptor = all-trans-retinol + reduced acceptor.3 Publications

Cofactori

NAD+By similarity, NADP+By similarity, FADBy similarity

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi69 – 97FAD or NAD or NADPSequence analysisAdd BLAST29

GO - Molecular functioni

  • all-trans-retinol 13,14-reductase activity Source: HGNC
  • oxidoreductase activity Source: HGNC

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
LigandFAD, Flavoprotein, NAD, NADP

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-16797
BRENDAi1.3.99.23 3474

Names & Taxonomyi

Protein namesi
Recommended name:
All-trans-retinol 13,14-reductase (EC:1.3.99.233 Publications)
Alternative name(s):
All-trans-13,14-dihydroretinol saturase
Short name:
RetSat
PPAR-alpha-regulated and starvation-induced gene protein
Gene namesi
Name:Retsat
Synonyms:Ppsig
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 6

Organism-specific databases

MGIiMGI:1914692 Retsat

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

Pathology & Biotechi

Disruption phenotypei

Knockout mice fed either low-fat or high-fat (HFD) diets gain weight at a similar rate as their wild-type littermates and show normal insulin resistance and glucose tolerance. However, they exhibit increased adiposity and increased expression of key adipogenic markers, including PPAR-gamma and its target adipocyte P2 (aP2/FABP4), while maintained on an HFD.1 Publication

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi1 – 17Missing : Loss of localization to the endoplasmic reticulum. 1 PublicationAdd BLAST17
Mutagenesisi91 – 96Missing : Loss of catalytic activity, as measured by the saturation of retinol to 13,14-dihydroretinol. Loss of adipocyte differentiation in a 3T3-L1 cell culture model. 1 Publication6
Mutagenesisi96E → A: Loss of catalytic activity, as measured by the saturation of retinol to 13,14-dihydroretinol. Loss of adipocyte differentiation in a 3T3-L1 cell culture model. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 25Sequence analysisAdd BLAST25
ChainiPRO_000022566726 – 609All-trans-retinol 13,14-reductaseAdd BLAST584

Proteomic databases

MaxQBiQ64FW2
PaxDbiQ64FW2
PRIDEiQ64FW2

PTM databases

iPTMnetiQ64FW2
PhosphoSitePlusiQ64FW2

Expressioni

Tissue specificityi

Predominantly expressed in the liver (at protein level) (PubMed:19940255). Also expressed at high levels in kidney, intestine, and white fat and brown fat (PubMed:15358783, PubMed:18289917, PubMed:19139408, PubMed:28855500). Weakly expressed in heart, skeletal muscle and testis and barely detected in the lung, brain and spleen (PubMed:15358783, PubMed:18289917, PubMed:28855500). Up-regulated in the liver of diet-induced obese mice, compared to lean animals (PubMed:28855500). Down-regulated in adipose tissue of obese mice; this decrease could be due to the impact of inflammatory cells on adipocytes (PubMed:19139408).5 Publications

Developmental stagei

Up-regulated during adipocyte differentiation in a 3T3-L1 cell culture model (at protein level).2 Publications

Inductioni

Up-regulated during starvation (PubMed:18289917). Up-regulated by PPARG (PubMed:19139408).2 Publications

Gene expression databases

BgeeiENSMUSG00000056666 Expressed in 238 organ(s), highest expression level in liver
ExpressionAtlasiQ64FW2 baseline and differential
GenevisibleiQ64FW2 MM

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000068568

Structurei

3D structure databases

ProteinModelPortaliQ64FW2
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG4254 Eukaryota
COG1233 LUCA
GeneTreeiENSGT00390000017613
HOGENOMiHOG000233930
HOVERGENiHBG079484
InParanoidiQ64FW2
KOiK09516
OMAiTPANEPW
OrthoDBiEOG091G04VP
TreeFamiTF328375

Family and domain databases

Gene3Di3.50.50.60, 2 hits
InterProiView protein in InterPro
IPR036188 FAD/NAD-bd_sf
SUPFAMiSSF51905 SSF51905, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry has 1 described isoform and 4 potential isoforms that are computationally mapped.Show allAlign All

Q64FW2-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MWITALLLAV LLLVILHRVY VGLYAASSPN PFAEDVKRPP EPLVTDKEAR
60 70 80 90 100
KKVLKQAFSV SRVPEKLDAV VIGSGIGGLA SAAVLAKAGK RVLVLEQHTK
110 120 130 140 150
AGGCCHTFGE NGLEFDTGIH YIGRMREGNI GRFILDQITE GQLDWAPMAS
160 170 180 190 200
PFDLMILEGP NGRKEFPMYS GRKEYIQGLK KKFPKEEAVI DKYMELVKVV
210 220 230 240 250
ARGVSHAVLL KFLPLPLTQL LSKFGLLTRF SPFCRASTQS LAEVLQQLGA
260 270 280 290 300
SRELQAVLSY IFPTYGVTPS HTAFSLHALL VDHYIQGAYY PRGGSSEIAF
310 320 330 340 350
HTIPLIQRAG GAVLTRATVQ SVLLDSAGRA CGVSVKKGQE LVNIYCPVVI
360 370 380 390 400
SNAGMFNTYQ HLLPETVRHL PDVKKQLAMV RPGLSMLSIF ICLKGTKEDL
410 420 430 440 450
KLQSTNYYVY FDTDMDKAME RYVSMPKEKA PEHIPLLFIA FPSSKDPTWE
460 470 480 490 500
ERFPDRSTMT ALVPMAFEWF EEWQEEPKGK RGVDYETLKN AFVEASMSVI
510 520 530 540 550
MKLFPQLEGK VESVTGGSPL TNQYYLAAPR GATYGADHDL ARLHPHAMAS
560 570 580 590 600
IRAQTPIPNL YLTGQDIFTC GLMGALQGAL LCSSAILKRN LYSDLQALGS

KVKAQKKKM
Length:609
Mass (Da):67,334
Last modified:July 27, 2011 - v3
Checksum:i7BC21825B8A4ABD2
GO

Computationally mapped potential isoform sequencesi

There are 4 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
H3BKK1H3BKK1_MOUSE
All-trans-retinol 13,14-reductase
Retsat
400Annotation score:
H3BJ51H3BJ51_MOUSE
All-trans-retinol 13,14-reductase
Retsat
548Annotation score:
F7CW61F7CW61_MOUSE
All-trans-retinol 13,14-reductase
Retsat
99Annotation score:
A0A0U1RPP6A0A0U1RPP6_MOUSE
All-trans-retinol 13,14-reductase
Retsat
147Annotation score:

Sequence cautioni

The sequence BAB22406 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti273A → T in AAL73986 (PubMed:11753649).Curated1
Sequence conflicti273A → T in BAE25708 (PubMed:16141072).Curated1
Sequence conflicti293G → R in AAL73986 (PubMed:11753649).Curated1
Sequence conflicti293G → R in BAE25708 (PubMed:16141072).Curated1
Sequence conflicti306I → V in AAH11203 (PubMed:15489334).Curated1
Sequence conflicti451E → D in BAE25708 (PubMed:16141072).Curated1
Sequence conflicti495A → V in BAB22406 (PubMed:16141072).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF466400 Genomic DNA Translation: AAL73986.1
AY704159 mRNA Translation: AAU25836.1
EF620363 mRNA Translation: ABS17600.1
EF620364 mRNA Translation: ABS17601.1
EF620365 mRNA Translation: ABS17602.1
EF620366 Genomic DNA Translation: ABS17603.1
AK002851 mRNA Translation: BAB22406.2 Different initiation.
AK144115 mRNA Translation: BAE25708.1
AC125039 Genomic DNA No translation available.
CH466523 Genomic DNA Translation: EDK98989.1
BC011203 mRNA Translation: AAH11203.2
BC117751 mRNA Translation: AAI17752.1
CCDSiCCDS20244.1
RefSeqiNP_080435.3, NM_026159.4
UniGeneiMm.305108

Genome annotation databases

EnsembliENSMUST00000070597; ENSMUSP00000068568; ENSMUSG00000056666
GeneIDi67442
KEGGimmu:67442
UCSCiuc009cix.1 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF466400 Genomic DNA Translation: AAL73986.1
AY704159 mRNA Translation: AAU25836.1
EF620363 mRNA Translation: ABS17600.1
EF620364 mRNA Translation: ABS17601.1
EF620365 mRNA Translation: ABS17602.1
EF620366 Genomic DNA Translation: ABS17603.1
AK002851 mRNA Translation: BAB22406.2 Different initiation.
AK144115 mRNA Translation: BAE25708.1
AC125039 Genomic DNA No translation available.
CH466523 Genomic DNA Translation: EDK98989.1
BC011203 mRNA Translation: AAH11203.2
BC117751 mRNA Translation: AAI17752.1
CCDSiCCDS20244.1
RefSeqiNP_080435.3, NM_026159.4
UniGeneiMm.305108

3D structure databases

ProteinModelPortaliQ64FW2
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000068568

PTM databases

iPTMnetiQ64FW2
PhosphoSitePlusiQ64FW2

Proteomic databases

MaxQBiQ64FW2
PaxDbiQ64FW2
PRIDEiQ64FW2

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000070597; ENSMUSP00000068568; ENSMUSG00000056666
GeneIDi67442
KEGGimmu:67442
UCSCiuc009cix.1 mouse

Organism-specific databases

CTDi54884
MGIiMGI:1914692 Retsat

Phylogenomic databases

eggNOGiKOG4254 Eukaryota
COG1233 LUCA
GeneTreeiENSGT00390000017613
HOGENOMiHOG000233930
HOVERGENiHBG079484
InParanoidiQ64FW2
KOiK09516
OMAiTPANEPW
OrthoDBiEOG091G04VP
TreeFamiTF328375

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-16797
BRENDAi1.3.99.23 3474

Miscellaneous databases

ChiTaRSiRetsat mouse
PROiPR:Q64FW2
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000056666 Expressed in 238 organ(s), highest expression level in liver
ExpressionAtlasiQ64FW2 baseline and differential
GenevisibleiQ64FW2 MM

Family and domain databases

Gene3Di3.50.50.60, 2 hits
InterProiView protein in InterPro
IPR036188 FAD/NAD-bd_sf
SUPFAMiSSF51905 SSF51905, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiRETST_MOUSE
AccessioniPrimary (citable) accession number: Q64FW2
Secondary accession number(s): Q149J8
, Q3UNN9, Q78JX8, Q8VHE7, Q9CW85
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 7, 2006
Last sequence update: July 27, 2011
Last modified: November 7, 2018
This is version 116 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
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Main funding by: National Institutes of Health

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