UniProtKB - Q64962 (RDRP_ASPVP)
Protein
RNA replication polyprotein
Gene
ORF1
Organism
Apple stem pitting virus (isolate PA66) (ASPV)
Status
Functioni
RNA-directed RNA polymerase involved in viral RNA replication.By similarity
Protease: Thiol protease that cleaves the polyprotein.By similarity
Catalytic activityi
Cofactori
Fe2+PROSITE-ProRule annotationNote: Binds 1 Fe2+ ion per subunit.PROSITE-ProRule annotation
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 780 | IronPROSITE-ProRule annotation | 1 | |
Metal bindingi | 782 | IronPROSITE-ProRule annotation | 1 | |
Metal bindingi | 835 | IronPROSITE-ProRule annotation | 1 | |
Binding sitei | 844 | 2-oxoglutaratePROSITE-ProRule annotation | 1 | |
Active sitei | 1202 | By similarity | 1 | |
Active sitei | 1283 | By similarity | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 1374 – 1381 | ATPPROSITE-ProRule annotation | 8 |
GO - Molecular functioni
- ATP binding Source: UniProtKB-KW
- cysteine-type peptidase activity Source: UniProtKB-KW
- dioxygenase activity Source: UniProtKB-KW
- metal ion binding Source: UniProtKB-KW
- mRNA methyltransferase activity Source: InterPro
- RNA binding Source: InterPro
- RNA-directed 5'-3' RNA polymerase activity Source: UniProtKB-KW
- RNA helicase activity Source: UniProtKB-EC
GO - Biological processi
- RNA processing Source: InterPro
- transcription, DNA-templated Source: InterPro
- viral RNA genome replication Source: InterPro
Keywordsi
Molecular function | Dioxygenase, Helicase, Hydrolase, Methyltransferase, Multifunctional enzyme, Nucleotidyltransferase, Oxidoreductase, Protease, RNA-directed RNA polymerase, Thiol protease, Transferase |
Biological process | Viral RNA replication |
Ligand | ATP-binding, Iron, Metal-binding, Nucleotide-binding |
Protein family/group databases
MEROPSi | C23.001 |
Names & Taxonomyi
Protein namesi | Recommended name: RNA replication polyproteinBy similarityAlternative name(s): ORF1 proteinBy similarity Cleaved into the following chain: Including the following 4 domains: Putative Fe(2+) 2-oxoglutarate dioxygenaseSequence analysis (EC:1.14.11.-PROSITE-ProRule annotation) |
Gene namesi | ORF Names:ORF1By similarity |
Organismi | Apple stem pitting virus (isolate PA66) (ASPV) |
Taxonomic identifieri | 651356 [NCBI] |
Taxonomic lineagei | Viruses › Riboviria › Orthornavirae › Kitrinoviricota › Alsuviricetes › Tymovirales › Betaflexiviridae › Quinvirinae › Foveavirus › |
Virus hosti | Crataegus (hawthorn) [TaxID: 23159] Malus sieboldii [TaxID: 106566] Malus sylvestris [TaxID: 3752] Pyrus communis (Pear) (Pyrus domestica) [TaxID: 23211] |
Proteomesi |
|
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000401087 | 1 – 2183 | RNA replication polyproteinAdd BLAST | 2183 | |
ChainiPRO_0000431909 | 1686 – 2177 | HelicaseBy similarityAdd BLAST | 492 |
Post-translational modificationi
Specific enzymatic cleavages by the viral protease yield mature proteins.By similarity
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sitei | 1685 – 1686 | Cleavage; by viral proteaseBy similarity | 2 |
Proteomic databases
PRIDEi | Q64962 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 63 – 256 | Alphavirus-like MTPROSITE-ProRule annotationAdd BLAST | 194 | |
Domaini | 762 – 853 | Fe2OG dioxygenasePROSITE-ProRule annotationAdd BLAST | 92 | |
Domaini | 1091 – 1199 | OTUPROSITE-ProRule annotationAdd BLAST | 109 | |
Domaini | 1198 – 1288 | Peptidase C23PROSITE-ProRule annotationAdd BLAST | 91 | |
Domaini | 1349 – 1520 | (+)RNA virus helicase ATP-bindingPROSITE-ProRule annotationAdd BLAST | 172 | |
Domaini | 1521 – 1667 | (+)RNA virus helicase C-terminalPROSITE-ProRule annotationAdd BLAST | 147 | |
Domaini | 1961 – 2068 | RdRp catalyticPROSITE-ProRule annotationAdd BLAST | 108 |
Sequence similaritiesi
Belongs to the potexviruses/carlaviruses RNA replication protein family.Curated
Family and domain databases
Gene3Di | 2.60.120.590, 1 hit |
InterProi | View protein in InterPro IPR027351, (+)RNA_virus_helicase_core_dom IPR037151, AlkB-like_sf IPR002588, Alphavirus-like_MT_dom IPR043502, DNA/RNA_pol_sf IPR003323, OTU_dom IPR005123, Oxoglu/Fe-dep_dioxygenase IPR027417, P-loop_NTPase IPR008041, Peptidase_C23 IPR007094, RNA-dir_pol_PSvirus IPR001788, Tymovirus_RNA-dep_RNA_pol |
Pfami | View protein in Pfam PF03171, 2OG-FeII_Oxy, 1 hit PF05379, Peptidase_C23, 1 hit PF00978, RdRP_2, 1 hit PF01443, Viral_helicase1, 1 hit PF01660, Vmethyltransf, 1 hit |
SUPFAMi | SSF52540, SSF52540, 1 hit SSF56672, SSF56672, 1 hit |
PROSITEi | View protein in PROSITE PS51743, ALPHAVIRUS_MT, 1 hit PS51471, FE2OG_OXY, 1 hit PS50802, OTU, 1 hit PS51492, PEPTIDASE_C23, 1 hit PS51657, PSRV_HELICASE, 1 hit PS50507, RDRP_SSRNA_POS, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
Q64962-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MALLSRTAAE EVIASFTSEE QSRISTQAVL ALTNVEKDKH DLFNYALPEL
60 70 80 90 100
AKMRLFNSGI YLSPHSYRPH SHPVCKTLEN NILFNILPSY LDNSFYLVSI
110 120 130 140 150
KKNKVDFLKR RHPDLQMVET INRYISSIDK TRYGGFFHVS PSKISAKFKC
160 170 180 190 200
DRRTGFEDDA SLIDLIPGCM EGARKRFFFH DELHYWTKEA LITFLDHVKP
210 220 230 240 250
EVMLASIVFP PEILAGAKES LNPWCYTFRI VGKDLVFFPD GEQSEAYIQP
260 270 280 290 300
VAGSYLLRTG KITTPSGDIF QLDLLKSSFS HHLISITKGE AIGQKMRFFN
310 320 330 340 350
GFEAVAMKGL NPLRRKVESC LPISKNTILK IYRYLRTLKK PDLQSAMAKL
360 370 380 390 400
SQVCKDPNGY EIKFFEEFSK LCLKCDTLNT NMIPDMKRIV QGFFLKLFPN
410 420 430 440 450
PISRNFKVVQ QLHLDNFIET LEEFNFSINT ESLSLNWKDD LEFVNLTFGD
460 470 480 490 500
TDFNVEDSFA EAWGTKKDVV NITTVHHSPY LVSKFESYDH QFHSILSVKS
510 520 530 540 550
ISALTRIAKI VLSLYDPCVV EAFSESRVTN LAVNVIIAAN LRACFAVTDL
560 570 580 590 600
WRIFEGILLK ECKRAQGKMR KRFHFELGIR WFLFVDVSNQ WFLPPCRDGL
610 620 630 640 650
IARSVSFDQF IKGCQRDNSL HNGRMSLRQV LKGPKLQALF DVSELSIIHN
660 670 680 690 700
VEMENAPEAG STLDAGIKPT SSPLEVVPIE NARCNLAPCK CDLNCFIQPA
710 720 730 740 750
DVNSLHGNLV FLDFIGGSKG RGASFYSRDL KGYSYTGFSH VSRGWPAFLD
760 770 780 790 800
KFLSDNKIPL NFYNQCLVQE YSTGHGLSMH KDDESIYDIN HQVLTVNYSG
810 820 830 840 850
DAIFCIECLG SGFEIPLSGP QMLLMPFGFQ KEHRHGIKSP SKGRISLTFR
860 870 880 890 900
LTKEGDSQVP IQEVVTICDH GDSDDRAALK ALERRSHQSG GRPAVELEGH
910 920 930 940 950
EREKVNSDSS DSAPVQEFLI QIDSSLLEYA LKSLSGLSKN VVNCDMCLCN
960 970 980 990 1000
SPWLKNEELR FSEALRDLAF AQGLIQLIDF LCLKVLRCAE VNRIISELPT
1010 1020 1030 1040 1050
HVFPLRGTMH IVDLDDESIR GDVKEGSFSG FRRWKVMSCS TDLIMLAFLK
1060 1070 1080 1090 1100
PKMTLGGELR SHEDECELSD LTEKLHGCSV ILSRKFEPDL FHSFDVEADG
1110 1120 1130 1140 1150
NCFWHSVGPL IGVDGEYLKR ILHDQAKKDG VKCPRLSKQL EGNTWAEREA
1160 1170 1180 1190 1200
VAYFCSHYGI RLNVLYTREE CTWIFKPHEV LKAATLICQD NHFKPCMPVN
1210 1220 1230 1240 1250
GCVIRAISSA LNRREVDVLA VLGKPAHEDL FEEVAEGRGF SIFDLTRLFE
1260 1270 1280 1290 1300
IFSICGSVDT GGELIMVNEN GRIPAEFSLE KEHLAHIPTL SRRKFSPIVS
1310 1320 1330 1340 1350
DLNRVSNSAM RFLAINGAEV DYRPSIDRAS TLLDSFEIGA TGVLCQGIKE
1360 1370 1380 1390 1400
AQKDLASKLI PELVHERKLI MILGTFGCGK SSLFKKFIEK SPGKAITFVS
1410 1420 1430 1440 1450
PRRSLAESIN HDLGLARVGG KKTGKSKDLK NVRVKTFELF ILHLDSIKEG
1460 1470 1480 1490 1500
HTVVIDEIQL FPPGYIDLII LGLKPNVNII IAGDPCQSDY DCSSDRHIFA
1510 1520 1530 1540 1550
GSESDIMRIL SGRSYKFNIL SQRFRNPVFY GRLPCNLNKT RLTLDEEEYT
1560 1570 1580 1590 1600
LWDSIQEFSM MGRKDCPVVL VSSFEEKKIV AAHLGLKMKC ITYGESTGLN
1610 1620 1630 1640 1650
FQKGAILVTY ESALTSDRRW WTALSRFSHD IHFINGMGVT WDNAITHFVG
1660 1670 1680 1690 1700
KPLHKFFTKR ACNDDIIDLL PGRPELIEGF QSQVGADEGV REAKLVGDPW
1710 1720 1730 1740 1750
LKTKIFLGQN PDFEIEIADE VEAAEDWFKT HIPIMSLEAV RAQWVHKLIS
1760 1770 1780 1790 1800
REDREFRIGD ITTEQFTDDH SKNRGQELTN AAERYEAIYP RHKGTDTATF
1810 1820 1830 1840 1850
LMAVKKRLSF SSPAAEHAKL RRAKPFGKFL LDTFLKRVPL NSSHDEKMMQ
1860 1870 1880 1890 1900
EAVHAFEEKK LSKSMATIEN HSGRSCEDWP VDKALIFMKS QLCTKFDNRF
1910 1920 1930 1940 1950
RSAKAGQTLA CFQHSVLCRF APYMRYIESK VTEVLPKNLY IHSGKNIDDL
1960 1970 1980 1990 2000
AAWVTTSKFN GVCTESDYEA FDASQDHFIL AFELEVMKFL GLPSDLIADY
2010 2020 2030 2040 2050
TFIKTHLGSK LGSFAIMRFT GEASTFLFNT MANMLFTFLR YDLNGREAIC
2060 2070 2080 2090 2100
FAGDDMCANS RLKVTNRFSN FLDKIKLKAK VQFTATPTFC GWGLCEHGVF
2110 2120 2130 2140 2150
KKPDLVLERL QIARETRNLE NCIDNYAIEV SCAYKMGENL NLYLTPQEVD
2160 2170 2180
AHYNCVRFIV QHNHLLKSNI RDLFKGESLP ASS
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | D21829 Genomic RNA Translation: BAA04853.1 |
RefSeqi | NP_604464.1, NC_003462.2 |
Genome annotation databases
GeneIDi | 935270 |
KEGGi | vg:935270 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | D21829 Genomic RNA Translation: BAA04853.1 |
RefSeqi | NP_604464.1, NC_003462.2 |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein family/group databases
MEROPSi | C23.001 |
Proteomic databases
PRIDEi | Q64962 |
Genome annotation databases
GeneIDi | 935270 |
KEGGi | vg:935270 |
Family and domain databases
Gene3Di | 2.60.120.590, 1 hit |
InterProi | View protein in InterPro IPR027351, (+)RNA_virus_helicase_core_dom IPR037151, AlkB-like_sf IPR002588, Alphavirus-like_MT_dom IPR043502, DNA/RNA_pol_sf IPR003323, OTU_dom IPR005123, Oxoglu/Fe-dep_dioxygenase IPR027417, P-loop_NTPase IPR008041, Peptidase_C23 IPR007094, RNA-dir_pol_PSvirus IPR001788, Tymovirus_RNA-dep_RNA_pol |
Pfami | View protein in Pfam PF03171, 2OG-FeII_Oxy, 1 hit PF05379, Peptidase_C23, 1 hit PF00978, RdRP_2, 1 hit PF01443, Viral_helicase1, 1 hit PF01660, Vmethyltransf, 1 hit |
SUPFAMi | SSF52540, SSF52540, 1 hit SSF56672, SSF56672, 1 hit |
PROSITEi | View protein in PROSITE PS51743, ALPHAVIRUS_MT, 1 hit PS51471, FE2OG_OXY, 1 hit PS50802, OTU, 1 hit PS51492, PEPTIDASE_C23, 1 hit PS51657, PSRV_HELICASE, 1 hit PS50507, RDRP_SSRNA_POS, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | RDRP_ASPVP | |
Accessioni | Q64962Primary (citable) accession number: Q64962 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 2, 2010 |
Last sequence update: | November 1, 1996 | |
Last modified: | October 7, 2020 | |
This is version 105 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Viral Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families