UniProtKB - Q64727 (VINC_MOUSE)
Vinculin
Vcl
Functioni
Actin filament (F-actin)-binding protein involved in cell-matrix adhesion and cell-cell adhesion. Regulates cell-surface E-cadherin expression and potentiates mechanosensing by the E-cadherin complex. May also play important roles in cell morphology and locomotion (By similarity).
By similarity1 PublicationGO - Molecular functioni
- actin filament binding Source: InterPro
- alpha-catenin binding Source: MGI
- dystroglycan binding Source: MGI
- small GTPase binding Source: MGI
- structural molecule activity Source: InterPro
- ubiquitin protein ligase binding Source: MGI
GO - Biological processi
- adherens junction assembly Source: MGI
- apical junction assembly Source: MGI
- axon extension Source: MGI
- cell adhesion Source: MGI
- epithelial cell-cell adhesion Source: MGI
- lamellipodium assembly Source: MGI
- morphogenesis of an epithelium Source: MGI
- protein localization to cell surface Source: MGI
- regulation of cell migration Source: MGI
- regulation of establishment of endothelial barrier Source: MGI
- regulation of focal adhesion assembly Source: MGI
- regulation of protein localization to adherens junction Source: MGI
Keywordsi
Molecular function | Actin-binding |
Biological process | Cell adhesion |
Enzyme and pathway databases
Reactomei | R-MMU-114608, Platelet degranulation R-MMU-445355, Smooth Muscle Contraction R-MMU-5674135, MAP2K and MAPK activation R-MMU-6798695, Neutrophil degranulation |
Names & Taxonomyi
Protein namesi | Recommended name: VinculinAlternative name(s): Metavinculin |
Gene namesi | Name:Vcl |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:98927, Vcl |
VEuPathDBi | HostDB:ENSMUSG00000021823 |
Subcellular locationi
Cytoskeleton
- cytoskeleton By similarity
Plasma membrane
- Cell membrane By similarity; Peripheral membrane protein By similarity; Cytoplasmic side By similarity
- sarcolemma 1 Publication; Peripheral membrane protein 1 Publication; Cytoplasmic side 1 Publication
Other locations
- adherens junction By similarity
- focal adhesion By similarity
Note: Recruitment to cell-cell junctions occurs in a myosin II-dependent manner. Interaction with CTNNB1 is necessary for its localization to the cell-cell junctions.By similarity
Cytoskeleton
- actin cytoskeleton Source: MGI
- podosome Source: MGI
- terminal web Source: AgBase
Plasma Membrane
- plasma membrane Source: MGI
- sarcolemma Source: UniProtKB
Other locations
- adherens junction Source: MGI
- brush border Source: AgBase
- cell-cell contact zone Source: MGI
- cell-cell junction Source: MGI
- costamere Source: MGI
- cytoplasm Source: UniProtKB
- fascia adherens Source: MGI
- focal adhesion Source: UniProtKB
- inner dense plaque of desmosome Source: AgBase
- intercalated disc Source: MGI
- membrane raft Source: MGI
- outer dense plaque of desmosome Source: AgBase
- protein-containing complex Source: MGI
- Z disc Source: MGI
- zonula adherens Source: AgBase
Keywords - Cellular componenti
Cell junction, Cell membrane, Cytoplasm, Cytoskeleton, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000064253 | 1 – 1066 | VinculinAdd BLAST | 1066 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 97 | PhosphoserineBy similarity | 1 | |
Modified residuei | 173 | N6-acetyllysineBy similarity | 1 | |
Modified residuei | 260 | PhosphoserineCombined sources | 1 | |
Modified residuei | 272 | PhosphoserineBy similarity | 1 | |
Modified residuei | 275 | PhosphoserineBy similarity | 1 | |
Modified residuei | 290 | PhosphoserineCombined sources | 1 | |
Modified residuei | 346 | PhosphoserineCombined sources | 1 | |
Modified residuei | 434 | PhosphoserineBy similarity | 1 | |
Modified residuei | 496 | N6-acetyllysineBy similarity | 1 | |
Modified residuei | 537 | PhosphotyrosineCurated | 1 | |
Modified residuei | 574 | PhosphoserineBy similarity | 1 | |
Modified residuei | 579 | PhosphoserineCombined sources | 1 | |
Modified residuei | 600 | PhosphoserineCombined sources | 1 | |
Modified residuei | 604 | PhosphothreonineBy similarity | 1 | |
Modified residuei | 672 | PhosphothreonineBy similarity | 1 | |
Modified residuei | 721 | PhosphoserineCombined sources | 1 | |
Modified residuei | 795 | PhosphoserineBy similarity | 1 | |
Modified residuei | 809 | PhosphoserineCombined sources | 1 | |
Modified residuei | 822 | PhosphotyrosineCombined sources | 1 | |
Modified residuei | 1065 | Phosphotyrosine; by SRC-type Tyr-kinasesBy similarity | 1 |
Post-translational modificationi
Keywords - PTMi
Acetylation, Lipoprotein, Palmitate, PhosphoproteinProteomic databases
CPTACi | non-CPTAC-3755 |
EPDi | Q64727 |
jPOSTi | Q64727 |
MaxQBi | Q64727 |
PaxDbi | Q64727 |
PeptideAtlasi | Q64727 |
PRIDEi | Q64727 |
ProteomicsDBi | 298282 |
2D gel databases
REPRODUCTION-2DPAGEi | Q64727 |
PTM databases
iPTMneti | Q64727 |
PhosphoSitePlusi | Q64727 |
Expressioni
Gene expression databases
Bgeei | ENSMUSG00000021823, Expressed in urinary bladder and 312 other tissues |
ExpressionAtlasi | Q64727, baseline and differential |
Genevisiblei | Q64727, MM |
Interactioni
Subunit structurei
Exhibits self-association properties.
Part of a complex composed of THSD1, PTK2/FAK1, TLN1 and VCL (By similarity).
Interacts with APBB1IP, NRAP and TLN1.
Interacts with SYNM.
Interacts with CTNNB1 and this interaction is necessary for its localization to the cell-cell junctions and for its function in regulating cell surface expression of E-cadherin (By similarity).
Interacts with SORBS1 (PubMed:10085297).
Interacts with SYNM (By similarity).
Interacts with CTNNA1 (By similarity). Binds to ACTN4; this interaction triggers conformational changes (By similarity).
By similarity2 PublicationsBinary interactionsi
Q64727
GO - Molecular functioni
- actin filament binding Source: InterPro
- alpha-catenin binding Source: MGI
- dystroglycan binding Source: MGI
- small GTPase binding Source: MGI
- ubiquitin protein ligase binding Source: MGI
Protein-protein interaction databases
BioGRIDi | 204506, 44 interactors |
CORUMi | Q64727 |
IntActi | Q64727, 16 interactors |
MINTi | Q64727 |
STRINGi | 10090.ENSMUSP00000022369 |
Miscellaneous databases
RNActi | Q64727, protein |
Structurei
Secondary structure
3D structure databases
BMRBi | Q64727 |
SMRi | Q64727 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Repeati | 259 – 369 | 1Sequence analysisAdd BLAST | 111 | |
Repeati | 370 – 479 | 2Sequence analysisAdd BLAST | 110 | |
Repeati | 480 – 589 | 3Sequence analysisAdd BLAST | 110 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 835 | N-terminal globular headBy similarityAdd BLAST | 835 | |
Regioni | 168 – 208 | Talin-interactionBy similarityAdd BLAST | 41 | |
Regioni | 259 – 589 | 3 X 112 AA tandem repeatsSequence analysisAdd BLAST | 331 | |
Regioni | 741 – 764 | Interaction with ACTN4By similarityAdd BLAST | 24 | |
Regioni | 836 – 878 | Linker (Pro-rich)By similarityAdd BLAST | 43 | |
Regioni | 857 – 887 | DisorderedSequence analysisAdd BLAST | 31 | |
Regioni | 879 – 1066 | C-terminal tailBy similarityAdd BLAST | 188 | |
Regioni | 935 – 978 | Facilitates phospholipid membrane insertion1 PublicationAdd BLAST | 44 | |
Regioni | 1052 – 1066 | Facilitates phospholipid membrane insertion1 PublicationAdd BLAST | 15 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 859 – 873 | Pro residuesSequence analysisAdd BLAST | 15 |
Domaini
Sequence similaritiesi
Keywords - Domaini
RepeatPhylogenomic databases
eggNOGi | KOG3681, Eukaryota |
GeneTreei | ENSGT01030000234543 |
HOGENOMi | CLU_012338_0_0_1 |
InParanoidi | Q64727 |
OMAi | MVNEPMM |
OrthoDBi | 270073at2759 |
PhylomeDBi | Q64727 |
TreeFami | TF313686 |
Family and domain databases
InterProi | View protein in InterPro IPR036723, Alpha-catenin/vinculin-like_sf IPR017997, Vinculin IPR006077, Vinculin/catenin IPR000633, Vinculin_CS |
PANTHERi | PTHR46180, PTHR46180, 1 hit |
Pfami | View protein in Pfam PF01044, Vinculin, 2 hits |
SUPFAMi | SSF47220, SSF47220, 6 hits |
PROSITEi | View protein in PROSITE PS00663, VINCULIN_1, 1 hit PS00664, VINCULIN_2, 3 hits |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
10 20 30 40 50
MPVFHTRTIE SILEPVAQQI SHLVIMHEEG EVDGKAIPDL TAPVAAVQAA
60 70 80 90 100
VSNLVRVGKE TVQTTEDQIL KRDMPPAFIK VENACTKLVQ AAQMLQSDPY
110 120 130 140 150
SVPARDYLID GSRGILSGTS DLLLTFDEAE VRKIIRVCKG ILEYLTVAEV
160 170 180 190 200
VETMEDLVTY TKNLGPGMTK MAKMIDERQQ ELTHQEHRVM LVNSMNTVKE
210 220 230 240 250
LLPVLISAMK IFVTTKNSKN QGIEEALKNR NFTVEKMSAE INEIIRVLQL
260 270 280 290 300
TSWDEDAWAS KDTEAMKRAL ASIDSKLNQA KGWLRDPNAS PGDAGEQAIR
310 320 330 340 350
QILDEAGKVG ELCAGKERRE ILGTCKMLGQ MTDQVADLRA RGQGASPVAM
360 370 380 390 400
QKAQQVSQGL DVLTAKVENA ARKLEAMTNS KQSIAKKIDA AQNWLADPNG
410 420 430 440 450
GPEGEEQIRG ALAEARKIAE LCDDPKERDD ILRSLGEIAA LTSKLGDLRR
460 470 480 490 500
QGKGDSPEAR ALAKQVATAL QNLQTKTNRA VANSRPAKAA VHLEGKIEQA
510 520 530 540 550
QRWIDNPTVD DRGVGQAAIR GLVAEGHRLA NVMMGPYRQD LLAKCDRVDQ
560 570 580 590 600
LTAQLADLAA RGEGESPQAR ALASQLQDSL KDLKAQMQEA MTQEVSDVFS
610 620 630 640 650
DTTTPIKLLA VAATAPPDAP NREEVFDERA ANFENHSGRL GATAEKAAAV
660 670 680 690 700
GTANKSTVEG IQASVKTARE LTPQVISAAR ILLRNPGNQA AYEHFETMKN
710 720 730 740 750
QWIDNVEKMT GLVDEAIDTK SLLDASEEAI KKDLDKCKVA MANIQPQMLV
760 770 780 790 800
AGATSIARRA NRILLVAKRE VENSEDPKFR EAVKAASDEL SKTISPMVMD
810 820 830 840 850
AKAVAGNISD PGLQKSFLDS GYRILGAVAK VREAFQPQEP DFPPPPPDLE
860 870 880 890 900
QLRLTDELAP PKPPLPEGEV PPPRPPPPEE KDEEFPEQKA GEVINQPMMM
910 920 930 940 950
AARQLHDEAR KWSSKGNDII AAAKRMALLM AEMSRLVRGG SGTKRALIQC
960 970 980 990 1000
AKDIAKASDE VTRLAKEVAK QCTDKRIRTN LLQVCERIPT ISTQLKILST
1010 1020 1030 1040 1050
VKATMLGRTN ISDEESEQAT EMLVHNAQNL MQSVKETVRE AEAASIKIRT
1060
DAGFTLRWVR KTPWYQ
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketA0A286YDJ4 | A0A286YDJ4_MOUSE | Metavinculin | Vcl | 149 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 16 | V → E in AAB96843 (PubMed:7568093).Curated | 1 | |
Sequence conflicti | 215 | T → S in AAB96843 (PubMed:7568093).Curated | 1 | |
Sequence conflicti | 363 | L → V in AAB96843 (PubMed:7568093).Curated | 1 | |
Sequence conflicti | 499 | Q → K in BAC37033 (PubMed:16141072).Curated | 1 | |
Sequence conflicti | 501 | Q → R in AAB96843 (PubMed:7568093).Curated | 1 | |
Sequence conflicti | 799 | M → K in BAC37033 (PubMed:16141072).Curated | 1 | |
Sequence conflicti | 812 | G → D in AAB96843 (PubMed:7568093).Curated | 1 | |
Sequence conflicti | 1044 | A → T in BAC37033 (PubMed:16141072).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | L13300, L13299 Genomic DNA Translation: AAA40557.1 L18880 mRNA Translation: AAB96843.1 AK035184 mRNA Translation: BAC28973.1 AK077850 mRNA Translation: BAC37033.1 BC008520 mRNA Translation: AAH08520.1 BC008554 mRNA Translation: AAH08554.1 |
CCDSi | CCDS26860.1 |
PIRi | A60965 T10108 |
RefSeqi | NP_033528.3, NM_009502.4 |
Genome annotation databases
Ensembli | ENSMUST00000022369; ENSMUSP00000022369; ENSMUSG00000021823 |
GeneIDi | 22330 |
KEGGi | mmu:22330 |
UCSCi | uc007skz.1, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | L13300, L13299 Genomic DNA Translation: AAA40557.1 L18880 mRNA Translation: AAB96843.1 AK035184 mRNA Translation: BAC28973.1 AK077850 mRNA Translation: BAC37033.1 BC008520 mRNA Translation: AAH08520.1 BC008554 mRNA Translation: AAH08554.1 |
CCDSi | CCDS26860.1 |
PIRi | A60965 T10108 |
RefSeqi | NP_033528.3, NM_009502.4 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
5Y04 | X-ray | 2.85 | A | 1-250 | [»] | |
BMRBi | Q64727 | |||||
SMRi | Q64727 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 204506, 44 interactors |
CORUMi | Q64727 |
IntActi | Q64727, 16 interactors |
MINTi | Q64727 |
STRINGi | 10090.ENSMUSP00000022369 |
PTM databases
iPTMneti | Q64727 |
PhosphoSitePlusi | Q64727 |
2D gel databases
REPRODUCTION-2DPAGEi | Q64727 |
Proteomic databases
CPTACi | non-CPTAC-3755 |
EPDi | Q64727 |
jPOSTi | Q64727 |
MaxQBi | Q64727 |
PaxDbi | Q64727 |
PeptideAtlasi | Q64727 |
PRIDEi | Q64727 |
ProteomicsDBi | 298282 |
Protocols and materials databases
Antibodypediai | 884, 911 antibodies from 45 providers |
DNASUi | 22330 |
Genome annotation databases
Ensembli | ENSMUST00000022369; ENSMUSP00000022369; ENSMUSG00000021823 |
GeneIDi | 22330 |
KEGGi | mmu:22330 |
UCSCi | uc007skz.1, mouse |
Organism-specific databases
CTDi | 7414 |
MGIi | MGI:98927, Vcl |
VEuPathDBi | HostDB:ENSMUSG00000021823 |
Phylogenomic databases
eggNOGi | KOG3681, Eukaryota |
GeneTreei | ENSGT01030000234543 |
HOGENOMi | CLU_012338_0_0_1 |
InParanoidi | Q64727 |
OMAi | MVNEPMM |
OrthoDBi | 270073at2759 |
PhylomeDBi | Q64727 |
TreeFami | TF313686 |
Enzyme and pathway databases
Reactomei | R-MMU-114608, Platelet degranulation R-MMU-445355, Smooth Muscle Contraction R-MMU-5674135, MAP2K and MAPK activation R-MMU-6798695, Neutrophil degranulation |
Miscellaneous databases
BioGRID-ORCSi | 22330, 2 hits in 63 CRISPR screens |
ChiTaRSi | Vcl, mouse |
PROi | PR:Q64727 |
RNActi | Q64727, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000021823, Expressed in urinary bladder and 312 other tissues |
ExpressionAtlasi | Q64727, baseline and differential |
Genevisiblei | Q64727, MM |
Family and domain databases
InterProi | View protein in InterPro IPR036723, Alpha-catenin/vinculin-like_sf IPR017997, Vinculin IPR006077, Vinculin/catenin IPR000633, Vinculin_CS |
PANTHERi | PTHR46180, PTHR46180, 1 hit |
Pfami | View protein in Pfam PF01044, Vinculin, 2 hits |
SUPFAMi | SSF47220, SSF47220, 6 hits |
PROSITEi | View protein in PROSITE PS00663, VINCULIN_1, 1 hit PS00664, VINCULIN_2, 3 hits |
MobiDBi | Search... |
Entry informationi
Entry namei | VINC_MOUSE | |
Accessioni | Q64727Primary (citable) accession number: Q64727 Secondary accession number(s): Q8BP32 Q922D9 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 15, 1998 |
Last sequence update: | January 23, 2007 | |
Last modified: | February 23, 2022 | |
This is version 187 of the entry and version 4 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families