UniProtKB - Q64705 (USF2_MOUSE)
Upstream stimulatory factor 2
Usf2
Functioni
Transcription factor that binds to a symmetrical DNA sequence (E-boxes) (5'-CACGTG-3') that is found in a variety of viral and cellular promoters.
GO - Molecular functioni
- bHLH transcription factor binding Source: MGI
- DNA binding Source: MGI
- DNA-binding transcription factor activity Source: MGI
- DNA-binding transcription factor activity, RNA polymerase II-specific Source: MGI
- double-stranded DNA binding Source: MGI
- identical protein binding Source: MGI
- protein-containing complex binding Source: MGI
- protein heterodimerization activity Source: MGI
- protein homodimerization activity Source: MGI
- RNA polymerase II cis-regulatory region sequence-specific DNA binding Source: MGI
- sequence-specific DNA binding Source: MGI
- sequence-specific double-stranded DNA binding Source: MGI
GO - Biological processi
- lactation Source: MGI
- lipid homeostasis Source: BHF-UCL
- positive regulation of transcription, DNA-templated Source: MGI
- positive regulation of transcription by RNA polymerase II Source: MGI
- positive regulation of transcription from RNA polymerase II promoter by glucose Source: BHF-UCL
- regulation of transcription by RNA polymerase II Source: MGI
Keywordsi
Molecular function | DNA-binding |
Biological process | Transcription, Transcription regulation |
Enzyme and pathway databases
Reactomei | R-MMU-9018519, Estrogen-dependent gene expression |
Names & Taxonomyi
Protein namesi | Recommended name: Upstream stimulatory factor 2Alternative name(s): Major late transcription factor 2 Upstream transcription factor 2 |
Gene namesi | Name:Usf2 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:99961, Usf2 |
VEuPathDBi | HostDB:ENSMUSG00000058239 |
Subcellular locationi
Nucleus
Nucleus
- nucleoplasm Source: MGI
- nucleus Source: MGI
Other locations
- intracellular membrane-bounded organelle Source: MGI
Keywords - Cellular componenti
NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000127501 | 1 – 346 | Upstream stimulatory factor 2Add BLAST | 346 |
Proteomic databases
EPDi | Q64705 |
MaxQBi | Q64705 |
PaxDbi | Q64705 |
PRIDEi | Q64705 |
ProteomicsDBi | 299646 [Q64705-1] 299647 [Q64705-2] |
PTM databases
iPTMneti | Q64705 |
PhosphoSitePlusi | Q64705 |
Expressioni
Gene expression databases
Bgeei | ENSMUSG00000058239, Expressed in retinal neural layer and 297 other tissues |
Genevisiblei | Q64705, MM |
Interactioni
Subunit structurei
Efficient DNA binding requires dimerization with another bHLH protein. Binds DNA as a homodimer or a heterodimer (USF1/USF2).
Interacts with MAF.
1 PublicationBinary interactionsi
Q64705
With | #Exp. | IntAct |
---|---|---|
Stat1 [P42225] | 3 | EBI-647583,EBI-647118 |
GO - Molecular functioni
- bHLH transcription factor binding Source: MGI
- identical protein binding Source: MGI
- protein heterodimerization activity Source: MGI
- protein homodimerization activity Source: MGI
Protein-protein interaction databases
ComplexPortali | CPX-3084, USF2 upstream stimulatory factor complex CPX-3086, USF1-USF2 upstream stimulatory factor complex |
IntActi | Q64705, 2 interactors |
STRINGi | 10090.ENSMUSP00000132256 |
Miscellaneous databases
RNActi | Q64705, protein |
Structurei
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 235 – 290 | bHLHPROSITE-ProRule annotationAdd BLAST | 56 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 44 | DisorderedSequence analysisAdd BLAST | 44 | |
Regioni | 215 – 244 | DisorderedSequence analysisAdd BLAST | 30 | |
Regioni | 307 – 328 | Leucine-zipperAdd BLAST | 22 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 226 – 244 | Basic and acidic residuesSequence analysisAdd BLAST | 19 |
Phylogenomic databases
eggNOGi | KOG1318, Eukaryota |
GeneTreei | ENSGT00940000160704 |
InParanoidi | Q64705 |
OMAi | CAQDHTK |
OrthoDBi | 1345445at2759 |
PhylomeDBi | Q64705 |
TreeFami | TF323338 |
Family and domain databases
Gene3Di | 4.10.280.10, 1 hit |
InterProi | View protein in InterPro IPR011598, bHLH_dom IPR036638, HLH_DNA-bd_sf |
Pfami | View protein in Pfam PF00010, HLH, 1 hit |
SMARTi | View protein in SMART SM00353, HLH, 1 hit |
SUPFAMi | SSF47459, SSF47459, 1 hit |
PROSITEi | View protein in PROSITE PS50888, BHLH, 1 hit |
s (2+)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 2 described isoforms and 5 potential isoforms that are computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MDMLDPGLDP ASSATAAAAA SHDKGPEAEE GVELQEGGDG PGAEEQTAVA
60 70 80 90 100
IASVQQAAFG DHNIQYQFRT ESNGGQVTYR VVQVTDGQLD GQGDAAGAVS
110 120 130 140 150
VVSTAAFAGG QQAVTQVGVD GAAQRPGPAA ASVPTGPAAP FPLAVIQNPF
160 170 180 190 200
SNGGSPAAEA VSGEARFAYF PASSVGDTTA VSVQTTDQSL QAGGQFYVMM
210 220 230 240 250
TPQDVLQTGT QRTIAPRTHP YSPKIDGTRT PRDERRRAQH NEVERRRRDK
260 270 280 290 300
INNWIVQLSK IIPDCHADNS KTGASKGGIL SKACDYIREL RQTNQRMQET
310 320 330 340
FKEAERLQMD NELLRQQIEE LKNENALLRA QLQQHNLEMV GESTRQ
The sequence of this isoform differs from the canonical sequence as follows:
77-143: Missing.
Computationally mapped potential isoform sequencesi
There are 5 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketF6PWU6 | F6PWU6_MOUSE | Upstream stimulatory factor 2 | Usf2 | 293 | Annotation score: | ||
E9PXC2 | E9PXC2_MOUSE | Upstream stimulatory factor 2 | Usf2 | 215 | Annotation score: | ||
F8WI12 | F8WI12_MOUSE | Upstream stimulatory factor 2 | Usf2 | 319 | Annotation score: | ||
F6WLF9 | F6WLF9_MOUSE | Upstream stimulatory factor 2 | Usf2 | 38 | Annotation score: | ||
E9PZY3 | E9PZY3_MOUSE | Upstream stimulatory factor 2 | Usf2 | 47 | Annotation score: |
Sequence cautioni
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 240 – 243 | Missing in CAA54697 (PubMed:7774954).Curated | 4 | |
Sequence conflicti | 326 | A → S in CAA54697 (PubMed:7774954).Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_002166 | 77 – 143 | Missing in isoform USF2B. 1 PublicationAdd BLAST | 67 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U12283, U12282 Genomic DNA Translation: AAB60674.1 U01662 mRNA Translation: AAA20492.1 U01663 mRNA Translation: AAA20493.1 Different initiation. X77602, X77605 Genomic DNA Translation: CAA54697.1 BC019729 mRNA Translation: AAH19729.1 Different initiation. BC082995 mRNA Translation: AAH82995.1 AK146871 mRNA Translation: BAE27494.1 |
CCDSi | CCDS21118.1 [Q64705-1] |
PIRi | A55111 |
RefSeqi | NP_035810.1, NM_011680.2 [Q64705-1] XP_006539796.1, XM_006539733.3 [Q64705-2] XP_006539797.1, XM_006539734.3 XP_006539798.1, XM_006539735.3 |
Genome annotation databases
Ensembli | ENSMUST00000058860; ENSMUSP00000132256; ENSMUSG00000058239 [Q64705-1] ENSMUST00000108119; ENSMUSP00000132021; ENSMUSG00000058239 [Q64705-2] |
GeneIDi | 22282 |
KEGGi | mmu:22282 |
UCSCi | uc009ghg.2, mouse [Q64705-2] uc009ghh.2, mouse [Q64705-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U12283, U12282 Genomic DNA Translation: AAB60674.1 U01662 mRNA Translation: AAA20492.1 U01663 mRNA Translation: AAA20493.1 Different initiation. X77602, X77605 Genomic DNA Translation: CAA54697.1 BC019729 mRNA Translation: AAH19729.1 Different initiation. BC082995 mRNA Translation: AAH82995.1 AK146871 mRNA Translation: BAE27494.1 |
CCDSi | CCDS21118.1 [Q64705-1] |
PIRi | A55111 |
RefSeqi | NP_035810.1, NM_011680.2 [Q64705-1] XP_006539796.1, XM_006539733.3 [Q64705-2] XP_006539797.1, XM_006539734.3 XP_006539798.1, XM_006539735.3 |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
ComplexPortali | CPX-3084, USF2 upstream stimulatory factor complex CPX-3086, USF1-USF2 upstream stimulatory factor complex |
IntActi | Q64705, 2 interactors |
STRINGi | 10090.ENSMUSP00000132256 |
PTM databases
iPTMneti | Q64705 |
PhosphoSitePlusi | Q64705 |
Proteomic databases
EPDi | Q64705 |
MaxQBi | Q64705 |
PaxDbi | Q64705 |
PRIDEi | Q64705 |
ProteomicsDBi | 299646 [Q64705-1] 299647 [Q64705-2] |
Protocols and materials databases
Antibodypediai | 15859, 378 antibodies from 34 providers |
DNASUi | 22282 |
Genome annotation databases
Ensembli | ENSMUST00000058860; ENSMUSP00000132256; ENSMUSG00000058239 [Q64705-1] ENSMUST00000108119; ENSMUSP00000132021; ENSMUSG00000058239 [Q64705-2] |
GeneIDi | 22282 |
KEGGi | mmu:22282 |
UCSCi | uc009ghg.2, mouse [Q64705-2] uc009ghh.2, mouse [Q64705-1] |
Organism-specific databases
CTDi | 7392 |
MGIi | MGI:99961, Usf2 |
VEuPathDBi | HostDB:ENSMUSG00000058239 |
Phylogenomic databases
eggNOGi | KOG1318, Eukaryota |
GeneTreei | ENSGT00940000160704 |
InParanoidi | Q64705 |
OMAi | CAQDHTK |
OrthoDBi | 1345445at2759 |
PhylomeDBi | Q64705 |
TreeFami | TF323338 |
Enzyme and pathway databases
Reactomei | R-MMU-9018519, Estrogen-dependent gene expression |
Miscellaneous databases
BioGRID-ORCSi | 22282, 0 hits in 65 CRISPR screens |
ChiTaRSi | Usf2, mouse |
PROi | PR:Q64705 |
RNActi | Q64705, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000058239, Expressed in retinal neural layer and 297 other tissues |
Genevisiblei | Q64705, MM |
Family and domain databases
Gene3Di | 4.10.280.10, 1 hit |
InterProi | View protein in InterPro IPR011598, bHLH_dom IPR036638, HLH_DNA-bd_sf |
Pfami | View protein in Pfam PF00010, HLH, 1 hit |
SMARTi | View protein in SMART SM00353, HLH, 1 hit |
SUPFAMi | SSF47459, SSF47459, 1 hit |
PROSITEi | View protein in PROSITE PS50888, BHLH, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | USF2_MOUSE | |
Accessioni | Q64705Primary (citable) accession number: Q64705 Secondary accession number(s): Q3UIL2, Q8VE59 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 1, 1997 |
Last sequence update: | November 1, 1996 | |
Last modified: | February 23, 2022 | |
This is version 164 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot