UniProtKB - Q64705 (USF2_MOUSE)

BLAST

>sp|Q64705|USF2_MOUSE Upstream stimulatory factor 2 OS=Mus musculus OX=10090 GN=Usf2 PE=1 SV=1
MDMLDPGLDPASSATAAAAASHDKGPEAEEGVELQEGGDGPGAEEQTAVAIASVQQAAFG
DHNIQYQFRTESNGGQVTYRVVQVTDGQLDGQGDAAGAVSVVSTAAFAGGQQAVTQVGVD
GAAQRPGPAAASVPTGPAAPFPLAVIQNPFSNGGSPAAEAVSGEARFAYFPASSVGDTTA
VSVQTTDQSLQAGGQFYVMMTPQDVLQTGTQRTIAPRTHPYSPKIDGTRTPRDERRRAQH
NEVERRRRDKINNWIVQLSKIIPDCHADNSKTGASKGGILSKACDYIRELRQTNQRMQET
FKEAERLQMDNELLRQQIEELKNENALLRAQLQQHNLEMVGESTRQ

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History
Entry version 147 (07 Nov 2018)
Sequence version 1 (01 Nov 1996)
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Protein

Upstream stimulatory factor 2

Gene

Usf2

Organism

Mus musculus (Mouse)

Status

Reviewed-Annotation score: -Experimental evidence at protein levelⁱ

Functionⁱ

Transcription factor that binds to a symmetrical DNA sequence (E-boxes) (5'-CACGTG-3') that is found in a variety of viral and cellular promoters.

GO - Molecular functionⁱ

bHLH transcription factor binding Source: MGI
DNA binding
Source: MGI
Inferred from direct assayⁱ
- 7523363
DNA-binding transcription factor activity Source: MGI
double-stranded DNA binding Source: MGI
protein heterodimerization activity Source: MGI
protein homodimerization activity Source: MGI
RNA polymerase II proximal promoter sequence-specific DNA binding Source: MGI
sequence-specific DNA binding Source: MGI
transcription factor activity, RNA polymerase II distal enhancer sequence-specific binding Source: MGI

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

lactation
Source: MGI
Inferred from mutant phenotypeⁱ
- 12907752
lipid homeostasis
Source: BHF-UCL
Inferred from mutant phenotypeⁱ
- 9890958
positive regulation of transcription, DNA-templated
Source: MGI
Inferred from direct assayⁱ
- 15466854
positive regulation of transcription by RNA polymerase II Source: MGI
positive regulation of transcription from RNA polymerase II promoter by glucose
Source: BHF-UCL
Inferred from mutant phenotypeⁱ
- 9890958
regulation of transcription by RNA polymerase II
Source: MGI
Inferred from direct assayⁱ
- 7523363
transcription by RNA polymerase II Source: MGI

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Molecular function	DNA-binding
Biological process	Transcription, Transcription regulation

Enzyme and pathway databases

Reactomeⁱ	R-MMU-9018519 Estrogen-dependent gene expression

Reactomeⁱ

R-MMU-9018519 Estrogen-dependent gene expression

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: Upstream stimulatory factor 2 Alternative name(s): Major late transcription factor 2 Upstream transcription factor 2
Gene namesⁱ	Name:Usf2
Organismⁱ	Mus musculus (Mouse)
Taxonomic identifierⁱ	10090 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Dipnotetrapodomorpha › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Boreoeutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus
Proteomesⁱ	UP000000589 Componentⁱ: Chromosome 7

Organism-specific databases

MGIⁱ	MGI:99961 Usf2

Keywords - Cellular componentⁱ

Nucleus

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
ChainⁱPRO_0000127501	1 – 346	Upstream stimulatory factor 2Add BLAST		346

Proteomic databases

EPDⁱ	Q64705
PaxDbⁱ	Q64705
PRIDEⁱ	Q64705

PTM databases

iPTMnetⁱ	Q64705
PhosphoSitePlusⁱ	Q64705

Expressionⁱ

Gene expression databases

Bgeeⁱ	ENSMUSG00000058239 Expressed in 272 organ(s), highest expression level in brain
CleanExⁱ	MM_USF2
ExpressionAtlasⁱ	Q64705 baseline and differential
Genevisibleⁱ	Q64705 MM

Interactionⁱ

Subunit structureⁱ

Efficient DNA binding requires dimerization with another bHLH protein. Binds DNA as a homodimer or a heterodimer (USF1/USF2). Interacts with MAF.1 Publication

Binary interactionsⁱ

With	Entry	#Exp.	IntAct	Notes
Stat1	P42225	3	EBI-647583,EBI-647118

With

Entry

#Exp.

IntAct

Notes

Stat1

P42225

EBI-647583,EBI-647118

GO - Molecular functionⁱ

bHLH transcription factor binding Source: MGI
protein heterodimerization activity Source: MGI
protein homodimerization activity Source: MGI

View the complete GO annotation on QuickGO ...

Protein-protein interaction databases

ComplexPortalⁱ	CPX-3084 USF2 upstream stimulatory factor complex CPX-3086 USF1-USF2 upstream stimulatory factor complex
IntActⁱ	Q64705, 2 interactors
STRINGⁱ	10090.ENSMUSP00000132256

Structureⁱ

3D structure databases

ProteinModelPortalⁱ	Q64705
SMRⁱ	Q64705
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Family & Domainsⁱ

Domains and Repeats

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Domainⁱ	235 – 290	bHLHPROSITE-ProRule annotationAdd BLAST		56

Region

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Regionⁱ	307 – 328	Leucine-zipperAdd BLAST		22

Compositional bias

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Compositional biasⁱ	14 – 20	Poly-Ala		7

Phylogenomic databases

eggNOGⁱ	ENOG410INSC Eukaryota ENOG4111DV1 LUCA
Ensembl GeneTree More...GeneTreeⁱ	ENSGT00390000004498
HOGENOMⁱ	HOG000294174
HOVERGENⁱ	HBG004346
InParanoidⁱ	Q64705
KEGG Orthology (KO) More...KOⁱ	K09106
Database of Orthologous Groups More...OrthoDBⁱ	EOG091G0RH5
PhylomeDBⁱ	Q64705
TreeFamⁱ	TF323338

Family and domain databases

Conserved Domains Database More...CDDⁱ	cd00083 HLH, 1 hit
Gene3Dⁱ	4.10.280.10, 1 hit
InterProⁱ	View protein in InterPro IPR011598 bHLH_dom IPR024097 bHLH_ZIP_TF IPR036638 HLH_DNA-bd_sf
PANTHERⁱ	PTHR12565 PTHR12565, 1 hit
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF00010 HLH, 1 hit
SMARTⁱ	View protein in SMART SM00353 HLH, 1 hit
SUPFAMⁱ	SSF47459 SSF47459, 1 hit
PROSITEⁱ	View protein in PROSITE PS50888 BHLH, 1 hit

Sequences (2+)ⁱ

Sequence statusⁱ: Complete.

This entry describes 2 isoformsⁱ produced by alternative splicing. Align Add to basket

Note: Additional isoforms seem to exist.

This entry has 2 described isoforms and 5 potential isoforms that are computationally mapped.Show all Align All

Isoform USF2A (identifier: Q64705-1) [UniParc]FASTA Add to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MDMLDPGLDP ASSATAAAAA SHDKGPEAEE GVELQEGGDG PGAEEQTAVA 
        60         70         80         90        100
IASVQQAAFG DHNIQYQFRT ESNGGQVTYR VVQVTDGQLD GQGDAAGAVS 
       110        120        130        140        150
VVSTAAFAGG QQAVTQVGVD GAAQRPGPAA ASVPTGPAAP FPLAVIQNPF 
       160        170        180        190        200
SNGGSPAAEA VSGEARFAYF PASSVGDTTA VSVQTTDQSL QAGGQFYVMM 
       210        220        230        240        250
TPQDVLQTGT QRTIAPRTHP YSPKIDGTRT PRDERRRAQH NEVERRRRDK 
       260        270        280        290        300
INNWIVQLSK IIPDCHADNS KTGASKGGIL SKACDYIREL RQTNQRMQET 
       310        320        330        340 
FKEAERLQMD NELLRQQIEE LKNENALLRA QLQQHNLEMV GESTRQ

Length:346

Mass (Da):36,954

Last modified:November 1, 1996 - v1

Checksum:ⁱE9D216BC25F9447B

Isoform USF2B (identifier: Q64705-2) [UniParc]FASTA Add to basket

The sequence of this isoform differs from the canonical sequence as follows:
77-143: Missing.

« Hide

        10         20         30         40         50
MDMLDPGLDP ASSATAAAAA SHDKGPEAEE GVELQEGGDG PGAEEQTAVA 
        60         70         80         90        100
IASVQQAAFG DHNIQYQFRT ESNGGQAVIQ NPFSNGGSPA AEAVSGEARF 
       110        120        130        140        150
AYFPASSVGD TTAVSVQTTD QSLQAGGQFY VMMTPQDVLQ TGTQRTIAPR 
       160        170        180        190        200
THPYSPKIDG TRTPRDERRR AQHNEVERRR RDKINNWIVQ LSKIIPDCHA 
       210        220        230        240        250
DNSKTGASKG GILSKACDYI RELRQTNQRM QETFKEAERL QMDNELLRQQ 
       260        270 
IEELKNENAL LRAQLQQHNL EMVGESTRQ

Show »

Length:279

Mass (Da):30,595

Checksum:ⁱD9474CE884D8A60B

Computationally mapped potential isoform sequencesⁱ

There are 5 potential isoforms mapped to this entry.BLAST Align Show all Add to basket

Entry	Entry name	Protein names	Gene names	Length	Annotation

F6PWU6	F6PWU6_MOUSE	Upstream stimulatory factor 2 Upstream stimulatory factor 2	Usf2	293	Annotation score:
E9PXC2	E9PXC2_MOUSE	Upstream stimulatory factor 2 Upstream stimulatory factor 2	Usf2	215	Annotation score:
F8WI12	F8WI12_MOUSE	Upstream stimulatory factor 2 Upstream stimulatory factor 2	Usf2	319	Annotation score:
E9PZY3	E9PZY3_MOUSE	Upstream stimulatory factor 2 Upstream stimulatory factor 2	Usf2	47	Annotation score:
F6WLF9	F6WLF9_MOUSE	Upstream stimulatory factor 2 Upstream stimulatory factor 2	Usf2	38	Annotation score:

Sequence cautionⁱ

The sequence AAA20493 differs from that shown. Reason: Erroneous initiation.Curated

The sequence AAH19729 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Sequence conflictⁱ	240 – 243	Missing in CAA54697 (PubMed:7774954).Curated		4
Sequence conflictⁱ	326	A → S in CAA54697 (PubMed:7774954).Curated		1

Alternative sequence

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Alternative sequenceⁱVSP_002166	77 – 143	Missing in isoform USF2B. 1 PublicationAdd BLAST		67

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	U12283, U12282 Genomic DNA Translation: AAB60674.1 U01662 mRNA Translation: AAA20492.1 U01663 mRNA Translation: AAA20493.1 Different initiation. X77602, X77605 Genomic DNA Translation: CAA54697.1 BC019729 mRNA Translation: AAH19729.1 Different initiation. BC082995 mRNA Translation: AAH82995.1 AK146871 mRNA Translation: BAE27494.1
The Consensus CDS (CCDS) project More...CCDSⁱ	CCDS21118.1 [Q64705-1]
PIRⁱ	A55111
NCBI Reference Sequences More...RefSeqⁱ	NP_035810.1, NM_011680.2 [Q64705-1] XP_006539796.1, XM_006539733.3 [Q64705-2] XP_006539797.1, XM_006539734.3 XP_006539798.1, XM_006539735.3
UniGeneⁱ	Mm.322453

Genome annotation databases

Ensemblⁱ	ENSMUST00000058860; ENSMUSP00000132256; ENSMUSG00000058239 [Q64705-1] ENSMUST00000108119; ENSMUSP00000132021; ENSMUSG00000058239 [Q64705-2]
GeneIDⁱ	22282
KEGGⁱ	mmu:22282
UCSC genome browser More...UCSCⁱ	uc009ghg.2 mouse [Q64705-2] uc009ghh.2 mouse [Q64705-1]

Keywords - Coding sequence diversityⁱ

Alternative splicing

Similar proteinsⁱ

100% Identity
90% Identity
50% Identity

Protein	Similar proteins		Species	Score	Length	Source
Q64705	Upstream stimulatory factor 2		RAT		346	UniRef100_Q64705

Protein	Similar proteins		Species	Score	Length	Source
Q64705	Upstream stimulatory factor 2		HUMAN		346	UniRef90_Q15853
Upstream stimulatory factor 2		RAT		346
USF2 isoform 2		PANTR		346
USF2 isoform 2		PONAB		346
Uncharacterized protein		CAVPO		360
+61
Q64705-2	Isoform USF2B of Upstream stimulatory factor 2		HUMAN		279	UniRef90_Q15853-3
USF2 isoform 1		PANTR		279
USF2 isoform 1		PONAB		279
Upstream transcription factor 2, c-fos interacting		GORGO		258
upstream stimulatory factor 2 isoform X2		LIPVE		280
+26

Protein	Similar proteins		Species	Score	Length	Source
Q64705	Upstream stimulatory factor 2		HUMAN		346	UniRef50_Q15853
Upstream stimulatory factor 2		RAT		346
USF2 isoform 2		PANTR		346
USF2 isoform 2		PONAB		346
Uncharacterized protein		CAVPO		360
+97
Q64705-2	Isoform USF2B of Upstream stimulatory factor 2		HUMAN		279	UniRef50_Q15853-3
USF2 isoform 1		PANTR		279
USF2 isoform 1		PONAB		279
Upstream transcription factor 2, c-fos interacting		GORGO		258
upstream stimulatory factor 2 isoform X2		LIPVE		280
+48

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	U12283, U12282 Genomic DNA Translation: AAB60674.1 U01662 mRNA Translation: AAA20492.1 U01663 mRNA Translation: AAA20493.1 Different initiation. X77602, X77605 Genomic DNA Translation: CAA54697.1 BC019729 mRNA Translation: AAH19729.1 Different initiation. BC082995 mRNA Translation: AAH82995.1 AK146871 mRNA Translation: BAE27494.1
CCDSⁱ	CCDS21118.1 [Q64705-1]
PIRⁱ	A55111
RefSeqⁱ	NP_035810.1, NM_011680.2 [Q64705-1] XP_006539796.1, XM_006539733.3 [Q64705-2] XP_006539797.1, XM_006539734.3 XP_006539798.1, XM_006539735.3
UniGeneⁱ	Mm.322453

3D structure databases

ProteinModelPortalⁱ	Q64705
SMRⁱ	Q64705
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

ComplexPortalⁱ	CPX-3084 USF2 upstream stimulatory factor complex CPX-3086 USF1-USF2 upstream stimulatory factor complex
IntActⁱ	Q64705, 2 interactors
STRINGⁱ	10090.ENSMUSP00000132256

PTM databases

iPTMnetⁱ	Q64705
PhosphoSitePlusⁱ	Q64705

Proteomic databases

EPDⁱ	Q64705
PaxDbⁱ	Q64705
PRIDEⁱ	Q64705

Protocols and materials databases

Structural Biology Knowledgebase	Search...

Structural Biology Knowledgebase

Search...

Genome annotation databases

Ensemblⁱ	ENSMUST00000058860; ENSMUSP00000132256; ENSMUSG00000058239 [Q64705-1] ENSMUST00000108119; ENSMUSP00000132021; ENSMUSG00000058239 [Q64705-2]
GeneIDⁱ	22282
KEGGⁱ	mmu:22282
UCSCⁱ	uc009ghg.2 mouse [Q64705-2] uc009ghh.2 mouse [Q64705-1]

Organism-specific databases

CTDⁱ	7392
MGIⁱ	MGI:99961 Usf2

Phylogenomic databases

eggNOGⁱ	ENOG410INSC Eukaryota ENOG4111DV1 LUCA
GeneTreeⁱ	ENSGT00390000004498
HOGENOMⁱ	HOG000294174
HOVERGENⁱ	HBG004346
InParanoidⁱ	Q64705
KOⁱ	K09106
OrthoDBⁱ	EOG091G0RH5
PhylomeDBⁱ	Q64705
TreeFamⁱ	TF323338

Enzyme and pathway databases

Reactomeⁱ	R-MMU-9018519 Estrogen-dependent gene expression

Reactomeⁱ

R-MMU-9018519 Estrogen-dependent gene expression

Miscellaneous databases

Protein Ontology More...PROⁱ	PR:Q64705
SOURCEⁱ	Search...

Gene expression databases

Bgeeⁱ	ENSMUSG00000058239 Expressed in 272 organ(s), highest expression level in brain
CleanExⁱ	MM_USF2
ExpressionAtlasⁱ	Q64705 baseline and differential
Genevisibleⁱ	Q64705 MM

Family and domain databases

CDDⁱ	cd00083 HLH, 1 hit
Gene3Dⁱ	4.10.280.10, 1 hit
InterProⁱ	View protein in InterPro IPR011598 bHLH_dom IPR024097 bHLH_ZIP_TF IPR036638 HLH_DNA-bd_sf
PANTHERⁱ	PTHR12565 PTHR12565, 1 hit
Pfamⁱ	View protein in Pfam PF00010 HLH, 1 hit
SMARTⁱ	View protein in SMART SM00353 HLH, 1 hit
SUPFAMⁱ	SSF47459 SSF47459, 1 hit
PROSITEⁱ	View protein in PROSITE PS50888 BHLH, 1 hit
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	USF2_MOUSE
Accessionⁱ	Q64705Primary (citable) accession number: Q64705 Secondary accession number(s): Q3UIL2, Q8VE59
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
	Last sequence update:	November 1, 1996
	Last modified:	November 7, 2018
	This is version 147 of the entry and version 1 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Chordata Protein Annotation Program

Miscellaneousⁱ

Keywords - Technical termⁱ

Complete proteome, Reference proteome

Documents

MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

UniProtKB

UniParc

Help

UniRef

Proteomes

Annotation systems

Supporting data

UniProtKB - Q64705 (USF2_MOUSE)

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Upstream stimulatory factor 2

Usf2

Select a section on the left to see content.

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

Enzyme and pathway databases

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

Organism-specific databases

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Nucleus

Nucleus

Other locations

Keywords - Cellular componenti

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Proteomic databases

PTM databases

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

Gene expression databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

GO - Molecular functioni

Protein-protein interaction databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

Region

Compositional bias

Phylogenomic databases

Family and domain databases

<p>This subsection of the ‘Sequence’ section reports difference(s) between the protein sequence shown in the UniProtKB entry and other available protein sequences derived from the same gene.<p><a href='/help/sequence_caution' target='_top'>More...</a></p>Sequence cautioni

Experimental Info

Alternative sequence

Sequence databases

Genome annotation databases

Keywords - Coding sequence diversityi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Miscellaneous databases

Gene expression databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

Documents

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Functionⁱ

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

Keywords - Cellular componentⁱ

PTM / Processingⁱ

Expressionⁱ

Interactionⁱ

GO - Molecular functionⁱ

Structureⁱ

Family & Domainsⁱ

Sequence cautionⁱ

Keywords - Coding sequence diversityⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ

Keywords - Technical termⁱ