UniProtKB - Q64693 (OBF1_MOUSE)
Protein
POU domain class 2-associating factor 1
Gene
Pou2af1
Organism
Mus musculus (Mouse)
Status
Functioni
Transcriptional coactivator that specifically associates with either POU2F1/OCT1 or POU2F2/OCT2. It boosts the POU2F1/OCT1 mediated promoter activity and to a lesser extent, that of POU2F2/OCT2. It has no intrinsic DNA-binding activity. It recognizes the POU domains of POU2F1/OCT1 and POU2F2/OCT2. It is essential for the response of B-cells to antigens and required for the formation of germinal centers (PubMed:23045607). Regulates IL6 expression in B cells as POU2F2/OCT2 coactivator (PubMed:23045607).1 Publication
GO - Molecular functioni
- DNA binding Source: MGI
- transcription coactivator activity Source: UniProtKB
GO - Biological processi
- cellular response to virus Source: UniProtKB
- germinal center B cell differentiation Source: UniProtKB
- positive regulation of interleukin-6 production Source: UniProtKB
- positive regulation of transcription by RNA polymerase II Source: MGI
Keywordsi
Biological process | Transcription, Transcription regulation |
Names & Taxonomyi
Protein namesi | Recommended name: POU domain class 2-associating factor 1CuratedAlternative name(s): B-cell-specific coactivator OBF-1 BOB-1 Short name: BOB1 OCA-B OCT-binding factor 1 |
Gene namesi | |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:105086, Pou2af1 |
Pathology & Biotechi
Disruption phenotypei
Mutants show severely reduced or absent germinal center B cells in the lung-draining lymphatic nodes when infected by influenza virus.1 Publication
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000058019 | 1 – 256 | POU domain class 2-associating factor 1Add BLAST | 256 |
Post-translational modificationi
Ubiquitinated; mediated by SIAH1 or SIAH2 and leading to its subsequent proteasomal degradation.By similarity
Keywords - PTMi
Ubl conjugationProteomic databases
PaxDbi | Q64693 |
PRIDEi | Q64693 |
ProteomicsDBi | 293823 |
PTM databases
iPTMneti | Q64693 |
PhosphoSitePlusi | Q64693 |
Expressioni
Tissue specificityi
B-cell specific.1 Publication
Inductioni
In B cells, expression is highly increased upon activation by LPS or CpG.1 Publication
Gene expression databases
Bgeei | ENSMUSG00000032053, Expressed in bone marrow and 127 other tissues |
ExpressionAtlasi | Q64693, baseline and differential |
Genevisiblei | Q64693, MM |
Interactioni
Subunit structurei
Interacts with POU2F1/OCT1 and POU2F2/OCT2; the interaction increases POU2F1 and POU2F2 transactivation activity.
By similarityBinary interactionsi
Hide detailsQ64693
With | #Exp. | IntAct |
---|---|---|
Syk [P48025] | 2 | EBI-943530,EBI-300116 |
SIAH1 [Q8IUQ4] from Homo sapiens. | 5 | EBI-943530,EBI-747107 |
Protein-protein interaction databases
DIPi | DIP-977N |
IntActi | Q64693, 3 interactors |
STRINGi | 10090.ENSMUSP00000034554 |
Miscellaneous databases
RNActi | Q64693, protein |
Family & Domainsi
Sequence similaritiesi
Belongs to the POU2AF1 family.Curated
Phylogenomic databases
eggNOGi | ENOG502R5JD, Eukaryota |
GeneTreei | ENSGT00390000017499 |
HOGENOMi | CLU_095920_0_0_1 |
InParanoidi | Q64693 |
OMAi | KPYQGVR |
OrthoDBi | 1172888at2759 |
PhylomeDBi | Q64693 |
TreeFami | TF332565 |
Family and domain databases
DisProti | DP00008 |
InterProi | View protein in InterPro IPR015389, PD-C2-AF1 |
Pfami | View protein in Pfam PF09310, PD-C2-AF1, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
Q64693-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MLWQKSTAPE QAPAPPRPYQ GVRVKEPVKE LLRRKRGHTS VGAAGPPTAV
60 70 80 90 100
VLPHQPLATY STVGPSCLDM EVSASTVTEE GTLCAGWLSQ PAPATLQPLA
110 120 130 140 150
PWTPYTEYVS HEAVSCPYST DMYVQPVCPS YTVVGPSSVL TYASPPLITN
160 170 180 190 200
VTPRSTATPA VGPQLEGPEH QAPLTYFPWP QPLSTLPTSS LQYQPPAPTL
210 220 230 240 250
SGPQFVQLPI SIPEPVLQDM DDPRRAISSL TIDKLLLEEE ESNTYELNHT
LSVEGF
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketA0A1L1SRM5 | A0A1L1SRM5_MOUSE | POU domain class 2-associating fact... | Pou2af1 | 150 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 50 | V → G in CAA91058 (PubMed:8530384).Curated | 1 | |
Sequence conflicti | 97 – 98 | QP → HA in CAA91058 (PubMed:8530384).Curated | 2 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Z54283 mRNA Translation: CAA91058.1 U43788 mRNA Translation: AAC52618.1 |
CCDSi | CCDS23176.1 |
PIRi | S63588 |
RefSeqi | NP_035266.1, NM_011136.2 |
Genome annotation databases
Ensembli | ENSMUST00000034554; ENSMUSP00000034554; ENSMUSG00000032053 |
GeneIDi | 18985 |
KEGGi | mmu:18985 |
UCSCi | uc009plg.1, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Z54283 mRNA Translation: CAA91058.1 U43788 mRNA Translation: AAC52618.1 |
CCDSi | CCDS23176.1 |
PIRi | S63588 |
RefSeqi | NP_035266.1, NM_011136.2 |
3D structure databases
SMRi | Q64693 |
ModBasei | Search... |
Protein-protein interaction databases
DIPi | DIP-977N |
IntActi | Q64693, 3 interactors |
STRINGi | 10090.ENSMUSP00000034554 |
PTM databases
iPTMneti | Q64693 |
PhosphoSitePlusi | Q64693 |
Proteomic databases
PaxDbi | Q64693 |
PRIDEi | Q64693 |
ProteomicsDBi | 293823 |
Protocols and materials databases
Antibodypediai | 4401, 605 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000034554; ENSMUSP00000034554; ENSMUSG00000032053 |
GeneIDi | 18985 |
KEGGi | mmu:18985 |
UCSCi | uc009plg.1, mouse |
Organism-specific databases
CTDi | 5450 |
MGIi | MGI:105086, Pou2af1 |
Phylogenomic databases
eggNOGi | ENOG502R5JD, Eukaryota |
GeneTreei | ENSGT00390000017499 |
HOGENOMi | CLU_095920_0_0_1 |
InParanoidi | Q64693 |
OMAi | KPYQGVR |
OrthoDBi | 1172888at2759 |
PhylomeDBi | Q64693 |
TreeFami | TF332565 |
Miscellaneous databases
BioGRID-ORCSi | 18985, 0 hits in 52 CRISPR screens |
ChiTaRSi | Pou2af1, mouse |
PROi | PR:Q64693 |
RNActi | Q64693, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000032053, Expressed in bone marrow and 127 other tissues |
ExpressionAtlasi | Q64693, baseline and differential |
Genevisiblei | Q64693, MM |
Family and domain databases
DisProti | DP00008 |
InterProi | View protein in InterPro IPR015389, PD-C2-AF1 |
Pfami | View protein in Pfam PF09310, PD-C2-AF1, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | OBF1_MOUSE | |
Accessioni | Q64693Primary (citable) accession number: Q64693 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 1, 1997 |
Last sequence update: | November 1, 1997 | |
Last modified: | April 7, 2021 | |
This is version 130 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families