UniProtKB - Q64691 (CAN3_MOUSE)
Calpain-3
Capn3
Functioni
Catalytic activityi
- Broad endopeptidase activity. EC:3.4.22.54
Activity regulationi
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 129 | PROSITE-ProRule annotation | 1 | |
Active sitei | 334 | PROSITE-ProRule annotation | 1 | |
Active sitei | 358 | PROSITE-ProRule annotation | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Calcium bindingi | 662 – 672 | 1By similarityAdd BLAST | 11 | |
Calcium bindingi | 705 – 716 | 2PROSITE-ProRule annotationBy similarityAdd BLAST | 12 | |
Calcium bindingi | 735 – 741 | 3PROSITE-ProRule annotationBy similarity | 7 | |
Calcium bindingi | 800 – 806 | 4By similarity | 7 |
GO - Molecular functioni
- calcium-dependent cysteine-type endopeptidase activity Source: MGI
- calcium ion binding Source: UniProtKB
- catalytic activity Source: UniProtKB
- enzyme binding Source: MGI
- ligase regulator activity Source: UniProtKB
- molecular adaptor activity Source: UniProtKB
- peptidase activity Source: UniProtKB
- signaling receptor binding Source: MGI
- sodium ion binding Source: UniProtKB
- structural constituent of muscle Source: UniProtKB
- titin binding Source: UniProtKB
GO - Biological processi
- calcium-dependent self proteolysis Source: UniProtKB
- cellular response to calcium ion Source: UniProtKB
- cellular response to salt stress Source: UniProtKB
- G1 to G0 transition involved in cell differentiation Source: UniProtKB
- muscle structure development Source: UniProtKB
- myofibril assembly Source: MGI
- negative regulation of apoptotic process Source: UniProtKB
- negative regulation of protein sumoylation Source: UniProtKB
- negative regulation of skeletal muscle cell differentiation Source: MGI
- negative regulation of transcription, DNA-templated Source: UniProtKB
- positive regulation of NF-kappaB transcription factor activity Source: UniProtKB
- positive regulation of proteolysis Source: UniProtKB
- positive regulation of release of sequestered calcium ion into cytosol Source: UniProtKB
- positive regulation of satellite cell activation involved in skeletal muscle regeneration Source: UniProtKB
- positive regulation of transcription, DNA-templated Source: UniProtKB
- programmed cell death Source: UniProtKB
- protein catabolic process Source: MGI
- protein-containing complex assembly Source: UniProtKB
- protein destabilization Source: MGI
- protein localization to membrane Source: UniProtKB
- proteolysis Source: UniProtKB
- regulation of catalytic activity Source: UniProtKB
- regulation of I-kappaB kinase/NF-kappaB signaling Source: UniProtKB
- regulation of myoblast differentiation Source: UniProtKB
- response to calcium ion Source: UniProtKB
- response to muscle activity Source: UniProtKB
- sarcomere organization Source: MGI
- self proteolysis Source: UniProtKB
- sodium-dependent self proteolysis Source: MGI
Keywordsi
Molecular function | Hydrolase, Protease, Thiol protease |
Ligand | Calcium, Metal-binding |
Enzyme and pathway databases
BRENDAi | 3.4.22.54 3474 |
Reactomei | R-MMU-1474228 Degradation of the extracellular matrix |
Protein family/group databases
MEROPSi | C02.004 |
Names & Taxonomyi
Protein namesi | Recommended name: Calpain-3 (EC:3.4.22.54)Alternative name(s): Calcium-activated neutral proteinase 3 Short name: CANP 3 Calpain L3 Calpain p94 Muscle-specific calcium-activated neutral protease 3 |
Gene namesi | Name:Capn3 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:107437 Capn3 |
Subcellular locationi
Other locations
Cytosol
- cytosol Source: UniProtKB
Nucleus
- nucleus Source: UniProtKB
Plasma Membrane
- plasma membrane Source: UniProtKB
- T-tubule Source: UniProtKB
Other locations
- cell Source: GOC
- cytoplasm Source: UniProtKB
- myofibril Source: UniProtKB
- protein-containing complex Source: UniProtKB
- Z disc Source: UniProtKB
Keywords - Cellular componenti
CytoplasmPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000207708 | 1 – 821 | Calpain-3Add BLAST | 821 |
Proteomic databases
PaxDbi | Q64691 |
PRIDEi | Q64691 |
PTM databases
iPTMneti | Q64691 |
PhosphoSitePlusi | Q64691 |
Expressioni
Gene expression databases
Bgeei | ENSMUSG00000079110 Expressed in 168 organ(s), highest expression level in lens of camera-type eye |
ExpressionAtlasi | Q64691 baseline and differential |
Genevisiblei | Q64691 MM |
Interactioni
Subunit structurei
Homodimer; via EF-hand domain 4.
Interacts with TTN/titin.
Interacts with CMYA5; this interaction, which results in CMYA5 proteolysis, may protect CAPN3 from autolysis.
By similarityGO - Molecular functioni
- enzyme binding Source: MGI
- signaling receptor binding Source: MGI
- titin binding Source: UniProtKB
Protein-protein interaction databases
BioGridi | 198472, 2 interactors |
STRINGi | 10090.ENSMUSP00000028749 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 74 – 417 | Calpain catalyticPROSITE-ProRule annotationAdd BLAST | 344 | |
Domaini | 649 – 683 | EF-hand 1PROSITE-ProRule annotationAdd BLAST | 35 | |
Domaini | 692 – 725 | EF-hand 2PROSITE-ProRule annotationAdd BLAST | 34 | |
Domaini | 722 – 757 | EF-hand 3PROSITE-ProRule annotationAdd BLAST | 36 | |
Domaini | 787 – 821 | EF-hand 4PROSITE-ProRule annotationAdd BLAST | 35 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 418 – 586 | Domain IIIAdd BLAST | 169 | |
Regioni | 587 – 649 | LinkerAdd BLAST | 63 | |
Regioni | 650 – 820 | Domain IVAdd BLAST | 171 |
Sequence similaritiesi
Keywords - Domaini
RepeatPhylogenomic databases
eggNOGi | KOG0045 Eukaryota ENOG410XP0B LUCA |
GeneTreei | ENSGT00940000156092 |
HOGENOMi | HOG000232035 |
InParanoidi | Q64691 |
KOi | K08573 |
OrthoDBi | 704215at2759 |
TreeFami | TF314748 |
Family and domain databases
CDDi | cd00214 Calpain_III, 1 hit cd00044 CysPc, 1 hit |
InterProi | View protein in InterPro IPR033883 C2_III IPR022684 Calpain_cysteine_protease IPR022682 Calpain_domain_III IPR022683 Calpain_III IPR036213 Calpain_III_sf IPR029531 CAPN3/9 IPR011992 EF-hand-dom_pair IPR018247 EF_Hand_1_Ca_BS IPR002048 EF_hand_dom IPR038765 Papain-like_cys_pep_sf IPR000169 Pept_cys_AS IPR001300 Peptidase_C2_calpain_cat |
PANTHERi | PTHR10183:SF329 PTHR10183:SF329, 1 hit |
Pfami | View protein in Pfam PF01067 Calpain_III, 1 hit PF13202 EF-hand_5, 1 hit PF00648 Peptidase_C2, 1 hit |
PRINTSi | PR00704 CALPAIN |
SMARTi | View protein in SMART SM00720 calpain_III, 1 hit SM00230 CysPc, 1 hit SM00054 EFh, 3 hits |
SUPFAMi | SSF47473 SSF47473, 1 hit SSF49758 SSF49758, 1 hit SSF54001 SSF54001, 1 hit |
PROSITEi | View protein in PROSITE PS50203 CALPAIN_CAT, 1 hit PS00018 EF_HAND_1, 2 hits PS50222 EF_HAND_2, 4 hits PS00139 THIOL_PROTEASE_CYS, 1 hit |
s (2+)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 2 described isoforms and 4 potential isoforms that are computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MPTVISPTVA PRTGAEPRSP GPVPHPAQGK TTEAGGGHPS GIYSAIISRN
60 70 80 90 100
FPIIGVKEKT FEQLRRKCLE KKVLYLDPEF PPDETSLFYS QKFPIQFVWK
110 120 130 140 150
RPPEICENPR FIIGGANRTD ICQGDLGDCW FLAAIACLTL NERLLFRVIP
160 170 180 190 200
HDQSFTENYA GIFHFQFWRY GDWVDVVIDD CLPTYNNQLV FTKSNHRNEF
210 220 230 240 250
WSALLEKAYA KLHGSYEALK GGNTTEAMED FTGGVTEFFE IKDAPSDMYK
260 270 280 290 300
IMRKAIERGS LMGCSIDDGT NMTYGTSPSG LNMGELIARM VRNMDNSLLR
310 320 330 340 350
DSDLDPRGSD DRPSRTIVPV QYETRMACGL VKGHAYSVTG LEEALFKGEK
360 370 380 390 400
VKLVRLRNPW GQVEWNGSWS DGWKDWSFVD KDEKARLQHQ VTEDGEFWMS
410 420 430 440 450
YDDFVYHFTK LEICNLTADA LESDKLQTWT VSVNEGRWVR GCSAGGCRNF
460 470 480 490 500
PDTFWTNPQY RLKLLEEDDD PEDSEVICSF LVALMQKNRR KDRKLGANLF
510 520 530 540 550
TIGFAIYEVP KEMHGNKQHL QKDFFLYNAS KARSKTYINM REVSQRFRLP
560 570 580 590 600
PSEYVIVPST YEPHQEGEFI LRVFSEKRNL SEEAENTISV DRPVKKKKNK
610 620 630 640 650
PIIFVSDRAN SNKELGVDQE AEEGKDKAGP EKRGETPQPR PGHTDQESEE
660 670 680 690 700
QQQFRNIFRQ IAGDDMEICA DELKNVLNTV VNKHKDLKTQ GFTLESCRSM
710 720 730 740 750
IALMDTDGSG RLNLQEFHHL WKKIKAWQKI FKHYDTDHSG TINSYEMRNA
760 770 780 790 800
VNDAGFHLNS QLYDIITMRY ADKHMNIDFD SFICCFVRLE GMFRAFNAFD
810 820
KDGDGIIKLN VLEWLQLTMY A
The sequence of this isoform differs from the canonical sequence as follows:
268-315: Missing.
595-638: Missing.
Computationally mapped potential isoform sequencesi
There are 4 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketQ0VGP9 | Q0VGP9_MOUSE | Calpain-3 | Capn3 | 709 | Annotation score: | ||
A2AVV5 | A2AVV5_MOUSE | Calpain-3 | Capn3 | 737 | Annotation score: | ||
A2AVV7 | A2AVV7_MOUSE | Calpain-3 | Capn3 | 757 | Annotation score: | ||
A2AVV6 | A2AVV6_MOUSE | Calpain-3 | Capn3 | 785 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 66 | R → K in CAA63301 (PubMed:8661728).Curated | 1 | |
Sequence conflicti | 66 | R → K in AAD28255 (Ref. 2) Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_005230 | 268 – 315 | Missing in isoform Short. 1 PublicationAdd BLAST | 48 | |
Alternative sequenceiVSP_005231 | 595 – 638 | Missing in isoform Short. 1 PublicationAdd BLAST | 44 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X92523 mRNA Translation: CAA63301.1 AF127766 mRNA Translation: AAD28255.2 AL935121 Genomic DNA No translation available. |
CCDSi | CCDS16620.1 [Q64691-1] CCDS50679.1 [Q64691-2] |
RefSeqi | NP_001171270.1, NM_001177799.1 [Q64691-2] NP_031627.2, NM_007601.3 [Q64691-1] |
Genome annotation databases
Ensembli | ENSMUST00000028749; ENSMUSP00000028749; ENSMUSG00000079110 [Q64691-1] ENSMUST00000110721; ENSMUSP00000106349; ENSMUSG00000079110 [Q64691-2] |
GeneIDi | 12335 |
KEGGi | mmu:12335 |
UCSCi | uc008lvz.2 mouse [Q64691-1] uc008lwa.2 mouse [Q64691-2] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X92523 mRNA Translation: CAA63301.1 AF127766 mRNA Translation: AAD28255.2 AL935121 Genomic DNA No translation available. |
CCDSi | CCDS16620.1 [Q64691-1] CCDS50679.1 [Q64691-2] |
RefSeqi | NP_001171270.1, NM_001177799.1 [Q64691-2] NP_031627.2, NM_007601.3 [Q64691-1] |
3D structure databases
SMRi | Q64691 |
ModBasei | Search... |
Protein-protein interaction databases
BioGridi | 198472, 2 interactors |
STRINGi | 10090.ENSMUSP00000028749 |
Protein family/group databases
MEROPSi | C02.004 |
PTM databases
iPTMneti | Q64691 |
PhosphoSitePlusi | Q64691 |
Proteomic databases
PaxDbi | Q64691 |
PRIDEi | Q64691 |
Genome annotation databases
Ensembli | ENSMUST00000028749; ENSMUSP00000028749; ENSMUSG00000079110 [Q64691-1] ENSMUST00000110721; ENSMUSP00000106349; ENSMUSG00000079110 [Q64691-2] |
GeneIDi | 12335 |
KEGGi | mmu:12335 |
UCSCi | uc008lvz.2 mouse [Q64691-1] uc008lwa.2 mouse [Q64691-2] |
Organism-specific databases
CTDi | 825 |
MGIi | MGI:107437 Capn3 |
Phylogenomic databases
eggNOGi | KOG0045 Eukaryota ENOG410XP0B LUCA |
GeneTreei | ENSGT00940000156092 |
HOGENOMi | HOG000232035 |
InParanoidi | Q64691 |
KOi | K08573 |
OrthoDBi | 704215at2759 |
TreeFami | TF314748 |
Enzyme and pathway databases
BRENDAi | 3.4.22.54 3474 |
Reactomei | R-MMU-1474228 Degradation of the extracellular matrix |
Miscellaneous databases
PROi | PR:Q64691 |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000079110 Expressed in 168 organ(s), highest expression level in lens of camera-type eye |
ExpressionAtlasi | Q64691 baseline and differential |
Genevisiblei | Q64691 MM |
Family and domain databases
CDDi | cd00214 Calpain_III, 1 hit cd00044 CysPc, 1 hit |
InterProi | View protein in InterPro IPR033883 C2_III IPR022684 Calpain_cysteine_protease IPR022682 Calpain_domain_III IPR022683 Calpain_III IPR036213 Calpain_III_sf IPR029531 CAPN3/9 IPR011992 EF-hand-dom_pair IPR018247 EF_Hand_1_Ca_BS IPR002048 EF_hand_dom IPR038765 Papain-like_cys_pep_sf IPR000169 Pept_cys_AS IPR001300 Peptidase_C2_calpain_cat |
PANTHERi | PTHR10183:SF329 PTHR10183:SF329, 1 hit |
Pfami | View protein in Pfam PF01067 Calpain_III, 1 hit PF13202 EF-hand_5, 1 hit PF00648 Peptidase_C2, 1 hit |
PRINTSi | PR00704 CALPAIN |
SMARTi | View protein in SMART SM00720 calpain_III, 1 hit SM00230 CysPc, 1 hit SM00054 EFh, 3 hits |
SUPFAMi | SSF47473 SSF47473, 1 hit SSF49758 SSF49758, 1 hit SSF54001 SSF54001, 1 hit |
PROSITEi | View protein in PROSITE PS50203 CALPAIN_CAT, 1 hit PS00018 EF_HAND_1, 2 hits PS50222 EF_HAND_2, 4 hits PS00139 THIOL_PROTEASE_CYS, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | CAN3_MOUSE | |
Accessioni | Q64691Primary (citable) accession number: Q64691 Secondary accession number(s): A2AVV3, Q9WUC5 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 1, 1997 |
Last sequence update: | July 27, 2011 | |
Last modified: | November 13, 2019 | |
This is version 173 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Complete proteome, Reference proteomeDocuments
- Peptidase families
Classification of peptidase families and list of entries - SIMILARITY comments
Index of protein domains and families - MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot