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Protein

P2X purinoceptor 7

Gene

P2rx7

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Receptor for ATP that acts as a ligand-gated ion channel. Responsible for ATP-dependent lysis of macrophages through the formation of membrane pores permeable to large molecules. Could function in both fast synaptic transmission and the ATP-mediated lysis of antigen-presenting cells.

GO - Molecular functioni

  • ATP binding Source: RGD
  • channel activity Source: MGI
  • copper ion binding Source: RGD
  • extracellularly ATP-gated cation channel activity Source: BHF-UCL
  • magnesium ion binding Source: RGD
  • protein serine/threonine kinase activator activity Source: RGD
  • purinergic nucleotide receptor activity Source: BHF-UCL
  • scaffold protein binding Source: RGD
  • signaling receptor binding Source: BHF-UCL
  • zinc ion binding Source: RGD

GO - Biological processi

  • apoptotic signaling pathway Source: BHF-UCL
  • bleb assembly Source: BHF-UCL
  • cell surface receptor signaling pathway Source: BHF-UCL
  • membrane depolarization Source: BHF-UCL
  • neuronal action potential Source: RGD
  • organic cation transport Source: RGD
  • pore complex assembly Source: RGD
  • positive regulation of cytolysis Source: BHF-UCL
  • positive regulation of cytoskeleton organization Source: BHF-UCL
  • positive regulation of interleukin-1 beta secretion Source: RGD
  • positive regulation of protein phosphorylation Source: RGD
  • regulation of sodium ion transport Source: BHF-UCL
  • response to ATP Source: BHF-UCL

Keywordsi

Molecular functionIon channel, Ligand-gated ion channel, Receptor
Biological processIon transport, Transport

Enzyme and pathway databases

ReactomeiR-RNO-139853 Elevation of cytosolic Ca2+ levels
R-RNO-418346 Platelet homeostasis
R-RNO-844456 The NLRP3 inflammasome

Names & Taxonomyi

Protein namesi
Recommended name:
P2X purinoceptor 7
Short name:
P2X7
Alternative name(s):
ATP receptor
P2Z receptor
Purinergic receptor
Gene namesi
Name:P2rx7
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 12

Organism-specific databases

RGDi3241 P2rx7

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 25CytoplasmicSequence analysisAdd BLAST25
Transmembranei26 – 46Helical; Name=1Sequence analysisAdd BLAST21
Topological domaini47 – 334ExtracellularSequence analysisAdd BLAST288
Transmembranei335 – 355Helical; Name=2Sequence analysisAdd BLAST21
Topological domaini356 – 595CytoplasmicSequence analysisAdd BLAST240

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL2496
GuidetoPHARMACOLOGYi484

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001615621 – 595P2X purinoceptor 7Add BLAST595

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi74N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi119 ↔ 168By similarity
Modified residuei125ADP-ribosylarginineBy similarity1
Disulfide bondi129 ↔ 152By similarity
Disulfide bondi135 ↔ 162By similarity
Glycosylationi187N-linked (GlcNAc...) asparagineCombined sources1
Glycosylationi202N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi213N-linked (GlcNAc...) asparagineCombined sources1
Disulfide bondi216 ↔ 226By similarity
Glycosylationi241N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi260 ↔ 269By similarity
Glycosylationi284N-linked (GlcNAc...) asparagineSequence analysis1

Post-translational modificationi

Phosphorylation results in its inactivation.By similarity
ADP-ribosylation at Arg-125 is necessary and sufficient to activate P2RX7 and gate the channel.By similarity
Palmitoylation of several cysteines in the C-terminal cytoplasmic tail is required for efficient localization to cell surface.By similarity

Keywords - PTMi

ADP-ribosylation, Disulfide bond, Glycoprotein, Lipoprotein, Palmitate, Phosphoprotein

Proteomic databases

PaxDbiQ64663
PRIDEiQ64663

PTM databases

iPTMnetiQ64663
PhosphoSitePlusiQ64663
SwissPalmiQ64663
UniCarbKBiQ64663

Expressioni

Gene expression databases

BgeeiENSRNOG00000001296
ExpressionAtlasiQ64663 baseline and differential
GenevisibleiQ64663 RN

Interactioni

Subunit structurei

Functional P2XRs are organized as homomeric and heteromeric trimers. Interacts with LAMA3, ITGB2, ACTB, ACTN4, SVIL, MPP3, HSPA1, HSPCB, HSPA8, PIK230 and PTPRB (By similarity). Interacts (via C-terminus) with EMP2 (By similarity).By similarity

GO - Molecular functioni

  • scaffold protein binding Source: RGD
  • signaling receptor binding Source: BHF-UCL

Protein-protein interaction databases

BioGridi248286, 11 interactors
CORUMiQ64663
IntActiQ64663, 2 interactors
MINTiQ64663
STRINGi10116.ENSRNOP00000001746

Chemistry databases

BindingDBiQ64663

Structurei

3D structure databases

SMRiQ64663
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the P2X receptor family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IK4U Eukaryota
ENOG4110DDY LUCA
GeneTreeiENSGT00390000016028
HOGENOMiHOG000232042
HOVERGENiHBG053086
InParanoidiQ64663
KOiK05220
PhylomeDBiQ64663
TreeFamiTF328633

Family and domain databases

Gene3Di2.60.490.10, 1 hit
InterProiView protein in InterPro
IPR003050 P2X7_purinoceptor
IPR027309 P2X_extracellular_dom_sf
IPR001429 P2X_purnocptor
PANTHERiPTHR10125 PTHR10125, 1 hit
PTHR10125:SF13 PTHR10125:SF13, 1 hit
PfamiView protein in Pfam
PF00864 P2X_receptor, 1 hit
PRINTSiPR01314 P2X7RECEPTOR
PR01307 P2XRECEPTOR
TIGRFAMsiTIGR00863 P2X, 1 hit
PROSITEiView protein in PROSITE
PS01212 P2X_RECEPTOR, 1 hit

Sequencei

Sequence statusi: Complete.

Q64663-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPACCSWNDV FQYETNKVTR IQSVNYGTIK WILHMTVFSY VSFALMSDKL
60 70 80 90 100
YQRKEPLISS VHTKVKGVAE VTENVTEGGV TKLVHGIFDT ADYTLPLQGN
110 120 130 140 150
SFFVMTNYLK SEGQEQKLCP EYPSRGKQCH SDQGCIKGWM DPQSKGIQTG
160 170 180 190 200
RCIPYDQKRK TCEIFAWCPA EEGKEAPRPA LLRSAENFTV LIKNNIDFPG
210 220 230 240 250
HNYTTRNILP GMNISCTFHK TWNPQCPIFR LGDIFQEIGE NFTEVAVQGG
260 270 280 290 300
IMGIEIYWDC NLDSWSHRCQ PKYSFRRLDD KYTNESLFPG YNFRYAKYYK
310 320 330 340 350
ENGMEKRTLI KAFGVRFDIL VFGTGGKFDI IQLVVYIGST LSYFGLATVC
360 370 380 390 400
IDLIINTYAS TCCRSRVYPS CKCCEPCAVN EYYYRKKCEP IVEPKPTLKY
410 420 430 440 450
VSFVDEPHIW MVDQQLLGKS LQDVKGQEVP RPQTDFLELS RLSLSLHHSP
460 470 480 490 500
PIPGQPEEMQ LLQIEAVPRS RDSPDWCQCG NCLPSQLPEN RRALEELCCR
510 520 530 540 550
RKPGQCITTS ELFSKIVLSR EALQLLLLYQ EPLLALEGEA INSKLRHCAY
560 570 580 590
RSYATWRFVS QDMADFAILP SCCRWKIRKE FPKTQGQYSG FKYPY
Length:595
Mass (Da):68,392
Last modified:November 1, 1996 - v1
Checksum:i4A6DD6058E5988D3
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X95882 mRNA Translation: CAA65131.1
RefSeqiNP_062129.1, NM_019256.1
UniGeneiRn.10510

Genome annotation databases

EnsembliENSRNOT00000001746; ENSRNOP00000001746; ENSRNOG00000001296
GeneIDi29665
KEGGirno:29665
UCSCiRGD:3241 rat

Similar proteinsi

Entry informationi

Entry nameiP2RX7_RAT
AccessioniPrimary (citable) accession number: Q64663
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: June 20, 2018
This is version 140 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

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