UniProtKB - Q64658 (C11B2_MESAU)
Cytochrome P450 11B2, mitochondrial
CYP11B2
Functioni
A cytochrome P450 monooxygenase that catalyzes the biosynthesis of adrenal mineralocorticoid aldosterone. Catalyzes three sequential oxidative reactions of 11-deoxycorticosterone/21-hydroxyprogesterone, namely 11-beta hydroxylation followed with two successive oxidations at C18 to yield 18-hydroxy and then 18-aldehyde derivatives, resulting in the formation of aldosterone. Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate and reducing the second into a water molecule. Two electrons are provided by NADPH via a two-protein mitochondrial transfer system comprising flavoprotein FDXR (adrenodoxin/ferredoxin reductase) and nonheme iron-sulfur protein FDX1 or FDX2 (adrenodoxin/ferredoxin).
By similarityCatalytic activityi
- a steroid + 2 H+ + O2 + 2 reduced [adrenodoxin] = an 11β-hydroxysteroid + H2O + 2 oxidized [adrenodoxin]By similarityEC:1.14.15.4By similarityThis reaction proceeds in the forwardBy similarity direction.
- 21-hydroxyprogesterone + 2 H+ + O2 + 2 reduced [adrenodoxin] = corticosterone + H2O + 2 oxidized [adrenodoxin]By similarityThis reaction proceeds in the forwardBy similarity direction.
- corticosterone + 2 H+ + O2 + 2 reduced [adrenodoxin] = 18-hydroxycorticosterone + H2O + 2 oxidized [adrenodoxin]By similarityEC:1.14.15.5By similarityThis reaction proceeds in the forwardBy similarity direction.
- 18-hydroxycorticosterone + 2 H+ + O2 + 2 reduced [adrenodoxin] = aldosterone + 2 H2O + 2 oxidized [adrenodoxin]By similarityThis reaction proceeds in the forwardBy similarity direction.
- 11-deoxycortisol + 2 H+ + O2 + 2 reduced [adrenodoxin] = cortisol + H2O + 2 oxidized [adrenodoxin]By similarityThis reaction proceeds in the forwardBy similarity direction.
Cofactori
: Steroid biosynthesis Pathwayi
This protein is involved in Steroid biosynthesis.By similarityView all proteins of this organism that are known to be involved in Steroid biosynthesis.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 381 | 11-deoxycorticosterone; via carbonyl oxygenBy similarity | 1 | |
Metal bindingi | 447 | Iron (heme axial ligand)By similarity | 1 |
GO - Molecular functioni
- corticosterone 18-monooxygenase activity Source: UniProtKB-EC
- heme binding Source: UniProtKB
- iron ion binding Source: InterPro
- steroid 11-beta-monooxygenase activity Source: UniProtKB
GO - Biological processi
- aldosterone biosynthetic process Source: UniProtKB
Keywordsi
Molecular function | Monooxygenase, Oxidoreductase |
Biological process | Steroidogenesis |
Ligand | Heme, Iron, Metal-binding |
Names & Taxonomyi
Protein namesi | Recommended name: Cytochrome P450 11B2, mitochondrialAlternative name(s): |
Gene namesi | Name:CYP11B2 |
Organismi | Mesocricetus auratus (Golden hamster) |
Taxonomic identifieri | 10036 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Cricetidae › Cricetinae › Mesocricetus |
Proteomesi |
|
Subcellular locationi
Mitochondrion
- Mitochondrion inner membrane By similarity; Peripheral membrane protein By similarity
Mitochondrion
- mitochondrial inner membrane Source: UniProtKB-SubCell
Keywords - Cellular componenti
Membrane, Mitochondrion, Mitochondrion inner membranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transit peptidei | 1 – 24 | MitochondrionBy similarityAdd BLAST | 24 | |
ChainiPRO_0000003599 | 25 – 500 | Cytochrome P450 11B2, mitochondrialAdd BLAST | 476 |
Expressioni
Tissue specificityi
Inductioni
Family & Domainsi
Sequence similaritiesi
Keywords - Domaini
Transit peptidePhylogenomic databases
eggNOGi | KOG0159, Eukaryota |
Family and domain databases
Gene3Di | 1.10.630.10, 1 hit |
InterProi | View protein in InterPro IPR001128, Cyt_P450 IPR017972, Cyt_P450_CS IPR002399, Cyt_P450_mitochondrial IPR036396, Cyt_P450_sf |
Pfami | View protein in Pfam PF00067, p450, 1 hit |
PRINTSi | PR00408, MITP450 PR00385, P450 |
SUPFAMi | SSF48264, SSF48264, 1 hit |
PROSITEi | View protein in PROSITE PS00086, CYTOCHROME_P450, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
10 20 30 40 50
MALRAKADVW LARPWQCLPR TRALGTTAAL APNTLRPFEA IPQYSRNRWL
60 70 80 90 100
KMLQILREEG QEGLHLEMHE AFRELGPIFR YSMGRTQVVS VMLPEDAEKL
110 120 130 140 150
HQVESMHPRR MHLEPWVAHR EHRGLSRGVF LLNGPEWRFN RLRLNPHVLS
160 170 180 190 200
PKAVQKFVPM VDMVARDFLE SLKKKVFQNA RGSLTMDVQQ SLFNYSIEAS
210 220 230 240 250
NFVLFGERLG LLGHDLSPAS LTFIHALHSV FKTTPQLMFL PRSLTRWTST
260 270 280 290 300
RVWKEHFEAW DVISEYVNRC IRKVHQELRL GSPHTYSGIV AELMSQGALP
310 320 330 340 350
LDAIRANSIE LTAGSVDTTT FPLVMALFEL ARNPDVQQAV RQESLAAEAS
360 370 380 390 400
VAANPQRAMS DLPLLRAVLK ETLRLYPVGG FLERILSSDL VLQNYHVPAG
410 420 430 440 450
TLVLLYLYSM GRNPAVFPRP EHYLPQRWLE RNGSFQHLTF GFGVRQCLGK
460 470 480 490 500
RLAQVEMLLL LHHVLKSFRV ETQEREDVRM VYRFVLAPSS SPLLTFRPVS
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | S73810 mRNA Translation: AAB31349.1 U71280 Genomic DNA Translation: AAB16805.1 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | S73810 mRNA Translation: AAB31349.1 U71280 Genomic DNA Translation: AAB16805.1 |
3D structure databases
SMRi | Q64658 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 10036.XP_005086076.1 |
Phylogenomic databases
eggNOGi | KOG0159, Eukaryota |
Family and domain databases
Gene3Di | 1.10.630.10, 1 hit |
InterProi | View protein in InterPro IPR001128, Cyt_P450 IPR017972, Cyt_P450_CS IPR002399, Cyt_P450_mitochondrial IPR036396, Cyt_P450_sf |
Pfami | View protein in Pfam PF00067, p450, 1 hit |
PRINTSi | PR00408, MITP450 PR00385, P450 |
SUPFAMi | SSF48264, SSF48264, 1 hit |
PROSITEi | View protein in PROSITE PS00086, CYTOCHROME_P450, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | C11B2_MESAU | |
Accessioni | Q64658Primary (citable) accession number: Q64658 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 15, 1998 |
Last sequence update: | November 1, 1996 | |
Last modified: | August 12, 2020 | |
This is version 88 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families