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Protein

Lanosterol 14-alpha demethylase

Gene

Cyp51a1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes C14-demethylation of lanosterol; it transforms lanosterol into 4,4'-dimethyl cholesta-8,14,24-triene-3-beta-ol.1 Publication

Catalytic activityi

A 14-alpha-methylsteroid + 3 O2 + 3 NADPH = a Delta(14)-steroid + formate + 3 NADP+ + 4 H2O.1 Publication

Cofactori

hemeBy similarity

Enzyme regulationi

Inhibited by azalanstat.1 Publication

Pathwayi: zymosterol biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes zymosterol from lanosterol.1 Publication
Proteins known to be involved in the 6 steps of the subpathway in this organism are:
  1. Lanosterol 14-alpha demethylase (Cyp51a1)
  2. no protein annotated in this organism
  3. Methylsterol monooxygenase 1 (Msmo1)
  4. Sterol-4-alpha-carboxylate 3-dehydrogenase, decarboxylating (Nsdhl)
  5. 3-keto-steroid reductase (Hsd17b7)
  6. no protein annotated in this organism
This subpathway is part of the pathway zymosterol biosynthesis, which is itself part of Steroid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes zymosterol from lanosterol, the pathway zymosterol biosynthesis and in Steroid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi449Iron (heme axial ligand)By similarity1

GO - Molecular functioni

  • heme binding Source: InterPro
  • iron ion binding Source: InterPro
  • sterol 14-demethylase activity Source: RGD

GO - Biological processi

  • cholesterol biosynthetic process via 24,25-dihydrolanosterol Source: RGD
  • regulation of cholesterol biosynthetic process Source: RGD

Keywordsi

Molecular functionMonooxygenase, Oxidoreductase
Biological processCholesterol biosynthesis, Cholesterol metabolism, Lipid biosynthesis, Lipid metabolism, Steroid biosynthesis, Steroid metabolism, Sterol biosynthesis, Sterol metabolism
LigandHeme, Iron, Metal-binding, NADP

Enzyme and pathway databases

BRENDAi1.14.13.70 5301
ReactomeiR-RNO-191273 Cholesterol biosynthesis
R-RNO-211976 Endogenous sterols
UniPathwayiUPA00770; UER00754

Chemistry databases

SwissLipidsiSLP:000001302

Names & Taxonomyi

Protein namesi
Recommended name:
Lanosterol 14-alpha demethylase (EC:1.14.13.701 Publication)
Short name:
LDM
Alternative name(s):
CYPLI
Cytochrome P450 51A1
Cytochrome P450-14DM
Short name:
Cytochrome P45014DM
Cytochrome P450LI
Sterol 14-alpha demethylase
Gene namesi
Name:Cyp51a1
Synonyms:Cyp51
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 4

Organism-specific databases

RGDi2481 Cyp51

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei24 – 44HelicalSequence analysisAdd BLAST21

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane, Microsome

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL4981

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000520011 – 503Lanosterol 14-alpha demethylaseAdd BLAST503

Proteomic databases

PaxDbiQ64654
PRIDEiQ64654

Expressioni

Gene expression databases

BgeeiENSRNOG00000007234
GenevisibleiQ64654 RN

Interactioni

Protein-protein interaction databases

BioGridi247462, 1 interactor
STRINGi10116.ENSRNOP00000009985

Chemistry databases

BindingDBiQ64654

Structurei

3D structure databases

ProteinModelPortaliQ64654
SMRiQ64654
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the cytochrome P450 family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0684 Eukaryota
COG2124 LUCA
GeneTreeiENSGT00800000124060
HOGENOMiHOG000042780
HOVERGENiHBG051102
InParanoidiQ64654
KOiK05917
OMAiAPIHSIM
OrthoDBiEOG091G04XH
PhylomeDBiQ64654
TreeFamiTF105091

Family and domain databases

Gene3Di1.10.630.10, 1 hit
InterProiView protein in InterPro
IPR001128 Cyt_P450
IPR017972 Cyt_P450_CS
IPR002403 Cyt_P450_E_grp-IV
IPR036396 Cyt_P450_sf
PfamiView protein in Pfam
PF00067 p450, 1 hit
PRINTSiPR00465 EP450IV
PR00385 P450
SUPFAMiSSF48264 SSF48264, 1 hit
PROSITEiView protein in PROSITE
PS00086 CYTOCHROME_P450, 1 hit

Sequencei

Sequence statusi: Complete.

Q64654-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVLLGLLQSG GSVLGQAMEQ VTGGNLLSTL LIACAFTLSL VYLFRLAVGH
60 70 80 90 100
MVQLPAGAKS PPYIYSPIPF LGHAIAFGKS PIEFLENAYE KYGPVFSFTM
110 120 130 140 150
VGKTFTYLLG SDAAALLFNS KNEDLNAEEV YGRLTTPVFG KGVAYDVPNA
160 170 180 190 200
VFLEQKKILK SGLNIAHFKQ YVSIIEKEAK EYFKSWGESG ERNVFEALSE
210 220 230 240 250
LIILTASHCL HGKEIRSQLN EKVAQLYADL DGGFSHAAWL LPGWLPLPSF
260 270 280 290 300
RRRDRAHREI KNIFYKAIQK RRLSKEPAED ILQTLLDSTY KDGRPLTDDE
310 320 330 340 350
IAGMLIGLLL AGQHTSSTTS AWMGFFLARD KPLQDKCYLE QKTVCGEDLP
360 370 380 390 400
PLTYEQLKDL NLLDRCIKET LRLRPPIMTM MRMAKTPQTV AGYTIPPGHQ
410 420 430 440 450
VCVSPTVNQR LKDSWVERLD FNPDRYLQDN PASGEKFAYV PFGAGRHRCI
460 470 480 490 500
GENFAYVQIK TIWSTMLRLY EFDLINGYFP SVNYTTMIHT PENPVIRYKR

RSK
Length:503
Mass (Da):56,707
Last modified:November 1, 1996 - v1
Checksum:i33D8F345FFE9CF21
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti181E → K in AAA87074 (PubMed:7665087).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D55681 mRNA Translation: BAA09529.1
AB004096 Genomic DNA Translation: BAA20354.1
BC087033 mRNA Translation: AAH87033.1
U17697 mRNA Translation: AAA87074.1
PIRiJC4240
JC4758
RefSeqiNP_037073.1, NM_012941.2
UniGeneiRn.107152

Genome annotation databases

EnsembliENSRNOT00000009985; ENSRNOP00000009985; ENSRNOG00000007234
GeneIDi25427
KEGGirno:25427
UCSCiRGD:2481 rat

Similar proteinsi

Entry informationi

Entry nameiCP51A_RAT
AccessioniPrimary (citable) accession number: Q64654
Secondary accession number(s): Q64549
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: June 20, 2018
This is version 148 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

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