UniProtKB - Q64632 (ITB4_RAT)
Integrin beta-4
Itgb4
Functioni
Integrin alpha-6/beta-4 is a receptor for laminin. It plays a critical structural role in the hemidesmosome of epithelial cells. Is required for the regulation of keratinocyte polarity and motility. ITGA6:ITGB4 binds to NRG1 (via EGF domain) and this binding is essential for NRG1-ERBB signaling. ITGA6:ITGB4 binds to IGF1 and this binding is essential for IGF1 signaling. ITGA6:ITGB4 binds to IGF2 and this binding is essential for IGF2 signaling.
By similarityGO - Molecular functioni
- cell adhesion molecule binding Source: RGD
- G protein-coupled receptor binding Source: RGD
- integrin binding Source: GO_Central
GO - Biological processi
- autophagy Source: RGD
- cell adhesion Source: RGD
- cell adhesion mediated by integrin Source: GO_Central
- cell-matrix adhesion Source: RGD
- cell migration Source: GO_Central
- cell motility Source: UniProtKB
- filopodium assembly Source: RGD
- hemidesmosome assembly Source: RGD
- integrin-mediated signaling pathway Source: GO_Central
- mesodermal cell differentiation Source: RGD
- myelination in peripheral nervous system Source: RGD
- nail development Source: RGD
- peripheral nervous system myelin formation Source: RGD
- response to wounding Source: UniProtKB
- skin morphogenesis Source: RGD
- trophoblast cell migration Source: RGD
Keywordsi
Molecular function | Integrin, Receptor |
Biological process | Cell adhesion |
Enzyme and pathway databases
Reactomei | R-RNO-2022090, Assembly of collagen fibrils and other multimeric structures R-RNO-3000157, Laminin interactions R-RNO-3000170, Syndecan interactions R-RNO-446107, Type I hemidesmosome assembly |
Names & Taxonomyi
Protein namesi | Recommended name: Integrin beta-4Alternative name(s): GP150 CD_antigen: CD104 |
Gene namesi | Name:Itgb4 |
Organismi | Rattus norvegicus (Rat) |
Taxonomic identifieri | 10116 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Rattus |
Proteomesi |
|
Organism-specific databases
RGDi | 2928, Itgb4 |
Subcellular locationi
Plasma membrane
- Cell membrane By similarity; Single-pass type I membrane protein By similarity
- Cell membrane By similarity; Lipid-anchor By similarity
Other locations
- hemidesmosome By similarity
Note: Colocalizes with DST at the leading edge of migrating keratinocytes.By similarity
Extracellular region or secreted
- extracellular space Source: RGD
Plasma Membrane
- basal plasma membrane Source: RGD
- integrin complex Source: RGD
- plasma membrane Source: RGD
Other locations
- basement membrane Source: RGD
- cell cortex Source: RGD
- cell leading edge Source: UniProtKB
- cell surface Source: RGD
- cytoplasm Source: RGD
- focal adhesion Source: GO_Central
- hemidesmosome Source: RGD
- integral component of membrane Source: RGD
- membrane Source: RGD
- receptor complex Source: RGD
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 28 – 713 | ExtracellularSequence analysisAdd BLAST | 686 | |
Transmembranei | 714 – 734 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 735 – 1807 | CytoplasmicSequence analysisAdd BLAST | 1073 |
Keywords - Cellular componenti
Cell junction, Cell membrane, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 27 | By similarityAdd BLAST | 27 | |
ChainiPRO_0000016347 | 28 – 1807 | Integrin beta-4Add BLAST | 1780 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Disulfide bondi | 30 ↔ 456 | By similarity | ||
Disulfide bondi | 38 ↔ 48 | By similarity | ||
Disulfide bondi | 41 ↔ 72 | By similarity | ||
Disulfide bondi | 51 ↔ 61 | By similarity | ||
Disulfide bondi | 245 ↔ 288 | By similarity | ||
Glycosylationi | 327 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 424 ↔ 673 | By similarity | ||
Disulfide bondi | 453 ↔ 458 | By similarity | ||
Disulfide bondi | 469 ↔ 480 | By similarity | ||
Disulfide bondi | 477 ↔ 513 | By similarity | ||
Disulfide bondi | 482 ↔ 491 | By similarity | ||
Glycosylationi | 492 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 493 ↔ 504 | By similarity | ||
Disulfide bondi | 519 ↔ 524 | By similarity | ||
Disulfide bondi | 521 ↔ 552 | By similarity | ||
Disulfide bondi | 526 ↔ 537 | By similarity | ||
Disulfide bondi | 558 ↔ 563 | By similarity | ||
Disulfide bondi | 565 ↔ 574 | By similarity | ||
Disulfide bondi | 576 ↔ 583 | By similarity | ||
Glycosylationi | 580 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 597 ↔ 602 | By similarity | ||
Disulfide bondi | 599 ↔ 650 | By similarity | ||
Disulfide bondi | 604 ↔ 616 | By similarity | ||
Glycosylationi | 619 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 628 ↔ 637 | By similarity | ||
Disulfide bondi | 634 ↔ 708 | By similarity | ||
Disulfide bondi | 653 ↔ 682 | By similarity | ||
Glycosylationi | 697 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Modified residuei | 773 | PhosphoserineCombined sources | 1 | |
Modified residuei | 1071 | PhosphoserineCombined sources | 1 | |
Modified residuei | 1121 | PhosphoserineCombined sources | 1 | |
Modified residuei | 1386 | PhosphoserineCombined sources | 1 | |
Modified residuei | 1389 | PhosphoserineCombined sources | 1 | |
Modified residuei | 1405 | PhosphoserineBy similarity | 1 | |
Modified residuei | 1418 | PhosphothreonineBy similarity | 1 | |
Modified residuei | 1425 | PhosphoserineCombined sources | 1 | |
Modified residuei | 1514 | PhosphothreonineBy similarity | 1 | |
Modified residuei | 1776 | PhosphoserineCombined sources | 1 |
Post-translational modificationi
Keywords - PTMi
Disulfide bond, Glycoprotein, Lipoprotein, Palmitate, PhosphoproteinProteomic databases
PaxDbi | Q64632 |
PRIDEi | Q64632 |
PTM databases
GlyGeni | Q64632, 5 sites |
iPTMneti | Q64632 |
PhosphoSitePlusi | Q64632 |
Interactioni
Subunit structurei
Heterodimer of an alpha and a beta subunit. Beta-4 associates with alpha-6.
Interacts (via cytoplasmic region) with COL17A1 (via cytoplasmic region).
Interacts (via cytoplasmic region) with DST isoform 3 (via N-terminus).
Interacts (via cytoplasmic domain) with DST (via N-terminus).
Interacts with RAC1. ITGA6:ITGB4 is found in a ternary complex with NRG1 and ERBB3. ITGA6:ITGB4 is found in a ternary complex with IGF1 and IGF1R. ITGA6:ITGB4 interacts with IGF2.
By similarityGO - Molecular functioni
- cell adhesion molecule binding Source: RGD
- G protein-coupled receptor binding Source: RGD
- integrin binding Source: GO_Central
Protein-protein interaction databases
STRINGi | 10116.ENSRNOP00000059783 |
Structurei
Secondary structure
3D structure databases
SMRi | Q64632 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 29 – 73 | PSIAdd BLAST | 45 | |
Domaini | 131 – 340 | VWFAAdd BLAST | 210 | |
Repeati | 457 – 503 | IAdd BLAST | 47 | |
Repeati | 504 – 543 | IIAdd BLAST | 40 | |
Repeati | 544 – 582 | IIIAdd BLAST | 39 | |
Repeati | 583 – 621 | IVAdd BLAST | 39 | |
Domaini | 981 – 1086 | Calx-betaAdd BLAST | 106 | |
Domaini | 1131 – 1220 | Fibronectin type-III 1PROSITE-ProRule annotationAdd BLAST | 90 | |
Domaini | 1224 – 1323 | Fibronectin type-III 2PROSITE-ProRule annotationAdd BLAST | 100 | |
Domaini | 1514 – 1609 | Fibronectin type-III 3PROSITE-ProRule annotationAdd BLAST | 96 | |
Domaini | 1627 – 1723 | Fibronectin type-III 4PROSITE-ProRule annotationAdd BLAST | 97 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 194 – 199 | Involved in NRG1- and IGF1-bindingBy similarity | 6 | |
Regioni | 457 – 621 | Cysteine-rich tandem repeatsAdd BLAST | 165 | |
Regioni | 734 – 751 | Palmitoylated on several cysteinesBy similarityAdd BLAST | 18 | |
Regioni | 1119 – 1141 | DisorderedSequence analysisAdd BLAST | 23 |
Domaini
Sequence similaritiesi
Keywords - Domaini
Repeat, Signal, Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG1226, Eukaryota |
InParanoidi | Q64632 |
OrthoDBi | 473040at2759 |
PhylomeDBi | Q64632 |
Family and domain databases
CDDi | cd00063, FN3, 4 hits |
Gene3Di | 2.60.40.10, 4 hits 2.60.40.2030, 1 hit 3.40.50.410, 1 hit |
InterProi | View protein in InterPro IPR038081, CalX-like_sf IPR003644, Calx_beta IPR000742, EGF-like_dom IPR003961, FN3_dom IPR036116, FN3_sf IPR040622, I-EGF_1 IPR013783, Ig-like_fold IPR033760, Integrin_beta_N IPR015812, Integrin_bsu IPR012013, Integrin_bsu-4 IPR012896, Integrin_bsu_tail IPR036349, Integrin_bsu_tail_dom_sf IPR002369, Integrin_bsu_VWA IPR036465, vWFA_dom_sf |
PANTHERi | PTHR10082, PTHR10082, 3 hits PTHR10082:SF42, PTHR10082:SF42, 3 hits |
Pfami | View protein in Pfam PF03160, Calx-beta, 1 hit PF00041, fn3, 4 hits PF18372, I-EGF_1, 1 hit PF07965, Integrin_B_tail, 1 hit PF00362, Integrin_beta, 1 hit PF17205, PSI_integrin, 1 hit |
PIRSFi | PIRSF002513, Integrin_B4, 1 hit |
PRINTSi | PR01186, INTEGRINB |
SMARTi | View protein in SMART SM00237, Calx_beta, 1 hit SM00060, FN3, 4 hits SM00187, INB, 1 hit SM01242, Integrin_B_tail, 1 hit |
SUPFAMi | SSF141072, SSF141072, 1 hit SSF49265, SSF49265, 2 hits SSF53300, SSF53300, 1 hit SSF69687, SSF69687, 1 hit |
PROSITEi | View protein in PROSITE PS00022, EGF_1, 2 hits PS01186, EGF_2, 2 hits PS50853, FN3, 4 hits PS00243, INTEGRIN_BETA, 2 hits |
(1+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry has 1 described isoform and 2 potential isoforms that are computationally mapped.Show allAlign All
10 20 30 40 50
MAGLCSSPWV KLLLAVVLSA GLPGNMANRC KKAQVKSCTE CIRVDKSCAY
60 70 80 90 100
CTDELFKERR CNTQADVLAA GCRGESVLVM ESSLEITENI QIDTSLHRSQ
110 120 130 140 150
VSPQGLQVRL RPGEERNFVF KVFEPLESPV DLYILMDFSN SMSDDLDNLK
160 170 180 190 200
QMGQNLAKIL RQLTSDYTIG FGKFVDKVSV PQTDMRPEKL KEPWPNSDPP
210 220 230 240 250
FSFKNVISLT ENVEEFWDKL QGERISGNLD APEGGFDAIL QTAVCTRDIG
260 270 280 290 300
WRADSTHLLV FSTESAFHYE ADGANVLAGI MNRNDEKCHL DATGAYTQYK
310 320 330 340 350
TQDYPSVPTL VRLLAKHNII PIFAVTNYSY SYYEKLHKYF PVSSLGVLQE
360 370 380 390 400
DSSNIVELLE EAFYRIRSNL DIRALDSPRG LRTEVTSDTL QKTETGSFHI
410 420 430 440 450
KRGEVGTYNV HLRAVEDIDG THVCQLAKED QRGNIHLKPS FSDGLRMDAS
460 470 480 490 500
VICDMCACEL QKEVQSARCH YRGDFMCGHC VCNEGWSGKT CNCSTGSLSD
510 520 530 540 550
TQPCLREGED KPCSGHGECQ CGRCVCYGEG RYEGHFCEYD NFQCPRTSGF
560 570 580 590 600
LCNDRGRCSM GECVCEPGWT GRSCDCPLSN ATCIDSNGGI CNGLGFCECG
610 620 630 640 650
RCHCNQRSSL YTDTTCEINY SAIRLGLCED LRSCVQCQAW GTGEKKGRTC
660 670 680 690 700
EECNFKVKMV DELKKAEEVV EYCSFRDEDD DCTYSYTVEG DGSPGPNSTV
710 720 730 740 750
LVHKKKDCLP APSWWLIPLL IFLLLLLVLL LLLCWKYCAC CKACLGLLPC
760 770 780 790 800
CNQGHMVGFK EDHYMLRENL MASDHLDTPM LRSGNLKGRD TVRWKITNNV
810 820 830 840 850
QRPGFATHAA SISPTELVPY GLSLRLGRLC TENLMKPGTR ECDQLRQEVE
860 870 880 890 900
ENLNEVYRQV NGVHKLQQTK FRQQPNAGKK QDHTIVDTVL LAPRSAKQSL
910 920 930 940 950
LKLTEKQVEQ GSFHELKVAP GYYTLTAEQD ARGMVEFQEG VELVDVRVPL
960 970 980 990 1000
FIRPEDDDEK QLLVEAIDVP VGTATLGRRL VNITIIKEQA SGIVSFEQPE
1010 1020 1030 1040 1050
YSVSRGDQVA RIPVIRHILD NGKSQVSYST QDNTAHGHRD YVPVEGELLF
1060 1070 1080 1090 1100
YPGETWKELQ VKLLELQEVD SLLRGRQVRR FQVQLSNPKF GARLGQPNTA
1110 1120 1130 1140 1150
TVIIGEQDET DRSLINEISA SPPLPRGDLG APQNPNAKAA GSRKIHFNWL
1160 1170 1180 1190 1200
PPPGKPMGYR VKYWVQGDSE SEAHLLDSKV PSVELTNLYP YCDYEMKVCA
1210 1220 1230 1240 1250
YGAHGEGPYS SLVSCRTHQE VPSEPGRLAF NVVSSTVTQL SWAEPAETNG
1260 1270 1280 1290 1300
EITAYEVCYG LVNEDNRPIG PMKKVLVDNP KNRMLLIENL RESQPYRYTV
1310 1320 1330 1340 1350
KARNGAGWGP EREAIINLAT QPKRPMSIPI IPDIPIVDAQ GGEDYENFLM
1360 1370 1380 1390 1400
YSDDVLRSPA SSQRPSVSDD TEHLVNGRMD FAYPGSANSL HRMTAANVAY
1410 1420 1430 1440 1450
GTHLSPHQTH RMLSTSSTLT RDYHSLTRTD HSQSGTLPRD YSTLTSLSSQ
1460 1470 1480 1490 1500
GLPPIWEDGR SRLPLSWTLG SWSRAQMKGV PASRGSPDSI ILAGQSAAPS
1510 1520 1530 1540 1550
WGTDSRGAMG VPDTPTRLVF SALGPTSLKV SWQEPQCDRA LLGYSVEYQL
1560 1570 1580 1590 1600
LNGGEMHRLN IPNPGQTSVV VEDLLPNHSY VFRVRAQSQE GWGREREGVI
1610 1620 1630 1640 1650
TIESQVHPQS PLCPLPGSAF TLSTPSAPGP LVFTALSPDS LQLSWERPRR
1660 1670 1680 1690 1700
PNGDILGYLV TCEMAQGGGP ARTFRVDGDN PESRLTVPGL SENVPYKFKV
1710 1720 1730 1740 1750
QARTTEGFGP EREGIITIES QDGGPFPQLG SHSGLFQNPL QSEYSTVTST
1760 1770 1780 1790 1800
HSTTTTEPFL IDGLTLGTQR LEAGGSLTRH VTQEFVSRTL TTSGSLSTHM
DQQFFQT
Computationally mapped potential isoform sequencesi
There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketA0A0G2K4V5 | A0A0G2K4V5_RAT | Integrin beta | Itgb4 rCG_33384 | 1,807 | Annotation score: | ||
F1LSD3 | F1LSD3_RAT | Integrin beta | Itgb4 | 1,806 | Annotation score: |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U60096 mRNA Translation: AAC53094.1 |
PIRi | JC6319 |
RefSeqi | NP_037312.1, NM_013180.1 |
Genome annotation databases
GeneIDi | 25724 |
KEGGi | rno:25724 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U60096 mRNA Translation: AAC53094.1 |
PIRi | JC6319 |
RefSeqi | NP_037312.1, NM_013180.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
6HYF | X-ray | 1.60 | A/B/C/D | 1512-1602 | [»] | |
SMRi | Q64632 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
STRINGi | 10116.ENSRNOP00000059783 |
PTM databases
GlyGeni | Q64632, 5 sites |
iPTMneti | Q64632 |
PhosphoSitePlusi | Q64632 |
Proteomic databases
PaxDbi | Q64632 |
PRIDEi | Q64632 |
Genome annotation databases
GeneIDi | 25724 |
KEGGi | rno:25724 |
Organism-specific databases
CTDi | 3691 |
RGDi | 2928, Itgb4 |
Phylogenomic databases
eggNOGi | KOG1226, Eukaryota |
InParanoidi | Q64632 |
OrthoDBi | 473040at2759 |
PhylomeDBi | Q64632 |
Enzyme and pathway databases
Reactomei | R-RNO-2022090, Assembly of collagen fibrils and other multimeric structures R-RNO-3000157, Laminin interactions R-RNO-3000170, Syndecan interactions R-RNO-446107, Type I hemidesmosome assembly |
Miscellaneous databases
PROi | PR:Q64632 |
Family and domain databases
CDDi | cd00063, FN3, 4 hits |
Gene3Di | 2.60.40.10, 4 hits 2.60.40.2030, 1 hit 3.40.50.410, 1 hit |
InterProi | View protein in InterPro IPR038081, CalX-like_sf IPR003644, Calx_beta IPR000742, EGF-like_dom IPR003961, FN3_dom IPR036116, FN3_sf IPR040622, I-EGF_1 IPR013783, Ig-like_fold IPR033760, Integrin_beta_N IPR015812, Integrin_bsu IPR012013, Integrin_bsu-4 IPR012896, Integrin_bsu_tail IPR036349, Integrin_bsu_tail_dom_sf IPR002369, Integrin_bsu_VWA IPR036465, vWFA_dom_sf |
PANTHERi | PTHR10082, PTHR10082, 3 hits PTHR10082:SF42, PTHR10082:SF42, 3 hits |
Pfami | View protein in Pfam PF03160, Calx-beta, 1 hit PF00041, fn3, 4 hits PF18372, I-EGF_1, 1 hit PF07965, Integrin_B_tail, 1 hit PF00362, Integrin_beta, 1 hit PF17205, PSI_integrin, 1 hit |
PIRSFi | PIRSF002513, Integrin_B4, 1 hit |
PRINTSi | PR01186, INTEGRINB |
SMARTi | View protein in SMART SM00237, Calx_beta, 1 hit SM00060, FN3, 4 hits SM00187, INB, 1 hit SM01242, Integrin_B_tail, 1 hit |
SUPFAMi | SSF141072, SSF141072, 1 hit SSF49265, SSF49265, 2 hits SSF53300, SSF53300, 1 hit SSF69687, SSF69687, 1 hit |
PROSITEi | View protein in PROSITE PS00022, EGF_1, 2 hits PS01186, EGF_2, 2 hits PS50853, FN3, 4 hits PS00243, INTEGRIN_BETA, 2 hits |
MobiDBi | Search... |
Entry informationi
Entry namei | ITB4_RAT | |
Accessioni | Q64632Primary (citable) accession number: Q64632 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 15, 1998 |
Last sequence update: | November 1, 1996 | |
Last modified: | February 23, 2022 | |
This is version 177 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families