UniProtKB - Q64595 (KGP2_RAT)
Protein
cGMP-dependent protein kinase 2
Gene
Prkg2
Organism
Rattus norvegicus (Rat)
Status
Functioni
Crucial regulator of intestinal secretion and bone growth (By similarity). Phosphorylates and activates CFTR on the plasma membrane (PubMed:9465038). Plays a key role in intestinal secretion by regulating cGMP-dependent translocation of CFTR in jejunum (Probable). Acts downstream of NMDAR to activate the plasma membrane accumulation of GRIA1/GLUR1 in synapse and increase synaptic plasticity. Phosphorylates GRIA1/GLUR1 at Ser-863 (PubMed:18031684). Acts as regulator of gene expression and activator of the extracellular signal-regulated kinases MAPK3/ERK1 AND MAPK1/ERK2 in mechanically stimulated osteoblasts. Under fluid shear stress, mediates ERK activation and subsequent induction of FOS, FOSL1/FRA1, FOSL2/FRA2 and FOSB that play a key role in the osteoblast anabolic response to mechanical stimulation (By similarity).By similarity1 Publication2 Publications
Catalytic activityi
Activity regulationi
Binding of cGMP results in enzyme activation.1 Publication
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 347 | cAMP or cGMP 2By similarity | 1 | |
Binding sitei | 412 | cAMP or cGMP 2By similarity | 1 | |
Binding sitei | 415 | cAMP or cGMP 2By similarity | 1 | |
Binding sitei | 482 | ATPPROSITE-ProRule annotation | 1 | |
Active sitei | 576 | Proton acceptorPROSITE-ProRule annotation | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 232 – 235 | cAMP or cGMP 1By similarity | 4 | |
Nucleotide bindingi | 242 – 243 | cAMP or cGMP 1By similarity | 2 | |
Nucleotide bindingi | 357 – 359 | cAMP or cGMP 2By similarity | 3 | |
Nucleotide bindingi | 366 – 367 | cAMP or cGMP 2By similarity | 2 | |
Nucleotide bindingi | 459 – 467 | ATPPROSITE-ProRule annotation | 9 |
GO - Molecular functioni
- ATP binding Source: UniProtKB-KW
- cGMP binding Source: UniProtKB-KW
- cGMP-dependent protein kinase activity Source: RGD
- protein homodimerization activity Source: RGD
GO - Biological processi
- negative regulation of chloride transport Source: CAFA
- peptidyl-serine autophosphorylation Source: RGD
- protein localization to plasma membrane Source: RGD
- protein phosphorylation Source: RGD
Keywordsi
Molecular function | Kinase, Serine/threonine-protein kinase, Transferase |
Ligand | ATP-binding, cGMP, cGMP-binding, Nucleotide-binding |
Enzyme and pathway databases
BRENDAi | 2.7.11.12 5301 |
Reactomei | R-RNO-1474151 Tetrahydrobiopterin (BH4) synthesis, recycling, salvage and regulation R-RNO-418457 cGMP effects |
Names & Taxonomyi
Protein namesi | Recommended name: cGMP-dependent protein kinase 2 (EC:2.7.11.121 Publication)Short name: cGK 2 Short name: cGK2 Alternative name(s): cGMP-dependent protein kinase II Short name: cGKII |
Gene namesi | Name:Prkg2 |
Organismi | Rattus norvegicus (Rat) |
Taxonomic identifieri | 10116 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Rattus |
Proteomesi |
|
Organism-specific databases
RGDi | 3401 Prkg2 |
Subcellular locationi
Plasma membrane
- Apical cell membrane By similarity; Lipid-anchor Sequence analysis
- Cell membrane 1 Publication; Lipid-anchor 1 Publication
Note: Plasma membrane localization is required for its function in the activation of CFTR.1 Publication
Nucleus
- nuclear membrane Source: RGD
Plasma Membrane
- apical plasma membrane Source: UniProtKB-SubCell
Other locations
- membrane Source: RGD
Keywords - Cellular componenti
Cell membrane, MembranePathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 2 | G → A: Cytosolic localization. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | Removed | |||
ChainiPRO_0000086125 | 2 – 762 | cGMP-dependent protein kinase 2Add BLAST | 761 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Lipidationi | 2 | N-myristoyl glycineBy similarity | 1 | |
Modified residuei | 110 | PhosphoserineCombined sources | 1 | |
Modified residuei | 117 | PhosphoserineCombined sources | 1 | |
Modified residuei | 431 | PhosphoserineCombined sources | 1 | |
Modified residuei | 609 | PhosphothreonineBy similarity | 1 |
Post-translational modificationi
Myristoylation mediates membrane localization.By similarity
Keywords - PTMi
Lipoprotein, Myristate, PhosphoproteinProteomic databases
PaxDbi | Q64595 |
PRIDEi | Q64595 |
PTM databases
iPTMneti | Q64595 |
PhosphoSitePlusi | Q64595 |
Expressioni
Tissue specificityi
Highly expressed in intestinal mucosa and is 20 times less abundant in brain and kidney (PubMed:7937783). Expressed in jejunum, in the apical domain of the villus epithelium (PubMed:15872007).2 Publications
Gene expression databases
Bgeei | ENSRNOG00000002361 Expressed in 6 organ(s), highest expression level in colon |
Genevisiblei | Q64595 RN |
Interactioni
Subunit structurei
Interacts with GRIA1/GLUR1.
1 PublicationGO - Molecular functioni
- protein homodimerization activity Source: RGD
Protein-protein interaction databases
STRINGi | 10116.ENSRNOP00000003237 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | Q64595 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 453 – 711 | Protein kinasePROSITE-ProRule annotationAdd BLAST | 259 | |
Domaini | 712 – 762 | AGC-kinase C-terminalAdd BLAST | 51 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 168 – 283 | cGMP-binding, high affinity; cAMP-binding, moderate affinityBy similarityAdd BLAST | 116 | |
Regioni | 286 – 416 | cGMP-binding, high affinity; cAMP-binding, low affinityBy similarityAdd BLAST | 131 |
Sequence similaritiesi
Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. cGMP subfamily.Curated
Phylogenomic databases
eggNOGi | KOG0616 Eukaryota ENOG410XPQQ LUCA |
GeneTreei | ENSGT00940000159393 |
HOGENOMi | HOG000233033 |
InParanoidi | Q64595 |
KOi | K19477 |
OMAi | FSGADQM |
OrthoDBi | 401933at2759 |
PhylomeDBi | Q64595 |
TreeFami | TF313261 |
Family and domain databases
CDDi | cd00038 CAP_ED, 2 hits cd05572 STKc_cGK, 1 hit |
Gene3Di | 2.60.120.10, 2 hits |
InterProi | View protein in InterPro IPR000961 AGC-kinase_C IPR002374 cGMP_dep_kinase IPR018490 cNMP-bd-like IPR018488 cNMP-bd_CS IPR000595 cNMP-bd_dom IPR011009 Kinase-like_dom_sf IPR000719 Prot_kinase_dom IPR017441 Protein_kinase_ATP_BS IPR014710 RmlC-like_jellyroll IPR008271 Ser/Thr_kinase_AS IPR035014 STKc_cGK |
Pfami | View protein in Pfam PF00027 cNMP_binding, 2 hits PF00069 Pkinase, 1 hit |
PIRSFi | PIRSF000559 cGMP-dep_kinase, 1 hit |
PRINTSi | PR00104 CGMPKINASE |
SMARTi | View protein in SMART SM00100 cNMP, 2 hits SM00133 S_TK_X, 1 hit SM00220 S_TKc, 1 hit |
SUPFAMi | SSF51206 SSF51206, 2 hits SSF56112 SSF56112, 1 hit |
PROSITEi | View protein in PROSITE PS51285 AGC_KINASE_CTER, 1 hit PS00888 CNMP_BINDING_1, 2 hits PS00889 CNMP_BINDING_2, 2 hits PS50042 CNMP_BINDING_3, 2 hits PS00107 PROTEIN_KINASE_ATP, 1 hit PS50011 PROTEIN_KINASE_DOM, 1 hit PS00108 PROTEIN_KINASE_ST, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
Q64595-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MGNGSVKPKH SKHPDGQSGN LSNEALRSKV AELEREVKRK DAELQEREYH
60 70 80 90 100
LKELREQLAK QTVAIAELTE ELQSKCIQLN KLQDVIHVQG GSPLQASPDK
110 120 130 140 150
VPLDVHRKTS GLVSLHSRRG AKAGVSAEPT SRTYDLNKPP EFSFEKARVR
160 170 180 190 200
KDSSEKKLIT DALNKNQFLK RLDPQQIKDM VECMYGRNYQ QGSYIVKQGE
210 220 230 240 250
PGNHIFVLAE GRLEVFQGEK LLSSIPMWTT FGELAILYNC TRTASVKAIT
260 270 280 290 300
NVKTWALDRE VFQNIMRRTA QARDEEYRNF LRSVSLLKNL PEDKLTKIID
310 320 330 340 350
CLEVEYYDKG DYIIREGEEG STFFILAKGK VKVTQSTEGH DQPQLIKTLQ
360 370 380 390 400
KGEYFGEKAL ISDDVRSANI IAEENDVACL VIDRETFNQT VGTFDELQKY
410 420 430 440 450
LEGYVATLNR DDEKRHAKRS MSSWKLSKAL SLEMIQLKEK VARFSSTSPF
460 470 480 490 500
QNLEIIATLG VGGFGRVELV KVKNENIAFA MKCIRKKHIV DTKQQEHVYS
510 520 530 540 550
EKRILEELCS PFIVKLYRTF KDNKYVYMLL EACLGGELWS ILRDRGSFDE
560 570 580 590 600
PTSKFCVACV TEAFDYLHRL GIIYRDLKPE NLILDADGYL KLVDFGFAKK
610 620 630 640 650
IGSGQKTWTF CGTPEYVAPE VILNKGHDFS VDFWSLGILV YELLTGNPPF
660 670 680 690 700
SGIDQMMTYN LILKGIEKMD FPRKITRRPE DLIRRLCRQN PTERLGNLKN
710 720 730 740 750
GINDIKKHRW LNGFNWEGLK ARSLPSPLRR ELSGPIDHSY FDKYPPEKGV
760
PPDEMSGWDK DF
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Z36276 mRNA Translation: CAA85284.1 |
PIRi | I59329 |
RefSeqi | NP_037144.1, NM_013012.1 |
Genome annotation databases
Ensembli | ENSRNOT00000003237; ENSRNOP00000003237; ENSRNOG00000002361 |
GeneIDi | 25523 |
KEGGi | rno:25523 |
UCSCi | RGD:3401 rat |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Z36276 mRNA Translation: CAA85284.1 |
PIRi | I59329 |
RefSeqi | NP_037144.1, NM_013012.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
4OJK | X-ray | 2.66 | C/D | 40-83 | [»] | |
SMRi | Q64595 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
STRINGi | 10116.ENSRNOP00000003237 |
PTM databases
iPTMneti | Q64595 |
PhosphoSitePlusi | Q64595 |
Proteomic databases
PaxDbi | Q64595 |
PRIDEi | Q64595 |
Genome annotation databases
Ensembli | ENSRNOT00000003237; ENSRNOP00000003237; ENSRNOG00000002361 |
GeneIDi | 25523 |
KEGGi | rno:25523 |
UCSCi | RGD:3401 rat |
Organism-specific databases
CTDi | 5593 |
RGDi | 3401 Prkg2 |
Phylogenomic databases
eggNOGi | KOG0616 Eukaryota ENOG410XPQQ LUCA |
GeneTreei | ENSGT00940000159393 |
HOGENOMi | HOG000233033 |
InParanoidi | Q64595 |
KOi | K19477 |
OMAi | FSGADQM |
OrthoDBi | 401933at2759 |
PhylomeDBi | Q64595 |
TreeFami | TF313261 |
Enzyme and pathway databases
BRENDAi | 2.7.11.12 5301 |
Reactomei | R-RNO-1474151 Tetrahydrobiopterin (BH4) synthesis, recycling, salvage and regulation R-RNO-418457 cGMP effects |
Miscellaneous databases
PROi | PR:Q64595 |
Gene expression databases
Bgeei | ENSRNOG00000002361 Expressed in 6 organ(s), highest expression level in colon |
Genevisiblei | Q64595 RN |
Family and domain databases
CDDi | cd00038 CAP_ED, 2 hits cd05572 STKc_cGK, 1 hit |
Gene3Di | 2.60.120.10, 2 hits |
InterProi | View protein in InterPro IPR000961 AGC-kinase_C IPR002374 cGMP_dep_kinase IPR018490 cNMP-bd-like IPR018488 cNMP-bd_CS IPR000595 cNMP-bd_dom IPR011009 Kinase-like_dom_sf IPR000719 Prot_kinase_dom IPR017441 Protein_kinase_ATP_BS IPR014710 RmlC-like_jellyroll IPR008271 Ser/Thr_kinase_AS IPR035014 STKc_cGK |
Pfami | View protein in Pfam PF00027 cNMP_binding, 2 hits PF00069 Pkinase, 1 hit |
PIRSFi | PIRSF000559 cGMP-dep_kinase, 1 hit |
PRINTSi | PR00104 CGMPKINASE |
SMARTi | View protein in SMART SM00100 cNMP, 2 hits SM00133 S_TK_X, 1 hit SM00220 S_TKc, 1 hit |
SUPFAMi | SSF51206 SSF51206, 2 hits SSF56112 SSF56112, 1 hit |
PROSITEi | View protein in PROSITE PS51285 AGC_KINASE_CTER, 1 hit PS00888 CNMP_BINDING_1, 2 hits PS00889 CNMP_BINDING_2, 2 hits PS50042 CNMP_BINDING_3, 2 hits PS00107 PROTEIN_KINASE_ATP, 1 hit PS50011 PROTEIN_KINASE_DOM, 1 hit PS00108 PROTEIN_KINASE_ST, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | KGP2_RAT | |
Accessioni | Q64595Primary (citable) accession number: Q64595 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | May 30, 2000 |
Last sequence update: | November 1, 1996 | |
Last modified: | October 16, 2019 | |
This is version 148 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Complete proteome, Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families - PDB cross-references
Index of Protein Data Bank (PDB) cross-references