UniProtKB - Q64595 (KGP2_RAT)
cGMP-dependent protein kinase 2
Prkg2
Functioni
Crucial regulator of intestinal secretion and bone growth (By similarity).
Phosphorylates and activates CFTR on the plasma membrane (PubMed:9465038).
Plays a key role in intestinal secretion by regulating cGMP-dependent translocation of CFTR in jejunum (Probable). Acts downstream of NMDAR to activate the plasma membrane accumulation of GRIA1/GLUR1 in synapse and increase synaptic plasticity. Phosphorylates GRIA1/GLUR1 at Ser-863 (PubMed:18031684).
Acts as regulator of gene expression and activator of the extracellular signal-regulated kinases MAPK3/ERK1 AND MAPK1/ERK2 in mechanically stimulated osteoblasts. Under fluid shear stress, mediates ERK activation and subsequent induction of FOS, FOSL1/FRA1, FOSL2/FRA2 and FOSB that play a key role in the osteoblast anabolic response to mechanical stimulation (By similarity).
By similarity1 Publication2 PublicationsCatalytic activityi
Activity regulationi
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 347 | 3',5'-cGMP 2By similarity | 1 | |
Binding sitei | 412 | 3',5'-cGMP 2By similarity | 1 | |
Binding sitei | 415 | 3',5'-cGMP 2By similarity | 1 | |
Binding sitei | 482 | ATPPROSITE-ProRule annotation | 1 | |
Active sitei | 576 | Proton acceptorPROSITE-ProRule annotation | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 232 – 235 | 3',5'-cGMP 1By similarity | 4 | |
Nucleotide bindingi | 242 – 243 | 3',5'-cGMP 1By similarity | 2 | |
Nucleotide bindingi | 356 – 359 | 3',5'-cGMP 2By similarity | 4 | |
Nucleotide bindingi | 366 – 367 | 3',5'-cGMP 2By similarity | 2 | |
Nucleotide bindingi | 459 – 467 | ATPPROSITE-ProRule annotation | 9 |
GO - Molecular functioni
- ATP binding Source: UniProtKB-KW
- cGMP binding Source: UniProtKB-KW
- cGMP-dependent protein kinase activity Source: RGD
- identical protein binding Source: RGD
- protein serine/threonine/tyrosine kinase activity Source: RHEA
- protein serine kinase activity Source: RHEA
GO - Biological processi
- cellular hypotonic response Source: RGD
- circadian regulation of gene expression Source: RGD
- circadian rhythm Source: RGD
- negative regulation of cell volume Source: RGD
- negative regulation of chloride transport Source: CAFA
- peptidyl-serine autophosphorylation Source: RGD
- protein localization to plasma membrane Source: RGD
- protein phosphorylation Source: RGD
Keywordsi
Molecular function | Kinase, Serine/threonine-protein kinase, Transferase |
Ligand | ATP-binding, cGMP, cGMP-binding, Nucleotide-binding |
Enzyme and pathway databases
BRENDAi | 2.7.11.12, 5301 |
Reactomei | R-RNO-1474151, Tetrahydrobiopterin (BH4) synthesis, recycling, salvage and regulation R-RNO-418457, cGMP effects R-RNO-9648002, RAS processing |
Names & Taxonomyi
Protein namesi | Recommended name: cGMP-dependent protein kinase 2 (EC:2.7.11.121 Publication)Short name: cGK 2 Short name: cGK2 Alternative name(s): cGMP-dependent protein kinase II Short name: cGKII |
Gene namesi | Name:Prkg2 |
Organismi | Rattus norvegicus (Rat) |
Taxonomic identifieri | 10116 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Rattus |
Proteomesi |
|
Organism-specific databases
RGDi | 3401, Prkg2 |
Subcellular locationi
Plasma membrane
- Apical cell membrane By similarity; Lipid-anchor Sequence analysis
- Cell membrane 1 Publication; Lipid-anchor 1 Publication
Note: Plasma membrane localization is required for its function in the activation of CFTR.1 Publication
Nucleus
- nuclear membrane Source: RGD
Plasma Membrane
- apical plasma membrane Source: UniProtKB-SubCell
- plasma membrane Source: RGD
Other locations
- membrane Source: RGD
Keywords - Cellular componenti
Cell membrane, MembranePathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 2 | G → A: Cytosolic localization. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | Removed | |||
ChainiPRO_0000086125 | 2 – 762 | cGMP-dependent protein kinase 2Add BLAST | 761 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Lipidationi | 2 | N-myristoyl glycineBy similarity | 1 | |
Modified residuei | 110 | PhosphoserineCombined sources | 1 | |
Modified residuei | 117 | PhosphoserineCombined sources | 1 | |
Modified residuei | 431 | PhosphoserineCombined sources | 1 | |
Modified residuei | 609 | PhosphothreonineBy similarity | 1 |
Post-translational modificationi
Keywords - PTMi
Lipoprotein, Myristate, PhosphoproteinProteomic databases
PaxDbi | Q64595 |
PTM databases
iPTMneti | Q64595 |
PhosphoSitePlusi | Q64595 |
Expressioni
Tissue specificityi
Gene expression databases
Bgeei | ENSRNOG00000002361, Expressed in jejunum and 16 other tissues |
Genevisiblei | Q64595, RN |
Interactioni
Subunit structurei
Interacts with GRIA1/GLUR1.
1 PublicationGO - Molecular functioni
- identical protein binding Source: RGD
Protein-protein interaction databases
STRINGi | 10116.ENSRNOP00000003237 |
Structurei
Secondary structure
3D structure databases
SMRi | Q64595 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 453 – 711 | Protein kinasePROSITE-ProRule annotationAdd BLAST | 259 | |
Domaini | 712 – 762 | AGC-kinase C-terminalPROSITE-ProRule annotationAdd BLAST | 51 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 25 | DisorderedSequence analysisAdd BLAST | 25 | |
Regioni | 112 – 138 | DisorderedSequence analysisAdd BLAST | 27 | |
Regioni | 168 – 283 | cGMP-binding, high affinity; cAMP-binding, moderate affinityBy similarityAdd BLAST | 116 | |
Regioni | 286 – 416 | cGMP-binding, high affinity; cAMP-binding, low affinityBy similarityAdd BLAST | 131 | |
Regioni | 740 – 762 | DisorderedSequence analysisAdd BLAST | 23 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG0614, Eukaryota |
GeneTreei | ENSGT00940000159393 |
HOGENOMi | CLU_000288_73_2_1 |
InParanoidi | Q64595 |
OMAi | SKNPDGH |
OrthoDBi | 401933at2759 |
PhylomeDBi | Q64595 |
TreeFami | TF313261 |
Family and domain databases
CDDi | cd00038, CAP_ED, 2 hits cd05572, STKc_cGK, 1 hit |
Gene3Di | 2.60.120.10, 2 hits |
InterProi | View protein in InterPro IPR000961, AGC-kinase_C IPR002374, cGMP_dep_kinase IPR018490, cNMP-bd-like IPR018488, cNMP-bd_CS IPR000595, cNMP-bd_dom IPR011009, Kinase-like_dom_sf IPR000719, Prot_kinase_dom IPR017441, Protein_kinase_ATP_BS IPR014710, RmlC-like_jellyroll IPR008271, Ser/Thr_kinase_AS IPR035014, STKc_cGK |
Pfami | View protein in Pfam PF00027, cNMP_binding, 2 hits PF00069, Pkinase, 1 hit |
PIRSFi | PIRSF000559, cGMP-dep_kinase, 1 hit |
PRINTSi | PR00104, CGMPKINASE |
SMARTi | View protein in SMART SM00100, cNMP, 2 hits SM00133, S_TK_X, 1 hit SM00220, S_TKc, 1 hit |
SUPFAMi | SSF51206, SSF51206, 2 hits SSF56112, SSF56112, 1 hit |
PROSITEi | View protein in PROSITE PS51285, AGC_KINASE_CTER, 1 hit PS00888, CNMP_BINDING_1, 2 hits PS00889, CNMP_BINDING_2, 2 hits PS50042, CNMP_BINDING_3, 2 hits PS00107, PROTEIN_KINASE_ATP, 1 hit PS50011, PROTEIN_KINASE_DOM, 1 hit PS00108, PROTEIN_KINASE_ST, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
10 20 30 40 50
MGNGSVKPKH SKHPDGQSGN LSNEALRSKV AELEREVKRK DAELQEREYH
60 70 80 90 100
LKELREQLAK QTVAIAELTE ELQSKCIQLN KLQDVIHVQG GSPLQASPDK
110 120 130 140 150
VPLDVHRKTS GLVSLHSRRG AKAGVSAEPT SRTYDLNKPP EFSFEKARVR
160 170 180 190 200
KDSSEKKLIT DALNKNQFLK RLDPQQIKDM VECMYGRNYQ QGSYIVKQGE
210 220 230 240 250
PGNHIFVLAE GRLEVFQGEK LLSSIPMWTT FGELAILYNC TRTASVKAIT
260 270 280 290 300
NVKTWALDRE VFQNIMRRTA QARDEEYRNF LRSVSLLKNL PEDKLTKIID
310 320 330 340 350
CLEVEYYDKG DYIIREGEEG STFFILAKGK VKVTQSTEGH DQPQLIKTLQ
360 370 380 390 400
KGEYFGEKAL ISDDVRSANI IAEENDVACL VIDRETFNQT VGTFDELQKY
410 420 430 440 450
LEGYVATLNR DDEKRHAKRS MSSWKLSKAL SLEMIQLKEK VARFSSTSPF
460 470 480 490 500
QNLEIIATLG VGGFGRVELV KVKNENIAFA MKCIRKKHIV DTKQQEHVYS
510 520 530 540 550
EKRILEELCS PFIVKLYRTF KDNKYVYMLL EACLGGELWS ILRDRGSFDE
560 570 580 590 600
PTSKFCVACV TEAFDYLHRL GIIYRDLKPE NLILDADGYL KLVDFGFAKK
610 620 630 640 650
IGSGQKTWTF CGTPEYVAPE VILNKGHDFS VDFWSLGILV YELLTGNPPF
660 670 680 690 700
SGIDQMMTYN LILKGIEKMD FPRKITRRPE DLIRRLCRQN PTERLGNLKN
710 720 730 740 750
GINDIKKHRW LNGFNWEGLK ARSLPSPLRR ELSGPIDHSY FDKYPPEKGV
760
PPDEMSGWDK DF
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Z36276 mRNA Translation: CAA85284.1 |
PIRi | I59329 |
RefSeqi | NP_037144.1, NM_013012.1 |
Genome annotation databases
Ensembli | ENSRNOT00000003237; ENSRNOP00000003237; ENSRNOG00000002361 |
GeneIDi | 25523 |
KEGGi | rno:25523 |
UCSCi | RGD:3401, rat |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Z36276 mRNA Translation: CAA85284.1 |
PIRi | I59329 |
RefSeqi | NP_037144.1, NM_013012.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
4OJK | X-ray | 2.66 | C/D | 40-83 | [»] | |
SMRi | Q64595 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
STRINGi | 10116.ENSRNOP00000003237 |
PTM databases
iPTMneti | Q64595 |
PhosphoSitePlusi | Q64595 |
Proteomic databases
PaxDbi | Q64595 |
Genome annotation databases
Ensembli | ENSRNOT00000003237; ENSRNOP00000003237; ENSRNOG00000002361 |
GeneIDi | 25523 |
KEGGi | rno:25523 |
UCSCi | RGD:3401, rat |
Organism-specific databases
CTDi | 5593 |
RGDi | 3401, Prkg2 |
Phylogenomic databases
eggNOGi | KOG0614, Eukaryota |
GeneTreei | ENSGT00940000159393 |
HOGENOMi | CLU_000288_73_2_1 |
InParanoidi | Q64595 |
OMAi | SKNPDGH |
OrthoDBi | 401933at2759 |
PhylomeDBi | Q64595 |
TreeFami | TF313261 |
Enzyme and pathway databases
BRENDAi | 2.7.11.12, 5301 |
Reactomei | R-RNO-1474151, Tetrahydrobiopterin (BH4) synthesis, recycling, salvage and regulation R-RNO-418457, cGMP effects R-RNO-9648002, RAS processing |
Miscellaneous databases
PROi | PR:Q64595 |
Gene expression databases
Bgeei | ENSRNOG00000002361, Expressed in jejunum and 16 other tissues |
Genevisiblei | Q64595, RN |
Family and domain databases
CDDi | cd00038, CAP_ED, 2 hits cd05572, STKc_cGK, 1 hit |
Gene3Di | 2.60.120.10, 2 hits |
InterProi | View protein in InterPro IPR000961, AGC-kinase_C IPR002374, cGMP_dep_kinase IPR018490, cNMP-bd-like IPR018488, cNMP-bd_CS IPR000595, cNMP-bd_dom IPR011009, Kinase-like_dom_sf IPR000719, Prot_kinase_dom IPR017441, Protein_kinase_ATP_BS IPR014710, RmlC-like_jellyroll IPR008271, Ser/Thr_kinase_AS IPR035014, STKc_cGK |
Pfami | View protein in Pfam PF00027, cNMP_binding, 2 hits PF00069, Pkinase, 1 hit |
PIRSFi | PIRSF000559, cGMP-dep_kinase, 1 hit |
PRINTSi | PR00104, CGMPKINASE |
SMARTi | View protein in SMART SM00100, cNMP, 2 hits SM00133, S_TK_X, 1 hit SM00220, S_TKc, 1 hit |
SUPFAMi | SSF51206, SSF51206, 2 hits SSF56112, SSF56112, 1 hit |
PROSITEi | View protein in PROSITE PS51285, AGC_KINASE_CTER, 1 hit PS00888, CNMP_BINDING_1, 2 hits PS00889, CNMP_BINDING_2, 2 hits PS50042, CNMP_BINDING_3, 2 hits PS00107, PROTEIN_KINASE_ATP, 1 hit PS50011, PROTEIN_KINASE_DOM, 1 hit PS00108, PROTEIN_KINASE_ST, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | KGP2_RAT | |
Accessioni | Q64595Primary (citable) accession number: Q64595 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | May 30, 2000 |
Last sequence update: | November 1, 1996 | |
Last modified: | February 23, 2022 | |
This is version 158 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families