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Protein

Plasma membrane calcium-transporting ATPase 3

Gene

Atp2b3

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the transport of calcium out of the cell.

Catalytic activityi

ATP + H2O + Ca2+(Side 1) = ADP + phosphate + Ca2+(Side 2).

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei4734-aspartylphosphate intermediateBy similarity1
Metal bindingi794MagnesiumBy similarity1
Metal bindingi798MagnesiumBy similarity1

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • calcium-transporting ATPase activity Source: GO_Central
  • calmodulin binding Source: UniProtKB-KW
  • metal ion binding Source: UniProtKB-KW
  • PDZ domain binding Source: RGD

GO - Biological processi

Keywordsi

Molecular functionCalmodulin-binding, Hydrolase
Biological processCalcium transport, Ion transport, Transport
LigandATP-binding, Calcium, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-RNO-418359 Reduction of cytosolic Ca++ levels
R-RNO-5578775 Ion homeostasis
R-RNO-936837 Ion transport by P-type ATPases

Names & Taxonomyi

Protein namesi
Recommended name:
Plasma membrane calcium-transporting ATPase 3 (EC:3.6.3.8)
Short name:
PMCA3
Alternative name(s):
Plasma membrane calcium ATPase isoform 3
Plasma membrane calcium pump isoform 3
Gene namesi
Name:Atp2b3
Synonyms:Pmca3
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome X

Organism-specific databases

RGDi621304 Atp2b3

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 97CytoplasmicSequence analysisAdd BLAST97
Transmembranei98 – 118HelicalSequence analysisAdd BLAST21
Topological domaini119 – 155ExtracellularSequence analysisAdd BLAST37
Transmembranei156 – 176HelicalSequence analysisAdd BLAST21
Topological domaini177 – 364CytoplasmicSequence analysisAdd BLAST188
Transmembranei365 – 384HelicalSequence analysisAdd BLAST20
Topological domaini385 – 417ExtracellularSequence analysisAdd BLAST33
Transmembranei418 – 435HelicalSequence analysisAdd BLAST18
Topological domaini436 – 849CytoplasmicSequence analysisAdd BLAST414
Transmembranei850 – 869HelicalSequence analysisAdd BLAST20
Topological domaini870 – 879ExtracellularSequence analysis10
Transmembranei880 – 900HelicalSequence analysisAdd BLAST21
Topological domaini901 – 920CytoplasmicSequence analysisAdd BLAST20
Transmembranei921 – 943HelicalSequence analysisAdd BLAST23
Topological domaini944 – 961ExtracellularSequence analysisAdd BLAST18
Transmembranei962 – 983HelicalSequence analysisAdd BLAST22
Topological domaini984 – 1002CytoplasmicSequence analysisAdd BLAST19
Transmembranei1003 – 1024HelicalSequence analysisAdd BLAST22
Topological domaini1025 – 1034ExtracellularSequence analysis10
Transmembranei1035 – 1056HelicalSequence analysisAdd BLAST22
Topological domaini1057 – 1258CytoplasmicSequence analysisAdd BLAST202

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000462191 – 1258Plasma membrane calcium-transporting ATPase 3Add BLAST1258

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei8PhosphoserineCombined sources1
Modified residuei1079PhosphothreonineCombined sources1
Modified residuei1113Phosphothreonine; by PKCBy similarity1
Modified residuei1126PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ64568
PRIDEiQ64568

PTM databases

iPTMnetiQ64568
PhosphoSitePlusiQ64568
SwissPalmiQ64568

Expressioni

Tissue specificityi

Isoform XA is the most abundant form in brain and most other tissues. Isoform XB is the most abundant form in skeletal muscle and is also found in brain and at low levels in testis and kidney. The other isoforms are only found at low levels in various tissues.

Gene expression databases

BgeeiENSRNOG00000061304 Expressed in 7 organ(s), highest expression level in brain
ExpressionAtlasiQ64568 baseline and differential

Interactioni

Subunit structurei

Interacts with PDZD11.By similarity

GO - Molecular functioni

Protein-protein interaction databases

BioGridi248231, 1 interactor
STRINGi10116.ENSRNOP00000035695

Structurei

3D structure databases

ProteinModelPortaliQ64568
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1097 – 1114Calmodulin-binding subdomain ABy similarityAdd BLAST18
Regioni1115 – 1124Calmodulin-binding subdomain BBy similarity10

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi297 – 300Poly-Glu4
Compositional biasi1212 – 1217Poly-Pro6

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0204 Eukaryota
ENOG410XNNC LUCA
GeneTreeiENSGT00510000046331
HOGENOMiHOG000265623
HOVERGENiHBG061286
InParanoidiQ64568
KOiK05850
PhylomeDBiQ64568

Family and domain databases

Gene3Di3.40.1110.10, 1 hit
3.40.50.1000, 1 hit
InterProiView protein in InterPro
IPR030325 ATP2B3
IPR022141 ATP_Ca_trans_C
IPR006068 ATPase_P-typ_cation-transptr_C
IPR004014 ATPase_P-typ_cation-transptr_N
IPR023299 ATPase_P-typ_cyto_dom_N
IPR018303 ATPase_P-typ_P_site
IPR023298 ATPase_P-typ_TM_dom_sf
IPR008250 ATPase_P-typ_transduc_dom_A_sf
IPR036412 HAD-like_sf
IPR023214 HAD_sf
IPR006408 P-type_ATPase_IIB
IPR001757 P_typ_ATPase
PANTHERiPTHR24093:SF284 PTHR24093:SF284, 1 hit
PfamiView protein in Pfam
PF12424 ATP_Ca_trans_C, 2 hits
PF00689 Cation_ATPase_C, 1 hit
PF00690 Cation_ATPase_N, 1 hit
SMARTiView protein in SMART
SM00831 Cation_ATPase_N, 1 hit
SUPFAMiSSF56784 SSF56784, 1 hit
SSF81653 SSF81653, 1 hit
SSF81660 SSF81660, 1 hit
SSF81665 SSF81665, 1 hit
TIGRFAMsiTIGR01517 ATPase-IIB_Ca, 1 hit
TIGR01494 ATPase_P-type, 3 hits
PROSITEiView protein in PROSITE
PS00154 ATPASE_E1_E2, 1 hit

Sequences (12+)i

Sequence statusi: Complete.

This entry describes 12 isoformsi produced by alternative splicing. AlignAdd to basket
Note: There is a combination of two alternative spliced domains at N-terminal site A (X and Z) and at C-terminal site C (A, B, C, D, E and F). So far the splice sites have been studied independently. Experimental confirmation may be lacking for some isoforms.

This entry has 12 described isoforms and 1 potential isoform that is computationally mapped.Show allAlign All

Isoform XD (identifier: Q64568-1) [UniParc]FASTAAdd to basket
Also known as: AIICIV

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MGDMANSSIE FHPKPQQQRE VPHVGGFGCT LAELRSLMEL RGAEALQKIQ
60 70 80 90 100
EAYGDVSGLC RRLKTSPTEG LADNTNDLEK RRQIYGQNFI PPKQPKTFLQ
110 120 130 140 150
LVWEALQDVT LIILEVAAIV SLGLSFYAPP GEESEACGNV SGGAEDEGEA
160 170 180 190 200
EAGWIEGAAI LLSVICVVLV TAFNDWSKEK QFRGLQSRIE QEQKFTVIRN
210 220 230 240 250
GQLLQVPVAA LVVGDIAQVK YGDLLPADGV LIQGNDLKID ESSLTGESDH
260 270 280 290 300
VRKSADKDPM LLSGTHVMEG SGRMVVTAVG VNSQTGIIFT LLGAGGEEEE
310 320 330 340 350
KKDKKGKQQD GAMESSQTKA KKQDGAVAME MQPLKSAEGG EMEEREKKKA
360 370 380 390 400
NVPKKEKSVL QGKLTKLAVQ IGKAGLVMSA ITVIILVLYF VIETFVVDGR
410 420 430 440 450
VWLAECTPVY VQYFVKFFII GVTVLVVAVP EGLPLAVTIS LAYSVKKMMK
460 470 480 490 500
DNNLVRHLDA CETMGNATAI CSDKTGTLTT NRMTVVQSYL GDTHYKEIPA
510 520 530 540 550
PSALTPKILD LLVHAISINS AYTTKILPPE KEGALPRQVG NKTECALLGF
560 570 580 590 600
ILDLKRDFQP VREQIPEDQL YKVYTFNSVR KSMSTVIRMP DGGFRLFSKG
610 620 630 640 650
ASEILLKKCT NILNSNGELR GFRPRDRDDM VKKIIEPMAC DGLRTICIAY
660 670 680 690 700
RDFSAIQEPD WDNENEVVGD LTCIAVVGIE DPVRPEVPEA IRKCQRAGIT
710 720 730 740 750
VRMVTGDNIN TARAIAAKCG IIQPGEDFLC LEGKEFNRRI RNEKGEIEQE
760 770 780 790 800
RLDKVWPKLR VLARSSPTDK HTLVKGIIDS TTGEQRQVVA VTGDGTNDGP
810 820 830 840 850
ALKKADVGFA MGIAGTDVAK EASDIILTDD NFTSIVKAVM WGRNVYDSIS
860 870 880 890 900
KFLQFQLTVN VVAVIVAFTG ACITQDSPLK AVQMLWVNLI MDTFASLALA
910 920 930 940 950
TEPPTESLLL RKPYGRDKPL ISRTMMKNIL GHAVYQLTII FTLLFVGELF
960 970 980 990 1000
FDIDSGRNAP LHSPPSEHYT IIFNTFVMMQ LFNEINARKI HGERNVFDGI
1010 1020 1030 1040 1050
FSNPIFCTIV LGTFGIQIVI VQFGGKPFSC SPLSTEQWLW CLFVGVGELV
1060 1070 1080 1090 1100
WGQVIATIPT SQLKCLKEAG HGPGKDEMTD EELAEGEEEI DHAERELRRG
1110 1120 1130 1140 1150
QILWFRGLNR IQTQMEVVST FKRSGSFQGA VRRRSSVLSQ LHDVTNLSTP
1160 1170 1180 1190 1200
THIRVVKAFR SSLYEGLEKP ESKSCIHNFM ATPEFLINDY THNIPLIDDT
1210 1220 1230 1240 1250
DVDENEERLR APPPPPPNQN NNAIDSGIYL TTHATKSATS SAFSSRPGSP

LHSMETSL
Length:1,258
Mass (Da):138,559
Last modified:June 1, 2001 - v2
Checksum:i3A13781D21042BA6
GO
Isoform XA (identifier: Q64568-2) [UniParc]FASTAAdd to basket
Also known as: AIICII

The sequence of this isoform differs from the canonical sequence as follows:
     1153-1258: IRVVKAFRSS...SPLHSMETSL → VTLSAAKPTSAAGNPSGESIP

Show »
Length:1,173
Mass (Da):128,773
Checksum:iCF49538EC579E958
GO
Isoform ZA (identifier: Q64568-3) [UniParc]FASTAAdd to basket
Also known as: AICII

The sequence of this isoform differs from the canonical sequence as follows:
     306-319: Missing.
     1153-1258: IRVVKAFRSS...SPLHSMETSL → VTLSAAKPTSAAGNPSGESIP

Show »
Length:1,159
Mass (Da):127,297
Checksum:iA33A52545101241D
GO
Isoform XB (identifier: Q64568-4) [UniParc]FASTAAdd to basket
Also known as: AIICI

The sequence of this isoform differs from the canonical sequence as follows:
     1115-1152: Missing.

Show »
Length:1,220
Mass (Da):134,360
Checksum:iC18A4BAEBA0362ED
GO
Isoform ZB (identifier: Q64568-5) [UniParc]FASTAAdd to basket
Also known as: AICI

The sequence of this isoform differs from the canonical sequence as follows:
     306-319: Missing.
     1115-1152: Missing.

Show »
Length:1,206
Mass (Da):132,884
Checksum:iF83F2E1301CB4552
GO
Isoform XC (identifier: Q64568-6) [UniParc]FASTAAdd to basket
Also known as: AIICIII

The sequence of this isoform differs from the canonical sequence as follows:
     1144-1152: Missing.

Show »
Length:1,249
Mass (Da):137,608
Checksum:i9825517A8B9744B1
GO
Isoform ZC (identifier: Q64568-7) [UniParc]FASTAAdd to basket
Also known as: AICIII

The sequence of this isoform differs from the canonical sequence as follows:
     306-319: Missing.
     1144-1152: Missing.

Show »
Length:1,235
Mass (Da):136,132
Checksum:iDD7B43D805121FDD
GO
Isoform ZD (identifier: Q64568-8) [UniParc]FASTAAdd to basket
Also known as: AICIV

The sequence of this isoform differs from the canonical sequence as follows:
     306-319: Missing.

Show »
Length:1,244
Mass (Da):137,083
Checksum:i3BA47725ACB4D4AE
GO
Isoform XE (identifier: Q64568-9) [UniParc]FASTAAdd to basket
Also known as: AIICV

The sequence of this isoform differs from the canonical sequence as follows:
     1153-1258: IRVVKAFRSS...SPLHSMETSL → VTLSAAKPTSAAGSES

Show »
Length:1,168
Mass (Da):128,295
Checksum:i09F539391396F9A2
GO
Isoform ZE (identifier: Q64568-10) [UniParc]FASTAAdd to basket
Also known as: AICV

The sequence of this isoform differs from the canonical sequence as follows:
     306-319: Missing.
     1153-1258: IRVVKAFRSS...SPLHSMETSL → VTLSAAKPTSAAGSES

Show »
Length:1,154
Mass (Da):126,818
Checksum:i81387C79448BCC0F
GO
Isoform XF (identifier: Q64568-11) [UniParc]FASTAAdd to basket
Also known as: AIICVI

The sequence of this isoform differs from the canonical sequence as follows:
     1115-1258: MEVVSTFKRS...SPLHSMETSL → VCWDGKKMLRTTEVG

Show »
Length:1,129
Mass (Da):124,342
Checksum:i7B4F423A0C709026
GO
Isoform ZF (identifier: Q64568-12) [UniParc]FASTAAdd to basket
Also known as: AICVI

The sequence of this isoform differs from the canonical sequence as follows:
     306-319: Missing.
     1115-1258: MEVVSTFKRS...SPLHSMETSL → VCWDGKKMLRTTEVG

Show »
Length:1,115
Mass (Da):122,866
Checksum:i1A545F41D1A951EB
GO

Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
A0A0G2K9Q6A0A0G2K9Q6_RAT
Calcium-transporting ATPase
Atp2b3
1,258Annotation score:

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_000396306 – 319Missing in isoform ZA, isoform ZB, isoform ZC, isoform ZD, isoform ZE and isoform ZF. 1 PublicationAdd BLAST14
Alternative sequenceiVSP_0003981115 – 1258MEVVS…METSL → VCWDGKKMLRTTEVG in isoform XF and isoform ZF. CuratedAdd BLAST144
Alternative sequenceiVSP_0003971115 – 1152Missing in isoform XB and isoform ZB. CuratedAdd BLAST38
Alternative sequenceiVSP_0003991144 – 1152Missing in isoform XC and isoform ZC. Curated9
Alternative sequenceiVSP_0004001153 – 1258IRVVK…METSL → VTLSAAKPTSAAGNPSGESI P in isoform XA and isoform ZA. 1 PublicationAdd BLAST106
Alternative sequenceiVSP_0004011153 – 1258IRVVK…METSL → VTLSAAKPTSAAGSES in isoform XE and isoform ZE. CuratedAdd BLAST106

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L05565, L05558, L05564 Genomic DNA Translation: AAA53650.1
J05087 mRNA Translation: AAA69667.1
M96626 mRNA Translation: AAA50821.1
PIRiA34308
C44525
RefSeqiNP_579822.1, NM_133288.1 [Q64568-3]
XP_008771842.1, XM_008773620.2 [Q64568-7]
XP_017457435.1, XM_017601946.1 [Q64568-5]
XP_017457436.1, XM_017601947.1 [Q64568-3]
XP_017457437.1, XM_017601948.1 [Q64568-3]
UniGeneiRn.11053

Genome annotation databases

EnsembliENSRNOT00000077979; ENSRNOP00000071471; ENSRNOG00000061304 [Q64568-3]
GeneIDi29599
KEGGirno:29599

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L05565, L05558, L05564 Genomic DNA Translation: AAA53650.1
J05087 mRNA Translation: AAA69667.1
M96626 mRNA Translation: AAA50821.1
PIRiA34308
C44525
RefSeqiNP_579822.1, NM_133288.1 [Q64568-3]
XP_008771842.1, XM_008773620.2 [Q64568-7]
XP_017457435.1, XM_017601946.1 [Q64568-5]
XP_017457436.1, XM_017601947.1 [Q64568-3]
XP_017457437.1, XM_017601948.1 [Q64568-3]
UniGeneiRn.11053

3D structure databases

ProteinModelPortaliQ64568
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi248231, 1 interactor
STRINGi10116.ENSRNOP00000035695

PTM databases

iPTMnetiQ64568
PhosphoSitePlusiQ64568
SwissPalmiQ64568

Proteomic databases

PaxDbiQ64568
PRIDEiQ64568

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000077979; ENSRNOP00000071471; ENSRNOG00000061304 [Q64568-3]
GeneIDi29599
KEGGirno:29599

Organism-specific databases

CTDi492
RGDi621304 Atp2b3

Phylogenomic databases

eggNOGiKOG0204 Eukaryota
ENOG410XNNC LUCA
GeneTreeiENSGT00510000046331
HOGENOMiHOG000265623
HOVERGENiHBG061286
InParanoidiQ64568
KOiK05850
PhylomeDBiQ64568

Enzyme and pathway databases

ReactomeiR-RNO-418359 Reduction of cytosolic Ca++ levels
R-RNO-5578775 Ion homeostasis
R-RNO-936837 Ion transport by P-type ATPases

Miscellaneous databases

PROiPR:Q64568

Gene expression databases

BgeeiENSRNOG00000061304 Expressed in 7 organ(s), highest expression level in brain
ExpressionAtlasiQ64568 baseline and differential

Family and domain databases

Gene3Di3.40.1110.10, 1 hit
3.40.50.1000, 1 hit
InterProiView protein in InterPro
IPR030325 ATP2B3
IPR022141 ATP_Ca_trans_C
IPR006068 ATPase_P-typ_cation-transptr_C
IPR004014 ATPase_P-typ_cation-transptr_N
IPR023299 ATPase_P-typ_cyto_dom_N
IPR018303 ATPase_P-typ_P_site
IPR023298 ATPase_P-typ_TM_dom_sf
IPR008250 ATPase_P-typ_transduc_dom_A_sf
IPR036412 HAD-like_sf
IPR023214 HAD_sf
IPR006408 P-type_ATPase_IIB
IPR001757 P_typ_ATPase
PANTHERiPTHR24093:SF284 PTHR24093:SF284, 1 hit
PfamiView protein in Pfam
PF12424 ATP_Ca_trans_C, 2 hits
PF00689 Cation_ATPase_C, 1 hit
PF00690 Cation_ATPase_N, 1 hit
SMARTiView protein in SMART
SM00831 Cation_ATPase_N, 1 hit
SUPFAMiSSF56784 SSF56784, 1 hit
SSF81653 SSF81653, 1 hit
SSF81660 SSF81660, 1 hit
SSF81665 SSF81665, 1 hit
TIGRFAMsiTIGR01517 ATPase-IIB_Ca, 1 hit
TIGR01494 ATPase_P-type, 3 hits
PROSITEiView protein in PROSITE
PS00154 ATPASE_E1_E2, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiAT2B3_RAT
AccessioniPrimary (citable) accession number: Q64568
Secondary accession number(s): Q01489, Q63444
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 1, 2001
Last sequence update: June 1, 2001
Last modified: November 7, 2018
This is version 157 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
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