UniProtKB - Q64559 (BACH_RAT)
Cytosolic acyl coenzyme A thioester hydrolase
Acot7
Functioni
Catalyzes the hydrolysis of acyl-CoAs into free fatty acids and coenzyme A (CoASH), regulating their respective intracellular levels (PubMed:7906114).
Preferentially hydrolyzes palmitoyl-CoA, but has a broad specificity acting on other fatty acyl-CoAs with chain-lengths of C8-C18 (PubMed:7906114).
May play an important physiological function in brain (PubMed:7906114).
1 PublicationCaution
Catalytic activityi
- EC:3.1.2.21 PublicationThis reaction proceeds in the forward1 Publication direction.
- This reaction proceeds in the forward1 Publication direction.
- This reaction proceeds in the forward1 Publication direction.
- This reaction proceeds in the forward1 Publication direction.
- This reaction proceeds in the forward1 Publication direction.
- This reaction proceeds in the forward1 Publication direction.
- This reaction proceeds in the forward1 Publication direction.
Kineticsi
- KM=5.9 µM for palmitoyl-CoA1 Publication
- Vmax=553 µmol/min/mg enzyme with palmitoyl-CoA as substrate1 Publication
: fatty acid metabolism Pathwayi
This protein is involved in the pathway fatty acid metabolism, which is part of Lipid metabolism.1 PublicationView all proteins of this organism that are known to be involved in the pathway fatty acid metabolism and in Lipid metabolism.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 67 | By similarity | 1 | |
Active sitei | 256 | By similarity | 1 |
GO - Molecular functioni
- acyl-CoA hydrolase activity Source: RGD
- carboxylic ester hydrolase activity Source: UniProtKB-KW
- fatty-acyl-CoA binding Source: RGD
- identical protein binding Source: RGD
- long-chain fatty acyl-CoA binding Source: RGD
- myristoyl-CoA hydrolase activity Source: UniProtKB-EC
- palmitoyl-CoA hydrolase activity Source: RGD
- protein homodimerization activity Source: RGD
GO - Biological processi
- acyl-CoA metabolic process Source: GO_Central
- coenzyme A biosynthetic process Source: RGD
- fatty acid catabolic process Source: RGD
- fatty acid metabolic process Source: GO_Central
- long-chain fatty-acyl-CoA catabolic process Source: RGD
- medium-chain fatty acid biosynthetic process Source: RGD
- medium-chain fatty-acyl-CoA catabolic process Source: RGD
- palmitic acid biosynthetic process Source: RGD
Keywordsi
Molecular function | Hydrolase, Serine esterase |
Biological process | Fatty acid metabolism, Lipid metabolism |
Enzyme and pathway databases
BRENDAi | 3.1.2.2, 5301 3.1.2.20, 5301 |
Reactomei | R-RNO-77289, Mitochondrial Fatty Acid Beta-Oxidation |
SABIO-RKi | Q64559 |
UniPathwayi | UPA00199 |
Chemistry databases
SwissLipidsi | SLP:000000586 SLP:000000614 [Q64559-1] |
Names & Taxonomyi
Protein namesi | Recommended name: Cytosolic acyl coenzyme A thioester hydrolase (EC:3.1.2.21 Publication)Alternative name(s): ACH1 ACT Acyl-CoA thioesterase 7 Brain acyl-CoA hydrolase Short name: BACH CTE-IIa CTE-IIb Short name: CTE-II LACH1 Long chain acyl-CoA thioester hydrolase MTE-II |
Gene namesi | Name:Acot7 Synonyms:Bach |
Organismi | Rattus norvegicus (Rat) |
Taxonomic identifieri | 10116 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Rattus |
Proteomesi |
|
Organism-specific databases
RGDi | 628856, Acot7 |
Subcellular locationi
Cytoplasm and Cytosol
- cytosol 1 Publication
Cytosol
- cytosol Source: RGD
Mitochondrion
- mitochondrial matrix Source: RGD
Nucleus
- nucleoplasm Source: GO_Central
Other locations
- cell body Source: RGD
- cytoplasm Source: RGD
- neuron projection Source: RGD
Keywords - Cellular componenti
CytoplasmPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000053808 | 1 – 381 | Cytosolic acyl coenzyme A thioester hydrolaseAdd BLAST | 381 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 169 | N6-acetyllysineBy similarity | 1 | |
Modified residuei | 199 | N6-acetyllysineBy similarity | 1 | |
Modified residuei | 284 | N6-acetyllysineBy similarity | 1 |
Post-translational modificationi
Keywords - PTMi
AcetylationProteomic databases
jPOSTi | Q64559 |
PaxDbi | Q64559 |
PRIDEi | Q64559 |
PTM databases
iPTMneti | Q64559 |
PhosphoSitePlusi | Q64559 |
Expressioni
Tissue specificityi
Gene expression databases
Bgeei | ENSRNOG00000010580, Expressed in testis and 21 other tissues |
ExpressionAtlasi | Q64559, baseline and differential |
Genevisiblei | Q64559, RN |
Interactioni
Subunit structurei
Homohexamer.
By similarityGO - Molecular functioni
- identical protein binding Source: RGD
- protein homodimerization activity Source: RGD
Protein-protein interaction databases
STRINGi | 10116.ENSRNOP00000014213 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 51 – 169 | HotDog ACOT-type 1PROSITE-ProRule annotationAdd BLAST | 119 | |
Domaini | 225 – 339 | HotDog ACOT-type 2PROSITE-ProRule annotationAdd BLAST | 115 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 343 – 381 | DisorderedSequence analysisAdd BLAST | 39 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 350 – 381 | Basic and acidic residuesSequence analysisAdd BLAST | 32 |
Domaini
Keywords - Domaini
RepeatPhylogenomic databases
eggNOGi | KOG2763, Eukaryota |
GeneTreei | ENSGT00940000155229 |
InParanoidi | Q64559 |
OrthoDBi | 1069806at2759 |
PhylomeDBi | Q64559 |
TreeFami | TF329579 |
Family and domain databases
InterProi | View protein in InterPro IPR040170, Cytosol_ACT IPR033120, HOTDOG_ACOT IPR029069, HotDog_dom_sf IPR006683, Thioestr_dom |
PANTHERi | PTHR11049, PTHR11049, 2 hits |
Pfami | View protein in Pfam PF03061, 4HBT, 2 hits |
SUPFAMi | SSF54637, SSF54637, 2 hits |
PROSITEi | View protein in PROSITE PS51770, HOTDOG_ACOT, 2 hits |
s (2+)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 2 described isoforms and 2 potential isoforms that are computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MKLLARTLYL WEVGRQVASW SLTSGQECLV LRETWWASMR AVRTRAVHHK
60 70 80 90 100
PGHCIAMGRI MRPDDANVAG NVHGGTILKM IEEAGVIIST RHCNSQNGER
110 120 130 140 150
CVAALARVER TDFLSPMCIG EVAHVSAEIT YTSKHSVEVQ VHVLSENILT
160 170 180 190 200
GTKKLTNKAT LWYVPLSLKN VDKVLEVPPI VYLRQEQEEE GRKRYEAQKL
210 220 230 240 250
ERMETKWRNG DIVQPILNPE PNTVSYSQSS LIHLVGPSDC TLHGFVHGGV
260 270 280 290 300
TMKLMDEVAG IVAARHCKTN IVTASVDAIN FHDKIRKGCV ITISGRMTFT
310 320 330 340 350
SNKSMEIEVL VDADPVVDNS QKRYRAASAF FTYVSLNQEG KPLPVPQLVP
360 370 380
ETEDEKKRFE EGKGRYLQMK AKRQGHTEPQ P
Computationally mapped potential isoform sequencesi
There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketF8WG67 | F8WG67_RAT | Cytosolic acyl coenzyme A thioester... | Acot7 | 349 | Annotation score: | ||
A0A8I6ACH7 | A0A8I6ACH7_RAT | Cytosolic acyl coenzyme A thioester... | Acot7 | 381 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 86 | V → A in AAC53202 (PubMed:8631842).Curated | 1 | |
Sequence conflicti | 89 | S → R in AAC53202 (PubMed:8631842).Curated | 1 | |
Sequence conflicti | 144 | L → M in AAC53202 (PubMed:8631842).Curated | 1 | |
Sequence conflicti | 171 | V → A in BAA19627 (PubMed:9125130).Curated | 1 | |
Sequence conflicti | 216 | I → V in AAC53202 (PubMed:8631842).Curated | 1 | |
Sequence conflicti | 279 | I → V in BAA19627 (PubMed:9125130).Curated | 1 | |
Sequence conflicti | 343 | L → M in AAC53202 (PubMed:8631842).Curated | 1 | |
Sequence conflicti | 372 | K → N in AAC53202 (PubMed:8631842).Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_016956 | 1 – 58 | MKLLA…CIAMG → MSGPTTDTPAAIQIC in isoform 1. 4 PublicationsAdd BLAST | 58 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U49694 mRNA Translation: AAC53202.1 D88890 mRNA Translation: BAA19626.1 D88891 mRNA Translation: BAA19627.2 Y09332 mRNA Translation: CAA70512.1 BC087716 mRNA Translation: AAH87716.1 |
PIRi | JC5415 JC5416 |
RefSeqi | NP_001139533.1, NM_001146061.1 [Q64559-2] NP_037346.2, NM_013214.4 |
Genome annotation databases
GeneIDi | 26759 |
KEGGi | rno:26759 |
UCSCi | RGD:628856, rat [Q64559-2] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U49694 mRNA Translation: AAC53202.1 D88890 mRNA Translation: BAA19626.1 D88891 mRNA Translation: BAA19627.2 Y09332 mRNA Translation: CAA70512.1 BC087716 mRNA Translation: AAH87716.1 |
PIRi | JC5415 JC5416 |
RefSeqi | NP_001139533.1, NM_001146061.1 [Q64559-2] NP_037346.2, NM_013214.4 |
3D structure databases
AlphaFoldDBi | Q64559 |
SMRi | Q64559 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 10116.ENSRNOP00000014213 |
Chemistry databases
SwissLipidsi | SLP:000000586 SLP:000000614 [Q64559-1] |
PTM databases
iPTMneti | Q64559 |
PhosphoSitePlusi | Q64559 |
Proteomic databases
jPOSTi | Q64559 |
PaxDbi | Q64559 |
PRIDEi | Q64559 |
Genome annotation databases
GeneIDi | 26759 |
KEGGi | rno:26759 |
UCSCi | RGD:628856, rat [Q64559-2] |
Organism-specific databases
CTDi | 11332 |
RGDi | 628856, Acot7 |
Phylogenomic databases
eggNOGi | KOG2763, Eukaryota |
GeneTreei | ENSGT00940000155229 |
InParanoidi | Q64559 |
OrthoDBi | 1069806at2759 |
PhylomeDBi | Q64559 |
TreeFami | TF329579 |
Enzyme and pathway databases
UniPathwayi | UPA00199 |
BRENDAi | 3.1.2.2, 5301 3.1.2.20, 5301 |
Reactomei | R-RNO-77289, Mitochondrial Fatty Acid Beta-Oxidation |
SABIO-RKi | Q64559 |
Miscellaneous databases
PROi | PR:Q64559 |
Gene expression databases
Bgeei | ENSRNOG00000010580, Expressed in testis and 21 other tissues |
ExpressionAtlasi | Q64559, baseline and differential |
Genevisiblei | Q64559, RN |
Family and domain databases
InterProi | View protein in InterPro IPR040170, Cytosol_ACT IPR033120, HOTDOG_ACOT IPR029069, HotDog_dom_sf IPR006683, Thioestr_dom |
PANTHERi | PTHR11049, PTHR11049, 2 hits |
Pfami | View protein in Pfam PF03061, 4HBT, 2 hits |
SUPFAMi | SSF54637, SSF54637, 2 hits |
PROSITEi | View protein in PROSITE PS51770, HOTDOG_ACOT, 2 hits |
MobiDBi | Search... |
Entry informationi
Entry namei | BACH_RAT | |
Accessioni | Q64559Primary (citable) accession number: Q64559 Secondary accession number(s): O08652, O09041 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 1, 1997 |
Last sequence update: | January 10, 2006 | |
Last modified: | May 25, 2022 | |
This is version 151 of the entry and version 4 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Direct protein sequencing, Reference proteomeDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways