UniProtKB - Q64550 (UD11_RAT)
Protein
UDP-glucuronosyltransferase 1A1
Gene
Ugt1a1
Organism
Rattus norvegicus (Rat)
Status
Functioni
UDP-glucuronosyltransferase (UGT) that catalyzes phase II biotransformation reactions in which lipophilic substrates are conjugated with glucuronic acid to increase the metabolite's water solubility, thereby facilitating excretion into either the urine or bile (PubMed:8554318). Essential for the elimination and detoxification of drugs, xenobiotics and endogenous compounds (PubMed:8554318). Catalyzes the glucuronidation of endogenous estrogen hormones such as estradiol, estrone and estriol (By similarity). Involved in the glucuronidation of bilirubin, a degradation product occurring in the normal catabolic pathway that breaks down heme in vertebrates (By similarity). Also catalyzes the glucuronidation the isoflavones genistein, daidzein, glycitein, formononetin, biochanin A and prunetin, which are phytoestrogens with anticancer and cardiovascular properties (By similarity). Involved in the glucuronidation of the AGTR1 angiotensin receptor antagonist losartan, a drug which can inhibit the effect of angiotensin II (By similarity). Involved in the biotransformation of 7-ethyl-10-hydroxycamptothecin (SN-38), the pharmacologically active metabolite of the anticancer drug irinotecan (By similarity).By similarity1 Publication
Catalytic activityi
- EC:2.4.1.17By similarityThis reaction proceeds in the forwardBy similarity direction.
- This reaction proceeds in the forwardBy similarity direction.
- 2-hydroxyestrone + UDP-α-D-glucuronate = 2-hydroxyestrone 3-O-(β-D-glucuronate) + H+ + UDPBy similarityThis reaction proceeds in the forwardBy similarity direction.
- 2-hydroxy-17β-estradiol + UDP-α-D-glucuronate = 2-hydroxy-17β-estradiol 3-O-(β-D-glucuronate) + H+ + UDPBy similarityThis reaction proceeds in the forwardBy similarity direction.
- 2-methoxy-17β-estradiol + UDP-α-D-glucuronate = 2-methoxy-17β-estradiol 3-O-(β-D-glucuronate) + H+ + UDPBy similarityThis reaction proceeds in the forwardBy similarity direction.
- This reaction proceeds in the forwardBy similarity direction.
- 16β,17β-estriol + UDP-α-D-glucuronate = 16β,17β-estriol 16-O-(β-D-glucuronate) + H+ + UDPBy similarityThis reaction proceeds in the forwardBy similarity direction.
- This reaction proceeds in the forwardBy similarity direction.
- This reaction proceeds in the forwardBy similarity direction.
- This reaction proceeds in the forwardBy similarity direction.
GO - Molecular functioni
- enzyme binding Source: RGD
- enzyme inhibitor activity Source: RGD
- glucuronosyltransferase activity Source: RGD
- protein heterodimerization activity Source: RGD
- protein homodimerization activity Source: RGD
- retinoic acid binding Source: RGD
- steroid binding Source: RGD
GO - Biological processi
- acute-phase response Source: RGD
- animal organ regeneration Source: RGD
- bilirubin conjugation Source: RGD
- biphenyl catabolic process Source: RGD
- cellular glucuronidation Source: RGD
- cellular response to estradiol stimulus Source: RGD
- cellular response to ethanol Source: RGD
- cellular response to glucocorticoid stimulus Source: RGD
- cellular response to hormone stimulus Source: RGD
- cellular response to xenobiotic stimulus Source: RGD
- flavone metabolic process Source: RGD
- flavonoid glucuronidation Source: RGD
- liver development Source: RGD
- negative regulation of cellular glucuronidation Source: RGD
- response to drug Source: RGD
- response to ethanol Source: RGD
- response to glucocorticoid Source: RGD
- response to lipopolysaccharide Source: RGD
- response to nutrient Source: RGD
- response to organic cyclic compound Source: RGD
- response to organic substance Source: RGD
- response to starvation Source: RGD
- response to steroid hormone Source: RGD
- xenobiotic glucuronidation Source: RGD
Keywordsi
Molecular function | Glycosyltransferase, Transferase |
Enzyme and pathway databases
BRENDAi | 2.4.1.17, 5301 |
Reactomei | R-RNO-156588, Glucuronidation |
Protein family/group databases
CAZyi | GT1, Glycosyltransferase Family 1 |
Names & Taxonomyi
Protein namesi | Recommended name: UDP-glucuronosyltransferase 1A1Curated (EC:2.4.1.17By similarity)Short name: UGT1A1 Alternative name(s): B1 UDP-glucuronosyltransferase 1-1 Short name: UDPGT 1-1 Short name: UGT1*1 Short name: UGT1-01 Short name: UGT1.1 |
Gene namesi | |
Organismi | Rattus norvegicus (Rat) |
Taxonomic identifieri | 10116 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Rattus |
Proteomesi |
|
Organism-specific databases
RGDi | 3935, Ugt1a1 |
Subcellular locationi
Endoplasmic reticulum
- Endoplasmic reticulum membrane By similarity; Single-pass membrane protein Sequence analysis
Endoplasmic reticulum
- endoplasmic reticulum Source: HGNC-UCL
- endoplasmic reticulum chaperone complex Source: RGD
- endoplasmic reticulum membrane Source: UniProtKB-SubCell
Plasma Membrane
- integral component of plasma membrane Source: RGD
Other locations
- cytochrome complex Source: RGD
- intracellular membrane-bounded organelle Source: RGD
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transmembranei | 493 – 509 | HelicalSequence analysisAdd BLAST | 17 |
Keywords - Cellular componenti
Endoplasmic reticulum, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 29 | 1 PublicationAdd BLAST | 29 | |
ChainiPRO_0000036011 | 30 – 535 | UDP-glucuronosyltransferase 1A1Add BLAST | 506 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Glycosylationi | 89 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 297 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 435 | N-linked (GlcNAc...) asparagineSequence analysis | 1 |
Keywords - PTMi
GlycoproteinProteomic databases
PaxDbi | Q64550 |
PRIDEi | Q64550 |
PTM databases
GlyGeni | Q64550, 3 sites |
SwissPalmi | Q64550 |
Expressioni
Inductioni
Induced in 3-methylcholanthrene-treated rats.1 Publication
Gene expression databases
Bgeei | ENSRNOG00000018740, Expressed in duodenum and 22 other tissues |
ExpressionAtlasi | Q64550, baseline and differential |
Genevisiblei | Q64550, RN |
Interactioni
Subunit structurei
Homodimers. Homooligomer.
Interacts with UGT1A3, UGT1A4, UGT1A6, UGT1A7, UGT1A8, UGT1A9 and UGT1A10 to form heterodimers.
By similarityGO - Molecular functioni
- enzyme binding Source: RGD
- protein heterodimerization activity Source: RGD
- protein homodimerization activity Source: RGD
Protein-protein interaction databases
IntActi | Q64550, 1 interactor |
STRINGi | 10116.ENSRNOP00000025045 |
Family & Domainsi
Sequence similaritiesi
Belongs to the UDP-glycosyltransferase family.Curated
Keywords - Domaini
Signal, Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG1192, Eukaryota |
GeneTreei | ENSGT00940000159677 |
HOGENOMi | CLU_012949_3_1_1 |
InParanoidi | Q64550 |
TreeFami | TF315472 |
Family and domain databases
InterProi | View protein in InterPro IPR002213, UDP_glucos_trans IPR035595, UDP_glycos_trans_CS |
Pfami | View protein in Pfam PF00201, UDPGT, 1 hit |
PROSITEi | View protein in PROSITE PS00375, UDPGT, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry describes 1 produced by isoform ialternative splicing. AlignAdd to basketNote: UGT1A1 is one of the isoforms produced at the UGT1A complex locus. The UGT1A complex locus produces different isoforms based on alternative use of promoters, first exons and terminal exons.By similarity
Isoform 1 (identifier: Q64550-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MSVVCRSSCS LLLLPCLLLC VLGPSASHAG KLLVIPIDGS HWLSMLGVIQ
60 70 80 90 100
QLQQKGHEVV VIAPEASIHI KEGSFYTMRK YPVPFQNENV TAAFVELGRS
110 120 130 140 150
VFDQDPFLLR VVKTYNKVKR DSSMLLSGCS HLLHNAEFMA SLEQSHFDAL
160 170 180 190 200
LTDPFLPCGS IVAQYLSLPA VYFLNALPCS LDLEATQCPA PLSYVPKSLS
210 220 230 240 250
SNTDRMNFLQ RVKNMIIALT ENFLCRVVYS PYGSLATEIL QKEVTVKDLL
260 270 280 290 300
SPASIWLMRN DFVKDYPRPI MPNMVFIGGI NCLQKKALSQ EFEAYVNASG
310 320 330 340 350
EHGIVVFSLG SMVSEIPEKK AMEIAEALGR IPQTVLWRYT GTRPSNLAKN
360 370 380 390 400
TILVKWLPQN DLLGHPKARA FITHSGSHGI YEGICNGVPM VMMPLFGDQM
410 420 430 440 450
DNAKRMETRG AGVTLNVLEM TADDLENALK TVINNKSYKE NIMRLSSLHK
460 470 480 490 500
DRPIEPLDLA VFWVEYVMRH KGAPHLRPAA HDLTWYQYHS LDVIGFLLAI
510 520 530
VLTVVFIVYK SCAYGCRKCF GGKGRVKKSH KSKTH
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 253 | A → D in BAA07260 (PubMed:7608130).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U20551 Genomic DNA Translation: AAC52219.1 D38065 Genomic DNA Translation: BAA07260.1 |
PIRi | I57961 |
RefSeqi | NP_036815.1, NM_012683.2 [Q64550-1] |
Genome annotation databases
Ensembli | ENSRNOT00000025045; ENSRNOP00000025045; ENSRNOG00000018740 [Q64550-1] |
GeneIDi | 24861 |
KEGGi | rno:24861 |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U20551 Genomic DNA Translation: AAC52219.1 D38065 Genomic DNA Translation: BAA07260.1 |
PIRi | I57961 |
RefSeqi | NP_036815.1, NM_012683.2 [Q64550-1] |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
IntActi | Q64550, 1 interactor |
STRINGi | 10116.ENSRNOP00000025045 |
Protein family/group databases
CAZyi | GT1, Glycosyltransferase Family 1 |
PTM databases
GlyGeni | Q64550, 3 sites |
SwissPalmi | Q64550 |
Proteomic databases
PaxDbi | Q64550 |
PRIDEi | Q64550 |
Genome annotation databases
Ensembli | ENSRNOT00000025045; ENSRNOP00000025045; ENSRNOG00000018740 [Q64550-1] |
GeneIDi | 24861 |
KEGGi | rno:24861 |
Organism-specific databases
CTDi | 54658 |
RGDi | 3935, Ugt1a1 |
Phylogenomic databases
eggNOGi | KOG1192, Eukaryota |
GeneTreei | ENSGT00940000159677 |
HOGENOMi | CLU_012949_3_1_1 |
InParanoidi | Q64550 |
TreeFami | TF315472 |
Enzyme and pathway databases
BRENDAi | 2.4.1.17, 5301 |
Reactomei | R-RNO-156588, Glucuronidation |
Miscellaneous databases
PROi | PR:Q64550 |
Gene expression databases
Bgeei | ENSRNOG00000018740, Expressed in duodenum and 22 other tissues |
ExpressionAtlasi | Q64550, baseline and differential |
Genevisiblei | Q64550, RN |
Family and domain databases
InterProi | View protein in InterPro IPR002213, UDP_glucos_trans IPR035595, UDP_glycos_trans_CS |
Pfami | View protein in Pfam PF00201, UDPGT, 1 hit |
PROSITEi | View protein in PROSITE PS00375, UDPGT, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | UD11_RAT | |
Accessioni | Q64550Primary (citable) accession number: Q64550 Secondary accession number(s): Q64635 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 1, 1997 |
Last sequence update: | November 1, 1997 | |
Last modified: | December 2, 2020 | |
This is version 140 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Direct protein sequencing, Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families