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Protein

Plasma membrane calcium-transporting ATPase 4

Gene

Atp2b4

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Calcium/calmodulin-regulated and magnesium-dependent enzyme that catalyzes the hydrolysis of ATP coupled with the transport of calcium out of the cell (By similarity). By regulating sperm cell calcium homeostasis, may play a role in sperm motility (By similarity).By similarity

Catalytic activityi

ATP + H2O + Ca2+(Side 1) = ADP + phosphate + Ca2+(Side 2).By similarity

Activity regulationi

Activated by calcium/calmodulin.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei4654-aspartylphosphate intermediateBy similarity1
Metal bindingi785MagnesiumBy similarity1
Metal bindingi789MagnesiumBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionCalmodulin-binding, Hydrolase
Biological processCalcium transport, Ion transport, Transport
LigandATP-binding, Calcium, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-RNO-418359 Reduction of cytosolic Ca++ levels
R-RNO-5578775 Ion homeostasis
R-RNO-936837 Ion transport by P-type ATPases

Names & Taxonomyi

Protein namesi
Recommended name:
Plasma membrane calcium-transporting ATPase 4Curated (EC:3.6.3.8By similarity)
Short name:
PMCA42 Publications
Alternative name(s):
Plasma membrane calcium ATPase isoform 41 Publication
Plasma membrane calcium pump isoform 41 Publication
Gene namesi
Name:Atp2b4Imported
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 13

Organism-specific databases

RGDi621305 Atp2b4

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 92CytoplasmicSequence analysisAdd BLAST92
Transmembranei93 – 113HelicalSequence analysisAdd BLAST21
Topological domaini114 – 150ExtracellularSequence analysisAdd BLAST37
Transmembranei151 – 171HelicalSequence analysisAdd BLAST21
Topological domaini172 – 356CytoplasmicSequence analysisAdd BLAST185
Transmembranei357 – 376HelicalSequence analysisAdd BLAST20
Topological domaini377 – 409ExtracellularSequence analysisAdd BLAST33
Transmembranei410 – 427HelicalSequence analysisAdd BLAST18
Topological domaini428 – 840CytoplasmicSequence analysisAdd BLAST413
Transmembranei841 – 860HelicalSequence analysisAdd BLAST20
Topological domaini861 – 870ExtracellularSequence analysis10
Transmembranei871 – 891HelicalSequence analysisAdd BLAST21
Topological domaini892 – 911CytoplasmicSequence analysisAdd BLAST20
Transmembranei912 – 934HelicalSequence analysisAdd BLAST23
Topological domaini935 – 952ExtracellularSequence analysisAdd BLAST18
Transmembranei953 – 974HelicalSequence analysisAdd BLAST22
Topological domaini975 – 993CytoplasmicSequence analysisAdd BLAST19
Transmembranei994 – 1015HelicalSequence analysisAdd BLAST22
Topological domaini1016 – 1025ExtracellularSequence analysis10
Transmembranei1026 – 1047HelicalSequence analysisAdd BLAST22
Topological domaini1048 – 1203CytoplasmicSequence analysisAdd BLAST156

Keywords - Cellular componenti

Cell membrane, Cell projection, Cilium, Flagellum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000462211 – 1203Plasma membrane calcium-transporting ATPase 4Add BLAST1203

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei13PhosphoserineBy similarity1
Modified residuei328PhosphoserineCombined sources1
Modified residuei334PhosphoserineCombined sources1
Modified residuei1064PhosphoserineCombined sources1
Modified residuei1070PhosphoserineCombined sources1
Modified residuei1102Phosphothreonine; by PKCBy similarity1
Modified residuei1144PhosphoserineBy similarity1
Isoform ZA (identifier: Q64542-3)
Modified residuei1103PhosphoserineCombined sources1
Modified residuei1114PhosphoserineCombined sources1
Isoform XA (identifier: Q64542-2)
Modified residuei1115PhosphoserineCombined sources1
Modified residuei1126PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ64542
PRIDEiQ64542

PTM databases

iPTMnetiQ64542
PhosphoSitePlusiQ64542

Expressioni

Tissue specificityi

Ubiquitously expressed. Not detected in liver. The highest levels are found in uterus and stomach. Isoform XA is found in uterus, brain, stomach, small intestine, colon and pancreas. Isoform XB is found in uterus, skeletal muscle, lung, kidney, spleen, stomach, small intestine and pancreas. Isoform ZA is found in testis and isoform ZB is found in testis and heart.2 Publications

Gene expression databases

BgeeiENSRNOG00000003031 Expressed in 10 organ(s), highest expression level in brain
GenevisibleiQ64542 RN

Interactioni

Subunit structurei

Interacts with PDZD11. Interacts with SLC35G1 and STIM1. Interacts with calmodulin.By similarity

GO - Molecular functioni

Protein-protein interaction databases

BioGridi248232, 1 interactor
IntActiQ64542, 1 interactor
MINTiQ64542
STRINGi10116.ENSRNOP00000004078

Structurei

3D structure databases

ProteinModelPortaliQ64542
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1086 – 1103Calmodulin-binding subdomain ABy similarityAdd BLAST18
Regioni1104 – 1113Calmodulin-binding subdomain BBy similarity10

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi297 – 303Poly-Lys7

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0204 Eukaryota
ENOG410XNNC LUCA
GeneTreeiENSGT00510000046331
HOGENOMiHOG000265623
HOVERGENiHBG061286
InParanoidiQ64542
KOiK05850
OMAiTQWIFSI
OrthoDBiEOG091G057D
PhylomeDBiQ64542

Family and domain databases

Gene3Di3.40.1110.10, 1 hit
3.40.50.1000, 1 hit
InterProiView protein in InterPro
IPR034304 ATP2B4
IPR022141 ATP_Ca_trans_C
IPR006068 ATPase_P-typ_cation-transptr_C
IPR004014 ATPase_P-typ_cation-transptr_N
IPR023299 ATPase_P-typ_cyto_dom_N
IPR018303 ATPase_P-typ_P_site
IPR023298 ATPase_P-typ_TM_dom_sf
IPR008250 ATPase_P-typ_transduc_dom_A_sf
IPR036412 HAD-like_sf
IPR023214 HAD_sf
IPR006408 P-type_ATPase_IIB
IPR001757 P_typ_ATPase
PANTHERiPTHR24093:SF276 PTHR24093:SF276, 1 hit
PfamiView protein in Pfam
PF12424 ATP_Ca_trans_C, 1 hit
PF00689 Cation_ATPase_C, 1 hit
PF00690 Cation_ATPase_N, 1 hit
SMARTiView protein in SMART
SM00831 Cation_ATPase_N, 1 hit
SUPFAMiSSF56784 SSF56784, 1 hit
SSF81653 SSF81653, 1 hit
SSF81660 SSF81660, 1 hit
SSF81665 SSF81665, 1 hit
TIGRFAMsiTIGR01517 ATPase-IIB_Ca, 1 hit
TIGR01494 ATPase_P-type, 3 hits
PROSITEiView protein in PROSITE
PS00154 ATPASE_E1_E2, 1 hit

Sequences (4)i

Sequence statusi: Complete.

This entry describes 4 isoformsi produced by alternative splicing. AlignAdd to basket
Note: There is a combination of two alternatively spliced domains at N-terminal site A (X and Z) and at C-terminal site C (A and B).
Isoform XB (identifier: Q64542-1) [UniParc]FASTAAdd to basket
Also known as: AIICI

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MTNPSGHNLP ANSVAESYEG EFGCTLMDLR KLMELRAADA VTQISAHYGS
60 70 80 90 100
VQEICARLKT SPVEGLSGNP ADLEKRRLVF GKNMIPPKKP KTFLELVWEA
110 120 130 140 150
LQDVTLIILE IAAIISLVLS FYRPPGGENE ICGHIVSNPE EDEEGETGWI
160 170 180 190 200
EGAAILASVI IVVFVTAFND WSKEKQFRGL QSRIELEQKF SIIRNGQLIQ
210 220 230 240 250
LPVAEIVVGD IAQVKYGDLL PADGILIQGN DLKIDESSLT GESDHVKKTL
260 270 280 290 300
DKDPMLLSGT HVMEGSGRMV VTAVGINSQT GIIFTLLGAN EEEDDEKKKK
310 320 330 340 350
GKKQGVSENR NKAKTQDGVA LEIQPLNSQE GLDSEEKEKK ASKGPKKEKS
360 370 380 390 400
VLQGKLTRLA VQIGKAGLIM SILTVLILIL YFVVDNFVIQ RRAWLPECTP
410 420 430 440 450
VYIQYFVKFF IIGVTVLVVA VPEGLPLAVT ISLAYSVKKM MKDNNLVRHL
460 470 480 490 500
DACETMGNAT AICSDKTGTL TMNRMTVVQA YIGGTHYRQI PKPDDLPPNV
510 520 530 540 550
LDLIVNSICI NSAYTSKILP PEKEGGLPRQ VGNKTECGLL GFVTDLKQDY
560 570 580 590 600
QAVRSEMPEE KLFKVYTFNS VRKSMSTVIR KPEGGFRVFS KGASEIMLRK
610 620 630 640 650
CDRILNKEGG IVPFKTKDRD NMVRNVIEPM ASEGLRTIGI AYRDFDGEEP
660 670 680 690 700
SWENENEIFT GLVCIAVVGI EDPVRPEVPD AINKCKRAGI TVRMVTGDNV
710 720 730 740 750
NTARAIATKC GILTPGDDFL CLEGKEFNRL IRNEKGEVEQ EKLDKVWPRL
760 770 780 790 800
RVLARSSPTD KHTLVKGIID SNIGEQRQVV AVTGDGTNDG PALKKADVGF
810 820 830 840 850
AMGIAGTDVA KEASDIILTD DNFTSIVKAV MWGRNVYDSI SKFLQFQLTV
860 870 880 890 900
NVVAVIVAFS GACITQDSPL KAVQMLWVNL IMDTFASLAL ATEPPTDSLL
910 920 930 940 950
RRRPYGRNKP LISRTMMKNI LGHAVYQLGI VFLLVFAGDK LFDIDSGRKA
960 970 980 990 1000
PLNSPPSQHY TIVFNTFVLM QLFNEINSRK IHGEKNVFAG VYRNIIFCSV
1010 1020 1030 1040 1050
VLGTFFCQIL IVEVGGKPFS CTNLTMEQWM WCLFIGIGEL LWGQVISAIP
1060 1070 1080 1090 1100
TKSLKFLKEA GHGSDKEEIS KDAEGLEEID HAEMELRRGQ ILWVRGLNRI
1110 1120 1130 1140 1150
QTQIRVVKVF HSFRDVIHKS KNQVSIHSFM TQPEYAADDE MSQSFLNQEE
1160 1170 1180 1190 1200
SPSLASKSRI TKRLSDAETV SQNNTNNNAV DCHQVQIVAS HPNSPLQSQE

TPV
Length:1,203
Mass (Da):133,094
Last modified:November 1, 1996 - v1
Checksum:iB7A6C8D4556F6398
GO
Isoform XA (identifier: Q64542-2) [UniParc]FASTAAdd to basket
Also known as: AIICII

The sequence of this isoform differs from the canonical sequence as follows:
     1105-1203: RVVKVFHSFR...SPLQSQETPV → EVINKFQTGA...VGNQSGQSIS

Show »
Length:1,169
Mass (Da):128,791
Checksum:iC799FA6AC708EBE4
GO
Isoform ZA (identifier: Q64542-3) [UniParc]FASTAAdd to basket
Also known as: AICII

The sequence of this isoform differs from the canonical sequence as follows:
     301-312: Missing.
     1105-1203: RVVKVFHSFR...SPLQSQETPV → EVINKFQTGA...VGNQSGQSIS

Show »
Length:1,157
Mass (Da):127,464
Checksum:iEDB410076D59B247
GO
Isoform ZB (identifier: Q64542-4) [UniParc]FASTAAdd to basket
Also known as: AICI

The sequence of this isoform differs from the canonical sequence as follows:
     301-312: Missing.

Show »
Length:1,191
Mass (Da):131,768
Checksum:iD93D8B4839575FDB
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti431I → V in CAA53990 (PubMed:7945253).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_000406301 – 312Missing in isoform ZA and isoform ZB. 1 PublicationAdd BLAST12
Alternative sequenceiVSP_0004071105 – 1203RVVKV…QETPV → EVINKFQTGASFKGVLRRQN LSQQLDVKLVPSSYSEAVAS VRTSPSTSSAVTPPPVGNQS GQSIS in isoform XA and isoform ZA. 1 PublicationAdd BLAST99

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U15408 mRNA Translation: AAA81005.1
U15408 mRNA Translation: AAA81006.1
U15408 mRNA Translation: AAA81007.1
U15408 mRNA Translation: AAA81008.1
X76452 mRNA Translation: CAA53990.1
L05569, L05566, L05567 Genomic DNA Translation: AAA50820.1
PIRiG44525
S50027
S54356
S54357
RefSeqiNP_001005871.1, NM_001005871.1 [Q64542-2]
XP_008767668.1, XM_008769446.2 [Q64542-1]
XP_008767669.1, XM_008769447.2 [Q64542-1]
XP_008767670.1, XM_008769448.2 [Q64542-4]
XP_008767671.1, XM_008769449.2 [Q64542-2]
XP_008767672.1, XM_008769450.2 [Q64542-3]
UniGeneiRn.9986

Genome annotation databases

EnsembliENSRNOT00000004078; ENSRNOP00000004078; ENSRNOG00000003031 [Q64542-1]
ENSRNOT00000046273; ENSRNOP00000039880; ENSRNOG00000003031 [Q64542-2]
GeneIDi29600
KEGGirno:29600
UCSCiRGD:621305 rat [Q64542-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U15408 mRNA Translation: AAA81005.1
U15408 mRNA Translation: AAA81006.1
U15408 mRNA Translation: AAA81007.1
U15408 mRNA Translation: AAA81008.1
X76452 mRNA Translation: CAA53990.1
L05569, L05566, L05567 Genomic DNA Translation: AAA50820.1
PIRiG44525
S50027
S54356
S54357
RefSeqiNP_001005871.1, NM_001005871.1 [Q64542-2]
XP_008767668.1, XM_008769446.2 [Q64542-1]
XP_008767669.1, XM_008769447.2 [Q64542-1]
XP_008767670.1, XM_008769448.2 [Q64542-4]
XP_008767671.1, XM_008769449.2 [Q64542-2]
XP_008767672.1, XM_008769450.2 [Q64542-3]
UniGeneiRn.9986

3D structure databases

ProteinModelPortaliQ64542
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi248232, 1 interactor
IntActiQ64542, 1 interactor
MINTiQ64542
STRINGi10116.ENSRNOP00000004078

PTM databases

iPTMnetiQ64542
PhosphoSitePlusiQ64542

Proteomic databases

PaxDbiQ64542
PRIDEiQ64542

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000004078; ENSRNOP00000004078; ENSRNOG00000003031 [Q64542-1]
ENSRNOT00000046273; ENSRNOP00000039880; ENSRNOG00000003031 [Q64542-2]
GeneIDi29600
KEGGirno:29600
UCSCiRGD:621305 rat [Q64542-1]

Organism-specific databases

CTDi493
RGDi621305 Atp2b4

Phylogenomic databases

eggNOGiKOG0204 Eukaryota
ENOG410XNNC LUCA
GeneTreeiENSGT00510000046331
HOGENOMiHOG000265623
HOVERGENiHBG061286
InParanoidiQ64542
KOiK05850
OMAiTQWIFSI
OrthoDBiEOG091G057D
PhylomeDBiQ64542

Enzyme and pathway databases

ReactomeiR-RNO-418359 Reduction of cytosolic Ca++ levels
R-RNO-5578775 Ion homeostasis
R-RNO-936837 Ion transport by P-type ATPases

Miscellaneous databases

PROiPR:Q64542

Gene expression databases

BgeeiENSRNOG00000003031 Expressed in 10 organ(s), highest expression level in brain
GenevisibleiQ64542 RN

Family and domain databases

Gene3Di3.40.1110.10, 1 hit
3.40.50.1000, 1 hit
InterProiView protein in InterPro
IPR034304 ATP2B4
IPR022141 ATP_Ca_trans_C
IPR006068 ATPase_P-typ_cation-transptr_C
IPR004014 ATPase_P-typ_cation-transptr_N
IPR023299 ATPase_P-typ_cyto_dom_N
IPR018303 ATPase_P-typ_P_site
IPR023298 ATPase_P-typ_TM_dom_sf
IPR008250 ATPase_P-typ_transduc_dom_A_sf
IPR036412 HAD-like_sf
IPR023214 HAD_sf
IPR006408 P-type_ATPase_IIB
IPR001757 P_typ_ATPase
PANTHERiPTHR24093:SF276 PTHR24093:SF276, 1 hit
PfamiView protein in Pfam
PF12424 ATP_Ca_trans_C, 1 hit
PF00689 Cation_ATPase_C, 1 hit
PF00690 Cation_ATPase_N, 1 hit
SMARTiView protein in SMART
SM00831 Cation_ATPase_N, 1 hit
SUPFAMiSSF56784 SSF56784, 1 hit
SSF81653 SSF81653, 1 hit
SSF81660 SSF81660, 1 hit
SSF81665 SSF81665, 1 hit
TIGRFAMsiTIGR01517 ATPase-IIB_Ca, 1 hit
TIGR01494 ATPase_P-type, 3 hits
PROSITEiView protein in PROSITE
PS00154 ATPASE_E1_E2, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiAT2B4_RAT
AccessioniPrimary (citable) accession number: Q64542
Secondary accession number(s): Q63127
, Q63445, Q64543, Q64544, Q64545
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 1, 2001
Last sequence update: November 1, 1996
Last modified: November 7, 2018
This is version 168 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
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Main funding by: National Institutes of Health

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