The new UniProt website is here!
Take me to UniProt BETA
UniProtKB - Q64541 (AT1A4_RAT)
Protein
Sodium/potassium-transporting ATPase subunit alpha-4
Gene
Atp1a4
Organism
Rattus norvegicus (Rat)
Status
Functioni
This is the catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of sodium and potassium ions across the plasma membrane. This action creates the electrochemical gradient of sodium and potassium ions, providing the energy for active transport of various nutrients. Plays a role in sperm motility.
1 PublicationCatalytic activityi
Activity regulationi
Specifically inhibited by an endogenous cardiac glycoside, ouabain.By similarity
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 382 | 4-aspartylphosphate intermediateBy similarity | 1 | |
Metal bindingi | 722 | MagnesiumBy similarity | 1 | |
Metal bindingi | 726 | MagnesiumBy similarity | 1 |
GO - Molecular functioni
- ATPase-coupled cation transmembrane transporter activity Source: RGD
- ATP binding Source: UniProtKB-KW
- ATP hydrolysis activity Source: InterPro
- metal ion binding Source: UniProtKB-KW
- P-type sodium:potassium-exchanging transporter activity Source: MGI
GO - Biological processi
- cellular potassium ion homeostasis Source: GO_Central
- cellular sodium ion homeostasis Source: GO_Central
- fertilization Source: RGD
- flagellated sperm motility Source: RGD
- potassium ion import across plasma membrane Source: GO_Central
- potassium ion transmembrane transport Source: RGD
- potassium ion transport Source: RGD
- proton transmembrane transport Source: GO_Central
- regulation of cellular pH Source: RGD
- regulation of membrane potential Source: RGD
- sodium ion export across plasma membrane Source: GO_Central
- sodium ion transmembrane transport Source: RGD
- sodium ion transport Source: RGD
- spermatogenesis Source: RGD
Keywordsi
Molecular function | Translocase |
Biological process | Ion transport, Potassium transport, Sodium transport, Sodium/potassium transport, Transport |
Ligand | ATP-binding, Magnesium, Metal-binding, Nucleotide-binding, Potassium, Sodium |
Enzyme and pathway databases
Reactomei | R-RNO-5578775, Ion homeostasis R-RNO-936837, Ion transport by P-type ATPases |
SABIO-RKi | Q64541 |
Names & Taxonomyi
Protein namesi | Recommended name: Sodium/potassium-transporting ATPase subunit alpha-4 (EC:7.2.2.131 Publication)Short name: Na(+)/K(+) ATPase alpha-4 subunit Alternative name(s): Sodium pump subunit alpha-4 |
Gene namesi | Name:Atp1a4 Synonyms:Atp1al2 |
Organismi | Rattus norvegicus (Rat) |
Taxonomic identifieri | 10116 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Rattus |
Proteomesi |
|
Organism-specific databases
RGDi | 61952, Atp1a4 |
Subcellular locationi
Plasma membrane
Plasma Membrane
- sodium:potassium-exchanging ATPase complex Source: RGD
Other locations
- photoreceptor cell cilium Source: RGD
- rod photoreceptor outer segment Source: RGD
- sperm midpiece Source: RGD
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 92 | CytoplasmicSequence analysisAdd BLAST | 92 | |
Transmembranei | 93 – 113 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 114 – 137 | ExtracellularSequence analysisAdd BLAST | 24 | |
Transmembranei | 138 – 158 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 159 – 294 | CytoplasmicSequence analysisAdd BLAST | 136 | |
Transmembranei | 295 – 314 | HelicalSequence analysisAdd BLAST | 20 | |
Topological domaini | 315 – 326 | ExtracellularSequence analysisAdd BLAST | 12 | |
Transmembranei | 327 – 344 | HelicalSequence analysisAdd BLAST | 18 | |
Topological domaini | 345 – 777 | CytoplasmicSequence analysisAdd BLAST | 433 | |
Transmembranei | 778 – 797 | HelicalSequence analysisAdd BLAST | 20 | |
Topological domaini | 798 – 807 | ExtracellularSequence analysis | 10 | |
Transmembranei | 808 – 828 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 829 – 848 | CytoplasmicSequence analysisAdd BLAST | 20 | |
Transmembranei | 849 – 871 | HelicalSequence analysisAdd BLAST | 23 | |
Topological domaini | 872 – 923 | ExtracellularSequence analysisAdd BLAST | 52 | |
Transmembranei | 924 – 943 | HelicalSequence analysisAdd BLAST | 20 | |
Topological domaini | 944 – 956 | CytoplasmicSequence analysisAdd BLAST | 13 | |
Transmembranei | 957 – 975 | HelicalSequence analysisAdd BLAST | 19 | |
Topological domaini | 976 – 990 | ExtracellularSequence analysisAdd BLAST | 15 | |
Transmembranei | 991 – 1011 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 1012 – 1028 | CytoplasmicSequence analysisAdd BLAST | 17 |
Keywords - Cellular componenti
Cell membrane, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000046305 | 1 – 1028 | Sodium/potassium-transporting ATPase subunit alpha-4Add BLAST | 1028 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 948 | Phosphoserine; by PKABy similarity | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
jPOSTi | Q64541 |
PaxDbi | Q64541 |
PRIDEi | Q64541 |
PTM databases
iPTMneti | Q64541 |
PhosphoSitePlusi | Q64541 |
Expressioni
Gene expression databases
Bgeei | ENSRNOG00000032378, Expressed in testis and 18 other tissues |
Genevisiblei | Q64541, RN |
Interactioni
Subunit structurei
The sodium/potassium-transporting ATPase is composed of a catalytic alpha subunit, an auxiliary non-catalytic beta subunit and an additional regulatory subunit.
CuratedProtein-protein interaction databases
BioGRIDi | 247817, 1 interactor |
STRINGi | 10116.ENSRNOP00000062079 |
Chemistry databases
BindingDBi | Q64541 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 36 | DisorderedSequence analysisAdd BLAST | 36 | |
Regioni | 87 – 89 | Interaction with phosphoinositide-3 kinaseBy similarity | 3 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 11 – 25 | Polar residuesSequence analysisAdd BLAST | 15 |
Sequence similaritiesi
Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IIC subfamily. [View classification]Curated
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG0203, Eukaryota |
GeneTreei | ENSGT00940000162378 |
HOGENOMi | CLU_002360_4_3_1 |
InParanoidi | Q64541 |
OMAi | NSRVKFD |
OrthoDBi | 388324at2759 |
PhylomeDBi | Q64541 |
Family and domain databases
CDDi | cd02608, P-type_ATPase_Na-K_like, 1 hit |
Gene3Di | 3.40.1110.10, 1 hit 3.40.50.1000, 1 hit |
InterProi | View protein in InterPro IPR006068, ATPase_P-typ_cation-transptr_C IPR004014, ATPase_P-typ_cation-transptr_N IPR023299, ATPase_P-typ_cyto_dom_N IPR018303, ATPase_P-typ_P_site IPR023298, ATPase_P-typ_TM_dom_sf IPR008250, ATPase_P-typ_transduc_dom_A_sf IPR036412, HAD-like_sf IPR023214, HAD_sf IPR005775, P-type_ATPase_IIC IPR001757, P_typ_ATPase IPR044492, P_typ_ATPase_HD_dom |
Pfami | View protein in Pfam PF00689, Cation_ATPase_C, 1 hit PF00690, Cation_ATPase_N, 1 hit |
SFLDi | SFLDF00027, p-type_atpase, 1 hit |
SMARTi | View protein in SMART SM00831, Cation_ATPase_N, 1 hit |
SUPFAMi | SSF56784, SSF56784, 1 hit SSF81653, SSF81653, 1 hit SSF81660, SSF81660, 1 hit SSF81665, SSF81665, 1 hit |
TIGRFAMsi | TIGR01106, ATPase-IIC_X-K, 1 hit TIGR01494, ATPase_P-type, 2 hits |
PROSITEi | View protein in PROSITE PS00154, ATPASE_E1_E2, 1 hit |
i Sequence
Sequence statusi: Complete.
Q64541-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MEPGKETAAT SEQKPRPTLR ASNTNRQPKV KRRKKDLEEL KKEVVMDDHK
60 70 80 90 100
LTLDELSAKY SVDLTKGLSV TDAQEILTLN GPNVLTPPPT TPEWIKFCKQ
110 120 130 140 150
LFGGFSLLLW TGSLLCFLAY GIHVSYYQEN ANKDNLYLGI VLSAVVIITG
160 170 180 190 200
CFSYYQEAKS SKIMESFKTM VPQQALVIRD GEKMQINVRD VVLGDLVEVK
210 220 230 240 250
GGDQVPADIR VIAAQGCKVD NSSLTGESEP QSRCPDCTHE NPLETRNIIF
260 270 280 290 300
FSTNCVEGTA RGVVIATGDH TVMGRIASLT SGLTMGKTPI ATEIEHFIHI
310 320 330 340 350
ITAVAVFLGV TFFFLSLILG YTWLDAVIFL IGIIVANVPE GLLATVTVCL
360 370 380 390 400
TLTAKRMARK NCLVKNLEAV ETLGSTSTIC SDKTGTLTQN RMTVAHLWFD
410 420 430 440 450
KTVYEADTSE EQTTGKTFPK SSDTWFYLAR IAGLCNRADF KPHQESLPIT
460 470 480 490 500
KRTTTGDASE SALLKFIEQS YSPVSEMRQK NPKVAEIPFN STNKYQMSIH
510 520 530 540 550
LLEDNSEAHV LLMKGAPERI LDFCSSFLLN GQEYPMDEEM KTDFQNAYIE
560 570 580 590 600
LGGLGERVLG FCFLNLPSNF SKGFQFNTEE LNFPMENLCF AGLISMIDPP
610 620 630 640 650
RTAVPDAVSK CRSAGIKVIM VTGDHPITAK AIAKSVGIIS EANETAEDIA
660 670 680 690 700
ARLNISISQV SNKSIKAIVV HGSELKDMDS GQLDNILKSY KEIVFARTSP
710 720 730 740 750
QQKLIIVEGC QRLGAIVAVT GDGVNDSPAL KKADIGIAMG ITGSDVSKQA
760 770 780 790 800
ADMILLDDNF ASIVTGVEEG RLIFDNLKKS IAYTLTSNIP EITPFLLFIV
810 820 830 840 850
LSIPLPLGTI TILCIDLGTD MVPAISLAYE TPESDIMKRL PRNPKTDNLV
860 870 880 890 900
NDRLIGMAYG QIGMIQALAG FFTYFVILAE NGFKPLDLLG IRLYWDDTNL
910 920 930 940 950
NDLEDTYGQQ WTYEQRKVVE FTCQTAFFIS IVIVQWADLI ICKTRRNSLF
960 970 980 990 1000
KQGMKNKVLI FGLLEETILA ACLSYIPGMD VALRMYPLKI NWWFCALPYS
1010 1020
VLIFIYDEVR KLIIRRRPGG WLEKETYY
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U15176 mRNA Translation: AAB81285.1 |
RefSeqi | NP_001257959.1, NM_001271030.1 NP_074039.1, NM_022848.3 |
Genome annotation databases
GeneIDi | 29132 |
KEGGi | rno:29132 |
UCSCi | RGD:61952, rat |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U15176 mRNA Translation: AAB81285.1 |
RefSeqi | NP_001257959.1, NM_001271030.1 NP_074039.1, NM_022848.3 |
3D structure databases
AlphaFoldDBi | Q64541 |
SMRi | Q64541 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 247817, 1 interactor |
STRINGi | 10116.ENSRNOP00000062079 |
Chemistry databases
BindingDBi | Q64541 |
ChEMBLi | CHEMBL2485 |
PTM databases
iPTMneti | Q64541 |
PhosphoSitePlusi | Q64541 |
Proteomic databases
jPOSTi | Q64541 |
PaxDbi | Q64541 |
PRIDEi | Q64541 |
Genome annotation databases
GeneIDi | 29132 |
KEGGi | rno:29132 |
UCSCi | RGD:61952, rat |
Organism-specific databases
CTDi | 480 |
RGDi | 61952, Atp1a4 |
Phylogenomic databases
eggNOGi | KOG0203, Eukaryota |
GeneTreei | ENSGT00940000162378 |
HOGENOMi | CLU_002360_4_3_1 |
InParanoidi | Q64541 |
OMAi | NSRVKFD |
OrthoDBi | 388324at2759 |
PhylomeDBi | Q64541 |
Enzyme and pathway databases
Reactomei | R-RNO-5578775, Ion homeostasis R-RNO-936837, Ion transport by P-type ATPases |
SABIO-RKi | Q64541 |
Miscellaneous databases
PROi | PR:Q64541 |
Gene expression databases
Bgeei | ENSRNOG00000032378, Expressed in testis and 18 other tissues |
Genevisiblei | Q64541, RN |
Family and domain databases
CDDi | cd02608, P-type_ATPase_Na-K_like, 1 hit |
Gene3Di | 3.40.1110.10, 1 hit 3.40.50.1000, 1 hit |
InterProi | View protein in InterPro IPR006068, ATPase_P-typ_cation-transptr_C IPR004014, ATPase_P-typ_cation-transptr_N IPR023299, ATPase_P-typ_cyto_dom_N IPR018303, ATPase_P-typ_P_site IPR023298, ATPase_P-typ_TM_dom_sf IPR008250, ATPase_P-typ_transduc_dom_A_sf IPR036412, HAD-like_sf IPR023214, HAD_sf IPR005775, P-type_ATPase_IIC IPR001757, P_typ_ATPase IPR044492, P_typ_ATPase_HD_dom |
Pfami | View protein in Pfam PF00689, Cation_ATPase_C, 1 hit PF00690, Cation_ATPase_N, 1 hit |
SFLDi | SFLDF00027, p-type_atpase, 1 hit |
SMARTi | View protein in SMART SM00831, Cation_ATPase_N, 1 hit |
SUPFAMi | SSF56784, SSF56784, 1 hit SSF81653, SSF81653, 1 hit SSF81660, SSF81660, 1 hit SSF81665, SSF81665, 1 hit |
TIGRFAMsi | TIGR01106, ATPase-IIC_X-K, 1 hit TIGR01494, ATPase_P-type, 2 hits |
PROSITEi | View protein in PROSITE PS00154, ATPASE_E1_E2, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | AT1A4_RAT | |
Accessioni | Q64541Primary (citable) accession number: Q64541 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 18, 2001 |
Last sequence update: | November 1, 1996 | |
Last modified: | May 25, 2022 | |
This is version 166 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families