Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Protein Wnt-8a

Gene

Wnt8a

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Ligand for members of the frizzled family of seven transmembrane receptors. Plays a role in embryonic patterning.By similarity

GO - Molecular functioni

GO - Biological processi

  • canonical Wnt signaling pathway Source: MGI
  • cell fate commitment Source: GO_Central
  • cell morphogenesis Source: MGI
  • endoderm development Source: MGI
  • establishment of animal organ orientation Source: MGI
  • neuron differentiation Source: GO_Central
  • polarity specification of anterior/posterior axis Source: MGI
  • polarity specification of proximal/distal axis Source: MGI
  • regionalization Source: MGI
  • regulation of protein localization Source: MGI
  • secondary palate development Source: MGI
  • Wnt signaling pathway Source: GO_Central

Keywordsi

Molecular functionDevelopmental protein
Biological processWnt signaling pathway

Enzyme and pathway databases

ReactomeiR-MMU-3238698 WNT ligand biogenesis and trafficking
R-MMU-4641262 Disassembly of the destruction complex and recruitment of AXIN to the membrane

Names & Taxonomyi

Protein namesi
Recommended name:
Protein Wnt-8a
Alternative name(s):
Protein Wnt-8d
Stimulated by retinoic acid gene 11 protein
Gene namesi
Name:Wnt8a
Synonyms:Stra11, Wnt8d
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 18

Organism-specific databases

MGIiMGI:107924 Wnt8a

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Extracellular matrix, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 19Sequence analysisAdd BLAST19
ChainiPRO_000004144920 – 354Protein Wnt-8aAdd BLAST335

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi54 ↔ 65By similarity
Glycosylationi103N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi104 ↔ 112By similarity
Disulfide bondi114 ↔ 132By similarity
Disulfide bondi180 ↔ 194By similarity
Disulfide bondi182 ↔ 189By similarity
Lipidationi186O-palmitoleoyl serineBy similarity1
Disulfide bondi259 ↔ 297By similarity
Glycosylationi262N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi275 ↔ 290By similarity
Disulfide bondi294 ↔ 336By similarity
Disulfide bondi312 ↔ 327By similarity
Disulfide bondi314 ↔ 324By similarity
Disulfide bondi319 ↔ 320By similarity

Post-translational modificationi

Palmitoleoylation is required for efficient binding to frizzled receptors (By similarity). Depalmitoleoylation leads to Wnt signaling pathway inhibition (By similarity).By similarity
Proteolytic processing by TIKI1 and TIKI2 promotes oxidation and formation of large disulfide-bond oligomers, leading to inactivation of WNT8A.By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein, Lipoprotein

Proteomic databases

PaxDbiQ64527
PRIDEiQ64527

PTM databases

PhosphoSitePlusiQ64527

Expressioni

Developmental stagei

Expression in early stages of embryogenesis. Expression begins in the posterior region of early primitive streak-stage embryos and after it spreads into the embryonic ectoderm up to a sharp rostral boundary at the base of the developing headfolds. Expressed transiently in the newly formed mesoderm. Expression is down-regulated during somitogenesis. The expression is highly restricted during gastrulation and neurulation, both temporally and spatially.

Inductioni

By retinoic acid.1 Publication

Gene expression databases

BgeeiENSMUSG00000012282
CleanExiMM_WNT8A
GenevisibleiQ64527 MM

Interactioni

Subunit structurei

Forms a soluble 1:1 complex with AFM; this prevents oligomerization and is required for prolonged biological activity. The complex with AFM may represent the physiological form in body fluids.By similarity

GO - Molecular functioni

Protein-protein interaction databases

BioGridi203552, 1 interactor
STRINGi10090.ENSMUSP00000012426

Structurei

3D structure databases

ProteinModelPortaliQ64527
SMRiQ64527
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the Wnt family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG3913 Eukaryota
ENOG410XQZ1 LUCA
GeneTreeiENSGT00690000101857
HOGENOMiHOG000039529
HOVERGENiHBG001595
InParanoidiQ64527
KOiK00714
OMAiSAWSVNN
OrthoDBiEOG091G0B1B
TreeFamiTF105310

Family and domain databases

InterProiView protein in InterPro
IPR034312 Protein_Wnt-8A/8C
IPR005817 Wnt
IPR013301 Wnt8
IPR018161 Wnt_CS
PANTHERiPTHR12027 PTHR12027, 1 hit
PTHR12027:SF92 PTHR12027:SF92, 1 hit
PfamiView protein in Pfam
PF00110 wnt, 1 hit
PRINTSiPR01892 WNT8PROTEIN
PR01349 WNTPROTEIN
SMARTiView protein in SMART
SM00097 WNT1, 1 hit
PROSITEiView protein in PROSITE
PS00246 WNT1, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q64527-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGHLLMLWVA AGMCYPALGA SAWSVNNFLI TRPKAYLTYT ASVALGAQIG
60 70 80 90 100
IEECKFQFAW ERWNCPEHAF QFSTHNRLRA ATRETSFIHA IRSAAIMYAV
110 120 130 140 150
TKNCSMGDLE NCGCDESQNG KTGGHGWIWG GCSDNVEFGE KISRLFVDSL
160 170 180 190 200
EKGKDARALV NLHNNRAGRL AVRASTKRTC KCHGISGSCS IQTCWLQLAD
210 220 230 240 250
FRQMGNYLKA KYDRALKIEM DKRQLRAGNR AEGRWALTEA FLPSTEAELI
260 270 280 290 300
FLEGSPDYCN RNASLSIQGT EGRECLQNAR SASRREQRSC GRLCTECGLQ
310 320 330 340 350
VEERRAEAVS SCDCNFQWCC TVKCGQCRRV VSRYYCTRPV GSARPRGRGK

DSAW
Length:354
Mass (Da):39,573
Last modified:July 27, 2011 - v2
Checksum:iA91D5245F5B6FE65
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti32R → G in CAA93117 (PubMed:8887323).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z68889 mRNA Translation: CAA93117.1
AC074335 Genomic DNA No translation available.
CCDSiCCDS29129.1
RefSeqiNP_033316.1, NM_009290.2
UniGeneiMm.558

Genome annotation databases

EnsembliENSMUST00000012426; ENSMUSP00000012426; ENSMUSG00000012282
GeneIDi20890
KEGGimmu:20890

Similar proteinsi

Entry informationi

Entry nameiWNT8A_MOUSE
AccessioniPrimary (citable) accession number: Q64527
Secondary accession number(s): E9QL90
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: July 27, 2011
Last modified: June 20, 2018
This is version 135 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health