UniProtKB - Q64521 (GPDM_MOUSE)
Protein
Glycerol-3-phosphate dehydrogenase, mitochondrial
Gene
Gpd2
Organism
Mus musculus (Mouse)
Status
Functioni
Calcium-responsive mitochondrial glycerol-3-phosphate dehydrogenase which seems to be a key component of the pancreatic beta-cell glucose-sensing device.By similarity
Catalytic activityi
Cofactori
Activity regulationi
Calcium-binding enhance the activity of the enzyme.By similarity
: glycerol degradation via glycerol kinase pathway Pathwayi
This protein is involved in step 1 of the subpathway that synthesizes glycerone phosphate from sn-glycerol 3-phosphate (anaerobic route).Proteins known to be involved in this subpathway in this organism are:
- Glycerol-3-phosphate dehydrogenase, mitochondrial (Gpd2)
View all proteins of this organism that are known to be involved in the subpathway that synthesizes glycerone phosphate from sn-glycerol 3-phosphate (anaerobic route), the pathway glycerol degradation via glycerol kinase pathway and in Polyol metabolism.
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 71 – 99 | FADSequence analysisAdd BLAST | 29 | |
Calcium bindingi | 672 – 683 | PROSITE-ProRule annotationAdd BLAST | 12 |
GO - Molecular functioni
- calcium ion binding Source: InterPro
- glycerol-3-phosphate dehydrogenase [NAD+] activity Source: MGI
- sn-glycerol-3-phosphate:ubiquinone-8 oxidoreductase activity Source: UniProtKB-EC
GO - Biological processi
- camera-type eye development Source: MGI
- gluconeogenesis Source: MGI
- glycerol-3-phosphate metabolic process Source: MGI
- glycerol catabolic process Source: UniProtKB-UniPathway
- multicellular organism growth Source: MGI
- NADH metabolic process Source: MGI
Keywordsi
Molecular function | Oxidoreductase |
Ligand | Calcium, FAD, Flavoprotein, Metal-binding |
Enzyme and pathway databases
BRENDAi | 1.1.5.3, 3474 |
Reactomei | R-MMU-1483166, Synthesis of PA R-MMU-163560, Triglyceride catabolism |
UniPathwayi | UPA00618;UER00673 |
Names & Taxonomyi
Protein namesi | Recommended name: Glycerol-3-phosphate dehydrogenase, mitochondrial (EC:1.1.5.3By similarity)Short name: GPD-M Short name: GPDH-M Alternative name(s): Protein TISP38 |
Gene namesi | |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:99778, Gpd2 |
Subcellular locationi
Mitochondrion
Mitochondrion
- mitochondrial inner membrane Source: MGI
- mitochondrion Source: MGI
Other locations
- glycerol-3-phosphate dehydrogenase complex Source: MGI
Keywords - Cellular componenti
Membrane, Mitochondrion, Mitochondrion inner membranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transit peptidei | 1 – 42 | MitochondrionBy similarityAdd BLAST | 42 | |
ChainiPRO_0000010430 | 43 – 727 | Glycerol-3-phosphate dehydrogenase, mitochondrialAdd BLAST | 685 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 601 | PhosphotyrosineCombined sources | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
EPDi | Q64521 |
jPOSTi | Q64521 |
MaxQBi | Q64521 |
PaxDbi | Q64521 |
PeptideAtlasi | Q64521 |
PRIDEi | Q64521 |
ProteomicsDBi | 271263 |
2D gel databases
REPRODUCTION-2DPAGEi | Q64521 |
PTM databases
iPTMneti | Q64521 |
PhosphoSitePlusi | Q64521 |
SwissPalmi | Q64521 |
Expressioni
Gene expression databases
Bgeei | ENSMUSG00000026827, Expressed in brown adipose tissue and 330 other tissues |
ExpressionAtlasi | Q64521, baseline and differential |
Genevisiblei | Q64521, MM |
Interactioni
Protein-protein interaction databases
BioGRIDi | 199894, 18 interactors |
IntActi | Q64521, 5 interactors |
MINTi | Q64521 |
STRINGi | 10090.ENSMUSP00000108237 |
Miscellaneous databases
RNActi | Q64521, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 623 – 658 | EF-hand 1PROSITE-ProRule annotationAdd BLAST | 36 | |
Domaini | 659 – 694 | EF-hand 2PROSITE-ProRule annotationAdd BLAST | 36 |
Sequence similaritiesi
Belongs to the FAD-dependent glycerol-3-phosphate dehydrogenase family.Curated
Keywords - Domaini
Repeat, Transit peptidePhylogenomic databases
eggNOGi | KOG0042, Eukaryota |
GeneTreei | ENSGT00390000001718 |
InParanoidi | Q64521 |
OMAi | AHGFSKT |
OrthoDBi | 669193at2759 |
Family and domain databases
CDDi | cd00051, EFh, 1 hit |
Gene3Di | 1.10.8.870, 1 hit 3.50.50.60, 1 hit |
InterProi | View protein in InterPro IPR031656, DAO_C IPR038299, DAO_C_sf IPR011992, EF-hand-dom_pair IPR018247, EF_Hand_1_Ca_BS IPR002048, EF_hand_dom IPR006076, FAD-dep_OxRdtase IPR036188, FAD/NAD-bd_sf IPR000447, G3P_DH_FAD-dep |
PANTHERi | PTHR11985, PTHR11985, 1 hit |
Pfami | View protein in Pfam PF01266, DAO, 1 hit PF16901, DAO_C, 1 hit PF13499, EF-hand_7, 1 hit |
PRINTSi | PR01001, FADG3PDH |
SMARTi | View protein in SMART SM00054, EFh, 2 hits |
SUPFAMi | SSF47473, SSF47473, 1 hit SSF51905, SSF51905, 1 hit |
PROSITEi | View protein in PROSITE PS00018, EF_HAND_1, 1 hit PS50222, EF_HAND_2, 2 hits PS00977, FAD_G3PDH_1, 1 hit PS00978, FAD_G3PDH_2, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
Q64521-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MAFQKAVKGT ILVGGGALAT VLGLSPFAHY RRKQVSLAYV EAAGYLTEPV
60 70 80 90 100
NREPPSREAQ LMTLKNTPEF DILVIGGGAT GCGCALDAVT RGLKTALVER
110 120 130 140 150
DDFSSGTSSR STKLIHGGVR YLQKAIMNLD VEQYRMVKEA LHERANLLEI
160 170 180 190 200
APHLSAPLPI MLPLYKWWQL PYYWVGIKMY DLVAGSQCLK SSYVLSKSRA
210 220 230 240 250
LEHFPMLQKD KLVGAIVYYD GQHNDARMNL AIALTAARYG AATANYMEVV
260 270 280 290 300
SLLKKTDPET GKERVSGARC KDVLTGQEFD VRAKCVINAS GPFTDSVRKM
310 320 330 340 350
DDKNVVPICQ PSAGVHIVMP GYYSPENMGL LDPATSDGRV IFFLPWEKMT
360 370 380 390 400
IAGTTDTPTD VTHHPIPSEE DINFILNEVR NYLSSDVEVR RGDVLAAWSG
410 420 430 440 450
IRPLVTDPKS ADTQSISRNH VVDISDSGLI TIAGGKWTTY RSMAEDTVDA
460 470 480 490 500
AVKFHNLNAG PSRTVGLFLQ GGKDWSPTLY IRLVQDYGLE SEVAQHLAKT
510 520 530 540 550
YGDKAFEVAK MASVTGKRWP VVGVRLVSEF PYIEAEVKYG IKEYACTAVD
560 570 580 590 600
MISRRTRLAF LNVQAAEEAL PRIVELMGRE LNWSELRKQE ELETATRFLY
610 620 630 640 650
YEMGYKSRTE QLTDSTEISL LPSDIDRYKK RFHKFDEDEK GFITIVDVQR
660 670 680 690 700
VLESINVQMD ENTLHEILCE VDLNKNGQVE LHEFLQLMSA VQKGRVSGSR
710 720
LAILMKTAEE NLDRRVPIPV DRSCGGL
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketA2AQR0 | A2AQR0_MOUSE | Glycerol-3-phosphate dehydrogenase | Gpd2 | 745 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 26 | P → Q in AAB50545 (PubMed:8951039).Curated | 1 | |
Sequence conflicti | 26 | P → Q in AAH21359 (PubMed:15489334).Curated | 1 | |
Sequence conflicti | 42 | Missing in BAC28685 (PubMed:16141072).Curated | 1 | |
Sequence conflicti | 158 | L → V in AAB50545 (PubMed:8951039).Curated | 1 | |
Sequence conflicti | 295 | D → E in BAA08926 (PubMed:8772729).Curated | 1 | |
Sequence conflicti | 449 | D → N in AAH21359 (PubMed:15489334).Curated | 1 | |
Sequence conflicti | 498 | A → P in BAA08926 (PubMed:8772729).Curated | 1 | |
Sequence conflicti | 582 | N → D in AAB50545 (PubMed:8951039).Curated | 1 | |
Sequence conflicti | 593 | E → G in AAB50545 (PubMed:8951039).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U60987 mRNA Translation: AAB50545.1 D50430 mRNA Translation: BAA08926.1 AK034353 mRNA Translation: BAC28685.1 AK144716 mRNA Translation: BAE26028.1 AK149851 mRNA Translation: BAE29123.1 AK167152 mRNA Translation: BAE39294.1 BC021359 mRNA Translation: AAH21359.1 AH009802 Genomic DNA Translation: AAG12342.1 AB045714 mRNA Translation: BAB97201.1 |
CCDSi | CCDS16045.1 |
RefSeqi | NP_001139292.1, NM_001145820.1 NP_034404.3, NM_010274.3 |
Genome annotation databases
Ensembli | ENSMUST00000028167; ENSMUSP00000028167; ENSMUSG00000026827 ENSMUST00000169687; ENSMUSP00000130992; ENSMUSG00000026827 |
GeneIDi | 14571 |
KEGGi | mmu:14571 |
UCSCi | uc008jsc.2, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U60987 mRNA Translation: AAB50545.1 D50430 mRNA Translation: BAA08926.1 AK034353 mRNA Translation: BAC28685.1 AK144716 mRNA Translation: BAE26028.1 AK149851 mRNA Translation: BAE29123.1 AK167152 mRNA Translation: BAE39294.1 BC021359 mRNA Translation: AAH21359.1 AH009802 Genomic DNA Translation: AAG12342.1 AB045714 mRNA Translation: BAB97201.1 |
CCDSi | CCDS16045.1 |
RefSeqi | NP_001139292.1, NM_001145820.1 NP_034404.3, NM_010274.3 |
3D structure databases
SMRi | Q64521 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 199894, 18 interactors |
IntActi | Q64521, 5 interactors |
MINTi | Q64521 |
STRINGi | 10090.ENSMUSP00000108237 |
PTM databases
iPTMneti | Q64521 |
PhosphoSitePlusi | Q64521 |
SwissPalmi | Q64521 |
2D gel databases
REPRODUCTION-2DPAGEi | Q64521 |
Proteomic databases
EPDi | Q64521 |
jPOSTi | Q64521 |
MaxQBi | Q64521 |
PaxDbi | Q64521 |
PeptideAtlasi | Q64521 |
PRIDEi | Q64521 |
ProteomicsDBi | 271263 |
Protocols and materials databases
Antibodypediai | 2370, 270 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000028167; ENSMUSP00000028167; ENSMUSG00000026827 ENSMUST00000169687; ENSMUSP00000130992; ENSMUSG00000026827 |
GeneIDi | 14571 |
KEGGi | mmu:14571 |
UCSCi | uc008jsc.2, mouse |
Organism-specific databases
CTDi | 2820 |
MGIi | MGI:99778, Gpd2 |
Phylogenomic databases
eggNOGi | KOG0042, Eukaryota |
GeneTreei | ENSGT00390000001718 |
InParanoidi | Q64521 |
OMAi | AHGFSKT |
OrthoDBi | 669193at2759 |
Enzyme and pathway databases
UniPathwayi | UPA00618;UER00673 |
BRENDAi | 1.1.5.3, 3474 |
Reactomei | R-MMU-1483166, Synthesis of PA R-MMU-163560, Triglyceride catabolism |
Miscellaneous databases
BioGRID-ORCSi | 14571, 0 hits in 53 CRISPR screens |
ChiTaRSi | Gpd2, mouse |
PROi | PR:Q64521 |
RNActi | Q64521, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000026827, Expressed in brown adipose tissue and 330 other tissues |
ExpressionAtlasi | Q64521, baseline and differential |
Genevisiblei | Q64521, MM |
Family and domain databases
CDDi | cd00051, EFh, 1 hit |
Gene3Di | 1.10.8.870, 1 hit 3.50.50.60, 1 hit |
InterProi | View protein in InterPro IPR031656, DAO_C IPR038299, DAO_C_sf IPR011992, EF-hand-dom_pair IPR018247, EF_Hand_1_Ca_BS IPR002048, EF_hand_dom IPR006076, FAD-dep_OxRdtase IPR036188, FAD/NAD-bd_sf IPR000447, G3P_DH_FAD-dep |
PANTHERi | PTHR11985, PTHR11985, 1 hit |
Pfami | View protein in Pfam PF01266, DAO, 1 hit PF16901, DAO_C, 1 hit PF13499, EF-hand_7, 1 hit |
PRINTSi | PR01001, FADG3PDH |
SMARTi | View protein in SMART SM00054, EFh, 2 hits |
SUPFAMi | SSF47473, SSF47473, 1 hit SSF51905, SSF51905, 1 hit |
PROSITEi | View protein in PROSITE PS00018, EF_HAND_1, 1 hit PS50222, EF_HAND_2, 2 hits PS00977, FAD_G3PDH_1, 1 hit PS00978, FAD_G3PDH_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | GPDM_MOUSE | |
Accessioni | Q64521Primary (citable) accession number: Q64521 Secondary accession number(s): Q3TK51 Q9ERP0 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 1, 1997 |
Last sequence update: | May 1, 2007 | |
Last modified: | April 7, 2021 | |
This is version 176 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Direct protein sequencing, Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families