UniProtKB - Q64444 (CAH4_MOUSE)
Carbonic anhydrase 4
Ca4
Functioni
Catalyzes the reversible hydration of carbon dioxide into bicarbonate and protons and thus is essential to maintaining intracellular and extracellular pH. May stimulate the sodium/bicarbonate transporter activity of SLC4A4 that acts in pH homeostasis. It is essential for acid overload removal from the retina and retina epithelium, and acid release in the choriocapillaris in the choroid.
By similarityCatalytic activityi
- EC:4.2.1.1By similarity
Cofactori
Activity regulationi
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 87 | Proton donor/acceptorBy similarity | 1 | |
Metal bindingi | 114 | Zinc; catalytic1 Publication | 1 | |
Metal bindingi | 116 | Zinc; catalytic1 Publication | 1 | |
Metal bindingi | 139 | Zinc; catalytic1 Publication | 1 |
GO - Molecular functioni
- carbonate dehydratase activity Source: MGI
- hydro-lyase activity Source: GO_Central
- zinc ion binding Source: InterPro
GO - Biological processi
- bicarbonate transport Source: MGI
- carbon dioxide transport Source: MGI
- one-carbon metabolic process Source: GO_Central
- regulation of pH Source: MGI
Keywordsi
Molecular function | Lyase |
Ligand | Metal-binding, Zinc |
Enzyme and pathway databases
Reactomei | R-MMU-1237044, Erythrocytes take up carbon dioxide and release oxygen R-MMU-1247673, Erythrocytes take up oxygen and release carbon dioxide R-MMU-1475029, Reversible hydration of carbon dioxide |
Names & Taxonomyi
Protein namesi | Recommended name: Carbonic anhydrase 4 (EC:4.2.1.1By similarity)Alternative name(s): Carbonate dehydratase IV Carbonic anhydrase IV Short name: CA-IV |
Gene namesi | Name:Ca4 Synonyms:Car4 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1096574, Car4 |
VEuPathDBi | HostDB:ENSMUSG00000000805 |
Subcellular locationi
Plasma membrane
- Cell membrane By similarity; GPI-anchor By similarity
Endoplasmic reticulum
- rough endoplasmic reticulum Source: MGI
- sarcoplasmic reticulum Source: MGI
Extracellular region or secreted
- extracellular exosome Source: MGI
Golgi apparatus
- Golgi apparatus Source: MGI
- trans-Golgi network Source: MGI
Plasma Membrane
- anchored component of external side of plasma membrane Source: MGI
- anchored component of plasma membrane Source: MGI
- apical plasma membrane Source: MGI
- brush border membrane Source: MGI
- plasma membrane Source: MGI
- sarcolemma Source: MGI
Other locations
- cell surface Source: MGI
- endoplasmic reticulum-Golgi intermediate compartment Source: MGI
- membrane Source: MGI
- perinuclear region of cytoplasm Source: MGI
- secretory granule membrane Source: MGI
- transport vesicle membrane Source: MGI
Keywords - Cellular componenti
Cell membrane, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 17 | Sequence analysisAdd BLAST | 17 | |
ChainiPRO_0000004228 | 18 – 277 | Carbonic anhydrase 4Add BLAST | 260 | |
PropeptideiPRO_0000004229 | 278 – 305 | Removed in mature formBy similarityAdd BLAST | 28 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Disulfide bondi | 23 ↔ 35 | 1 Publication | ||
Disulfide bondi | 45 ↔ 222 | 1 Publication | ||
Glycosylationi | 123 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 214 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Lipidationi | 277 | GPI-anchor amidated serineBy similarity | 1 |
Keywords - PTMi
Disulfide bond, Glycoprotein, GPI-anchor, LipoproteinProteomic databases
jPOSTi | Q64444 |
MaxQBi | Q64444 |
PaxDbi | Q64444 |
PeptideAtlasi | Q64444 |
PRIDEi | Q64444 |
ProteomicsDBi | 265422 |
PTM databases
GlyConnecti | 2181, 4 N-Linked glycans (2 sites) |
GlyGeni | Q64444, 2 sites, 4 N-linked glycans (2 sites) |
PhosphoSitePlusi | Q64444 |
Expressioni
Gene expression databases
Bgeei | ENSMUSG00000000805, Expressed in left colon and 254 other tissues |
ExpressionAtlasi | Q64444, baseline and differential |
Genevisiblei | Q64444, MM |
Interactioni
Subunit structurei
Interacts with SLC4A4.
1 PublicationBinary interactionsi
Q64444
With | #Exp. | IntAct |
---|---|---|
Slc4a4 [O88343] | 2 | EBI-6859308,EBI-771342 |
Protein-protein interaction databases
IntActi | Q64444, 1 interactor |
STRINGi | 10090.ENSMUSP00000099483 |
Miscellaneous databases
RNActi | Q64444, protein |
Structurei
Secondary structure
3D structure databases
AlphaFoldDBi | Q64444 |
SMRi | Q64444 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | Q64444 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 20 – 278 | Alpha-carbonic anhydrasePROSITE-ProRule annotationAdd BLAST | 259 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 218 – 219 | Substrate bindingBy similarity | 2 |
Sequence similaritiesi
Keywords - Domaini
SignalPhylogenomic databases
eggNOGi | KOG0382, Eukaryota |
GeneTreei | ENSGT00940000155690 |
HOGENOMi | CLU_039326_2_0_1 |
InParanoidi | Q64444 |
OMAi | SHWCYEI |
OrthoDBi | 1377476at2759 |
PhylomeDBi | Q64444 |
TreeFami | TF316425 |
Family and domain databases
CDDi | cd03117, alpha_CA_IV_XV_like, 1 hit |
Gene3Di | 3.10.200.10, 1 hit |
InterProi | View protein in InterPro IPR041874, CA4/CA15 IPR001148, CA_dom IPR036398, CA_dom_sf IPR023561, Carbonic_anhydrase_a-class IPR018338, Carbonic_anhydrase_a-class_CS IPR018343, Carbonic_anhydrase_CA4 |
PANTHERi | PTHR18952, PTHR18952, 1 hit PTHR18952:SF95, PTHR18952:SF95, 1 hit |
Pfami | View protein in Pfam PF00194, Carb_anhydrase, 1 hit |
SMARTi | View protein in SMART SM01057, Carb_anhydrase, 1 hit |
SUPFAMi | SSF51069, SSF51069, 1 hit |
PROSITEi | View protein in PROSITE PS00162, ALPHA_CA_1, 1 hit PS51144, ALPHA_CA_2, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry has 1 described isoform and 2 potential isoforms that are computationally mapped.Show allAlign All
10 20 30 40 50
MQLLLALLAL AYVAPSTEDS GWCYEIQTKD PRSSCLGPEK WPGACKENQQ
60 70 80 90 100
SPINIVTART KVNPRLTPFI LVGYDQKQQW PIKNNQHTVE MTLGGGACII
110 120 130 140 150
GGDLPARYEA VQLHLHWSNG NDNGSEHSID GRHFAMEMHI VHKKLTSSKE
160 170 180 190 200
DSKDKFAVLA FMIEVGDKVN KGFQPLVEAL PSISKPHSTS TVRESSLQDM
210 220 230 240 250
LPPSTKMYTY FRYNGSLTTP NCDETVIWTV YKQPIKIHKN QFLEFSKNLY
260 270 280 290 300
YDEDQKLNMK DNVRPLQPLG KRQVFKSHAP GQLLSLPLPT LLVPTLTCLV
ANFLQ
Computationally mapped potential isoform sequencesi
There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketF6ST32 | F6ST32_MOUSE | Carbonic anhydrase 4 | Car4 | 164 | Annotation score: | ||
D6RCZ3 | D6RCZ3_MOUSE | Carbonic anhydrase 4 | Car4 | 38 | Annotation score: |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U37091 Genomic DNA Translation: AAC52569.1 BC012704 mRNA Translation: AAH12704.1 |
CCDSi | CCDS25190.1 |
RefSeqi | NP_031633.1, NM_007607.2 |
Genome annotation databases
Ensembli | ENSMUST00000103194; ENSMUSP00000099483; ENSMUSG00000000805 |
GeneIDi | 12351 |
KEGGi | mmu:12351 |
UCSCi | uc007krg.2, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U37091 Genomic DNA Translation: AAC52569.1 BC012704 mRNA Translation: AAH12704.1 |
CCDSi | CCDS25190.1 |
RefSeqi | NP_031633.1, NM_007607.2 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
2ZNC | X-ray | 2.80 | A | 22-279 | [»] | |
3ZNC | X-ray | 2.80 | A | 22-279 | [»] | |
AlphaFoldDBi | Q64444 | |||||
SMRi | Q64444 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
IntActi | Q64444, 1 interactor |
STRINGi | 10090.ENSMUSP00000099483 |
PTM databases
GlyConnecti | 2181, 4 N-Linked glycans (2 sites) |
GlyGeni | Q64444, 2 sites, 4 N-linked glycans (2 sites) |
PhosphoSitePlusi | Q64444 |
Proteomic databases
jPOSTi | Q64444 |
MaxQBi | Q64444 |
PaxDbi | Q64444 |
PeptideAtlasi | Q64444 |
PRIDEi | Q64444 |
ProteomicsDBi | 265422 |
Protocols and materials databases
Antibodypediai | 2592, 319 antibodies from 33 providers |
DNASUi | 12351 |
Genome annotation databases
Ensembli | ENSMUST00000103194; ENSMUSP00000099483; ENSMUSG00000000805 |
GeneIDi | 12351 |
KEGGi | mmu:12351 |
UCSCi | uc007krg.2, mouse |
Organism-specific databases
CTDi | 12351 |
MGIi | MGI:1096574, Car4 |
VEuPathDBi | HostDB:ENSMUSG00000000805 |
Phylogenomic databases
eggNOGi | KOG0382, Eukaryota |
GeneTreei | ENSGT00940000155690 |
HOGENOMi | CLU_039326_2_0_1 |
InParanoidi | Q64444 |
OMAi | SHWCYEI |
OrthoDBi | 1377476at2759 |
PhylomeDBi | Q64444 |
TreeFami | TF316425 |
Enzyme and pathway databases
Reactomei | R-MMU-1237044, Erythrocytes take up carbon dioxide and release oxygen R-MMU-1247673, Erythrocytes take up oxygen and release carbon dioxide R-MMU-1475029, Reversible hydration of carbon dioxide |
Miscellaneous databases
BioGRID-ORCSi | 12351, 2 hits in 74 CRISPR screens |
ChiTaRSi | Car4, mouse |
EvolutionaryTracei | Q64444 |
PROi | PR:Q64444 |
RNActi | Q64444, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000000805, Expressed in left colon and 254 other tissues |
ExpressionAtlasi | Q64444, baseline and differential |
Genevisiblei | Q64444, MM |
Family and domain databases
CDDi | cd03117, alpha_CA_IV_XV_like, 1 hit |
Gene3Di | 3.10.200.10, 1 hit |
InterProi | View protein in InterPro IPR041874, CA4/CA15 IPR001148, CA_dom IPR036398, CA_dom_sf IPR023561, Carbonic_anhydrase_a-class IPR018338, Carbonic_anhydrase_a-class_CS IPR018343, Carbonic_anhydrase_CA4 |
PANTHERi | PTHR18952, PTHR18952, 1 hit PTHR18952:SF95, PTHR18952:SF95, 1 hit |
Pfami | View protein in Pfam PF00194, Carb_anhydrase, 1 hit |
SMARTi | View protein in SMART SM01057, Carb_anhydrase, 1 hit |
SUPFAMi | SSF51069, SSF51069, 1 hit |
PROSITEi | View protein in PROSITE PS00162, ALPHA_CA_1, 1 hit PS51144, ALPHA_CA_2, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | CAH4_MOUSE | |
Accessioni | Q64444Primary (citable) accession number: Q64444 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 1, 1997 |
Last sequence update: | November 1, 1997 | |
Last modified: | May 25, 2022 | |
This is version 171 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families