UniProtKB - Q64444 (CAH4_MOUSE)

BLAST

>sp|Q64444|CAH4_MOUSE Carbonic anhydrase 4 OS=Mus musculus OX=10090 GN=Ca4 PE=1 SV=1
MQLLLALLALAYVAPSTEDSGWCYEIQTKDPRSSCLGPEKWPGACKENQQSPINIVTART
KVNPRLTPFILVGYDQKQQWPIKNNQHTVEMTLGGGACIIGGDLPARYEAVQLHLHWSNG
NDNGSEHSIDGRHFAMEMHIVHKKLTSSKEDSKDKFAVLAFMIEVGDKVNKGFQPLVEAL
PSISKPHSTSTVRESSLQDMLPPSTKMYTYFRYNGSLTTPNCDETVIWTVYKQPIKIHKN
QFLEFSKNLYYDEDQKLNMKDNVRPLQPLGKRQVFKSHAPGQLLSLPLPTLLVPTLTCLV
ANFLQ

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History
Entry version 152 (07 Nov 2018)
Sequence version 1 (01 Nov 1997)
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Protein

Carbonic anhydrase 4

Gene

Ca4

Organism

Mus musculus (Mouse)

Status

Reviewed-Annotation score: -Experimental evidence at protein levelⁱ

Functionⁱ

Reversible hydration of carbon dioxide. May stimulate the sodium/bicarbonate transporter activity of SLC4A4 (By similarity).By similarity

Catalytic activityⁱ

H₂CO₃ = CO₂ + H₂O.

Cofactorⁱ

Zn²⁺1 Publication

Activity regulationⁱ

Inhibited by acetazolamide.By similarity

Sites

Feature key	Position(s)	DescriptionActions	Length
Active siteⁱ	87	Proton acceptorBy similarity	1
Metal bindingⁱ	114	Zinc; catalytic1 Publication	1
Metal bindingⁱ	116	Zinc; catalytic1 Publication	1
Metal bindingⁱ	139	Zinc; catalytic1 Publication	1

GO - Molecular functionⁱ

carbonate dehydratase activity
Source: MGI
Inferred from direct assayⁱ
- 14660577
- 16083424
zinc ion binding Source: InterPro

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

bicarbonate transport Source: MGI
carbon dioxide transport
Source: MGI
Inferred by curatorⁱ
- 14660577
regulation of pH
Source: MGI
Inferred by curatorⁱ
- 14660577

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Molecular function	Lyase
Ligand	Metal-binding, Zinc

Enzyme and pathway databases

Reactomeⁱ	R-MMU-1237044 Erythrocytes take up carbon dioxide and release oxygen R-MMU-1247673 Erythrocytes take up oxygen and release carbon dioxide R-MMU-1475029 Reversible hydration of carbon dioxide

Reactomeⁱ

R-MMU-1237044 Erythrocytes take up carbon dioxide and release oxygen
R-MMU-1247673 Erythrocytes take up oxygen and release carbon dioxide
R-MMU-1475029 Reversible hydration of carbon dioxide

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: Carbonic anhydrase 4 (EC:4.2.1.1) Alternative name(s): Carbonate dehydratase IV Carbonic anhydrase IV Short name: CA-IV
Gene namesⁱ	Name:Ca4 Synonyms:Car4
Organismⁱ	Mus musculus (Mouse)
Taxonomic identifierⁱ	10090 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Dipnotetrapodomorpha › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Boreoeutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus
Proteomesⁱ	UP000000589 Componentⁱ: Chromosome 11

Organism-specific databases

MGIⁱ	MGI:1096574 Car4

Keywords - Cellular componentⁱ

Cell membrane, Membrane

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Length
Signal peptideⁱ	1 – 17	Sequence analysisAdd BLAST	17
ChainⁱPRO_0000004228	18 – 277	Carbonic anhydrase 4Add BLAST	260
PropeptideⁱPRO_0000004229	278 – 305	Removed in mature formSequence analysisAdd BLAST	28

Amino acid modifications

Feature key	Position(s)	DescriptionActions	Length
Disulfide bondⁱ	23 ↔ 35	1 Publication
Disulfide bondⁱ	45 ↔ 222	1 Publication
Glycosylationⁱ	123	N-linked (GlcNAc...) asparagineSequence analysis	1
Glycosylationⁱ	214	N-linked (GlcNAc...) asparagineSequence analysis	1
Lipidationⁱ	277	GPI-anchor amidated serineBy similarity	1

Keywords - PTMⁱ

Disulfide bond, Glycoprotein, GPI-anchor, Lipoprotein

Proteomic databases

MaxQB - The MaxQuant DataBase More...MaxQBⁱ	Q64444
PaxDbⁱ	Q64444
PeptideAtlas More...PeptideAtlasⁱ	Q64444
PRIDEⁱ	Q64444

PTM databases

PhosphoSitePlusⁱ	Q64444

PhosphoSitePlusⁱ

Q64444

Expressionⁱ

Gene expression databases

Bgeeⁱ	ENSMUSG00000000805 Expressed in 226 organ(s), highest expression level in left colon
CleanExⁱ	MM_CAR4
ExpressionAtlasⁱ	Q64444 baseline and differential
Genevisibleⁱ	Q64444 MM

Interactionⁱ

Subunit structureⁱ

Binary interactionsⁱ

With	Entry	#Exp.	IntAct	Notes
Slc4a4	O88343	2	EBI-6859308,EBI-771342

With

Entry

#Exp.

IntAct

Notes

Slc4a4

O88343

EBI-6859308,EBI-771342

Protein-protein interaction databases

IntActⁱ	Q64444, 2 interactors
Molecular INTeraction database More...MINTⁱ	Q64444
STRINGⁱ	10090.ENSMUSP00000099483

Structureⁱ

Secondary structure

Legend: HelixTurnBeta strandPDB Structure known for this area

Feature key	Position(s)	DescriptionActions	Length
Helixⁱ	25 – 29	Combined sources	5
Beta strandⁱ	30 – 33	Combined sources	4
Helixⁱ	38 – 40	Combined sources	3
Helixⁱ	43 – 46	Combined sources	4
Beta strandⁱ	47 – 49	Combined sources	3
Helixⁱ	57 – 59	Combined sources	3
Beta strandⁱ	70 – 73	Combined sources	4
Beta strandⁱ	78 – 84	Combined sources	7
Beta strandⁱ	86 – 92	Combined sources	7
Beta strandⁱ	96 – 100	Combined sources	5
Beta strandⁱ	108 – 117	Combined sources	10
Beta strandⁱ	119 – 123	Combined sources	5
Beta strandⁱ	125 – 129	Combined sources	5
Beta strandⁱ	135 – 144	Combined sources	10
Beta strandⁱ	156 – 168	Combined sources	13
Helixⁱ	171 – 173	Combined sources	3
Helixⁱ	174 – 179	Combined sources	6
Helixⁱ	180 – 182	Combined sources	3
Beta strandⁱ	189 – 195	Combined sources	7
Helixⁱ	198 – 200	Combined sources	3
Helixⁱ	204 – 206	Combined sources	3
Beta strandⁱ	210 – 215	Combined sources	6
Beta strandⁱ	226 – 233	Combined sources	8
Beta strandⁱ	235 – 238	Combined sources	4
Helixⁱ	239 – 248	Combined sources	10
Beta strandⁱ	250 – 252	Combined sources	3
Beta strandⁱ	275 – 277	Combined sources	3

Show more details

3D structure databases

ProteinModelPortalⁱ	Q64444
SMRⁱ	Q64444
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Miscellaneous databases

EvolutionaryTraceⁱ

Q64444

Family & Domainsⁱ

Domains and Repeats

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Domainⁱ	20 – 278	Alpha-carbonic anhydrasePROSITE-ProRule annotationAdd BLAST		259

Region

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Regionⁱ	218 – 219	Substrate bindingBy similarity		2

Sequence similaritiesⁱ

Belongs to the alpha-carbonic anhydrase family.Curated

Keywords - Domainⁱ

Signal

Phylogenomic databases

eggNOGⁱ	KOG0382 Eukaryota COG3338 LUCA
Ensembl GeneTree More...GeneTreeⁱ	ENSGT00760000118915
HOGENOMⁱ	HOG000112637
HOVERGENⁱ	HBG002837
InParanoidⁱ	Q64444
KEGG Orthology (KO) More...KOⁱ	K18246
OMAⁱ	ESHWCYE
Database of Orthologous Groups More...OrthoDBⁱ	EOG091G0XFM
TreeFamⁱ	TF316425

Family and domain databases

Gene3Dⁱ	3.10.200.10, 1 hit
InterProⁱ	View protein in InterPro IPR001148 CA_dom IPR036398 CA_dom_sf IPR023561 Carbonic_anhydrase_a-class IPR018338 Carbonic_anhydrase_a-class_CS IPR018343 Carbonic_anhydrase_CA4
PANTHERⁱ	PTHR18952 PTHR18952, 1 hit PTHR18952:SF95 PTHR18952:SF95, 1 hit
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF00194 Carb_anhydrase, 1 hit
SMARTⁱ	View protein in SMART SM01057 Carb_anhydrase, 1 hit
SUPFAMⁱ	SSF51069 SSF51069, 1 hit
PROSITEⁱ	View protein in PROSITE PS00162 ALPHA_CA_1, 1 hit PS51144 ALPHA_CA_2, 1 hit

Sequence (1+)ⁱ

Sequence statusⁱ: Complete.

Sequence processingⁱ: The displayed sequence is further processed into a mature form.

This entry has 1 described isoform and 2 potential isoforms that are computationally mapped.Show all Align All

Q64444-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
MQLLLALLAL AYVAPSTEDS GWCYEIQTKD PRSSCLGPEK WPGACKENQQ 
        60         70         80         90        100
SPINIVTART KVNPRLTPFI LVGYDQKQQW PIKNNQHTVE MTLGGGACII 
       110        120        130        140        150
GGDLPARYEA VQLHLHWSNG NDNGSEHSID GRHFAMEMHI VHKKLTSSKE 
       160        170        180        190        200
DSKDKFAVLA FMIEVGDKVN KGFQPLVEAL PSISKPHSTS TVRESSLQDM 
       210        220        230        240        250
LPPSTKMYTY FRYNGSLTTP NCDETVIWTV YKQPIKIHKN QFLEFSKNLY 
       260        270        280        290        300
YDEDQKLNMK DNVRPLQPLG KRQVFKSHAP GQLLSLPLPT LLVPTLTCLV 

ANFLQ

Length:305

Mass (Da):34,351

Last modified:November 1, 1997 - v1

Checksum:ⁱEEE988FF52732884

Computationally mapped potential isoform sequencesⁱ

There are 2 potential isoforms mapped to this entry.BLAST Align Show all Add to basket

Entry	Entry name	Protein names	Gene names	Length	Annotation

F6ST32	F6ST32_MOUSE	Carbonic anhydrase 4 Carbonic anhydrase 4	Car4	164	Annotation score:
D6RCZ3	D6RCZ3_MOUSE	Carbonic anhydrase 4 Carbonic anhydrase 4	Car4	38	Annotation score:

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	U37091 Genomic DNA Translation: AAC52569.1 BC012704 mRNA Translation: AAH12704.1
The Consensus CDS (CCDS) project More...CCDSⁱ	CCDS25190.1
NCBI Reference Sequences More...RefSeqⁱ	NP_031633.1, NM_007607.2
UniGeneⁱ	Mm.1641

Genome annotation databases

Ensemblⁱ	ENSMUST00000103194; ENSMUSP00000099483; ENSMUSG00000000805
GeneIDⁱ	12351
KEGGⁱ	mmu:12351
UCSC genome browser More...UCSCⁱ	uc007krg.2 mouse

Similar proteinsⁱ

50% Identity

Protein	Similar proteins		Species	Score	Length	Source
Q64444	Carbonic anhydrase 4		BOVIN		312	UniRef50_Q64444
Carbonic anhydrase 4		RAT		309
Carbonic anhydrase 4		HETGA		307
carbonic anhydrase 4		PHYCD		312
carbonic anhydrase 4		DIPOR		305
+85

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	U37091 Genomic DNA Translation: AAC52569.1 BC012704 mRNA Translation: AAH12704.1
CCDSⁱ	CCDS25190.1
RefSeqⁱ	NP_031633.1, NM_007607.2
UniGeneⁱ	Mm.1641

3D structure databases

Select the link destinations: Protein Data Bank Europe More...PDBeⁱ Protein Data Bank RCSB More...RCSB PDBⁱ Protein Data Bank Japan More...PDBjⁱ Links Updated	PDB entry	Method	Resolution (Å)	Chain	Positions	PDBsum
	2ZNC	X-ray	2.80	A	22-279	[»]
	3ZNC	X-ray	2.80	A	22-279	[»]
ProteinModelPortalⁱ	Q64444
SMRⁱ	Q64444
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

IntActⁱ	Q64444, 2 interactors
MINTⁱ	Q64444
STRINGⁱ	10090.ENSMUSP00000099483

PTM databases

PhosphoSitePlusⁱ	Q64444

PhosphoSitePlusⁱ

Q64444

Proteomic databases

MaxQBⁱ	Q64444
PaxDbⁱ	Q64444
PeptideAtlasⁱ	Q64444
PRIDEⁱ	Q64444

Protocols and materials databases

Structural Biology Knowledgebase	Search...

Structural Biology Knowledgebase

Search...

Genome annotation databases

Ensemblⁱ	ENSMUST00000103194; ENSMUSP00000099483; ENSMUSG00000000805
GeneIDⁱ	12351
KEGGⁱ	mmu:12351
UCSCⁱ	uc007krg.2 mouse

Organism-specific databases

CTDⁱ	12351
MGIⁱ	MGI:1096574 Car4

Phylogenomic databases

eggNOGⁱ	KOG0382 Eukaryota COG3338 LUCA
GeneTreeⁱ	ENSGT00760000118915
HOGENOMⁱ	HOG000112637
HOVERGENⁱ	HBG002837
InParanoidⁱ	Q64444
KOⁱ	K18246
OMAⁱ	ESHWCYE
OrthoDBⁱ	EOG091G0XFM
TreeFamⁱ	TF316425

Enzyme and pathway databases

Reactomeⁱ	R-MMU-1237044 Erythrocytes take up carbon dioxide and release oxygen R-MMU-1247673 Erythrocytes take up oxygen and release carbon dioxide R-MMU-1475029 Reversible hydration of carbon dioxide

Reactomeⁱ

R-MMU-1237044 Erythrocytes take up carbon dioxide and release oxygen
R-MMU-1247673 Erythrocytes take up oxygen and release carbon dioxide
R-MMU-1475029 Reversible hydration of carbon dioxide

Miscellaneous databases

EvolutionaryTraceⁱ	Q64444
Protein Ontology More...PROⁱ	PR:Q64444
SOURCEⁱ	Search...

Gene expression databases

Bgeeⁱ	ENSMUSG00000000805 Expressed in 226 organ(s), highest expression level in left colon
CleanExⁱ	MM_CAR4
ExpressionAtlasⁱ	Q64444 baseline and differential
Genevisibleⁱ	Q64444 MM

Family and domain databases

Gene3Dⁱ	3.10.200.10, 1 hit
InterProⁱ	View protein in InterPro IPR001148 CA_dom IPR036398 CA_dom_sf IPR023561 Carbonic_anhydrase_a-class IPR018338 Carbonic_anhydrase_a-class_CS IPR018343 Carbonic_anhydrase_CA4
PANTHERⁱ	PTHR18952 PTHR18952, 1 hit PTHR18952:SF95 PTHR18952:SF95, 1 hit
Pfamⁱ	View protein in Pfam PF00194 Carb_anhydrase, 1 hit
SMARTⁱ	View protein in SMART SM01057 Carb_anhydrase, 1 hit
SUPFAMⁱ	SSF51069 SSF51069, 1 hit
PROSITEⁱ	View protein in PROSITE PS00162 ALPHA_CA_1, 1 hit PS51144 ALPHA_CA_2, 1 hit
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	CAH4_MOUSE
Accessionⁱ	Q64444Primary (citable) accession number: Q64444
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
	Last sequence update:	November 1, 1997
	Last modified:	November 7, 2018
	This is version 152 of the entry and version 1 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Chordata Protein Annotation Program

Miscellaneousⁱ

Keywords - Technical termⁱ

3D-structure, Complete proteome, Reference proteome

Documents

SIMILARITY comments
Index of protein domains and families
MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
PDB cross-references
Index of Protein Data Bank (PDB) cross-references

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UniProtKB - Q64444 (CAH4_MOUSE)

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Carbonic anhydrase 4

Ca4

Select a section on the left to see content.

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Sites

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

Enzyme and pathway databases

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

Organism-specific databases

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Plasma membrane

Endoplasmic reticulum

Extracellular region or secreted

Golgi apparatus

Plasma Membrane

Other locations

Keywords - Cellular componenti

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Amino acid modifications

Keywords - PTMi

Proteomic databases

PTM databases

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

Gene expression databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

Protein-protein interaction databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

Secondary structure

3D structure databases

Miscellaneous databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

Region

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Keywords - Domaini

Phylogenomic databases

Family and domain databases

Sequence databases

Genome annotation databases

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Miscellaneous databases

Gene expression databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

Documents

UniProt is an ELIXIR core data resource

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Functionⁱ

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

Keywords - Cellular componentⁱ

PTM / Processingⁱ

Keywords - PTMⁱ

Expressionⁱ

Interactionⁱ

Structureⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Keywords - Domainⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ

Keywords - Technical termⁱ