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Protein

Carbonic anhydrase 4

Gene

Ca4

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Reversible hydration of carbon dioxide. May stimulate the sodium/bicarbonate transporter activity of SLC4A4 (By similarity).By similarity

Catalytic activityi

H2CO3 = CO2 + H2O.

Cofactori

Zn2+1 Publication

Activity regulationi

Inhibited by acetazolamide.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei87Proton acceptorBy similarity1
Metal bindingi114Zinc; catalytic1 Publication1
Metal bindingi116Zinc; catalytic1 Publication1
Metal bindingi139Zinc; catalytic1 Publication1

GO - Molecular functioni

  • carbonate dehydratase activity Source: MGI
  • zinc ion binding Source: InterPro

GO - Biological processi

  • bicarbonate transport Source: MGI
  • carbon dioxide transport Source: MGI
  • regulation of pH Source: MGI

Keywordsi

Molecular functionLyase
LigandMetal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-MMU-1237044 Erythrocytes take up carbon dioxide and release oxygen
R-MMU-1247673 Erythrocytes take up oxygen and release carbon dioxide
R-MMU-1475029 Reversible hydration of carbon dioxide

Names & Taxonomyi

Protein namesi
Recommended name:
Carbonic anhydrase 4 (EC:4.2.1.1)
Alternative name(s):
Carbonate dehydratase IV
Carbonic anhydrase IV
Short name:
CA-IV
Gene namesi
Name:Ca4
Synonyms:Car4
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 11

Organism-specific databases

MGIiMGI:1096574 Car4

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 17Sequence analysisAdd BLAST17
ChainiPRO_000000422818 – 277Carbonic anhydrase 4Add BLAST260
PropeptideiPRO_0000004229278 – 305Removed in mature formSequence analysisAdd BLAST28

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi23 ↔ 351 Publication
Disulfide bondi45 ↔ 2221 Publication
Glycosylationi123N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi214N-linked (GlcNAc...) asparagineSequence analysis1
Lipidationi277GPI-anchor amidated serineBy similarity1

Keywords - PTMi

Disulfide bond, Glycoprotein, GPI-anchor, Lipoprotein

Proteomic databases

MaxQBiQ64444
PaxDbiQ64444
PeptideAtlasiQ64444
PRIDEiQ64444

PTM databases

PhosphoSitePlusiQ64444

Expressioni

Gene expression databases

BgeeiENSMUSG00000000805 Expressed in 226 organ(s), highest expression level in left colon
CleanExiMM_CAR4
ExpressionAtlasiQ64444 baseline and differential
GenevisibleiQ64444 MM

Interactioni

Subunit structurei

Interacts with SLC4A4.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
Slc4a4O883432EBI-6859308,EBI-771342

Protein-protein interaction databases

IntActiQ64444, 2 interactors
MINTiQ64444
STRINGi10090.ENSMUSP00000099483

Structurei

Secondary structure

1305
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliQ64444
SMRiQ64444
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ64444

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini20 – 278Alpha-carbonic anhydrasePROSITE-ProRule annotationAdd BLAST259

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni218 – 219Substrate bindingBy similarity2

Sequence similaritiesi

Belongs to the alpha-carbonic anhydrase family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG0382 Eukaryota
COG3338 LUCA
GeneTreeiENSGT00760000118915
HOGENOMiHOG000112637
HOVERGENiHBG002837
InParanoidiQ64444
KOiK18246
OMAiESHWCYE
OrthoDBiEOG091G0XFM
TreeFamiTF316425

Family and domain databases

Gene3Di3.10.200.10, 1 hit
InterProiView protein in InterPro
IPR001148 CA_dom
IPR036398 CA_dom_sf
IPR023561 Carbonic_anhydrase_a-class
IPR018338 Carbonic_anhydrase_a-class_CS
IPR018343 Carbonic_anhydrase_CA4
PANTHERiPTHR18952 PTHR18952, 1 hit
PTHR18952:SF95 PTHR18952:SF95, 1 hit
PfamiView protein in Pfam
PF00194 Carb_anhydrase, 1 hit
SMARTiView protein in SMART
SM01057 Carb_anhydrase, 1 hit
SUPFAMiSSF51069 SSF51069, 1 hit
PROSITEiView protein in PROSITE
PS00162 ALPHA_CA_1, 1 hit
PS51144 ALPHA_CA_2, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry has 1 described isoform and 2 potential isoforms that are computationally mapped.Show allAlign All

Q64444-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MQLLLALLAL AYVAPSTEDS GWCYEIQTKD PRSSCLGPEK WPGACKENQQ
60 70 80 90 100
SPINIVTART KVNPRLTPFI LVGYDQKQQW PIKNNQHTVE MTLGGGACII
110 120 130 140 150
GGDLPARYEA VQLHLHWSNG NDNGSEHSID GRHFAMEMHI VHKKLTSSKE
160 170 180 190 200
DSKDKFAVLA FMIEVGDKVN KGFQPLVEAL PSISKPHSTS TVRESSLQDM
210 220 230 240 250
LPPSTKMYTY FRYNGSLTTP NCDETVIWTV YKQPIKIHKN QFLEFSKNLY
260 270 280 290 300
YDEDQKLNMK DNVRPLQPLG KRQVFKSHAP GQLLSLPLPT LLVPTLTCLV

ANFLQ
Length:305
Mass (Da):34,351
Last modified:November 1, 1997 - v1
Checksum:iEEE988FF52732884
GO

Computationally mapped potential isoform sequencesi

There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
F6ST32F6ST32_MOUSE
Carbonic anhydrase 4
Car4
164Annotation score:
D6RCZ3D6RCZ3_MOUSE
Carbonic anhydrase 4
Car4
38Annotation score:

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U37091 Genomic DNA Translation: AAC52569.1
BC012704 mRNA Translation: AAH12704.1
CCDSiCCDS25190.1
RefSeqiNP_031633.1, NM_007607.2
UniGeneiMm.1641

Genome annotation databases

EnsembliENSMUST00000103194; ENSMUSP00000099483; ENSMUSG00000000805
GeneIDi12351
KEGGimmu:12351
UCSCiuc007krg.2 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U37091 Genomic DNA Translation: AAC52569.1
BC012704 mRNA Translation: AAH12704.1
CCDSiCCDS25190.1
RefSeqiNP_031633.1, NM_007607.2
UniGeneiMm.1641

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2ZNCX-ray2.80A22-279[»]
3ZNCX-ray2.80A22-279[»]
ProteinModelPortaliQ64444
SMRiQ64444
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ64444, 2 interactors
MINTiQ64444
STRINGi10090.ENSMUSP00000099483

PTM databases

PhosphoSitePlusiQ64444

Proteomic databases

MaxQBiQ64444
PaxDbiQ64444
PeptideAtlasiQ64444
PRIDEiQ64444

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000103194; ENSMUSP00000099483; ENSMUSG00000000805
GeneIDi12351
KEGGimmu:12351
UCSCiuc007krg.2 mouse

Organism-specific databases

CTDi12351
MGIiMGI:1096574 Car4

Phylogenomic databases

eggNOGiKOG0382 Eukaryota
COG3338 LUCA
GeneTreeiENSGT00760000118915
HOGENOMiHOG000112637
HOVERGENiHBG002837
InParanoidiQ64444
KOiK18246
OMAiESHWCYE
OrthoDBiEOG091G0XFM
TreeFamiTF316425

Enzyme and pathway databases

ReactomeiR-MMU-1237044 Erythrocytes take up carbon dioxide and release oxygen
R-MMU-1247673 Erythrocytes take up oxygen and release carbon dioxide
R-MMU-1475029 Reversible hydration of carbon dioxide

Miscellaneous databases

EvolutionaryTraceiQ64444
PROiPR:Q64444
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000000805 Expressed in 226 organ(s), highest expression level in left colon
CleanExiMM_CAR4
ExpressionAtlasiQ64444 baseline and differential
GenevisibleiQ64444 MM

Family and domain databases

Gene3Di3.10.200.10, 1 hit
InterProiView protein in InterPro
IPR001148 CA_dom
IPR036398 CA_dom_sf
IPR023561 Carbonic_anhydrase_a-class
IPR018338 Carbonic_anhydrase_a-class_CS
IPR018343 Carbonic_anhydrase_CA4
PANTHERiPTHR18952 PTHR18952, 1 hit
PTHR18952:SF95 PTHR18952:SF95, 1 hit
PfamiView protein in Pfam
PF00194 Carb_anhydrase, 1 hit
SMARTiView protein in SMART
SM01057 Carb_anhydrase, 1 hit
SUPFAMiSSF51069 SSF51069, 1 hit
PROSITEiView protein in PROSITE
PS00162 ALPHA_CA_1, 1 hit
PS51144 ALPHA_CA_2, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiCAH4_MOUSE
AccessioniPrimary (citable) accession number: Q64444
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: November 7, 2018
This is version 152 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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