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Protein

10 kDa heat shock protein, mitochondrial

Gene

Hspe1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Co-chaperonin implicated in mitochondrial protein import and macromolecular assembly. Together with Hsp60, facilitates the correct folding of imported proteins. May also prevent misfolding and promote the refolding and proper assembly of unfolded polypeptides generated under stress conditions in the mitochondrial matrix. The functional units of these chaperonins consist of heptameric rings of the large subunit Hsp60, which function as a back-to-back double ring. In a cyclic reaction, Hsp60 ring complexes bind one unfolded substrate protein per ring, followed by the binding of ATP and association with 2 heptameric rings of the co-chaperonin Hsp10. This leads to sequestration of the substrate protein in the inner cavity of Hsp60 where, for a certain period of time, it can fold undisturbed by other cell components. Synchronous hydrolysis of ATP in all Hsp60 subunits results in the dissociation of the chaperonin rings and the release of ADP and the folded substrate protein.By similarity

GO - Molecular functioni

  • ATP binding Source: InterPro
  • chaperone binding Source: UniProtKB
  • metal ion binding Source: GO_Central
  • unfolded protein binding Source: GO_Central

GO - Biological processi

Keywordsi

Molecular functionChaperone
Biological processStress response

Names & Taxonomyi

Protein namesi
Recommended name:
10 kDa heat shock protein, mitochondrial
Short name:
Hsp10
Alternative name(s):
10 kDa chaperonin
Chaperonin 10
Short name:
CPN10
Gene namesi
Name:Hspe1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 1

Organism-specific databases

MGIiMGI:104680 Hspe1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00001749182 – 10210 kDa heat shock protein, mitochondrialAdd BLAST101

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineBy similarity1
Modified residuei8N6-acetyllysineCombined sources1
Modified residuei28N6-succinyllysineCombined sources1
Modified residuei40N6-acetyllysine; alternateCombined sources1
Modified residuei40N6-malonyllysine; alternateBy similarity1
Modified residuei40N6-succinyllysine; alternateCombined sources1
Modified residuei54N6-malonyllysine; alternateBy similarity1
Modified residuei54N6-succinyllysine; alternateCombined sources1
Modified residuei56N6-acetyllysine; alternateCombined sources1
Modified residuei56N6-malonyllysine; alternateBy similarity1
Modified residuei56N6-succinyllysine; alternateCombined sources1
Modified residuei57PhosphoserineCombined sources1
Modified residuei66N6-acetyllysine; alternateCombined sources1
Modified residuei66N6-succinyllysine; alternateCombined sources1
Modified residuei70N6-acetyllysine; alternateCombined sources1
Modified residuei70N6-succinyllysine; alternateCombined sources1
Modified residuei79PhosphothreonineBy similarity1
Modified residuei80N6-acetyllysine; alternateCombined sources1
Modified residuei80N6-succinyllysine; alternateCombined sources1
Modified residuei86N6-acetyllysine; alternateCombined sources1
Modified residuei86N6-succinyllysine; alternateCombined sources1
Modified residuei99N6-acetyllysineCombined sources1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ64433
MaxQBiQ64433
PaxDbiQ64433
PRIDEiQ64433
TopDownProteomicsiQ64433

2D gel databases

REPRODUCTION-2DPAGEiQ64433

PTM databases

iPTMnetiQ64433
PhosphoSitePlusiQ64433

Expressioni

Inductioni

By stress.

Gene expression databases

BgeeiENSMUSG00000073676 Expressed in 268 organ(s), highest expression level in epiblast (generic)
CleanExiMM_HSPE1
ExpressionAtlasiQ64433 baseline and differential
GenevisibleiQ64433 MM

Interactioni

Subunit structurei

Homoheptamer arranged in a ring structure. 2 heptameric Hsp10 rings interact with a Hsp60 tetradecamer in the structure of a back-to-back double heptameric ring to form the symmetrical football complex.By similarity

GO - Molecular functioni

Protein-protein interaction databases

BioGridi200459, 3 interactors
IntActiQ64433, 2 interactors
MINTiQ64433
STRINGi10090.ENSMUSP00000074724

Structurei

3D structure databases

ProteinModelPortaliQ64433
SMRiQ64433
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the GroES chaperonin family.Curated

Phylogenomic databases

eggNOGiKOG1641 Eukaryota
COG0234 LUCA
GeneTreeiENSGT00390000006350
HOGENOMiHOG000133897
HOVERGENiHBG000385
InParanoidiQ64433
KOiK04078
OMAiPGRIDDN
OrthoDBiEOG091G0ZIY
PhylomeDBiQ64433
TreeFamiTF313814

Family and domain databases

CDDicd00320 cpn10, 1 hit
Gene3Di2.30.33.40, 1 hit
HAMAPiMF_00580 CH10, 1 hit
InterProiView protein in InterPro
IPR020818 Chaperonin_GroES
IPR037124 Chaperonin_GroES_sf
IPR018369 Chaprnonin_Cpn10_CS
IPR011032 GroES-like_sf
PANTHERiPTHR10772 PTHR10772, 1 hit
PfamiView protein in Pfam
PF00166 Cpn10, 1 hit
PRINTSiPR00297 CHAPERONIN10
SMARTiView protein in SMART
SM00883 Cpn10, 1 hit
SUPFAMiSSF50129 SSF50129, 1 hit
PROSITEiView protein in PROSITE
PS00681 CHAPERONINS_CPN10, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q64433-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MAGQAFRKFL PLFDRVLVER SAAETVTKGG IMLPEKSQGK VLQATVVAVG
60 70 80 90 100
SGGKGKSGEI EPVSVKVGDK VLLPEYGGTK VVLDDKDYFL FRDSDILGKY

VD
Length:102
Mass (Da):10,963
Last modified:January 23, 2007 - v2
Checksum:i08BEB5566DBE8A3D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U09659 mRNA Translation: AAA62229.1
AF251024 Genomic DNA Translation: AAF67345.1
AK088121 mRNA Translation: BAC40159.1
BC024385 mRNA Translation: AAH24385.1
CCDSiCCDS35570.1
PIRiA55075
RefSeqiNP_032329.1, NM_008303.4
UniGeneiMm.215667

Genome annotation databases

EnsembliENSMUST00000075242; ENSMUSP00000074724; ENSMUSG00000073676
GeneIDi15528
KEGGimmu:15528
UCSCiuc007bac.2 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U09659 mRNA Translation: AAA62229.1
AF251024 Genomic DNA Translation: AAF67345.1
AK088121 mRNA Translation: BAC40159.1
BC024385 mRNA Translation: AAH24385.1
CCDSiCCDS35570.1
PIRiA55075
RefSeqiNP_032329.1, NM_008303.4
UniGeneiMm.215667

3D structure databases

ProteinModelPortaliQ64433
SMRiQ64433
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi200459, 3 interactors
IntActiQ64433, 2 interactors
MINTiQ64433
STRINGi10090.ENSMUSP00000074724

PTM databases

iPTMnetiQ64433
PhosphoSitePlusiQ64433

2D gel databases

REPRODUCTION-2DPAGEiQ64433

Proteomic databases

EPDiQ64433
MaxQBiQ64433
PaxDbiQ64433
PRIDEiQ64433
TopDownProteomicsiQ64433

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000075242; ENSMUSP00000074724; ENSMUSG00000073676
GeneIDi15528
KEGGimmu:15528
UCSCiuc007bac.2 mouse

Organism-specific databases

CTDi3336
MGIiMGI:104680 Hspe1

Phylogenomic databases

eggNOGiKOG1641 Eukaryota
COG0234 LUCA
GeneTreeiENSGT00390000006350
HOGENOMiHOG000133897
HOVERGENiHBG000385
InParanoidiQ64433
KOiK04078
OMAiPGRIDDN
OrthoDBiEOG091G0ZIY
PhylomeDBiQ64433
TreeFamiTF313814

Miscellaneous databases

PROiPR:Q64433
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000073676 Expressed in 268 organ(s), highest expression level in epiblast (generic)
CleanExiMM_HSPE1
ExpressionAtlasiQ64433 baseline and differential
GenevisibleiQ64433 MM

Family and domain databases

CDDicd00320 cpn10, 1 hit
Gene3Di2.30.33.40, 1 hit
HAMAPiMF_00580 CH10, 1 hit
InterProiView protein in InterPro
IPR020818 Chaperonin_GroES
IPR037124 Chaperonin_GroES_sf
IPR018369 Chaprnonin_Cpn10_CS
IPR011032 GroES-like_sf
PANTHERiPTHR10772 PTHR10772, 1 hit
PfamiView protein in Pfam
PF00166 Cpn10, 1 hit
PRINTSiPR00297 CHAPERONIN10
SMARTiView protein in SMART
SM00883 Cpn10, 1 hit
SUPFAMiSSF50129 SSF50129, 1 hit
PROSITEiView protein in PROSITE
PS00681 CHAPERONINS_CPN10, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiCH10_MOUSE
AccessioniPrimary (citable) accession number: Q64433
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: January 23, 2007
Last modified: November 7, 2018
This is version 148 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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