UniProtKB - Q64429 (CP1B1_MOUSE)
Cytochrome P450 1B1
Cyp1b1
Functioni
A cytochrome P450 monooxygenase involved in the metabolism of various endogenous substrates, including fatty acids, steroid hormones and vitamins (By similarity).
Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (NADPH--hemoprotein reductase) (By similarity).
Exhibits catalytic activity for the formation of hydroxyestrogens from 17beta-estradiol (E2), namely 2- and 4-hydroxy E2 (PubMed:23821647).
Metabolizes testosterone and progesterone to B or D ring hydroxylated metabolites (By similarity).
May act as a major enzyme for all-trans retinoic acid biosynthesis in extrahepatic tissues. Catalyzes two successive oxidative transformation of all-trans retinol to all-trans retinal and then to the active form all-trans retinoic acid (PubMed:15258110).
Catalyzes the epoxidation of double bonds of certain PUFA. Converts arachidonic acid toward epoxyeicosatrienoic acid (EpETrE) regioisomers, 8,9-, 11,12-, and 14,15- EpETrE, that function as lipid mediators in the vascular system (PubMed:15258110).
Additionally, displays dehydratase activity toward oxygenated eicosanoids hydroperoxyeicosatetraenoates (HpETEs). This activity is independent of cytochrome P450 reductase, NADPH, and O2 (By similarity).
Also involved in the oxidative metabolism of xenobiotics, particularly converting polycyclic aromatic hydrocarbons and heterocyclic aryl amines procarcinogens to DNA-damaging products (By similarity).
Plays an important role in retinal vascular development. Under ambient/hyperoxic O2 conditions, promotes angiogenesis and capillary morphogenesis of retinal endothelial cells and pericytes, likely by metabolizing the oxygenated products symptomatic of oxidative stress (PubMed:19005183, PubMed:20032512, PubMed:23568032).
Also, contributes to oxidative homeostasis and ultrastructural organization and function of trabecular meshwork tissue through modulation of POSTN expression (PubMed:23979599).
By similarity6 PublicationsCatalytic activityi
- an organic molecule + O2 + reduced [NADPH—hemoprotein reductase] = an alcohol + H+ + H2O + oxidized [NADPH—hemoprotein reductase]1 PublicationEC:1.14.14.11 PublicationThis reaction proceeds in the forward1 Publication direction.
- 17β-estradiol + O2 + reduced [NADPH—hemoprotein reductase] = 2-hydroxy-17β-estradiol + H+ + H2O + oxidized [NADPH—hemoprotein reductase]1 PublicationThis reaction proceeds in the forward1 Publication direction.
- 17β-estradiol + O2 + reduced [NADPH—hemoprotein reductase] = 4-hydroxy-17β-estradiol + H+ + H2O + oxidized [NADPH—hemoprotein reductase]1 PublicationThis reaction proceeds in the forward1 Publication direction.
- estrone + O2 + reduced [NADPH—hemoprotein reductase] = 2-hydroxyestrone + H+ + H2O + oxidized [NADPH—hemoprotein reductase]By similarityThis reaction proceeds in the forwardBy similarity direction.
- estrone + O2 + reduced [NADPH—hemoprotein reductase] = 4-hydroxyestrone + H+ + H2O + oxidized [NADPH—hemoprotein reductase]By similarityThis reaction proceeds in the forwardBy similarity direction.
- O2 + reduced [NADPH—hemoprotein reductase] + testosterone = 6β,17β-dihydroxyandrost-4-en-3-one + H+ + H2O + oxidized [NADPH—hemoprotein reductase]By similarityThis reaction proceeds in the forwardBy similarity direction.
- O2 + progesterone + reduced [NADPH—hemoprotein reductase] = 6β-hydroxyprogesterone + H+ + H2O + oxidized [NADPH—hemoprotein reductase]By similarityThis reaction proceeds in the forwardBy similarity direction.
- O2 + progesterone + reduced [NADPH—hemoprotein reductase] = 16α-hydroxyprogesterone + H+ + H2O + oxidized [NADPH—hemoprotein reductase]By similarityThis reaction proceeds in the forwardBy similarity direction.
- all-trans-retinol + O2 + reduced [NADPH—hemoprotein reductase] = all-trans-retinal + H+ + 2 H2O + oxidized [NADPH—hemoprotein reductase]1 PublicationThis reaction proceeds in the forward1 Publication direction.
- all-trans-retinal + O2 + reduced [NADPH—hemoprotein reductase] = all-trans-retinoate + 2 H+ + H2O + oxidized [NADPH—hemoprotein reductase]1 PublicationThis reaction proceeds in the forward1 Publication direction.
- (5Z,8Z,11Z,14Z)-eicosatetraenoate + O2 + reduced [NADPH—hemoprotein reductase] = (8R,9S)-epoxy-(5Z,11Z,14Z)-eicosatrienoate + H+ + H2O + oxidized [NADPH—hemoprotein reductase]By similarityThis reaction proceeds in the forwardBy similarity direction.
- (5Z,8Z,11Z,14Z)-eicosatetraenoate + O2 + reduced [NADPH—hemoprotein reductase] = (11R,12S)-epoxy-(5Z,8Z,14Z)-eicosatrienoate + H+ + H2O + oxidized [NADPH—hemoprotein reductase]By similarityThis reaction proceeds in the forwardBy similarity direction.
- (5Z,8Z,11Z,14Z)-eicosatetraenoate + O2 + reduced [NADPH—hemoprotein reductase] = (11S,12R)-epoxy-(5Z,8Z,14Z)-eicosatrienoate + H+ + H2O + oxidized [NADPH—hemoprotein reductase]By similarityThis reaction proceeds in the forwardBy similarity direction.
- (5Z,8Z,11Z,14Z)-eicosatetraenoate + O2 + reduced [NADPH—hemoprotein reductase] = (14R,15S)-epoxy-(5Z,8Z,11Z)-eicosatrienoate + H+ + H2O + oxidized [NADPH—hemoprotein reductase]By similarityThis reaction proceeds in the forwardBy similarity direction.
- (5S)-hydroperoxy-(6E,8Z,11Z,14Z)-eicosatetraenoate = 5-oxo-(6E,8Z,11Z,14Z)-eicosatetraenoate + H2OBy similarityThis reaction proceeds in the forwardBy similarity direction.
- (12S)-hydroperoxy-(5Z,8Z,10E,14Z)-eicosatetraenoate = 12-oxo-(5Z,8Z,10E,14Z)-eicosatetraenoate + H2OBy similarityEC:4.2.1.152By similarityThis reaction proceeds in the forwardBy similarity direction.
- This reaction proceeds in the forwardBy similarity direction.
- (15S)-hydroperoxy-(5Z,8Z,11Z,13E)-eicosatetraenoate = 15-oxo-(5Z,8Z,11Z,13E)-eicosatetraenoate + H2OBy similarityThis reaction proceeds in the forwardBy similarity direction.
Cofactori
Activity regulationi
Kineticsi
- KM=392.2 µM for all-trans-retinol1 Publication
- KM=153.9 µM for all-trans-retinal1 Publication
- KM=138.9 µM for 7,12-dimethyltetraphene1 Publication
- KM=500.0 µM for arachidonic acid1 Publication
: Steroid hormone biosynthesis Pathwayi
This protein is involved in Steroid hormone biosynthesis.By similarityView all proteins of this organism that are known to be involved in Steroid hormone biosynthesis.
Pathwayi: retinol metabolism
This protein is involved in the pathway retinol metabolism, which is part of Cofactor metabolism.By similarityView all proteins of this organism that are known to be involved in the pathway retinol metabolism and in Cofactor metabolism.
Pathwayi: arachidonate metabolism
This protein is involved in the pathway arachidonate metabolism, which is part of Lipid metabolism.By similarityView all proteins of this organism that are known to be involved in the pathway arachidonate metabolism and in Lipid metabolism.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 470 | Iron (heme axial ligand)By similarity | 1 |
GO - Molecular functioni
- aromatase activity Source: UniProtKB-EC
- estrogen 16-alpha-hydroxylase activity Source: UniProtKB
- heme binding Source: UniProtKB
- hydroperoxy icosatetraenoate dehydratase activity Source: UniProtKB-EC
- iron ion binding Source: InterPro
- monooxygenase activity Source: UniProtKB
- oxidoreductase activity Source: MGI
- oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen Source: MGI
GO - Biological processi
- adrenal gland development Source: Ensembl
- angiogenesis Source: MGI
- arachidonic acid metabolic process Source: UniProtKB
- benzene-containing compound metabolic process Source: MGI
- blood vessel morphogenesis Source: UniProtKB
- cell adhesion Source: UniProtKB
- cellular aromatic compound metabolic process Source: MGI
- cellular response to cAMP Source: Ensembl
- cellular response to cortisol stimulus Source: Ensembl
- cellular response to hydrogen peroxide Source: UniProtKB
- cellular response to luteinizing hormone stimulus Source: Ensembl
- cellular response to organic cyclic compound Source: MGI
- cellular response to progesterone stimulus Source: Ensembl
- cellular response to tumor necrosis factor Source: Ensembl
- collagen fibril organization Source: UniProtKB
- DNA modification Source: MGI
- endothelial cell-cell adhesion Source: MGI
- endothelial cell migration Source: UniProtKB
- estrogen metabolic process Source: UniProtKB
- estrous cycle Source: Ensembl
- ganglion development Source: Ensembl
- intrinsic apoptotic signaling pathway in response to oxidative stress Source: UniProtKB
- male gonad development Source: Ensembl
- membrane lipid catabolic process Source: UniProtKB
- negative regulation of cell adhesion mediated by integrin Source: UniProtKB
- negative regulation of cell migration Source: UniProtKB
- negative regulation of cell population proliferation Source: UniProtKB
- negative regulation of NF-kappaB transcription factor activity Source: UniProtKB
- nitric oxide biosynthetic process Source: UniProtKB
- positive regulation of angiogenesis Source: UniProtKB
- positive regulation of apoptotic process Source: UniProtKB
- positive regulation of DNA biosynthetic process Source: MGI
- positive regulation of reactive oxygen species metabolic process Source: MGI
- positive regulation of receptor signaling pathway via JAK-STAT Source: UniProtKB
- positive regulation of smooth muscle cell migration Source: MGI
- positive regulation of translation Source: MGI
- positive regulation of vascular endothelial growth factor production Source: UniProtKB
- regulation of reactive oxygen species metabolic process Source: UniProtKB
- response to arsenic-containing substance Source: Ensembl
- response to dexamethasone Source: Ensembl
- response to estradiol Source: Ensembl
- response to follicle-stimulating hormone Source: Ensembl
- response to indole-3-methanol Source: Ensembl
- response to nutrient Source: Ensembl
- response to toxic substance Source: MGI
- retinal blood vessel morphogenesis Source: UniProtKB
- retinal metabolic process Source: UniProtKB
- retina vasculature development in camera-type eye Source: MGI
- retinol metabolic process Source: UniProtKB
- steroid metabolic process Source: UniProtKB
- toxin metabolic process Source: MGI
- trabecular meshwork development Source: UniProtKB
- xenobiotic metabolic process Source: UniProtKB
Keywordsi
Molecular function | Lyase, Monooxygenase, Oxidoreductase |
Biological process | Fatty acid metabolism, Lipid metabolism, Steroid metabolism |
Ligand | Heme, Iron, Metal-binding |
Enzyme and pathway databases
Reactomei | R-MMU-211976, Endogenous sterols R-MMU-2142670, Synthesis of epoxy (EET) and dihydroxyeicosatrienoic acids (DHET) R-MMU-2142816, Synthesis of (16-20)-hydroxyeicosatetraenoic acids (HETE) |
SABIO-RKi | Q64429 |
UniPathwayi | UPA00383 UPA00912 |
Names & Taxonomyi
Protein namesi | |
Gene namesi | |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:88590, Cyp1b1 |
VEuPathDBi | HostDB:ENSMUSG00000024087 |
Subcellular locationi
Mitochondrion
- Mitochondrion 1 Publication
Endoplasmic reticulum
- Endoplasmic reticulum membrane 1 Publication; Peripheral membrane protein 1 Publication
- Microsome membrane 1 Publication; Peripheral membrane protein 1 Publication
Note: Located primarily in endoplasmic reticulum. Upon treatment with 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD), CYP1B1 is also targeted to mitochondria.1 Publication
Endoplasmic reticulum
- endoplasmic reticulum membrane Source: UniProtKB-SubCell
Mitochondrion
- mitochondrion Source: UniProtKB
Nucleus
- nucleus Source: MGI
Other locations
- intracellular membrane-bounded organelle Source: MGI
Keywords - Cellular componenti
Endoplasmic reticulum, Membrane, Microsome, MitochondrionPathology & Biotechi
Disruption phenotypei
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000051661 | 1 – 543 | Cytochrome P450 1B1Add BLAST | 543 |
Proteomic databases
jPOSTi | Q64429 |
MaxQBi | Q64429 |
PaxDbi | Q64429 |
PRIDEi | Q64429 |
ProteomicsDBi | 284107 |
PTM databases
iPTMneti | Q64429 |
PhosphoSitePlusi | Q64429 |
Expressioni
Tissue specificityi
Inductioni
Gene expression databases
Bgeei | ENSMUSG00000024087, Expressed in stroma of bone marrow and 234 other tissues |
ExpressionAtlasi | Q64429, baseline and differential |
Genevisiblei | Q64429, MM |
Interactioni
Protein-protein interaction databases
BioGRIDi | 199003, 3 interactors |
STRINGi | 10090.ENSMUSP00000024894 |
Miscellaneous databases
RNActi | Q64429, protein |
Family & Domainsi
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG0156, Eukaryota |
GeneTreei | ENSGT00950000183037 |
HOGENOMi | CLU_001570_22_0_1 |
InParanoidi | Q64429 |
OMAi | HAGQWLL |
OrthoDBi | 702827at2759 |
PhylomeDBi | Q64429 |
TreeFami | TF105095 |
Family and domain databases
Gene3Di | 1.10.630.10, 1 hit |
InterProi | View protein in InterPro IPR032971, CYP1B1 IPR001128, Cyt_P450 IPR017972, Cyt_P450_CS IPR002401, Cyt_P450_E_grp-I IPR036396, Cyt_P450_sf |
PANTHERi | PTHR24299:SF11, PTHR24299:SF11, 1 hit |
Pfami | View protein in Pfam PF00067, p450, 1 hit |
PRINTSi | PR00463, EP450I PR00385, P450 |
SUPFAMi | SSF48264, SSF48264, 1 hit |
PROSITEi | View protein in PROSITE PS00086, CYTOCHROME_P450, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 2 potential isoforms that are computationally mapped.Show allAlign All
10 20 30 40 50
MATSLSADSP QQLSSLSTQQ TTLLLLFSVL AAVHLGQWLL RQWQRKPWSS
60 70 80 90 100
PPGPFPWPLI GNAAAVGQAS HLYFARLARR YGDVFQIRLG SCPVVVLNGE
110 120 130 140 150
SAIHQALVQQ GSIFADRPPF ASFRVVSGGR SLAFGHYSEH WKTQRRSAYS
160 170 180 190 200
TMRAFSTRHP RSRGLLEGHA LAEARELVAV LVRRCAGGAF LDPTQPVIVA
210 220 230 240 250
VANVMSAVCF GCRYNHDDAE FLELLSHNEE FGRTVGAGSL VDVLPWLQLF
260 270 280 290 300
PNPVRTTFRK FEQLNRNFSN FVLDKFLRHR ESLVPGAAPR DMTDAFILSA
310 320 330 340 350
EKKASGAPGD DSSGLDLEDV PATITDIFGA SQDTLSTALL WLLILFTRYP
360 370 380 390 400
DVQARVQAEL DQVVGRDRLP CMSDQPNLPY VMAFLYESMR FSSFLPVTIP
410 420 430 440 450
HATTANTFVL GYYIPKNTVV FVNQWSVNHD PAKWPNPEDF DPARFLDKDG
460 470 480 490 500
FINKALASSV MIFSVGKRRC IGEELSKMLL FLFISILAHQ CNFKANQNES
510 520 530 540
SNMSFSYGLT IKPKSFRIHV SLRESMELLD NAVKKLQTEE GCK
Computationally mapped potential isoform sequencesi
There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketA0A3Q4L2V1 | A0A3Q4L2V1_MOUSE | Cytochrome P450 1B1 | Cyp1b1 | 289 | Annotation score: | ||
Q3UTK2 | Q3UTK2_MOUSE | Cytochrome P450 1B1 | Cyp1b1 | 487 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 195 | Q → P in CAA55205 (PubMed:8195121).Curated | 1 | |
Sequence conflicti | 307 | A → D in CAA55205 (PubMed:8195121).Curated | 1 | |
Sequence conflicti | 328 | F → G in CAA55205 (PubMed:8195121).Curated | 1 | |
Sequence conflicti | 457 | A → T in CAA55205 (PubMed:8195121).Curated | 1 | |
Sequence conflicti | 516 | F → V in CAA55205 (PubMed:8195121).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U03283 mRNA Translation: AAC52141.1 X78445 mRNA Translation: CAA55205.1 AK137461 mRNA Translation: BAE23362.1 CH466537 Genomic DNA Translation: EDL38490.1 U02479 mRNA Translation: AAC52131.1 |
CCDSi | CCDS28986.1 |
PIRi | A53790 |
RefSeqi | NP_034124.1, NM_009994.1 |
Genome annotation databases
Ensembli | ENSMUST00000024894; ENSMUSP00000024894; ENSMUSG00000024087 |
GeneIDi | 13078 |
KEGGi | mmu:13078 |
UCSCi | uc008dqc.1, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U03283 mRNA Translation: AAC52141.1 X78445 mRNA Translation: CAA55205.1 AK137461 mRNA Translation: BAE23362.1 CH466537 Genomic DNA Translation: EDL38490.1 U02479 mRNA Translation: AAC52131.1 |
CCDSi | CCDS28986.1 |
PIRi | A53790 |
RefSeqi | NP_034124.1, NM_009994.1 |
3D structure databases
SMRi | Q64429 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 199003, 3 interactors |
STRINGi | 10090.ENSMUSP00000024894 |
PTM databases
iPTMneti | Q64429 |
PhosphoSitePlusi | Q64429 |
Proteomic databases
jPOSTi | Q64429 |
MaxQBi | Q64429 |
PaxDbi | Q64429 |
PRIDEi | Q64429 |
ProteomicsDBi | 284107 |
Protocols and materials databases
Antibodypediai | 29477, 449 antibodies from 36 providers |
DNASUi | 13078 |
Genome annotation databases
Ensembli | ENSMUST00000024894; ENSMUSP00000024894; ENSMUSG00000024087 |
GeneIDi | 13078 |
KEGGi | mmu:13078 |
UCSCi | uc008dqc.1, mouse |
Organism-specific databases
CTDi | 1545 |
MGIi | MGI:88590, Cyp1b1 |
VEuPathDBi | HostDB:ENSMUSG00000024087 |
Phylogenomic databases
eggNOGi | KOG0156, Eukaryota |
GeneTreei | ENSGT00950000183037 |
HOGENOMi | CLU_001570_22_0_1 |
InParanoidi | Q64429 |
OMAi | HAGQWLL |
OrthoDBi | 702827at2759 |
PhylomeDBi | Q64429 |
TreeFami | TF105095 |
Enzyme and pathway databases
UniPathwayi | UPA00383 UPA00912 |
Reactomei | R-MMU-211976, Endogenous sterols R-MMU-2142670, Synthesis of epoxy (EET) and dihydroxyeicosatrienoic acids (DHET) R-MMU-2142816, Synthesis of (16-20)-hydroxyeicosatetraenoic acids (HETE) |
SABIO-RKi | Q64429 |
Miscellaneous databases
BioGRID-ORCSi | 13078, 2 hits in 65 CRISPR screens |
ChiTaRSi | Cyp1b1, mouse |
PROi | PR:Q64429 |
RNActi | Q64429, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000024087, Expressed in stroma of bone marrow and 234 other tissues |
ExpressionAtlasi | Q64429, baseline and differential |
Genevisiblei | Q64429, MM |
Family and domain databases
Gene3Di | 1.10.630.10, 1 hit |
InterProi | View protein in InterPro IPR032971, CYP1B1 IPR001128, Cyt_P450 IPR017972, Cyt_P450_CS IPR002401, Cyt_P450_E_grp-I IPR036396, Cyt_P450_sf |
PANTHERi | PTHR24299:SF11, PTHR24299:SF11, 1 hit |
Pfami | View protein in Pfam PF00067, p450, 1 hit |
PRINTSi | PR00463, EP450I PR00385, P450 |
SUPFAMi | SSF48264, SSF48264, 1 hit |
PROSITEi | View protein in PROSITE PS00086, CYTOCHROME_P450, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | CP1B1_MOUSE | |
Accessioni | Q64429Primary (citable) accession number: Q64429 Secondary accession number(s): Q3UVA8, Q60593, Q64461 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | December 15, 1998 |
Last sequence update: | July 27, 2011 | |
Last modified: | February 23, 2022 | |
This is version 167 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families