UniProtKB - Q64408 (C11B1_CAVPO)
Cytochrome P450 11B1, mitochondrial
CYP11B1
Functioni
A cytochrome P450 monooxygenase involved in the biosynthesis of adrenal corticoids. Catalyzes the hydroxylation of carbon hydrogen bond at 11-beta position of 11-deoxycortisol and 11-deoxycorticosterone/21-hydroxyprogesterone yielding cortisol or corticosterone, respectively. Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate and reducing the second into a water molecule. Two electrons are provided by NADPH via a two-protein mitochondrial transfer system comprising flavoprotein FDXR (adrenodoxin/ferredoxin reductase) and nonheme iron-sulfur protein FDX1 or FDX2 (adrenodoxin/ferredoxin).
By similarityCatalytic activityi
- a steroid + 2 H+ + O2 + 2 reduced [adrenodoxin] = an 11β-hydroxysteroid + H2O + 2 oxidized [adrenodoxin]By similarityEC:1.14.15.4By similarityThis reaction proceeds in the forwardBy similarity direction.
- 11-deoxycortisol + 2 H+ + O2 + 2 reduced [adrenodoxin] = cortisol + H2O + 2 oxidized [adrenodoxin]By similarityThis reaction proceeds in the forwardBy similarity direction.
- 21-hydroxyprogesterone + 2 H+ + O2 + 2 reduced [adrenodoxin] = corticosterone + H2O + 2 oxidized [adrenodoxin]By similarityThis reaction proceeds in the forwardBy similarity direction.
Cofactori
: glucocorticoid biosynthesis Pathwayi
This protein is involved in the pathway glucocorticoid biosynthesis, which is part of Steroid biosynthesis.By similarityView all proteins of this organism that are known to be involved in the pathway glucocorticoid biosynthesis and in Steroid biosynthesis.
Pathwayi: Steroid hormone biosynthesis
This protein is involved in Steroid hormone biosynthesis.By similarityView all proteins of this organism that are known to be involved in Steroid hormone biosynthesis.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 447 | Iron (heme axial ligand)By similarity | 1 |
GO - Molecular functioni
- heme binding Source: InterPro
- iron ion binding Source: InterPro
- steroid 11-beta-monooxygenase activity Source: UniProtKB-EC
GO - Biological processi
- glucocorticoid biosynthetic process Source: UniProtKB-UniPathway
Keywordsi
Molecular function | Monooxygenase, Oxidoreductase |
Biological process | Lipid metabolism, Steroid metabolism, Steroidogenesis |
Ligand | Heme, Iron, Metal-binding |
Enzyme and pathway databases
UniPathwayi | UPA00788 |
Names & Taxonomyi
Protein namesi | Recommended name: Cytochrome P450 11B1, mitochondrialAlternative name(s): Aldosterone synthase CYPXIB1 Cytochrome P450C11 Steroid 11-beta-hydroxylase, CYP11B1By similarity (EC:1.14.15.4By similarity) |
Gene namesi | Name:CYP11B1 |
Organismi | Cavia porcellus (Guinea pig) |
Taxonomic identifieri | 10141 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Hystricomorpha › Caviidae › Cavia |
Proteomesi |
|
Subcellular locationi
Mitochondrion
- Mitochondrion inner membrane By similarity; Peripheral membrane protein By similarity
Mitochondrion
- mitochondrial inner membrane Source: UniProtKB-SubCell
Keywords - Cellular componenti
Membrane, Mitochondrion, Mitochondrion inner membranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transit peptidei | 1 – 24 | MitochondrionAdd BLAST | 24 | |
ChainiPRO_0000003595 | 25 – 500 | Cytochrome P450 11B1, mitochondrialAdd BLAST | 476 |
Proteomic databases
PRIDEi | Q64408 |
Family & Domainsi
Sequence similaritiesi
Keywords - Domaini
Transit peptidePhylogenomic databases
eggNOGi | KOG0159, Eukaryota |
HOGENOMi | CLU_001570_28_4_1 |
InParanoidi | Q64408 |
OMAi | QESMATE |
Family and domain databases
Gene3Di | 1.10.630.10, 1 hit |
InterProi | View protein in InterPro IPR001128, Cyt_P450 IPR017972, Cyt_P450_CS IPR002401, Cyt_P450_E_grp-I IPR036396, Cyt_P450_sf |
Pfami | View protein in Pfam PF00067, p450, 1 hit |
PRINTSi | PR00463, EP450I PR00385, P450 |
SUPFAMi | SSF48264, SSF48264, 1 hit |
PROSITEi | View protein in PROSITE PS00086, CYTOCHROME_P450, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
10 20 30 40 50
MAFRLKSDVR LAGSWLCLRG ARALGTRAVT ASKASVLPFE VIPQHQGNKR
60 70 80 90 100
QRVLQFWKEQ NHDDLHLEMH QTFQELGPIF RCDVGSTRIV AVMLPEDCAR
110 120 130 140 150
LHQAESPYPH RMHLEPWMAY REHRRQNLGV FLLNGPEWLS NRRWLNPNVL
160 170 180 190 200
SPKAVQNLLP MVDTVARDFS EALKQKVLQS AQGSLTMDMQ PDIHKYTVEV
210 220 230 240 250
SNFALFGERL GLFGCNPSSQ SLKFIHALEA VFKTTTQLMF LPRSLSRWMR
260 270 280 290 300
SQAWKEHFEA WDYISEYAEN RIQKKYEELA RGCSQYNSIV ANLMLQGNLP
310 320 330 340 350
LRAMKANIMD LVAGSVDTTA LPLMMTLFEL ARNPTVQQAL RQESMATEPN
360 370 380 390 400
IYENPQRLRM ELPLLWAAIK ETLRMYPVGL FLERFLTSPL VLQNYHIPAG
410 420 430 440 450
TLVHLNLYSM GRNPEVFLSP EHYNPQRWLE NKETYKHLAF GFGVRQCIGR
460 470 480 490 500
RLAEVEMLLF LHHVLKSFCV ETAFQEDVKF AYRFVMMPTS APLLTFRPVS
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Z69785 mRNA Translation: CAA93633.1 |
PIRi | JC4709 |
RefSeqi | NP_001166410.1, NM_001172939.1 |
Genome annotation databases
GeneIDi | 111755182 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Z69785 mRNA Translation: CAA93633.1 |
PIRi | JC4709 |
RefSeqi | NP_001166410.1, NM_001172939.1 |
3D structure databases
SMRi | Q64408 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 10141.ENSCPOP00000012206 |
Proteomic databases
PRIDEi | Q64408 |
Genome annotation databases
GeneIDi | 111755182 |
Organism-specific databases
CTDi | 1584 |
Phylogenomic databases
eggNOGi | KOG0159, Eukaryota |
HOGENOMi | CLU_001570_28_4_1 |
InParanoidi | Q64408 |
OMAi | QESMATE |
Enzyme and pathway databases
UniPathwayi | UPA00788 |
Family and domain databases
Gene3Di | 1.10.630.10, 1 hit |
InterProi | View protein in InterPro IPR001128, Cyt_P450 IPR017972, Cyt_P450_CS IPR002401, Cyt_P450_E_grp-I IPR036396, Cyt_P450_sf |
Pfami | View protein in Pfam PF00067, p450, 1 hit |
PRINTSi | PR00463, EP450I PR00385, P450 |
SUPFAMi | SSF48264, SSF48264, 1 hit |
PROSITEi | View protein in PROSITE PS00086, CYTOCHROME_P450, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | C11B1_CAVPO | |
Accessioni | Q64408Primary (citable) accession number: Q64408 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 15, 1998 |
Last sequence update: | November 1, 1996 | |
Last modified: | September 29, 2021 | |
This is version 110 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families