UniProtKB - Q643R3 (LPCT4_HUMAN)
Protein
Lysophospholipid acyltransferase LPCAT4
Gene
LPCAT4
Organism
Homo sapiens (Human)
Status
Functioni
Displays acyl-CoA-dependent lysophospholipid acyltransferase activity with a subset of lysophospholipids as substrates; converts lysophosphatidylethanolamine to phosphatidylethanolamine, lysophosphatidylcholine to phosphatidycholine, 1-alkenyl-lysophatidylethanolamine to 1-alkenyl-phosphatidylethanolamine, lysophosphatidylglycerol and alkyl-lysophosphatidylcholine to phosphatidylglycerol and alkyl-phosphatidylcholine, respectively. In contrast, has no lysophosphatidylinositol, glycerol-3-phosphate, diacylglycerol or lysophosphatidic acid acyltransferase activity. Prefers long chain acyl-CoAs (C16, C18) as acyl donors.1 Publication
Catalytic activityi
- a 1-acyl-sn-glycero-3-phosphoethanolamine + an acyl-CoA = a 1,2-diacyl-sn-glycero-3-phosphoethanolamine + CoA1 PublicationEC:2.3.1.n71 Publication
- 1-O-(1Z-alkenyl)-sn-glycero-3-phosphoethanolamine + an acyl-CoA = 1-O-(1Z-alkenyl)-2-acyl-sn-glycero-3-phosphoethanolamine + CoA1 PublicationEC:2.3.1.1211 Publication
- a 1-acyl-sn-glycero-3-phosphocholine + an acyl-CoA = a 1,2-diacyl-sn-glycero-3-phosphocholine + CoA1 PublicationEC:2.3.1.231 Publication
- 1-O-alkyl-sn-glycero-3-phosphocholine + acetyl-CoA = a 1-O-alkyl-2-acetyl-sn-glycero-3-phosphocholine + CoA1 PublicationEC:2.3.1.671 Publication
- a 1-acyl-sn-glycero-3-phospho-L-serine + an acyl-CoA = a 1,2-diacyl-sn-glycero-3-phospho-L-serine + CoA1 PublicationEC:2.3.1.n61 Publication
- a 1-acyl-sn-glycero-3-phosphoethanolamine + octanoyl-CoA = 1-acyl-2-octanoyl-sn-glycero-3-phosphoethanolamine + CoA1 PublicationThis reaction proceeds in the forward1 Publication direction.
- a 1-acyl-sn-glycero-3-phosphoethanolamine + hexadecanoyl-CoA = 1-acyl-2-hexadecanoyl-sn-glycero-3-phosphoethanolamine + CoA1 PublicationThis reaction proceeds in the forward1 Publication direction.
- a 1-acyl-sn-glycero-3-phosphoethanolamine + octadecanoyl-CoA = 1-acyl-2-octadecanoyl-sn-glycero-3-phosphoethanolamine + CoA1 PublicationThis reaction proceeds in the forward1 Publication direction.
- (9Z)-octadecenoyl-CoA + a 1-acyl-sn-glycero-3-phosphoethanolamine = 1-acyl-2-(9Z)-octadecenoyl-sn-glycero-3-phosphoethanolamine + CoA1 PublicationThis reaction proceeds in the forward1 Publication direction.
- (5Z,8Z,11Z,14Z)-eicosatetraenoyl-CoA + a 1-acyl-sn-glycero-3-phosphoethanolamine = 1-acyl-2-(5Z,8Z,11Z,14Z)-eicosatetraenoyl-sn-glycero-3-phosphoethanolamine + CoA1 PublicationThis reaction proceeds in the forward1 Publication direction.
- 1-O-(1Z-alkenyl)-sn-glycero-3-phosphoethanolamine + octanoyl-CoA = 1-O-(1Z)-alkenyl-2-octanoyl-sn-glycero-3-phosphoethanolamine + CoA1 PublicationThis reaction proceeds in the forward1 Publication direction.
- 1-O-(1Z-alkenyl)-sn-glycero-3-phosphoethanolamine + hexadecanoyl-CoA = 1-O-(1Z)-alkenyl-2-hexadecanoyl-sn-glycero-3-phosphoethanolamine + CoA1 PublicationThis reaction proceeds in the forward1 Publication direction.
- 1-O-(1Z-alkenyl)-sn-glycero-3-phosphoethanolamine + octadecanoyl-CoA = 1-O-(1Z)-alkenyl-2-octadecanoyl-sn-glycero-3-phosphoethanolamine + CoA1 PublicationThis reaction proceeds in the forward1 Publication direction.
- (9Z)-octadecenoyl-CoA + 1-O-(1Z-alkenyl)-sn-glycero-3-phosphoethanolamine = 1-O-(1Z)-alkenyl-2-(9Z)-octadecenoyl-sn-glycero-3-phosphoethanolamine + CoA1 PublicationThis reaction proceeds in the forward1 Publication direction.
- (5Z,8Z,11Z,14Z)-eicosatetraenoyl-CoA + 1-O-(1Z-alkenyl)-sn-glycero-3-phosphoethanolamine = 1-O-(1Z)-alkenyl-2-(5Z,8Z,11Z,14Z)-eicosatetraenoyl-sn-glycero-3-phosphoethanolamine + CoA1 PublicationThis reaction proceeds in the forward1 Publication direction.
- a 1-acyl-sn-glycero-3-phosphocholine + hexadecanoyl-CoA = 1-acyl-2-hexadecanoyl-sn-glycero-3-phosphocholine + CoABy similarityThis reaction proceeds in the forwardBy similarity direction.
- (9Z)-octadecenoyl-CoA + a 1-acyl-sn-glycero-3-phosphocholine = 1-acyl-2-(9Z)-octadecenoyl-sn-glycero-3-phosphocholine + CoA1 PublicationThis reaction proceeds in the forward1 Publication direction.
- (9Z)-octadecenoyl-CoA + 1-O-hexadecyl-sn-glycero-3-phosphocholine = 1-O-hexadecyl-2-(9Z)-octadecenoyl-sn-glycero-3-phosphocholine + CoA1 PublicationThis reaction proceeds in the forward1 Publication direction.
- (5Z,8Z,11Z,14Z)-eicosatetraenoyl-CoA + 1-O-hexadecyl-sn-glycero-3-phosphocholine = 1-O-hexadecyl-2-(5Z,8Z,11Z,14Z)-eicosatetraenoyl-sn-glycero-3-phosphocholine + CoA1 PublicationThis reaction proceeds in the forward1 Publication direction.
- (9Z)-octadecenoyl-CoA + 1-hexadecanoyl-sn-glycero-3-phospho-L-serine = 1-hexadecanoyl-2-(9Z-octadecenoyl)-sn-glycero-3-phospho-L-serine + CoA1 PublicationThis reaction proceeds in the forward1 Publication direction.
- (9Z)-octadecenoyl-CoA + 1-octadecanoyl-sn-glycero-3-phospho-(1'-sn-glycerol) = 1-octadecanoyl-2-(9Z-octadecenoyl)-sn-glycero-3-phospho-(1'-sn-glycerol) + CoA1 PublicationThis reaction proceeds in the forward1 Publication direction.
- (5Z,8Z,11Z,14Z)-eicosatetraenoyl-CoA + 1-octadecanoyl-sn-glycero-3-phospho-(1'-sn-glycerol) = 1-octadecanoyl-2-(5Z,8Z,11Z,14Z-eicosatetraenoyl)-sn-glycero-3-phospho-(1ʼ-sn-glycerol) + CoA1 PublicationThis reaction proceeds in the forward1 Publication direction.
Kineticsi
- KM=80 µM for lysophosphatidylethanolamine1 Publication
- KM=20 µM for oleoyl-CoA1 Publication
- Vmax=270 pmol/min/mg enzyme with lysophosphatidylethanolamine and oleoyl-CoA as substrates1 Publication
: phospholipid metabolism Pathwayi
This protein is involved in the pathway phospholipid metabolism, which is part of Lipid metabolism.View all proteins of this organism that are known to be involved in the pathway phospholipid metabolism and in Lipid metabolism.
GO - Molecular functioni
- 1-acylglycerophosphocholine O-acyltransferase activity Source: UniProtKB
- 1-acylglycerophosphoethanolamine O-acyltransferase activity Source: UniProtKB
- 1-acylglycerophosphoserine O-acyltransferase activity Source: UniProtKB
- 1-alkenylglycerophosphoethanolamine O-acyltransferase activity Source: UniProtKB
- 1-alkylglycerophosphocholine O-acetyltransferase activity Source: UniProtKB
- lysophospholipid acyltransferase activity Source: MGI
GO - Biological processi
- phosphatidic acid biosynthetic process Source: Reactome
- phosphatidylcholine acyl-chain remodeling Source: UniProtKB
- phosphatidylethanolamine acyl-chain remodeling Source: UniProtKB
- phosphatidylglycerol acyl-chain remodeling Source: Reactome
- phosphatidylserine acyl-chain remodeling Source: UniProtKB
- phospholipid metabolic process Source: MGI
Keywordsi
Molecular function | Acyltransferase, Transferase |
Biological process | Lipid biosynthesis, Lipid metabolism, Phospholipid biosynthesis, Phospholipid metabolism |
Enzyme and pathway databases
BioCyci | MetaCyc:HS16664-MONOMER |
BRENDAi | 2.3.1.23, 2681 2.3.1.51, 2681 |
PathwayCommonsi | Q643R3 |
Reactomei | R-HSA-1482788, Acyl chain remodelling of PC R-HSA-1482801, Acyl chain remodelling of PS R-HSA-1482839, Acyl chain remodelling of PE R-HSA-1482925, Acyl chain remodelling of PG R-HSA-1483166, Synthesis of PA |
UniPathwayi | UPA00085 |
Chemistry databases
SwissLipidsi | SLP:000000294 |
Names & Taxonomyi
Protein namesi | Recommended name: Lysophospholipid acyltransferase LPCAT4Alternative name(s): 1-acylglycerol-3-phosphate O-acyltransferase 7 Short name: 1-AGP acyltransferase 7 Short name: 1-AGPAT 7 1-acylglycerophosphocholine O-acyltransferase (EC:2.3.1.231 Publication) 1-acylglycerophosphoserine O-acyltransferase (EC:2.3.1.n61 Publication) 1-alkenylglycerophosphoethanolamine O-acyltransferase (EC:2.3.1.1211 Publication) 1-alkylglycerophosphocholine O-acetyltransferase (EC:2.3.1.671 Publication) Acyltransferase-like 3 Lysophosphatidylcholine acyltransferase 4 Lysophosphatidylethanolamine acyltransferase 2 (EC:2.3.1.n71 Publication) Plasmalogen synthase |
Gene namesi | Name:LPCAT4 Synonyms:AGPAT7, AYTL3, LPEAT2 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:30059, LPCAT4 |
MIMi | 612039, gene |
neXtProti | NX_Q643R3 |
VEuPathDBi | HostDB:ENSG00000176454.13 |
Subcellular locationi
Endoplasmic reticulum
- Endoplasmic reticulum membrane 2 Publications; Multi-pass membrane protein 2 Publications
Endoplasmic reticulum
- endoplasmic reticulum Source: MGI
- endoplasmic reticulum membrane Source: Reactome
Other locations
- integral component of membrane Source: UniProtKB-KW
- membrane Source: UniProtKB
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transmembranei | 40 – 62 | HelicalSequence analysisAdd BLAST | 23 | |
Transmembranei | 87 – 107 | HelicalSequence analysisAdd BLAST | 21 |
Keywords - Cellular componenti
Endoplasmic reticulum, MembranePathology & Biotechi
Organism-specific databases
DisGeNETi | 254531 |
OpenTargetsi | ENSG00000176454 |
PharmGKBi | PA162394297 |
Miscellaneous databases
Pharosi | Q643R3, Tdark |
Genetic variation databases
BioMutai | LPCAT4 |
DMDMi | 74736281 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000247054 | 1 – 524 | Lysophospholipid acyltransferase LPCAT4Add BLAST | 524 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Glycosylationi | 152 | N-linked (GlcNAc...) asparagineSequence analysis | 1 |
Keywords - PTMi
GlycoproteinProteomic databases
EPDi | Q643R3 |
jPOSTi | Q643R3 |
MassIVEi | Q643R3 |
MaxQBi | Q643R3 |
PaxDbi | Q643R3 |
PeptideAtlasi | Q643R3 |
PRIDEi | Q643R3 |
ProteomicsDBi | 65914 |
PTM databases
GlyGeni | Q643R3, 1 site |
iPTMneti | Q643R3 |
MetOSitei | Q643R3 |
PhosphoSitePlusi | Q643R3 |
SwissPalmi | Q643R3 |
Expressioni
Tissue specificityi
Widely expressed with predominant level in brain.2 Publications
Gene expression databases
Bgeei | ENSG00000176454, Expressed in right hemisphere of cerebellum and 239 other tissues |
ExpressionAtlasi | Q643R3, baseline and differential |
Genevisiblei | Q643R3, HS |
Organism-specific databases
HPAi | ENSG00000176454, Low tissue specificity |
Interactioni
Protein-protein interaction databases
BioGRIDi | 129039, 26 interactors |
IntActi | Q643R3, 11 interactors |
STRINGi | 9606.ENSP00000317300 |
Miscellaneous databases
RNActi | Q643R3, protein |
Family & Domainsi
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 129 – 134 | HXXXXD motif | 6 |
Sequence similaritiesi
Belongs to the 1-acyl-sn-glycerol-3-phosphate acyltransferase family.Curated
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG4666, Eukaryota |
GeneTreei | ENSGT01000000214446 |
HOGENOMi | CLU_025017_1_0_1 |
InParanoidi | Q643R3 |
OMAi | QDGFSTI |
OrthoDBi | 1266853at2759 |
PhylomeDBi | Q643R3 |
TreeFami | TF323244 |
Family and domain databases
InterProi | View protein in InterPro IPR002123, Plipid/glycerol_acylTrfase |
Pfami | View protein in Pfam PF01553, Acyltransferase, 1 hit |
SMARTi | View protein in SMART SM00563, PlsC, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 2 potential isoforms that are computationally mapped.Show allAlign All
Q643R3-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSQGSPGDWA PLDPTPGPPA SPNPFVHELH LSRLQRVKFC LLGALLAPIR
60 70 80 90 100
VLLAFIVLFL LWPFAWLQVA GLSEEQLQEP ITGWRKTVCH NGVLGLSRLL
110 120 130 140 150
FFLLGFLRIR VRGQRASRLQ APVLVAAPHS TFFDPIVLLP CDLPKVVSRA
160 170 180 190 200
ENLSVPVIGA LLRFNQAILV SRHDPASRRR VVEEVRRRAT SGGKWPQVLF
210 220 230 240 250
FPEGTCSNKK ALLKFKPGAF IAGVPVQPVL IRYPNSLDTT SWAWRGPGVL
260 270 280 290 300
KVLWLTASQP CSIVDVEFLP VYHPSPEESR DPTLYANNVQ RVMAQALGIP
310 320 330 340 350
ATECEFVGSL PVIVVGRLKV ALEPQLWELG KVLRKAGLSA GYVDAGAEPG
360 370 380 390 400
RSRMISQEEF ARQLQLSDPQ TVAGAFGYFQ QDTKGLVDFR DVALALAALD
410 420 430 440 450
GGRSLEELTR LAFELFAEEQ AEGPNRLLYK DGFSTILHLL LGSPHPAATA
460 470 480 490 500
LHAELCQAGS SQGLSLCQFQ NFSLHDPLYG KLFSTYLRPP HTSRGTSQTP
510 520
NASSPGNPTA LANGTVQAPK QKGD
Computationally mapped potential isoform sequencesi
There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketA0A0C4DGT4 | A0A0C4DGT4_HUMAN | Lysophospholipid acyltransferase LP... | LPCAT4 | 351 | Annotation score: | ||
H3BMK4 | H3BMK4_HUMAN | Lysophospholipid acyltransferase LP... | LPCAT4 | 84 | Annotation score: |
Sequence cautioni
The sequence AAC19156 differs from that shown. Reason: Frameshift.Curated
The sequence AAN33178 differs from that shown. Reason: Frameshift.Curated
The sequence AAP97722 differs from that shown. Reason: Frameshift.Curated
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AY734233 mRNA Translation: AAU34184.1 AF529233 mRNA Translation: AAP97722.1 Frameshift. AF542964 mRNA Translation: AAN33178.1 Frameshift. AK290273 mRNA Translation: BAF82962.1 CH471125 Genomic DNA Translation: EAW92306.1 BC024892 mRNA Translation: AAH24892.2 BC092463 mRNA Translation: AAH92463.1 AF007155 mRNA Translation: AAC19156.1 Sequence problems. |
CCDSi | CCDS32191.1 |
RefSeqi | NP_705841.2, NM_153613.2 |
Genome annotation databases
Ensembli | ENST00000314891; ENSP00000317300; ENSG00000176454 |
GeneIDi | 254531 |
KEGGi | hsa:254531 |
UCSCi | uc001zig.4, human |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AY734233 mRNA Translation: AAU34184.1 AF529233 mRNA Translation: AAP97722.1 Frameshift. AF542964 mRNA Translation: AAN33178.1 Frameshift. AK290273 mRNA Translation: BAF82962.1 CH471125 Genomic DNA Translation: EAW92306.1 BC024892 mRNA Translation: AAH24892.2 BC092463 mRNA Translation: AAH92463.1 AF007155 mRNA Translation: AAC19156.1 Sequence problems. |
CCDSi | CCDS32191.1 |
RefSeqi | NP_705841.2, NM_153613.2 |
3D structure databases
SMRi | Q643R3 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 129039, 26 interactors |
IntActi | Q643R3, 11 interactors |
STRINGi | 9606.ENSP00000317300 |
Chemistry databases
SwissLipidsi | SLP:000000294 |
PTM databases
GlyGeni | Q643R3, 1 site |
iPTMneti | Q643R3 |
MetOSitei | Q643R3 |
PhosphoSitePlusi | Q643R3 |
SwissPalmi | Q643R3 |
Genetic variation databases
BioMutai | LPCAT4 |
DMDMi | 74736281 |
Proteomic databases
EPDi | Q643R3 |
jPOSTi | Q643R3 |
MassIVEi | Q643R3 |
MaxQBi | Q643R3 |
PaxDbi | Q643R3 |
PeptideAtlasi | Q643R3 |
PRIDEi | Q643R3 |
ProteomicsDBi | 65914 |
Protocols and materials databases
Antibodypediai | 22726, 54 antibodies |
Genome annotation databases
Ensembli | ENST00000314891; ENSP00000317300; ENSG00000176454 |
GeneIDi | 254531 |
KEGGi | hsa:254531 |
UCSCi | uc001zig.4, human |
Organism-specific databases
CTDi | 254531 |
DisGeNETi | 254531 |
GeneCardsi | LPCAT4 |
HGNCi | HGNC:30059, LPCAT4 |
HPAi | ENSG00000176454, Low tissue specificity |
MIMi | 612039, gene |
neXtProti | NX_Q643R3 |
OpenTargetsi | ENSG00000176454 |
PharmGKBi | PA162394297 |
VEuPathDBi | HostDB:ENSG00000176454.13 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG4666, Eukaryota |
GeneTreei | ENSGT01000000214446 |
HOGENOMi | CLU_025017_1_0_1 |
InParanoidi | Q643R3 |
OMAi | QDGFSTI |
OrthoDBi | 1266853at2759 |
PhylomeDBi | Q643R3 |
TreeFami | TF323244 |
Enzyme and pathway databases
UniPathwayi | UPA00085 |
BioCyci | MetaCyc:HS16664-MONOMER |
BRENDAi | 2.3.1.23, 2681 2.3.1.51, 2681 |
PathwayCommonsi | Q643R3 |
Reactomei | R-HSA-1482788, Acyl chain remodelling of PC R-HSA-1482801, Acyl chain remodelling of PS R-HSA-1482839, Acyl chain remodelling of PE R-HSA-1482925, Acyl chain remodelling of PG R-HSA-1483166, Synthesis of PA |
Miscellaneous databases
BioGRID-ORCSi | 254531, 5 hits in 990 CRISPR screens |
ChiTaRSi | LPCAT4, human |
GenomeRNAii | 254531 |
Pharosi | Q643R3, Tdark |
PROi | PR:Q643R3 |
RNActi | Q643R3, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000176454, Expressed in right hemisphere of cerebellum and 239 other tissues |
ExpressionAtlasi | Q643R3, baseline and differential |
Genevisiblei | Q643R3, HS |
Family and domain databases
InterProi | View protein in InterPro IPR002123, Plipid/glycerol_acylTrfase |
Pfami | View protein in Pfam PF01553, Acyltransferase, 1 hit |
SMARTi | View protein in SMART SM00563, PlsC, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | LPCT4_HUMAN | |
Accessioni | Q643R3Primary (citable) accession number: Q643R3 Secondary accession number(s): A8K2K8 Q8TB38 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 25, 2006 |
Last sequence update: | October 25, 2004 | |
Last modified: | April 7, 2021 | |
This is version 153 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Human chromosome 15
Human chromosome 15: entries, gene names and cross-references to MIM - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families