UniProtKB - Q64368 (DAZL_MOUSE)
Protein
Deleted in azoospermia-like
Gene
Dazl
Organism
Mus musculus (Mouse)
Status
Functioni
RNA-binding protein, which is essential for gametogenesis in both males and females. Plays a central role during spermatogenesis. Acts by binding to the 3'-UTR of mRNA, specifically recognizing GUU triplets, and thereby regulating the translation of key transcripts.2 Publications
GO - Molecular functioni
- identical protein binding Source: IntAct
- mRNA 3'-UTR binding Source: MGI
- translation activator activity Source: UniProtKB
GO - Biological processi
- 3'-UTR-mediated mRNA stabilization Source: GO_Central
- female meiosis II Source: MGI
- germ cell development Source: MGI
- multicellular organism development Source: UniProtKB-KW
- oocyte maturation Source: MGI
- positive regulation of meiotic nuclear division Source: MGI
- positive regulation of translational initiation Source: UniProtKB
- spermatogenesis Source: UniProtKB-KW
Keywordsi
Molecular function | Developmental protein, RNA-binding |
Biological process | Differentiation, Oogenesis, Spermatogenesis, Translation regulation |
Names & Taxonomyi
Protein namesi | Recommended name: Deleted in azoospermia-likeAlternative name(s): DAZ-like autosomal Deleted in azoospermia-like 1 |
Gene namesi | Name:Dazl Synonyms:Dazl1, Dazla |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1342328, Dazl |
Subcellular locationi
Other locations
- Cytoplasm 1 Publication
Nucleus
- nucleus Source: UniProtKB
Other locations
- cytoplasm Source: UniProtKB
- polysome Source: MGI
- protein-containing complex Source: MGI
Keywords - Cellular componenti
CytoplasmPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 43 | F → D: Abolishes RNA-binding but not homodimerization; when associated with D-82; D-84 and D-87. 1 Publication | 1 | |
Mutagenesisi | 82 | Y → D: Abolishes RNA-binding but not homodimerization; when associated with D-43; D-84 and D-87. 1 Publication | 1 | |
Mutagenesisi | 84 | F → D: Abolishes RNA-binding but not homodimerization; when associated with D-43; D-82 and D-87. 1 Publication | 1 | |
Mutagenesisi | 87 | F → D: Abolishes RNA-binding but not homodimerization; when associated with D-43; D-82 and D-84. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000081561 | 1 – 298 | Deleted in azoospermia-likeAdd BLAST | 298 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 276 | Phosphotyrosine1 Publication | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
PaxDbi | Q64368 |
PRIDEi | Q64368 |
PTM databases
iPTMneti | Q64368 |
PhosphoSitePlusi | Q64368 |
Expressioni
Tissue specificityi
Expressed predominantly in testis with lower levels in ovary. In testis, it is expressed in pachytene spermatocytes and at lower level in type-B spermatogonia, preleptotene and zygotene spermatocytes. In ovary, it is expressed in maturing follicles. In embryonic and prepuberal ovary, it is expressed in the oocyte and follicular cells.1 Publication
Developmental stagei
In the testis, expression increases steadily after birth until the first spermatogonial cells appear, levels off as the first spermatogenic cells enter meiosis (10 days after birth) and remains at this level thereafter.
Gene expression databases
Bgeei | ENSMUSG00000010592, Expressed in egg cell and 83 other tissues |
ExpressionAtlasi | Q64368, baseline and differential |
Genevisiblei | Q64368, MM |
Interactioni
Subunit structurei
Homodimer and heterodimer.
Forms a heterodimer with DAZ.
Interacts with BOLL, DAZAP1 and DAZAP2.
Interacts with PUM2 (By similarity). Multiple DAZL RRMs can bind to a single RNA containing multiple GUU triplets.
By similarity2 PublicationsBinary interactionsi
Hide detailsQ64368
With | #Exp. | IntAct |
---|---|---|
itself | 2 | EBI-2024439,EBI-2024439 |
Dync1i1 [O88485] | 4 | EBI-2024439,EBI-492834 |
Dynll1 [P63168] | 12 | EBI-2024439,EBI-349121 |
GO - Molecular functioni
- identical protein binding Source: IntAct
Protein-protein interaction databases
IntActi | Q64368, 6 interactors |
MINTi | Q64368 |
STRINGi | 10090.ENSMUSP00000010736 |
Miscellaneous databases
RNActi | Q64368, protein |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | Q64368 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | Q64368 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 40 – 115 | RRMPROSITE-ProRule annotationAdd BLAST | 76 | |
Domaini | 167 – 190 | DAZPROSITE-ProRule annotationAdd BLAST | 24 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 80 – 132 | HomodimerizationAdd BLAST | 53 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 126 – 207 | Pro-richAdd BLAST | 82 |
Domaini
The DAZ domain mediates the interaction with DAZAP1 and DAZAP2.By similarity
Sequence similaritiesi
Belongs to the RRM DAZ family.PROSITE-ProRule annotation
Phylogenomic databases
eggNOGi | KOG0118, Eukaryota |
GeneTreei | ENSGT00530000063480 |
HOGENOMi | CLU_084802_0_0_1 |
InParanoidi | Q64368 |
OMAi | QSECSVH |
OrthoDBi | 1610446at2759 |
PhylomeDBi | Q64368 |
TreeFami | TF324396 |
Family and domain databases
CDDi | cd12672, RRM_DAZL, 1 hit |
Gene3Di | 3.30.70.330, 1 hit |
InterProi | View protein in InterPro IPR037366, BOULE/DAZ IPR043628, DAZ_dom IPR037551, DAZ_RRM_vert IPR034779, DAZL IPR012677, Nucleotide-bd_a/b_plait_sf IPR035979, RBD_domain_sf IPR000504, RRM_dom |
PANTHERi | PTHR11176, PTHR11176, 1 hit PTHR11176:SF4, PTHR11176:SF4, 1 hit |
Pfami | View protein in Pfam PF18872, Daz, 1 hit PF00076, RRM_1, 1 hit |
SMARTi | View protein in SMART SM00360, RRM, 1 hit |
SUPFAMi | SSF54928, SSF54928, 1 hit |
PROSITEi | View protein in PROSITE PS51890, DAZ, 1 hit PS50102, RRM, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 2 potential isoforms that are computationally mapped.Show allAlign All
Q64368-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSATTSEAPN SAVSREASTQ SSSATTSQGY VLPEGKIMPN TVFVGGIDVR
60 70 80 90 100
MDETEIRSFF ARYGSVKEVK IITDRTGVSK GYGFVSFYND VDVQKIVESQ
110 120 130 140 150
INFHGKKLKL GPAIRKQNLC TYHVQPRPLI FNPPPPPQFQ SVWSSPNAET
160 170 180 190 200
YMQPPTMMNP ITQYVQAYPP YPSSPVQVIT GYQLPVYNYQ MPPQWPAGEQ
210 220 230 240 250
RSYVIPPAYT TVNYHCSEVD PGADILPNEC SVHDAAPASG NGPQKKSVDR
260 270 280 290
SIQTVVSCLF NPENRLRNSL VTQDDYFKDK RVHHFRRSRA VLKSDHLC
Computationally mapped potential isoform sequencesi
There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketQ3TUC3 | Q3TUC3_MOUSE | Deleted in azoospermia-like | Dazl | 261 | Annotation score: | ||
A0A3B2WD26 | A0A3B2WD26_MOUSE | Deleted in azoospermia-like | Dazl | 133 | Annotation score: |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X95724 mRNA Translation: CAA65039.1 U38690 mRNA Translation: AAB46608.1 U46694 mRNA Translation: AAC52711.1 AK014854 mRNA Translation: BAB29585.1 AK033183 mRNA Translation: BAC28187.1 |
CCDSi | CCDS37649.1 |
RefSeqi | NP_001264792.1, NM_001277863.1 NP_034151.3, NM_010021.5 |
Genome annotation databases
Ensembli | ENSMUST00000010736; ENSMUSP00000010736; ENSMUSG00000010592 |
GeneIDi | 13164 |
KEGGi | mmu:13164 |
UCSCi | uc008cyu.3, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X95724 mRNA Translation: CAA65039.1 U38690 mRNA Translation: AAB46608.1 U46694 mRNA Translation: AAC52711.1 AK014854 mRNA Translation: BAB29585.1 AK033183 mRNA Translation: BAC28187.1 |
CCDSi | CCDS37649.1 |
RefSeqi | NP_001264792.1, NM_001277863.1 NP_034151.3, NM_010021.5 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
2XS2 | X-ray | 1.35 | A | 32-132 | [»] | |
2XS5 | X-ray | 1.60 | A/B | 32-117 | [»] | |
2XS7 | X-ray | 1.45 | A | 32-117 | [»] | |
2XSF | X-ray | 1.70 | A | 35-118 | [»] | |
SMRi | Q64368 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
IntActi | Q64368, 6 interactors |
MINTi | Q64368 |
STRINGi | 10090.ENSMUSP00000010736 |
PTM databases
iPTMneti | Q64368 |
PhosphoSitePlusi | Q64368 |
Proteomic databases
PaxDbi | Q64368 |
PRIDEi | Q64368 |
Protocols and materials databases
Antibodypediai | 6122, 309 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000010736; ENSMUSP00000010736; ENSMUSG00000010592 |
GeneIDi | 13164 |
KEGGi | mmu:13164 |
UCSCi | uc008cyu.3, mouse |
Organism-specific databases
CTDi | 1618 |
MGIi | MGI:1342328, Dazl |
Phylogenomic databases
eggNOGi | KOG0118, Eukaryota |
GeneTreei | ENSGT00530000063480 |
HOGENOMi | CLU_084802_0_0_1 |
InParanoidi | Q64368 |
OMAi | QSECSVH |
OrthoDBi | 1610446at2759 |
PhylomeDBi | Q64368 |
TreeFami | TF324396 |
Miscellaneous databases
BioGRID-ORCSi | 13164, 1 hit in 17 CRISPR screens |
EvolutionaryTracei | Q64368 |
PROi | PR:Q64368 |
RNActi | Q64368, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000010592, Expressed in egg cell and 83 other tissues |
ExpressionAtlasi | Q64368, baseline and differential |
Genevisiblei | Q64368, MM |
Family and domain databases
CDDi | cd12672, RRM_DAZL, 1 hit |
Gene3Di | 3.30.70.330, 1 hit |
InterProi | View protein in InterPro IPR037366, BOULE/DAZ IPR043628, DAZ_dom IPR037551, DAZ_RRM_vert IPR034779, DAZL IPR012677, Nucleotide-bd_a/b_plait_sf IPR035979, RBD_domain_sf IPR000504, RRM_dom |
PANTHERi | PTHR11176, PTHR11176, 1 hit PTHR11176:SF4, PTHR11176:SF4, 1 hit |
Pfami | View protein in Pfam PF18872, Daz, 1 hit PF00076, RRM_1, 1 hit |
SMARTi | View protein in SMART SM00360, RRM, 1 hit |
SUPFAMi | SSF54928, SSF54928, 1 hit |
PROSITEi | View protein in PROSITE PS51890, DAZ, 1 hit PS50102, RRM, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | DAZL_MOUSE | |
Accessioni | Q64368Primary (citable) accession number: Q64368 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | January 17, 2003 |
Last sequence update: | November 1, 1996 | |
Last modified: | December 2, 2020 | |
This is version 168 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families