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UniProtKB - Q64350 (EI2BE_RAT)
Protein
Translation initiation factor eIF-2B subunit epsilon
Gene
Eif2b5
Organism
Rattus norvegicus (Rat)
Status
Functioni
Catalyzes the exchange of eukaryotic initiation factor 2-bound GDP for GTP.
GO - Molecular functioni
- guanyl-nucleotide exchange factor activity Source: MGI
- translation initiation factor activity Source: GO_Central
- translation initiation factor binding Source: RGD
GO - Biological processi
- aging Source: RGD
- astrocyte development Source: UniProtKB
- astrocyte differentiation Source: UniProtKB
- hippocampus development Source: RGD
- myelination Source: UniProtKB
- oligodendrocyte development Source: UniProtKB
- ovarian follicle development Source: UniProtKB
- positive regulation of apoptotic process Source: RGD
- positive regulation of translation Source: RGD
- positive regulation of translational initiation Source: UniProtKB
- response to endoplasmic reticulum stress Source: UniProtKB
- response to glucose Source: UniProtKB
- response to heat Source: UniProtKB
- response to lithium ion Source: RGD
- response to peptide hormone Source: UniProtKB
- T cell receptor signaling pathway Source: UniProtKB
- translational initiation Source: UniProtKB
Keywordsi
Molecular function | Initiation factor |
Biological process | Protein biosynthesis |
Enzyme and pathway databases
Reactomei | R-RNO-72731, Recycling of eIF2:GDP |
Names & Taxonomyi
Protein namesi | Recommended name: Translation initiation factor eIF-2B subunit epsilonAlternative name(s): eIF-2B GDP-GTP exchange factor subunit epsilon |
Gene namesi | Name:Eif2b5 Synonyms:Eif2be |
Organismi | Rattus norvegicus (Rat) |
Taxonomic identifieri | 10116 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Rattus |
Proteomesi |
|
Organism-specific databases
RGDi | 708380, Eif2b5 |
Subcellular locationi
Cytosol
- cytosol Source: Ensembl
Nucleus
- nucleus Source: RGD
Other locations
- cytoplasm Source: UniProtKB
- eukaryotic translation initiation factor 2B complex Source: RGD
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000156075 | 1 – 716 | Translation initiation factor eIF-2B subunit epsilonAdd BLAST | 716 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 18 | Omega-N-methylarginineBy similarity | 1 | |
Modified residuei | 22 | Phosphoserine1 Publication | 1 | |
Cross-linki | 56 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)1 Publication | ||
Cross-linki | 98 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)1 Publication | ||
Modified residuei | 125 | Phosphoserine1 Publication | 1 | |
Cross-linki | 136 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)1 Publication | ||
Cross-linki | 212 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)1 Publication | ||
Modified residuei | 317 | Phosphothreonine1 Publication | 1 | |
Modified residuei | 445 | PhosphoserineBy similarity | 1 | |
Modified residuei | 461 | PhosphoserineBy similarity | 1 | |
Modified residuei | 464 | PhosphoserineBy similarity | 1 | |
Cross-linki | 500 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)1 Publication | ||
Modified residuei | 527 | PhosphoserineCombined sources | 1 | |
Modified residuei | 535 | PhosphoserineCombined sources | 1 | |
Modified residuei | 539 | Phosphoserine; by DYRK2Combined sources1 Publication | 1 | |
Modified residuei | 712 | PhosphoserineBy similarity | 1 |
Post-translational modificationi
Phosphorylated on serine and threonine residues by GSK3B; phosphorylation inhibits its function (By similarity). Phosphorylated at Ser-539 by DYRK2; this is required for subsequent phosphorylation by GSK3B.By similarity2 Publications
Polyubiquitinated, probably by NEDD4.1 Publication
Keywords - PTMi
Isopeptide bond, Methylation, Phosphoprotein, Ubl conjugationProteomic databases
jPOSTi | Q64350 |
PaxDbi | Q64350 |
PTM databases
iPTMneti | Q64350 |
PhosphoSitePlusi | Q64350 |
Expressioni
Gene expression databases
Bgeei | ENSRNOG00000038160, Expressed in skeletal muscle tissue and 22 other tissues |
Genevisiblei | Q64350, RN |
Interactioni
Subunit structurei
Complex of five different subunits; alpha, beta, gamma, delta and epsilon.
Interacts with RGS2 (By similarity).
By similarityGO - Molecular functioni
- translation initiation factor binding Source: RGD
Protein-protein interaction databases
BioGRIDi | 251366, 4 interactors |
IntActi | Q64350, 2 interactors |
STRINGi | 10116.ENSRNOP00000002321 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 538 – 715 | W2PROSITE-ProRule annotationAdd BLAST | 178 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 35 | DisorderedSequence analysisAdd BLAST | 35 | |
Regioni | 443 – 481 | DisorderedSequence analysisAdd BLAST | 39 | |
Regioni | 517 – 539 | DisorderedSequence analysisAdd BLAST | 23 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 463 – 477 | Basic and acidic residuesSequence analysisAdd BLAST | 15 |
Sequence similaritiesi
Belongs to the eIF-2B gamma/epsilon subunits family.Curated
Phylogenomic databases
eggNOGi | KOG1461, Eukaryota |
GeneTreei | ENSGT00510000047568 |
HOGENOMi | CLU_012507_2_0_1 |
InParanoidi | Q64350 |
OMAi | HYLYDKD |
OrthoDBi | 369837at2759 |
PhylomeDBi | Q64350 |
TreeFami | TF101509 |
Family and domain databases
CDDi | cd04197, eIF-2B_epsilon_N, 1 hit cd11558, W2_eIF2B_epsilon, 1 hit |
Gene3Di | 3.90.550.10, 1 hit |
InterProi | View protein in InterPro IPR016024, ARM-type_fold IPR035543, eIF-2B_epsilon_N IPR001451, Hexapep IPR029044, Nucleotide-diphossugar_trans IPR011004, Trimer_LpxA-like_sf IPR003307, W2_domain IPR044123, W2_eIF2B_epsilon |
Pfami | View protein in Pfam PF00132, Hexapep, 1 hit PF02020, W2, 1 hit |
SMARTi | View protein in SMART SM00515, eIF5C, 1 hit |
SUPFAMi | SSF48371, SSF48371, 1 hit SSF51161, SSF51161, 1 hit SSF53448, SSF53448, 1 hit |
PROSITEi | View protein in PROSITE PS51363, W2, 1 hit |
i Sequence
Sequence statusi: Complete.
Q64350-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MAATAAVPSA VGGRANKRGG GSGGGGTQGA EEEPPPPLQA VLVADSFDRR
60 70 80 90 100
FFPISKDQPR VLLPLANVAL IDYTLEFLTA TGVQETFVFC CWKAAQIKEH
110 120 130 140 150
LQKSKWCHPT SLNVVRIITS DLYRSLGDVL RDVDAKALVR SDFLLIYGDV
160 170 180 190 200
VSNINISKAL EEHRLRRKLE KNVSVMTMVF KESSPSHPTR CHEDNVVLAV
210 220 230 240 250
DSTTNRILHF QKTQGLRHFS FPLGLFQGSL DGVEIRYDLL DCHISICSPQ
260 270 280 290 300
VAQLFTDNFD YQTRDDFVRG LLVNEEILGN QIHLHVTSRE YGARVSNLHM
310 320 330 340 350
YSAVCADVIR RWVYPLTPEV NFTDSSTQSY THSRHNIYRG PEVSLGHGSV
360 370 380 390 400
LEENVLLGAG TVVGSNCSIT NSVIGPNCHI GDNVVLDQAY LWQGVRVAAG
410 420 430 440 450
AQIHQSLLCD RAEVKERVIL KPHCVLTSQV VVGPDIILPE GSVISLHPPD
460 470 480 490 500
AEEDEDDGQF SDDSGADQEK EKVKLKGYNP AEVGPEGQGY LWKAEDVDEK
510 520 530 540 550
EDEELRQSLW GLMINMEEES ETESERSVDP EELDSRAGSP QLDDIRVFQN
560 570 580 590 600
EVLGTLQRGR EENISCDNLV LEINSLKYAY NISLKEVMQV LSHVVLEFPL
610 620 630 640 650
QQVDGVLDPN RYCALLLPLL KAWSPVFRNY IKRAADHLEA LAAIEDFFLE
660 670 680 690 700
HETLVPSLAK VLMAFYQLEI LAEETILSWF SQRDITDKGQ QLRKNQQLQR
710
FIQWLREAEE ESSDDD
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 118 | I → T in AAB17690 (PubMed:8688467).Curated | 1 | |
Sequence conflicti | 118 | I → T in AAB17691 (PubMed:8688467).Curated | 1 | |
Sequence conflicti | 293 | A → S in AAB17690 (PubMed:8688467).Curated | 1 | |
Sequence conflicti | 293 | A → S in AAB17691 (PubMed:8688467).Curated | 1 | |
Sequence conflicti | 306 | A → T in AAB17690 (PubMed:8688467).Curated | 1 | |
Sequence conflicti | 306 | A → T in AAB17691 (PubMed:8688467).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U19516 mRNA Translation: AAB17690.1 U19511 Genomic DNA Translation: AAB17691.1 BC085698 mRNA Translation: AAH85698.1 |
RefSeqi | NP_620221.2, NM_138866.2 |
Genome annotation databases
Ensembli | ENSRNOT00000002321; ENSRNOP00000002321; ENSRNOG00000038160 |
GeneIDi | 192234 |
KEGGi | rno:192234 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U19516 mRNA Translation: AAB17690.1 U19511 Genomic DNA Translation: AAB17691.1 BC085698 mRNA Translation: AAH85698.1 |
RefSeqi | NP_620221.2, NM_138866.2 |
3D structure databases
AlphaFoldDBi | Q64350 |
SMRi | Q64350 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 251366, 4 interactors |
IntActi | Q64350, 2 interactors |
STRINGi | 10116.ENSRNOP00000002321 |
PTM databases
iPTMneti | Q64350 |
PhosphoSitePlusi | Q64350 |
Proteomic databases
jPOSTi | Q64350 |
PaxDbi | Q64350 |
Genome annotation databases
Ensembli | ENSRNOT00000002321; ENSRNOP00000002321; ENSRNOG00000038160 |
GeneIDi | 192234 |
KEGGi | rno:192234 |
Organism-specific databases
CTDi | 8893 |
RGDi | 708380, Eif2b5 |
Phylogenomic databases
eggNOGi | KOG1461, Eukaryota |
GeneTreei | ENSGT00510000047568 |
HOGENOMi | CLU_012507_2_0_1 |
InParanoidi | Q64350 |
OMAi | HYLYDKD |
OrthoDBi | 369837at2759 |
PhylomeDBi | Q64350 |
TreeFami | TF101509 |
Enzyme and pathway databases
Reactomei | R-RNO-72731, Recycling of eIF2:GDP |
Miscellaneous databases
PROi | PR:Q64350 |
Gene expression databases
Bgeei | ENSRNOG00000038160, Expressed in skeletal muscle tissue and 22 other tissues |
Genevisiblei | Q64350, RN |
Family and domain databases
CDDi | cd04197, eIF-2B_epsilon_N, 1 hit cd11558, W2_eIF2B_epsilon, 1 hit |
Gene3Di | 3.90.550.10, 1 hit |
InterProi | View protein in InterPro IPR016024, ARM-type_fold IPR035543, eIF-2B_epsilon_N IPR001451, Hexapep IPR029044, Nucleotide-diphossugar_trans IPR011004, Trimer_LpxA-like_sf IPR003307, W2_domain IPR044123, W2_eIF2B_epsilon |
Pfami | View protein in Pfam PF00132, Hexapep, 1 hit PF02020, W2, 1 hit |
SMARTi | View protein in SMART SM00515, eIF5C, 1 hit |
SUPFAMi | SSF48371, SSF48371, 1 hit SSF51161, SSF51161, 1 hit SSF53448, SSF53448, 1 hit |
PROSITEi | View protein in PROSITE PS51363, W2, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | EI2BE_RAT | |
Accessioni | Q64350Primary (citable) accession number: Q64350 Secondary accession number(s): Q5RKL3 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 1, 1997 |
Last sequence update: | April 4, 2006 | |
Last modified: | May 25, 2022 | |
This is version 145 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Direct protein sequencing, Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families