Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Chloride channel protein 1

Gene

Clcn1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Voltage-gated chloride channel (By similarity). Plays an important role in membrane repolarization in skeletal muscle cells after muscle contraction (Probable) (PubMed:8119941).By similarity1 Publication1 Publication

Miscellaneous

The CLC channel family contains both chloride channels and proton-coupled anion transporters that exchange chloride or another anion for protons. The absence of conserved gating glutamate residues is typical for family members that function as channels (By similarity).By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei189ChlorideBy similarity1
Binding sitei484Chloride; via amide nitrogenBy similarity1
Binding sitei578ChlorideBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionChloride channel, Ion channel, Voltage-gated channel
Biological processIon transport, Transport
LigandChloride

Enzyme and pathway databases

ReactomeiR-MMU-2672351 Stimuli-sensing channels

Names & Taxonomyi

Protein namesi
Recommended name:
Chloride channel protein 1
Short name:
ClC-1
Alternative name(s):
Chloride channel protein, skeletal muscle
Gene namesi
Name:Clcn1
Synonyms:Clc1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 6

Organism-specific databases

MGIiMGI:88417 Clcn1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 118CytoplasmicCuratedAdd BLAST118
Transmembranei119 – 150HelicalBy similarityAdd BLAST32
Topological domaini151 – 158ExtracellularCurated8
Transmembranei159 – 179HelicalBy similarityAdd BLAST21
Topological domaini180 – 183CytoplasmicCurated4
Intramembranei184 – 195HelicalBy similarityAdd BLAST12
Topological domaini196 – 208CytoplasmicCuratedAdd BLAST13
Transmembranei209 – 228HelicalBy similarityAdd BLAST20
Transmembranei229 – 246HelicalBy similarityAdd BLAST18
Topological domaini247 – 268CytoplasmicCuratedAdd BLAST22
Intramembranei269 – 290HelicalBy similarityAdd BLAST22
Topological domaini291 – 301CytoplasmicCuratedAdd BLAST11
Transmembranei302 – 321HelicalBy similarityAdd BLAST20
Topological domaini322 – 347ExtracellularCuratedAdd BLAST26
Transmembranei348 – 376HelicalBy similarityAdd BLAST29
Topological domaini377 – 390CytoplasmicCuratedAdd BLAST14
Transmembranei391 – 408HelicalBy similarityAdd BLAST18
Topological domaini409 – 414ExtracellularCurated6
Intramembranei415 – 426HelicalBy similarityAdd BLAST12
Topological domaini427 – 457ExtracellularCuratedAdd BLAST31
Transmembranei458 – 478HelicalBy similarityAdd BLAST21
Transmembranei479 – 498HelicalBy similarityAdd BLAST20
Topological domaini499 – 521ExtracellularCuratedAdd BLAST23
Intramembranei522 – 554HelicalBy similarityAdd BLAST33
Topological domaini555 – 557ExtracellularCurated3
Transmembranei558 – 578HelicalBy similarityAdd BLAST21
Topological domaini579 – 994CytoplasmicCuratedAdd BLAST416

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi553I → T: Causes myotonia. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000944301 – 994Chloride channel protein 1Add BLAST994

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei892PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ64347
PRIDEiQ64347

PTM databases

iPTMnetiQ64347
PhosphoSitePlusiQ64347

Expressioni

Tissue specificityi

Predominantly expressed in skeletal muscles.1 Publication

Gene expression databases

BgeeiENSMUSG00000029862 Expressed in 79 organ(s), highest expression level in skeletal muscle tissue
CleanExiMM_CLCN1
ExpressionAtlasiQ64347 baseline and differential

Interactioni

Subunit structurei

Homodimer.By similarity

GO - Molecular functioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000031894

Structurei

3D structure databases

ProteinModelPortaliQ64347
SMRiQ64347
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini609 – 668CBS 1PROSITE-ProRule annotationAdd BLAST60
Domaini827 – 882CBS 2PROSITE-ProRule annotationAdd BLAST56

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi188 – 192Selectivity filter part_1By similarity5
Motifi230 – 234Selectivity filter part_2By similarity5
Motifi482 – 486Selectivity filter part_3By similarity5

Sequence similaritiesi

Keywords - Domaini

CBS domain, Repeat, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0476 Eukaryota
COG0038 LUCA
GeneTreeiENSGT00760000119109
HOGENOMiHOG000231297
HOVERGENiHBG005332
InParanoidiQ64347
KOiK05010
OMAiHISKYNI
OrthoDBiEOG091G01RJ
PhylomeDBiQ64347
TreeFamiTF352264

Family and domain databases

Gene3Di1.10.3080.10, 1 hit
InterProiView protein in InterPro
IPR000644 CBS_dom
IPR014743 Cl-channel_core
IPR001807 Cl-channel_volt-gated
IPR002243 Cl_channel-1
PfamiView protein in Pfam
PF00654 Voltage_CLC, 1 hit
PRINTSiPR00762 CLCHANNEL
PR01112 CLCHANNEL1
SUPFAMiSSF81340 SSF81340, 1 hit
PROSITEiView protein in PROSITE
PS51371 CBS, 2 hits

Sequence (1+)i

Sequence statusi: Complete.

This entry has 1 described isoform and 7 potential isoforms that are computationally mapped.Show allAlign All

Q64347-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MERSQSQRHG GEQSWWGSAP QYQYMPFEHC TSYGLPSENG GLQHRPRKDM
60 70 80 90 100
GPRHNAHPTQ IYGHQKEQYS YKAQDGGMPK KMGSSSTMDS LDEDHYSKCQ
110 120 130 140 150
DCVHRLGRVL RRKLGEDWIF LVLLGLLMAL VSWCMDYVSA KSLQAYKWTY
160 170 180 190 200
AQMKPSLPLQ YLAWVTFPLI LILFSALFCQ LISPQAVGSG IPEMKTILRG
210 220 230 240 250
VVLKEYLTLK AFVAKVVALT AGLGSGIPVG KEGPFVHIAS ICAAVLSKFM
260 270 280 290 300
SMFSGVYEQP YYYTDILTVG CAVGVGCCFG TPLGGVLFSI EVTSTYFAVR
310 320 330 340 350
NYWRGFFAAT FSAFVFRVLA VWNKDAVTIT ALFRTNFRMD FPFDLKELPA
360 370 380 390 400
FAVIGICCGF LGAVFVYLHR QVMLGVRKHK CLSQFLAKHR LLYPGIVTFV
410 420 430 440 450
IASLTFPPGM GQFMAGELMP REAISTLFDN NTWVKHIGDP QSLGQSAVWL
460 470 480 490 500
HPQVNVIIII LLFFVMKFWM SIVATTMPIP CGGFMPVFVL GAAFGRLVGE
510 520 530 540 550
IMAMLFPEGI LFDDIIYKIL PGGYAVIGAA ALTGAVSHTV STAVICFELT
560 570 580 590 600
GQIAHILPMM VAVILANMVA QSLQPSLYDS IIQVKKLPYL PDLGWNQLSK
610 620 630 640 650
FTIFVEDIMV RDVKFVSASC TYGELRNLLQ ATTVKTLPLV DSKDSMILLG
660 670 680 690 700
SVERSELQSL LQRHLCAERR LKAAQDMARK LSELPYNGKA QLAGDWHPGG
710 720 730 740 750
RPESFAFVDE DEDEDLSRKM ELPLTPAPPP PSPPPPPSQF PIAPSNPEEP
760 770 780 790 800
NGPLPSHKQP PEASDSADQR SSTFQRLLHC LLGKAHSKKK KITQDSTDLV
810 820 830 840 850
DNMSPEEIEA WEREQLSQPV CFDCCCIDQS PFQLVEQTTL HKTHTLFSLL
860 870 880 890 900
GLHLAYVTSM GKLRGVLALE ELQKAIEGHT KSGVQLRPPL ASFRNTTSIR
910 920 930 940 950
KTPGGPPPPA EGWNVPEDGD GAPGREVMVP TMPETPVPPP SPEAPSCLAP
960 970 980 990
ARAEGELEEL EMVGSLEPEE ELADILHGPS LRSTDEEDED ELIL
Length:994
Mass (Da):109,794
Last modified:May 18, 2010 - v3
Checksum:i3C523C4325B279BF
GO

Computationally mapped potential isoform sequencesi

There are 7 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
F6QI82F6QI82_MOUSE
Chloride channel protein
Clcn1
923Annotation score:
E9Q2N3E9Q2N3_MOUSE
Chloride channel protein 1
Clcn1
258Annotation score:
F6X177F6X177_MOUSE
Chloride channel protein 1
Clcn1
289Annotation score:
F7A9J6F7A9J6_MOUSE
Chloride channel protein 1
Clcn1
257Annotation score:
F6QL23F6QL23_MOUSE
Chloride channel protein 1
Clcn1
229Annotation score:
F7C9S1F7C9S1_MOUSE
Chloride channel protein 1
Clcn1
262Annotation score:
F6VSA8F6VSA8_MOUSE
Chloride channel protein 1
Clcn1
104Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti65Q → H in CAB08359 (PubMed:9321463).Curated1
Sequence conflicti72K → Q in CAB08359 (PubMed:9321463).Curated1
Sequence conflicti76 – 78GGM → RGI in CAB08359 (PubMed:9321463).Curated3
Sequence conflicti82 – 83MG → TD in CAB08359 (PubMed:9321463).Curated2
Sequence conflicti88M → V in CAB08359 (PubMed:9321463).Curated1
Sequence conflicti243A → T in CAB08359 (PubMed:9321463).Curated1
Sequence conflicti483 – 484GF → VN in CAB08359 (PubMed:9321463).Curated2
Sequence conflicti585K → E in CAB08359 (PubMed:9321463).Curated1
Sequence conflicti620C → S in CAB08359 (PubMed:9321463).Curated1
Sequence conflicti631A → T in CAB08359 (PubMed:9321463).Curated1
Sequence conflicti746N → H no nucleotide entry (PubMed:8119941).Curated1
Sequence conflicti864R → T in CAB08359 (PubMed:9321463).Curated1
Sequence conflicti877E → K in CAB08359 (PubMed:9321463).Curated1
Sequence conflicti912G → S in CAB08359 (PubMed:9321463).Curated1
Sequence conflicti918 – 919DG → GE in CAB08359 (PubMed:9321463).Curated2
Sequence conflicti924G → E in CAB08359 (PubMed:9321463).Curated1
Sequence conflicti944A → V no nucleotide entry (PubMed:8119941).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z95127
, Z95128, Z95129, Z95130, Z95131, Z95132, Z95133, Z95134, Z95135, Z95136, Z95137, Z95138, Z95139, Z95140, Z95141, Z95142, Z95143, Z95144, Z95145, Z95146, Z95147, Z95148, Z95149 Genomic DNA Translation: CAB08359.1
AK036735 mRNA Translation: BAC29557.1
CH466533 Genomic DNA Translation: EDL13488.1
BC114336 mRNA Translation: AAI14337.1
X62895 mRNA Translation: CAA44684.1
X62896 mRNA Translation: CAA44685.1
X62897 mRNA Translation: CAA44686.1
CCDSiCCDS39471.1
PIRiI48773
RefSeqiNP_038519.1, NM_013491.2
UniGeneiMm.386757

Genome annotation databases

EnsembliENSMUST00000031894; ENSMUSP00000031894; ENSMUSG00000029862
GeneIDi12723
KEGGimmu:12723
UCSCiuc009bqs.1 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z95127
, Z95128, Z95129, Z95130, Z95131, Z95132, Z95133, Z95134, Z95135, Z95136, Z95137, Z95138, Z95139, Z95140, Z95141, Z95142, Z95143, Z95144, Z95145, Z95146, Z95147, Z95148, Z95149 Genomic DNA Translation: CAB08359.1
AK036735 mRNA Translation: BAC29557.1
CH466533 Genomic DNA Translation: EDL13488.1
BC114336 mRNA Translation: AAI14337.1
X62895 mRNA Translation: CAA44684.1
X62896 mRNA Translation: CAA44685.1
X62897 mRNA Translation: CAA44686.1
CCDSiCCDS39471.1
PIRiI48773
RefSeqiNP_038519.1, NM_013491.2
UniGeneiMm.386757

3D structure databases

ProteinModelPortaliQ64347
SMRiQ64347
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000031894

PTM databases

iPTMnetiQ64347
PhosphoSitePlusiQ64347

Proteomic databases

PaxDbiQ64347
PRIDEiQ64347

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000031894; ENSMUSP00000031894; ENSMUSG00000029862
GeneIDi12723
KEGGimmu:12723
UCSCiuc009bqs.1 mouse

Organism-specific databases

CTDi1180
MGIiMGI:88417 Clcn1

Phylogenomic databases

eggNOGiKOG0476 Eukaryota
COG0038 LUCA
GeneTreeiENSGT00760000119109
HOGENOMiHOG000231297
HOVERGENiHBG005332
InParanoidiQ64347
KOiK05010
OMAiHISKYNI
OrthoDBiEOG091G01RJ
PhylomeDBiQ64347
TreeFamiTF352264

Enzyme and pathway databases

ReactomeiR-MMU-2672351 Stimuli-sensing channels

Miscellaneous databases

PROiPR:Q64347
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000029862 Expressed in 79 organ(s), highest expression level in skeletal muscle tissue
CleanExiMM_CLCN1
ExpressionAtlasiQ64347 baseline and differential

Family and domain databases

Gene3Di1.10.3080.10, 1 hit
InterProiView protein in InterPro
IPR000644 CBS_dom
IPR014743 Cl-channel_core
IPR001807 Cl-channel_volt-gated
IPR002243 Cl_channel-1
PfamiView protein in Pfam
PF00654 Voltage_CLC, 1 hit
PRINTSiPR00762 CLCHANNEL
PR01112 CLCHANNEL1
SUPFAMiSSF81340 SSF81340, 1 hit
PROSITEiView protein in PROSITE
PS51371 CBS, 2 hits
ProtoNetiSearch...

Entry informationi

Entry nameiCLCN1_MOUSE
AccessioniPrimary (citable) accession number: Q64347
Secondary accession number(s): Q8BZ41, Q9QVY5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 21, 2001
Last sequence update: May 18, 2010
Last modified: November 7, 2018
This is version 147 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again