UniProtKB - Q64347 (CLCN1_MOUSE)
Protein
Chloride channel protein 1
Gene
Clcn1
Organism
Mus musculus (Mouse)
Status
Functioni
Voltage-gated chloride channel (By similarity). Plays an important role in membrane repolarization in skeletal muscle cells after muscle contraction (Probable) (PubMed:8119941).By similarity1 Publication1 Publication
Miscellaneous
The CLC channel family contains both chloride channels and proton-coupled anion transporters that exchange chloride or another anion for protons. The absence of conserved gating glutamate residues is typical for family members that function as channels (By similarity).By similarity
Catalytic activityi
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 189 | ChlorideBy similarity | 1 | |
Binding sitei | 484 | Chloride; via amide nitrogenBy similarity | 1 | |
Binding sitei | 578 | ChlorideBy similarity | 1 |
GO - Molecular functioni
- protein homodimerization activity Source: UniProtKB
- voltage-gated chloride channel activity Source: UniProtKB
GO - Biological processi
- chloride transmembrane transport Source: UniProtKB
- chloride transport Source: MGI
- muscle contraction Source: UniProtKB
- neuronal action potential propagation Source: MGI
- regulation of ion transmembrane transport Source: UniProtKB-KW
Keywordsi
Molecular function | Chloride channel, Ion channel, Voltage-gated channel |
Biological process | Ion transport, Transport |
Ligand | Chloride |
Enzyme and pathway databases
Reactomei | R-MMU-2672351, Stimuli-sensing channels |
Names & Taxonomyi
Protein namesi | Recommended name: Chloride channel protein 1Short name: ClC-1 Alternative name(s): Chloride channel protein, skeletal muscle |
Gene namesi | Name:Clcn1 Synonyms:Clc1 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:88417, Clcn1 |
Subcellular locationi
Plasma membrane
- Cell membrane By similarity; Multi-pass membrane protein By similarity
Plasma Membrane
- integral component of plasma membrane Source: UniProtKB
- sarcolemma Source: MGI
Other locations
- chloride channel complex Source: UniProtKB-KW
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 118 | CytoplasmicCuratedAdd BLAST | 118 | |
Transmembranei | 119 – 150 | HelicalBy similarityAdd BLAST | 32 | |
Topological domaini | 151 – 158 | ExtracellularCurated | 8 | |
Transmembranei | 159 – 179 | HelicalBy similarityAdd BLAST | 21 | |
Topological domaini | 180 – 183 | CytoplasmicCurated | 4 | |
Intramembranei | 184 – 195 | HelicalBy similarityAdd BLAST | 12 | |
Topological domaini | 196 – 208 | CytoplasmicCuratedAdd BLAST | 13 | |
Transmembranei | 209 – 228 | HelicalBy similarityAdd BLAST | 20 | |
Transmembranei | 229 – 246 | HelicalBy similarityAdd BLAST | 18 | |
Topological domaini | 247 – 268 | CytoplasmicCuratedAdd BLAST | 22 | |
Intramembranei | 269 – 290 | HelicalBy similarityAdd BLAST | 22 | |
Topological domaini | 291 – 301 | CytoplasmicCuratedAdd BLAST | 11 | |
Transmembranei | 302 – 321 | HelicalBy similarityAdd BLAST | 20 | |
Topological domaini | 322 – 347 | ExtracellularCuratedAdd BLAST | 26 | |
Transmembranei | 348 – 376 | HelicalBy similarityAdd BLAST | 29 | |
Topological domaini | 377 – 390 | CytoplasmicCuratedAdd BLAST | 14 | |
Transmembranei | 391 – 408 | HelicalBy similarityAdd BLAST | 18 | |
Topological domaini | 409 – 414 | ExtracellularCurated | 6 | |
Intramembranei | 415 – 426 | HelicalBy similarityAdd BLAST | 12 | |
Topological domaini | 427 – 457 | ExtracellularCuratedAdd BLAST | 31 | |
Transmembranei | 458 – 478 | HelicalBy similarityAdd BLAST | 21 | |
Transmembranei | 479 – 498 | HelicalBy similarityAdd BLAST | 20 | |
Topological domaini | 499 – 521 | ExtracellularCuratedAdd BLAST | 23 | |
Intramembranei | 522 – 554 | HelicalBy similarityAdd BLAST | 33 | |
Topological domaini | 555 – 557 | ExtracellularCurated | 3 | |
Transmembranei | 558 – 578 | HelicalBy similarityAdd BLAST | 21 | |
Topological domaini | 579 – 994 | CytoplasmicCuratedAdd BLAST | 416 |
Keywords - Cellular componenti
Cell membrane, MembranePathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 553 | I → T: Causes myotonia. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000094430 | 1 – 994 | Chloride channel protein 1Add BLAST | 994 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 892 | PhosphoserineCombined sources | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
PaxDbi | Q64347 |
PRIDEi | Q64347 |
PTM databases
iPTMneti | Q64347 |
PhosphoSitePlusi | Q64347 |
Expressioni
Tissue specificityi
Predominantly expressed in skeletal muscles.1 Publication
Gene expression databases
Bgeei | ENSMUSG00000029862, Expressed in skeletal muscle tissue and 111 other tissues |
ExpressionAtlasi | Q64347, baseline and differential |
Interactioni
Subunit structurei
Homodimer.
By similarityGO - Molecular functioni
- protein homodimerization activity Source: UniProtKB
Protein-protein interaction databases
STRINGi | 10090.ENSMUSP00000031894 |
Miscellaneous databases
RNActi | Q64347, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 609 – 668 | CBS 1PROSITE-ProRule annotationAdd BLAST | 60 | |
Domaini | 827 – 882 | CBS 2PROSITE-ProRule annotationAdd BLAST | 56 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 188 – 192 | Selectivity filter part_1By similarity | 5 | |
Motifi | 230 – 234 | Selectivity filter part_2By similarity | 5 | |
Motifi | 482 – 486 | Selectivity filter part_3By similarity | 5 |
Sequence similaritiesi
Belongs to the chloride channel (TC 2.A.49) family. ClC-1/CLCN1 subfamily. [View classification]Curated
Keywords - Domaini
CBS domain, Repeat, Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG0476, Eukaryota |
GeneTreei | ENSGT00940000157383 |
HOGENOMi | CLU_006904_0_1_1 |
InParanoidi | Q64347 |
OMAi | TYAQMQP |
OrthoDBi | 1131873at2759 |
PhylomeDBi | Q64347 |
TreeFami | TF352264 |
Family and domain databases
Gene3Di | 1.10.3080.10, 1 hit |
InterProi | View protein in InterPro IPR000644, CBS_dom IPR014743, Cl-channel_core IPR001807, Cl-channel_volt-gated IPR002243, Cl_channel-1 |
Pfami | View protein in Pfam PF00654, Voltage_CLC, 1 hit |
PRINTSi | PR00762, CLCHANNEL PR01112, CLCHANNEL1 |
SUPFAMi | SSF81340, SSF81340, 1 hit |
PROSITEi | View protein in PROSITE PS51371, CBS, 2 hits |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 8 potential isoforms that are computationally mapped.Show allAlign All
Q64347-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MERSQSQRHG GEQSWWGSAP QYQYMPFEHC TSYGLPSENG GLQHRPRKDM
60 70 80 90 100
GPRHNAHPTQ IYGHQKEQYS YKAQDGGMPK KMGSSSTMDS LDEDHYSKCQ
110 120 130 140 150
DCVHRLGRVL RRKLGEDWIF LVLLGLLMAL VSWCMDYVSA KSLQAYKWTY
160 170 180 190 200
AQMKPSLPLQ YLAWVTFPLI LILFSALFCQ LISPQAVGSG IPEMKTILRG
210 220 230 240 250
VVLKEYLTLK AFVAKVVALT AGLGSGIPVG KEGPFVHIAS ICAAVLSKFM
260 270 280 290 300
SMFSGVYEQP YYYTDILTVG CAVGVGCCFG TPLGGVLFSI EVTSTYFAVR
310 320 330 340 350
NYWRGFFAAT FSAFVFRVLA VWNKDAVTIT ALFRTNFRMD FPFDLKELPA
360 370 380 390 400
FAVIGICCGF LGAVFVYLHR QVMLGVRKHK CLSQFLAKHR LLYPGIVTFV
410 420 430 440 450
IASLTFPPGM GQFMAGELMP REAISTLFDN NTWVKHIGDP QSLGQSAVWL
460 470 480 490 500
HPQVNVIIII LLFFVMKFWM SIVATTMPIP CGGFMPVFVL GAAFGRLVGE
510 520 530 540 550
IMAMLFPEGI LFDDIIYKIL PGGYAVIGAA ALTGAVSHTV STAVICFELT
560 570 580 590 600
GQIAHILPMM VAVILANMVA QSLQPSLYDS IIQVKKLPYL PDLGWNQLSK
610 620 630 640 650
FTIFVEDIMV RDVKFVSASC TYGELRNLLQ ATTVKTLPLV DSKDSMILLG
660 670 680 690 700
SVERSELQSL LQRHLCAERR LKAAQDMARK LSELPYNGKA QLAGDWHPGG
710 720 730 740 750
RPESFAFVDE DEDEDLSRKM ELPLTPAPPP PSPPPPPSQF PIAPSNPEEP
760 770 780 790 800
NGPLPSHKQP PEASDSADQR SSTFQRLLHC LLGKAHSKKK KITQDSTDLV
810 820 830 840 850
DNMSPEEIEA WEREQLSQPV CFDCCCIDQS PFQLVEQTTL HKTHTLFSLL
860 870 880 890 900
GLHLAYVTSM GKLRGVLALE ELQKAIEGHT KSGVQLRPPL ASFRNTTSIR
910 920 930 940 950
KTPGGPPPPA EGWNVPEDGD GAPGREVMVP TMPETPVPPP SPEAPSCLAP
960 970 980 990
ARAEGELEEL EMVGSLEPEE ELADILHGPS LRSTDEEDED ELIL
Computationally mapped potential isoform sequencesi
There are 8 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketF6QI82 | F6QI82_MOUSE | Chloride channel protein | Clcn1 | 923 | Annotation score: | ||
F6QL23 | F6QL23_MOUSE | Chloride channel protein 1 | Clcn1 | 229 | Annotation score: | ||
F6X177 | F6X177_MOUSE | Chloride channel protein 1 | Clcn1 | 289 | Annotation score: | ||
F7A9J6 | F7A9J6_MOUSE | Chloride channel protein 1 | Clcn1 | 257 | Annotation score: | ||
F7C9S1 | F7C9S1_MOUSE | Chloride channel protein 1 | Clcn1 | 262 | Annotation score: | ||
E9Q2N3 | E9Q2N3_MOUSE | Chloride channel protein 1 | Clcn1 | 258 | Annotation score: | ||
A0A5F8MPY7 | A0A5F8MPY7_MOUSE | Chloride channel protein 1 | Clcn1 | 326 | Annotation score: | ||
F6VSA8 | F6VSA8_MOUSE | Chloride channel protein 1 | Clcn1 | 104 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 65 | Q → H in CAB08359 (PubMed:9321463).Curated | 1 | |
Sequence conflicti | 72 | K → Q in CAB08359 (PubMed:9321463).Curated | 1 | |
Sequence conflicti | 76 – 78 | GGM → RGI in CAB08359 (PubMed:9321463).Curated | 3 | |
Sequence conflicti | 82 – 83 | MG → TD in CAB08359 (PubMed:9321463).Curated | 2 | |
Sequence conflicti | 88 | M → V in CAB08359 (PubMed:9321463).Curated | 1 | |
Sequence conflicti | 243 | A → T in CAB08359 (PubMed:9321463).Curated | 1 | |
Sequence conflicti | 483 – 484 | GF → VN in CAB08359 (PubMed:9321463).Curated | 2 | |
Sequence conflicti | 585 | K → E in CAB08359 (PubMed:9321463).Curated | 1 | |
Sequence conflicti | 620 | C → S in CAB08359 (PubMed:9321463).Curated | 1 | |
Sequence conflicti | 631 | A → T in CAB08359 (PubMed:9321463).Curated | 1 | |
Sequence conflicti | 746 | N → H no nucleotide entry (PubMed:8119941).Curated | 1 | |
Sequence conflicti | 864 | R → T in CAB08359 (PubMed:9321463).Curated | 1 | |
Sequence conflicti | 877 | E → K in CAB08359 (PubMed:9321463).Curated | 1 | |
Sequence conflicti | 912 | G → S in CAB08359 (PubMed:9321463).Curated | 1 | |
Sequence conflicti | 918 – 919 | DG → GE in CAB08359 (PubMed:9321463).Curated | 2 | |
Sequence conflicti | 924 | G → E in CAB08359 (PubMed:9321463).Curated | 1 | |
Sequence conflicti | 944 | A → V no nucleotide entry (PubMed:8119941).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Z95127 , Z95128, Z95129, Z95130, Z95131, Z95132, Z95133, Z95134, Z95135, Z95136, Z95137, Z95138, Z95139, Z95140, Z95141, Z95142, Z95143, Z95144, Z95145, Z95146, Z95147, Z95148, Z95149 Genomic DNA Translation: CAB08359.1 AK036735 mRNA Translation: BAC29557.1 CH466533 Genomic DNA Translation: EDL13488.1 BC114336 mRNA Translation: AAI14337.1 X62895 mRNA Translation: CAA44684.1 X62896 mRNA Translation: CAA44685.1 X62897 mRNA Translation: CAA44686.1 |
CCDSi | CCDS39471.1 |
PIRi | I48773 |
RefSeqi | NP_038519.1, NM_013491.2 |
Genome annotation databases
Ensembli | ENSMUST00000031894; ENSMUSP00000031894; ENSMUSG00000029862 |
GeneIDi | 12723 |
KEGGi | mmu:12723 |
UCSCi | uc009bqs.1, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Z95127 , Z95128, Z95129, Z95130, Z95131, Z95132, Z95133, Z95134, Z95135, Z95136, Z95137, Z95138, Z95139, Z95140, Z95141, Z95142, Z95143, Z95144, Z95145, Z95146, Z95147, Z95148, Z95149 Genomic DNA Translation: CAB08359.1 AK036735 mRNA Translation: BAC29557.1 CH466533 Genomic DNA Translation: EDL13488.1 BC114336 mRNA Translation: AAI14337.1 X62895 mRNA Translation: CAA44684.1 X62896 mRNA Translation: CAA44685.1 X62897 mRNA Translation: CAA44686.1 |
CCDSi | CCDS39471.1 |
PIRi | I48773 |
RefSeqi | NP_038519.1, NM_013491.2 |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
STRINGi | 10090.ENSMUSP00000031894 |
PTM databases
iPTMneti | Q64347 |
PhosphoSitePlusi | Q64347 |
Proteomic databases
PaxDbi | Q64347 |
PRIDEi | Q64347 |
Protocols and materials databases
Antibodypediai | 32627, 133 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000031894; ENSMUSP00000031894; ENSMUSG00000029862 |
GeneIDi | 12723 |
KEGGi | mmu:12723 |
UCSCi | uc009bqs.1, mouse |
Organism-specific databases
CTDi | 1180 |
MGIi | MGI:88417, Clcn1 |
Phylogenomic databases
eggNOGi | KOG0476, Eukaryota |
GeneTreei | ENSGT00940000157383 |
HOGENOMi | CLU_006904_0_1_1 |
InParanoidi | Q64347 |
OMAi | TYAQMQP |
OrthoDBi | 1131873at2759 |
PhylomeDBi | Q64347 |
TreeFami | TF352264 |
Enzyme and pathway databases
Reactomei | R-MMU-2672351, Stimuli-sensing channels |
Miscellaneous databases
BioGRID-ORCSi | 12723, 4 hits in 17 CRISPR screens |
PROi | PR:Q64347 |
RNActi | Q64347, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000029862, Expressed in skeletal muscle tissue and 111 other tissues |
ExpressionAtlasi | Q64347, baseline and differential |
Family and domain databases
Gene3Di | 1.10.3080.10, 1 hit |
InterProi | View protein in InterPro IPR000644, CBS_dom IPR014743, Cl-channel_core IPR001807, Cl-channel_volt-gated IPR002243, Cl_channel-1 |
Pfami | View protein in Pfam PF00654, Voltage_CLC, 1 hit |
PRINTSi | PR00762, CLCHANNEL PR01112, CLCHANNEL1 |
SUPFAMi | SSF81340, SSF81340, 1 hit |
PROSITEi | View protein in PROSITE PS51371, CBS, 2 hits |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | CLCN1_MOUSE | |
Accessioni | Q64347Primary (citable) accession number: Q64347 Secondary accession number(s): Q8BZ41, Q9QVY5 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 21, 2001 |
Last sequence update: | May 18, 2010 | |
Last modified: | December 2, 2020 | |
This is version 159 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families