UniProtKB - Q64346 (DUS6_RAT)
Protein
Dual specificity protein phosphatase 6
Gene
Dusp6
Organism
Rattus norvegicus (Rat)
Status
Functioni
Inactivates MAP kinases. Has a specificity for the ERK family (PubMed:8626780). Implicated in muscle and neuronal differentiation (PubMed:8631996). Plays an important role in alleviating chronic postoperative pain. Necessary for the normal dephosphorylation of the long-lasting phosphorylated forms of spinal MAPK1/3 and MAP kinase p38 induced by peripheral surgery, which drives the resolution of acute postoperative allodynia (By similarity). Also important for dephosphorylation of MAPK1/3 in local wound tissue, which further contributes to resolution of acute pain (By similarity).By similarity2 Publications
Catalytic activityi
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 293 | Phosphocysteine intermediatePROSITE-ProRule annotation | 1 |
GO - Molecular functioni
- MAP kinase tyrosine/serine/threonine phosphatase activity Source: RGD
- mitogen-activated protein kinase binding Source: GO_Central
- phosphoprotein phosphatase activity Source: RGD
- protein tyrosine/serine/threonine phosphatase activity Source: RGD
- protein tyrosine/threonine phosphatase activity Source: GO_Central
- protein tyrosine phosphatase activity Source: UniProtKB-EC
GO - Biological processi
- cell differentiation Source: RGD
- inactivation of MAPK activity Source: RGD
- negative regulation of ERK1 and ERK2 cascade Source: RGD
- negative regulation of protein phosphorylation Source: RGD
- peptidyl-threonine dephosphorylation Source: GO_Central
- peptidyl-tyrosine dephosphorylation Source: RGD
- positive regulation of apoptotic process Source: RGD
- positive regulation of cell death Source: RGD
- protein dephosphorylation Source: RGD
- regulation of heart growth Source: RGD
- response to drug Source: RGD
- response to growth factor Source: RGD
- response to nitrosative stress Source: RGD
- response to organic cyclic compound Source: RGD
- response to organic substance Source: RGD
Keywordsi
Molecular function | Hydrolase, Protein phosphatase |
Enzyme and pathway databases
Reactomei | R-RNO-112409, RAF-independent MAPK1/3 activation R-RNO-202670, ERKs are inactivated R-RNO-5675221, Negative regulation of MAPK pathway |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:Dusp6 Synonyms:Mkp3 |
Organismi | Rattus norvegicus (Rat) |
Taxonomic identifieri | 10116 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Rattus |
Proteomesi |
|
Organism-specific databases
RGDi | 70978, Dusp6 |
Subcellular locationi
Other locations
- Cytoplasm 1 Publication
Cytosol
- cytosol Source: RGD
Nucleus
- nucleoplasm Source: Ensembl
Other locations
- cytoplasm Source: RGD
Keywords - Cellular componenti
CytoplasmPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000094806 | 1 – 381 | Dual specificity protein phosphatase 6Add BLAST | 381 |
Proteomic databases
PaxDbi | Q64346 |
PTM databases
iPTMneti | Q64346 |
PhosphoSitePlusi | Q64346 |
Expressioni
Tissue specificityi
Expressed in lung, heart, brain, and kidney, but not significantly in skeletal muscle or testis.1 Publication
Gene expression databases
Bgeei | ENSRNOG00000023896, Expressed in lung and 21 other tissues |
Genevisiblei | Q64346, RN |
Interactioni
GO - Molecular functioni
- mitogen-activated protein kinase binding Source: GO_Central
Protein-protein interaction databases
DIPi | DIP-61143N |
IntActi | Q64346, 1 interactor |
STRINGi | 10116.ENSRNOP00000032969 |
Chemistry databases
BindingDBi | Q64346 |
Structurei
3D structure databases
SMRi | Q64346 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | Q64346 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 30 – 148 | RhodanesePROSITE-ProRule annotationAdd BLAST | 119 | |
Domaini | 206 – 349 | Tyrosine-protein phosphatasePROSITE-ProRule annotationAdd BLAST | 144 |
Sequence similaritiesi
Belongs to the protein-tyrosine phosphatase family. Non-receptor class dual specificity subfamily.Curated
Phylogenomic databases
eggNOGi | KOG1717, Eukaryota |
GeneTreei | ENSGT00940000158342 |
HOGENOMi | CLU_027074_0_0_1 |
InParanoidi | Q64346 |
OMAi | SPCDNRM |
OrthoDBi | 911920at2759 |
PhylomeDBi | Q64346 |
TreeFami | TF105122 |
Family and domain databases
Gene3Di | 3.40.250.10, 1 hit 3.90.190.10, 1 hit |
InterProi | View protein in InterPro IPR000340, Dual-sp_phosphatase_cat-dom IPR008343, MKP IPR029021, Prot-tyrosine_phosphatase-like IPR001763, Rhodanese-like_dom IPR036873, Rhodanese-like_dom_sf IPR000387, TYR_PHOSPHATASE_dom IPR020422, TYR_PHOSPHATASE_DUAL_dom |
Pfami | View protein in Pfam PF00782, DSPc, 1 hit PF00581, Rhodanese, 1 hit |
PIRSFi | PIRSF000939, MAPK_Ptase, 1 hit |
PRINTSi | PR01764, MAPKPHPHTASE |
SMARTi | View protein in SMART SM00195, DSPc, 1 hit SM00450, RHOD, 1 hit |
SUPFAMi | SSF52799, SSF52799, 1 hit SSF52821, SSF52821, 1 hit |
PROSITEi | View protein in PROSITE PS50206, RHODANESE_3, 1 hit PS50056, TYR_PHOSPHATASE_2, 1 hit PS50054, TYR_PHOSPHATASE_DUAL, 1 hit |
i Sequence
Sequence statusi: Complete.
Q64346-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MIDTLRPVPF ASEMAISKTV AWLNEQLELG NEQLLLMDCR PQELYESSHI
60 70 80 90 100
ESAINVAIPG IMLRRLQKGN LPVRALFTRC EDRDRFTRRC GTDTVVLYDE
110 120 130 140 150
NSSDWNENTG GESVLGLLLK KLKDEGCRAF YLEGGFSKFQ AEFALHCETN
160 170 180 190 200
LDGSCSSSSP PLPVLGLGGL RISSDSSSDI ESDLDRDPNS ATDSDGSPLS
210 220 230 240 250
NSQPSFPVEI LPFLYLGCAK DSTNLDVLEE FGIKYILNVT PNLPNLFENA
260 270 280 290 300
GEFKYKQIPI SDHWSQNLSQ FFPEAISFID EARGKNCGVL VHCLAGISRS
310 320 330 340 350
VTVTVAYLMQ KLNLSMNDAY DIVKMKKSNI SPNFNFMGQL LDFERTLGLS
360 370 380
SPCDNRVPAQ QLYFTAPSNQ NVYQVDSLQS T
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X94185 mRNA Translation: CAA63895.1 U42627 mRNA Translation: AAB06202.1 BC087003 mRNA Translation: AAH87003.1 |
RefSeqi | NP_446335.1, NM_053883.2 |
Genome annotation databases
Ensembli | ENSRNOT00000037844; ENSRNOP00000032969; ENSRNOG00000023896 |
GeneIDi | 116663 |
KEGGi | rno:116663 |
UCSCi | RGD:70978, rat |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X94185 mRNA Translation: CAA63895.1 U42627 mRNA Translation: AAB06202.1 BC087003 mRNA Translation: AAH87003.1 |
RefSeqi | NP_446335.1, NM_053883.2 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
2FYS | X-ray | 2.50 | C/D | 60-76 | [»] | |
SMRi | Q64346 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
DIPi | DIP-61143N |
IntActi | Q64346, 1 interactor |
STRINGi | 10116.ENSRNOP00000032969 |
Chemistry databases
BindingDBi | Q64346 |
ChEMBLi | CHEMBL5511 |
DrugCentrali | Q64346 |
PTM databases
iPTMneti | Q64346 |
PhosphoSitePlusi | Q64346 |
Proteomic databases
PaxDbi | Q64346 |
Genome annotation databases
Ensembli | ENSRNOT00000037844; ENSRNOP00000032969; ENSRNOG00000023896 |
GeneIDi | 116663 |
KEGGi | rno:116663 |
UCSCi | RGD:70978, rat |
Organism-specific databases
CTDi | 1848 |
RGDi | 70978, Dusp6 |
Phylogenomic databases
eggNOGi | KOG1717, Eukaryota |
GeneTreei | ENSGT00940000158342 |
HOGENOMi | CLU_027074_0_0_1 |
InParanoidi | Q64346 |
OMAi | SPCDNRM |
OrthoDBi | 911920at2759 |
PhylomeDBi | Q64346 |
TreeFami | TF105122 |
Enzyme and pathway databases
Reactomei | R-RNO-112409, RAF-independent MAPK1/3 activation R-RNO-202670, ERKs are inactivated R-RNO-5675221, Negative regulation of MAPK pathway |
Miscellaneous databases
EvolutionaryTracei | Q64346 |
PROi | PR:Q64346 |
Gene expression databases
Bgeei | ENSRNOG00000023896, Expressed in lung and 21 other tissues |
Genevisiblei | Q64346, RN |
Family and domain databases
Gene3Di | 3.40.250.10, 1 hit 3.90.190.10, 1 hit |
InterProi | View protein in InterPro IPR000340, Dual-sp_phosphatase_cat-dom IPR008343, MKP IPR029021, Prot-tyrosine_phosphatase-like IPR001763, Rhodanese-like_dom IPR036873, Rhodanese-like_dom_sf IPR000387, TYR_PHOSPHATASE_dom IPR020422, TYR_PHOSPHATASE_DUAL_dom |
Pfami | View protein in Pfam PF00782, DSPc, 1 hit PF00581, Rhodanese, 1 hit |
PIRSFi | PIRSF000939, MAPK_Ptase, 1 hit |
PRINTSi | PR01764, MAPKPHPHTASE |
SMARTi | View protein in SMART SM00195, DSPc, 1 hit SM00450, RHOD, 1 hit |
SUPFAMi | SSF52799, SSF52799, 1 hit SSF52821, SSF52821, 1 hit |
PROSITEi | View protein in PROSITE PS50206, RHODANESE_3, 1 hit PS50056, TYR_PHOSPHATASE_2, 1 hit PS50054, TYR_PHOSPHATASE_DUAL, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | DUS6_RAT | |
Accessioni | Q64346Primary (citable) accession number: Q64346 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 1, 1997 |
Last sequence update: | November 1, 1996 | |
Last modified: | December 2, 2020 | |
This is version 148 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families