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UniProtKB - Q64329 (KLRA3_MOUSE)
Protein
Killer cell lectin-like receptor 3
Gene
Klra3
Organism
Mus musculus (Mouse)
Status
Functioni
Receptor on natural killer (NK) cells for class I MHC.
1 PublicationGO - Molecular functioni
- carbohydrate binding Source: UniProtKB-KW
GO - Biological processi
- cell adhesion Source: UniProtKB-KW
Keywordsi
Molecular function | Receptor |
Biological process | Cell adhesion |
Ligand | Lectin |
Names & Taxonomyi
Protein namesi | Recommended name: Killer cell lectin-like receptor 3Alternative name(s): 5E6 Lymphocyte antigen 49c Short name: Ly-49c Nk2.1 T-cell surface glycoprotein Ly-49C |
Gene namesi | Name:Klra3 Synonyms:Ly-49c, Ly49C |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:101905, Klra3 |
VEuPathDBi | HostDB:ENSMUSG00000067591 |
Subcellular locationi
Other locations
Plasma Membrane
- plasma membrane Source: MGI
Other locations
- integral component of membrane Source: UniProtKB-KW
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 48 | CytoplasmicSequence analysisAdd BLAST | 48 | |
Transmembranei | 49 – 69 | Helical; Signal-anchor for type II membrane proteinSequence analysisAdd BLAST | 21 | |
Topological domaini | 70 – 266 | ExtracellularSequence analysisAdd BLAST | 197 |
Keywords - Cellular componenti
MembranePathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 121 | R → G: Increases stability and improves binding to MHC I class ligand; when associated with G-175; G-197 and K-227. 1 Publication | 1 | |
Mutagenesisi | 160 | N → D: Greatly reduces MHC class I peptide tetramer binding; when associated with L-162. 1 Publication | 1 | |
Mutagenesisi | 161 | K → R: No effect on MHC class I peptide tetramer binding. 1 Publication | 1 | |
Mutagenesisi | 162 | T → K: Greatly reduces MHC class I peptide tetramer binding; when associated with D-160. 1 Publication | 1 | |
Mutagenesisi | 169 | A → Q: No effect on MHC class I peptide tetramer binding; when associated with T-170. 1 Publication | 1 | |
Mutagenesisi | 170 | N → T: No effect on MHC class I peptide tetramer binding; when associated with Q-169. 1 Publication | 1 | |
Mutagenesisi | 173 | H → S: No effect on MHC class I peptide tetramer binding; when associated with S-174. 1 Publication | 1 | |
Mutagenesisi | 174 | Y → S: No effect on MHC class I binding; when associated with S-173. 1 Publication | 1 | |
Mutagenesisi | 175 | S → G: Increases stability. Increases stability and improves binding to MHC I class ligand; when associated with G-121; G-197 and K-227. 1 Publication | 1 | |
Mutagenesisi | 195 | I → P: Greatly reduces MHC class I peptide tetramer binding; when associated with S-196. 1 Publication | 1 | |
Mutagenesisi | 196 | P → S: Greatly reduces MHC class I peptide tetramer binding; when associated with P-195. 1 Publication | 1 | |
Mutagenesisi | 197 | E → G: Increases stability and improves binding to MHC I class ligand; when associated with G-121; G-175 and K-227. 1 Publication | 1 | |
Mutagenesisi | 225 | K → N: Greatly reduces MHC class I peptide tetramer binding; when associated with T-226. 1 Publication | 1 | |
Mutagenesisi | 226 | I → T: Greatly reduces MHC class I peptide tetramer binding; when associated with N-225. 1 Publication | 1 | |
Mutagenesisi | 227 | R → K: Increases stability and improves binding to MHC I class ligand; when associated with G-121; G-175 and G-197. 1 Publication | 1 | |
Mutagenesisi | 234 | R → G: No effect on MHC class I peptide tetramer binding. 1 Publication | 1 | |
Mutagenesisi | 248 | D → N: No effect on MHC class I peptide tetramer binding; when associated with D-250. 1 Publication | 1 | |
Mutagenesisi | 250 | N → D: No effect on MHC class I peptide tetramer binding; when associated with N-248. 1 Publication | 1 | |
Mutagenesisi | 251 | I → Q: No effect on MHC class I peptide tetramer binding. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000046681 | 1 – 266 | Killer cell lectin-like receptor 3Add BLAST | 266 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Glycosylationi | 79 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 87 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 104 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 113 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 149 ↔ 154 | |||
Glycosylationi | 160 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 167 ↔ 255 | |||
Disulfide bondi | 171 ↔ 257 | |||
Disulfide bondi | 236 ↔ 249 |
Keywords - PTMi
Disulfide bond, GlycoproteinProteomic databases
EPDi | Q64329 |
PaxDbi | Q64329 |
PRIDEi | Q64329 |
PTM databases
GlyGeni | Q64329, 5 sites |
Expressioni
Gene expression databases
Bgeei | ENSMUSG00000067591, Expressed in thymus and 26 other tissues |
Genevisiblei | Q64329, MM |
Interactioni
Subunit structurei
Homodimer; disulfide-linked.
2 PublicationsProtein-protein interaction databases
BioGRIDi | 200995, 1 interactor |
STRINGi | 10090.ENSMUSP00000107629 |
Miscellaneous databases
RNActi | Q64329, protein |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | Q64329 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | Q64329 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 150 – 258 | C-type lectinPROSITE-ProRule annotationAdd BLAST | 109 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 147 – 151 | Involved in dimerization | 5 | |
Regioni | 160 – 162 | Implicated in MHC class I binding | 3 | |
Regioni | 195 – 196 | Implicated in MHC class I binding | 2 | |
Regioni | 207 – 208 | Implicated in MHC class I binding | 2 | |
Regioni | 224 – 233 | Implicated in MHC class I binding | 10 | |
Regioni | 240 – 245 | Implicated in MHC class I binding | 6 |
Keywords - Domaini
Signal-anchor, Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG4297, Eukaryota |
GeneTreei | ENSGT00390000008117 |
HOGENOMi | CLU_049894_1_0_1 |
InParanoidi | Q64329 |
OMAi | KSSECDA |
OrthoDBi | 945977at2759 |
PhylomeDBi | Q64329 |
TreeFami | TF336674 |
Family and domain databases
CDDi | cd03593, CLECT_NK_receptors_like, 1 hit |
Gene3Di | 3.10.100.10, 1 hit |
InterProi | View protein in InterPro IPR001304, C-type_lectin-like IPR016186, C-type_lectin-like/link_sf IPR016187, CTDL_fold IPR013600, Ly49_N IPR033992, NKR-like_CTLD |
Pfami | View protein in Pfam PF00059, Lectin_C, 1 hit PF08391, Ly49, 1 hit |
SMARTi | View protein in SMART SM00034, CLECT, 1 hit |
SUPFAMi | SSF56436, SSF56436, 1 hit |
PROSITEi | View protein in PROSITE PS50041, C_TYPE_LECTIN_2, 1 hit |
i Sequence
Sequence statusi: Complete.
Q64329-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSEPEVTYST VRLHKSSGLQ KLVRHEETQG PREVGNRKCS APWQLIVKAL
60 70 80 90 100
GILCFLLLVT VAVLAVKIFQ YNQHKQEINE TLNHHHNCSN MQRAFNLKEE
110 120 130 140 150
MLTNKSIDCR PSNETLEYIK REQDRWDSKT KTVLDSSRDT GRGVKYWFCY
160 170 180 190 200
STKCYYFIMN KTTWSGCKAN CQHYSVPILK IEDEDELKFL QRHVIPENYW
210 220 230 240 250
IGLSYDKKKK EWAWIDNGPS KLDMKIRKMN FKSRGCVFLS KARIEDIDCN
260
IPYYCICGKK LDKFPD
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 4 | P → L in AAH99880 (PubMed:15489334).Curated | 1 | |
Sequence conflicti | 4 | P → L in AAH99879 (PubMed:15489334).Curated | 1 | |
Sequence conflicti | 4 | P → L in AAH99878 (PubMed:15489334).Curated | 1 | |
Sequence conflicti | 4 | P → L in AAH99867 (PubMed:15489334).Curated | 1 | |
Sequence conflicti | 162 | T → M in BAE35215 (PubMed:16141072).Curated | 1 |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural varianti | 2 | S → N in strain: C57BL/6 and C57BL/6 X BALB/c. | 1 | |
Natural varianti | 22 | L → Q in strain: A/Sn, BALB/c, C57BL/6, C57BL/6 X BALB/c, C57BL/6 X CBA, CB-17/SCID and NZB. | 1 | |
Natural varianti | 34 | V → A in strain: C57BL/6, C57BL/6 X BALB/c and NZB. | 1 | |
Natural varianti | 41 – 42 | AP → VS in strain: C57BL/6 X BALB/c, NZB and C57BL/6. | 2 | |
Natural varianti | 60 | T → I in strain: C57BL/6, C57BL/6 X BALB/c and NZB. | 1 | |
Natural varianti | 65 – 66 | AV → TI in strain: C57BL/6, C57BL/6 X BALB/c and NZB. | 2 | |
Natural varianti | 72 | N → S in strain: C57BL/6, C57BL/6 X BALB/c and NZB. | 1 | |
Natural varianti | 85 | H → Y in strain: C57BL/6, C57BL/6 X BALB/c and NZB. | 1 | |
Natural varianti | 93 – 94 | RA → SD in strain: A/Sn, BALB/c, C57BL/6, C57BL/6 X BALB/c, C57BL/6 X CBA, CB-17/SCID and NZB. | 2 | |
Natural varianti | 115 | T → L in strain: C57BL/6, C57BL/6 X BALB/c and NZB; requires 2 nucleotide substitutions. | 1 | |
Natural varianti | 117 | E → D in strain: C57BL/6 and C57BL/6 X BALB/c. | 1 | |
Natural varianti | 127 | D → N in strain: C57BL/6 and C57BL/6 X BALB/c. | 1 | |
Natural varianti | 129 | K → E in strain: C57BL/6, C57BL/6 X BALB/c and NZB. | 1 | |
Natural varianti | 131 | K → N in strain: NZB. | 1 | |
Natural varianti | 133 | V → I in strain: NZB. | 1 | |
Natural varianti | 146 | Y → H in strain: C57BL/6 and C57BL/6 X BALB/c. | 1 | |
Natural varianti | 151 | S → G in strain: C57BL/6, C57BL/6 X BALB/c and NZB. | 1 | |
Natural varianti | 174 | Y → F in strain: A/Sn, BALB/c, C57BL/6, C57BL/6 X BALB/c, C57BL/6 X CBA, CB-17/SCID and NZB. | 1 | |
Natural varianti | 179 | L → V in strain: C57BL/6, C57BL/6 X BALB/c and NZB. | 1 | |
Natural varianti | 189 | F → S in strain: NZB. | 1 | |
Natural varianti | 198 | N → S in strain: C57BL/6, C57BL/6 X BALB/c and NZB. | 1 | |
Natural varianti | 219 | P → Q in strain: C57BL/6, C57BL/6 X BALB/c and NZB. | 1 | |
Natural varianti | 226 | I → T in strain: C57BL/6, C57BL/6 X BALB/c and NZB. | 1 | |
Natural varianti | 232 | K → T in strain: NZB. | 1 | |
Natural varianti | 247 | I → T in strain: C57BL/6, C57BL/6 X BALB/c and NZB. | 1 | |
Natural varianti | 251 | I → T in strain: NZB. | 1 | |
Natural varianti | 260 | K → R in strain: NZB. | 1 |
Sequence databases
Genome annotation databases
Ensembli | ENSMUST00000088017; ENSMUSP00000085333; ENSMUSG00000067591 ENSMUST00000111998; ENSMUSP00000107629; ENSMUSG00000067591 |
GeneIDi | 16634 16640 |
KEGGi | mmu:16634 mmu:16640 |
UCSCi | uc009eik.2, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1P1Z | X-ray | 3.26 | D | 143-262 | [»] | |
1P4L | X-ray | 2.90 | D | 142-263 | [»] | |
3C8J | X-ray | 2.60 | A/B/C/D | 67-266 | [»] | |
3C8K | X-ray | 2.90 | D | 142-266 | [»] | |
5J6G | X-ray | 3.30 | G/H | 136-266 | [»] | |
SMRi | Q64329 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 200995, 1 interactor |
STRINGi | 10090.ENSMUSP00000107629 |
PTM databases
GlyGeni | Q64329, 5 sites |
Proteomic databases
EPDi | Q64329 |
PaxDbi | Q64329 |
PRIDEi | Q64329 |
Protocols and materials databases
DNASUi | 16640 |
Genome annotation databases
Ensembli | ENSMUST00000088017; ENSMUSP00000085333; ENSMUSG00000067591 ENSMUST00000111998; ENSMUSP00000107629; ENSMUSG00000067591 |
GeneIDi | 16634 16640 |
KEGGi | mmu:16634 mmu:16640 |
UCSCi | uc009eik.2, mouse |
Organism-specific databases
CTDi | 16634 16640 |
MGIi | MGI:101905, Klra3 |
VEuPathDBi | HostDB:ENSMUSG00000067591 |
Phylogenomic databases
eggNOGi | KOG4297, Eukaryota |
GeneTreei | ENSGT00390000008117 |
HOGENOMi | CLU_049894_1_0_1 |
InParanoidi | Q64329 |
OMAi | KSSECDA |
OrthoDBi | 945977at2759 |
PhylomeDBi | Q64329 |
TreeFami | TF336674 |
Miscellaneous databases
BioGRID-ORCSi | 16634, 0 hits in 32 CRISPR screens 16640, 0 hits in 31 CRISPR screens |
ChiTaRSi | Klra3, mouse |
EvolutionaryTracei | Q64329 |
PROi | PR:Q64329 |
RNActi | Q64329, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000067591, Expressed in thymus and 26 other tissues |
Genevisiblei | Q64329, MM |
Family and domain databases
CDDi | cd03593, CLECT_NK_receptors_like, 1 hit |
Gene3Di | 3.10.100.10, 1 hit |
InterProi | View protein in InterPro IPR001304, C-type_lectin-like IPR016186, C-type_lectin-like/link_sf IPR016187, CTDL_fold IPR013600, Ly49_N IPR033992, NKR-like_CTLD |
Pfami | View protein in Pfam PF00059, Lectin_C, 1 hit PF08391, Ly49, 1 hit |
SMARTi | View protein in SMART SM00034, CLECT, 1 hit |
SUPFAMi | SSF56436, SSF56436, 1 hit |
PROSITEi | View protein in PROSITE PS50041, C_TYPE_LECTIN_2, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | KLRA3_MOUSE | |
Accessioni | Q64329Primary (citable) accession number: Q64329 Secondary accession number(s): Q3TWQ1 Q64257 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 15, 1998 |
Last sequence update: | October 31, 2012 | |
Last modified: | February 23, 2022 | |
This is version 167 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references