UniProtKB - Q64291 (K1C12_MOUSE)
Protein
Keratin, type I cytoskeletal 12
Gene
Krt12
Organism
Mus musculus (Mouse)
Status
Functioni
Involved in corneal epithelium organization, integrity and corneal keratin expression.1 Publication
Miscellaneous
There are two types of cytoskeletal and microfibrillar keratin: I (acidic; 40-55 kDa) and II (neutral to basic; 56-70 kDa).
GO - Molecular functioni
- structural molecule activity Source: InterPro
GO - Biological processi
- cornea development in camera-type eye Source: UniProtKB
- epithelium development Source: MGI
- morphogenesis of an epithelium Source: UniProtKB
Enzyme and pathway databases
Reactomei | R-MMU-6805567, Keratinization R-MMU-6809371, Formation of the cornified envelope |
Names & Taxonomyi
Protein namesi | Recommended name: Keratin, type I cytoskeletal 12Alternative name(s): Cytokeratin-12 Short name: CK-12 Keratin-12 Short name: K12 |
Gene namesi | Name:Krt12 Synonyms:Krt1-12, Krt1.12 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:96687, Krt12 |
Subcellular locationi
Cytoskeleton
- intermediate filament Source: UniProtKB-KW
Keywords - Cellular componenti
Intermediate filament, KeratinPathology & Biotechi
Disruption phenotypei
Knockout mice are fertile and morphologically normal with no defects in the skin or hair (PubMed:8977471). Corneal epithelia show histological abnormalities and can be easily detached from the corneal surface (PubMed:8977471). Reduced number of cell layers in the corneal epithelium, with superficial epithelial cells showing reduced adherence to underlying cell layers (PubMed:8977471). Keratin intermediate filaments aggregate into dense bundles in the corneal epithelium, with a reduced number of keratin filaments in basal and suprabasal epithelial cells, and an absence of keratin filaments in superficial epithelial cells (PubMed:8977471).1 Publication
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000063645 | 1 – 487 | Keratin, type I cytoskeletal 12Add BLAST | 487 |
Proteomic databases
PaxDbi | Q64291 |
PeptideAtlasi | Q64291 |
PRIDEi | Q64291 |
PTM databases
PhosphoSitePlusi | Q64291 |
Expressioni
Tissue specificityi
Expressed in the corneal epithelium (at protein level) (PubMed:7508359, PubMed:8977471, PubMed:26758872). Also expressed in the suprabasal limbal epithelium of the cornea (at protein level) (PubMed:7508359).3 Publications
Developmental stagei
Expression commences in corneal peridermal epithelium in the embryo at 15.5 dpc (PubMed:16431949). Between 15.5 dpc and postnatal day 4 (P4), expression is restricted to the suprabasal and/or superficial cells when the corneal epithelium begins to stratify (PubMed:7508359, PubMed:16431949). At P30 expression is sporadically detected in the basal corneal epithelium and the number of positive basal cells increases as the mice grow older (PubMed:16431949).2 Publications
Inductioni
Expression is retarded by epidermal growth factor (EGF).1 Publication
Interactioni
Subunit structurei
Heterotetramer of two type I and two type II keratins. Keratin-3 associates with keratin-12.
Protein-protein interaction databases
STRINGi | 10090.ENSMUSP00000017741 |
Miscellaneous databases
RNActi | Q64291, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 119 – 433 | IF rodPROSITE-ProRule annotationAdd BLAST | 315 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 118 | HeadAdd BLAST | 118 | |
Regioni | 119 – 154 | Coil 1AAdd BLAST | 36 | |
Regioni | 158 – 175 | Linker 1Add BLAST | 18 | |
Regioni | 176 – 267 | Coil 1BAdd BLAST | 92 | |
Regioni | 268 – 290 | Linker 12Add BLAST | 23 | |
Regioni | 291 – 428 | Coil 2Add BLAST | 138 | |
Regioni | 429 – 487 | TailAdd BLAST | 59 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 19 – 117 | Gly-richAdd BLAST | 99 |
Sequence similaritiesi
Belongs to the intermediate filament family.PROSITE-ProRule annotation
Keywords - Domaini
Coiled coilPhylogenomic databases
eggNOGi | ENOG502QTM6, Eukaryota |
InParanoidi | Q64291 |
PhylomeDBi | Q64291 |
TreeFami | TF332742 |
Family and domain databases
Gene3Di | 1.20.5.1160, 1 hit |
InterProi | View protein in InterPro IPR018039, IF_conserved IPR039008, IF_rod_dom IPR042180, IF_rod_dom_coil1B IPR002957, Keratin_I |
PANTHERi | PTHR23239, PTHR23239, 1 hit |
Pfami | View protein in Pfam PF00038, Filament, 1 hit |
PRINTSi | PR01248, TYPE1KERATIN |
SMARTi | View protein in SMART SM01391, Filament, 1 hit |
PROSITEi | View protein in PROSITE PS00226, IF_ROD_1, 1 hit PS51842, IF_ROD_2, 1 hit |
s (2+)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 2 described isoforms and 1 potential isoform that is computationally mapped.Show allAlign All
Isoform 1 (identifier: Q64291-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MSLSVCTSAL SRRSSSQNGA AGRPWGASAS SVAGGYGGSA SGFGVGCGGL
60 70 80 90 100
FSAASMFGSS SGFSGGSAGC LPGLGSAYGG PLRGGAGGMG IGMGIAGSSG
110 120 130 140 150
GGSLCIFSGN DGGLLSGSEK ETMQNLNDRL ASYLGKVRSL EEANAELENK
160 170 180 190 200
IREWYETRRT RDAGSQSDYS KYYPLIEDLK NKIVSARVSN AQLLLQIDNA
210 220 230 240 250
RLAAEDFRMK YENELALRQT VEADINGLRR VLDELTLTRA DLEAQLETLT
260 270 280 290 300
EELAYMKKNH EEELQSFQAG GPGEVNVEMD AAPGVDLTKV LNEMRAQYEA
310 320 330 340 350
MAEQNRKDAE AWFLEKSGEL RKEISSNTEQ LQSSKSEVTD LKRMVQNLEI
360 370 380 390 400
ELQSQLAMKS SLEGSLAETE GGYCCQLSQV QQLIGSLEEQ LQQVRADAER
410 420 430 440 450
QNADHQRLLG VKARLEMEIE TYRRLLEGDS QGDGFDESSS LSVSKPQTPS
460 470 480
VDSSKDPNKT RKIKTVVQEI VNGEVVSSQV QELEEEM
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketZ4YJD9 | Z4YJD9_MOUSE | Keratin, type I cytoskeletal 12 | Krt12 | 487 | Annotation score: |
Sequence cautioni
The sequence AAA17792 differs from that shown. Reason: Frameshift.Curated
The sequence AAA52359 differs from that shown. Reason: Frameshift.Curated
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 9 | A → S in AAA17792 (PubMed:7508359).Curated | 1 | |
Sequence conflicti | 9 | A → S in AAA52359 (PubMed:7523376).Curated | 1 | |
Sequence conflicti | 89 | M → I in AAA17792 (PubMed:7508359).Curated | 1 | |
Sequence conflicti | 89 | M → I in AAA52359 (PubMed:7523376).Curated | 1 | |
Sequence conflicti | 93 – 101 | MGIAGSSGG → IGDSWNAGV in AAA17792 (PubMed:7508359).Curated | 9 | |
Sequence conflicti | 93 – 101 | MGIAGSSGG → IGDSWNAGV in AAA52359 (PubMed:7523376).Curated | 9 | |
Sequence conflicti | 318 | G → R in CAM21364 (PubMed:19468303).Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_002463 | 211 – 212 | YE → KL in isoform 2. Curated | 2 | |
Alternative sequenceiVSP_002464 | 213 – 487 | Missing in isoform 2. CuratedAdd BLAST | 275 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U02880 mRNA Translation: AAA17792.1 Frameshift. U08095 Genomic DNA Translation: AAA52359.1 Frameshift. AL591165 Genomic DNA Translation: CAM21364.1 BC131911 mRNA Translation: AAI31912.1 |
CCDSi | CCDS36306.1 [Q64291-1] |
PIRi | A55033 |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U02880 mRNA Translation: AAA17792.1 Frameshift. U08095 Genomic DNA Translation: AAA52359.1 Frameshift. AL591165 Genomic DNA Translation: CAM21364.1 BC131911 mRNA Translation: AAI31912.1 |
CCDSi | CCDS36306.1 [Q64291-1] |
PIRi | A55033 |
3D structure databases
SMRi | Q64291 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 10090.ENSMUSP00000017741 |
PTM databases
PhosphoSitePlusi | Q64291 |
Proteomic databases
PaxDbi | Q64291 |
PeptideAtlasi | Q64291 |
PRIDEi | Q64291 |
Organism-specific databases
MGIi | MGI:96687, Krt12 |
Phylogenomic databases
eggNOGi | ENOG502QTM6, Eukaryota |
InParanoidi | Q64291 |
PhylomeDBi | Q64291 |
TreeFami | TF332742 |
Enzyme and pathway databases
Reactomei | R-MMU-6805567, Keratinization R-MMU-6809371, Formation of the cornified envelope |
Miscellaneous databases
PROi | PR:Q64291 |
RNActi | Q64291, protein |
SOURCEi | Search... |
Family and domain databases
Gene3Di | 1.20.5.1160, 1 hit |
InterProi | View protein in InterPro IPR018039, IF_conserved IPR039008, IF_rod_dom IPR042180, IF_rod_dom_coil1B IPR002957, Keratin_I |
PANTHERi | PTHR23239, PTHR23239, 1 hit |
Pfami | View protein in Pfam PF00038, Filament, 1 hit |
PRINTSi | PR01248, TYPE1KERATIN |
SMARTi | View protein in SMART SM01391, Filament, 1 hit |
PROSITEi | View protein in PROSITE PS00226, IF_ROD_1, 1 hit PS51842, IF_ROD_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | K1C12_MOUSE | |
Accessioni | Q64291Primary (citable) accession number: Q64291 Secondary accession number(s): A2A514, A2RRZ0 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 1, 1997 |
Last sequence update: | April 3, 2007 | |
Last modified: | October 7, 2020 | |
This is version 137 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Direct protein sequencing, Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families