UniProtKB - Q64289 (NDF1_RAT)
Neurogenic differentiation factor 1
Neurod1
Functioni
Acts as a transcriptional activator: mediates transcriptional activation by binding to E box-containing promoter consensus core sequences 5'-CANNTG-3' (By similarity).
Associates with the p300/CBP transcription coactivator complex to stimulate transcription of the secretin gene as well as the gene encoding the cyclin-dependent kinase inhibitor CDKN1A (By similarity).
Contributes to the regulation of several cell differentiation pathways, like those that promote the formation of early retinal ganglion cells, inner ear sensory neurons, granule cells forming either the cerebellum or the dentate gyrus cell layer of the hippocampus, endocrine islet cells of the pancreas and enteroendocrine cells of the small intestine (By similarity).
Together with PAX6 or SIX3, is required for the regulation of amacrine cell fate specification (By similarity).
Also required for dendrite morphogenesis and maintenance in the cerebellar cortex (PubMed:14741104).
Associates with chromatin to enhancer regulatory elements in genes encoding key transcriptional regulators of neurogenesis (By similarity).
By similarity1 PublicationGO - Molecular functioni
- chromatin binding Source: UniProtKB
- DNA binding Source: RGD
- DNA-binding transcription activator activity, RNA polymerase II-specific Source: RGD
- DNA-binding transcription factor activity Source: RGD
- DNA-binding transcription factor activity, RNA polymerase II-specific Source: RGD
- double-stranded DNA binding Source: RGD
- E-box binding Source: RGD
- protein heterodimerization activity Source: RGD
- RNA polymerase II cis-regulatory region sequence-specific DNA binding Source: RGD
- RNA polymerase II-specific DNA-binding transcription factor binding Source: RGD
- sequence-specific DNA binding Source: RGD
- sequence-specific double-stranded DNA binding Source: RGD
GO - Biological processi
- amacrine cell differentiation Source: UniProtKB
- anterior/posterior pattern specification Source: RGD
- camera-type eye development Source: RGD
- cell fate commitment Source: RGD
- cellular response to glucose stimulus Source: UniProtKB
- cerebellum development Source: RGD
- dentate gyrus development Source: UniProtKB
- embryonic organ morphogenesis Source: RGD
- endocrine pancreas development Source: UniProtKB
- enteroendocrine cell differentiation Source: UniProtKB
- glucose homeostasis Source: RGD
- hindbrain development Source: RGD
- inner ear development Source: UniProtKB
- insulin secretion Source: RGD
- negative regulation of receptor signaling pathway via JAK-STAT Source: RGD
- negative regulation of type B pancreatic cell apoptotic process Source: RGD
- neuron development Source: InterPro
- neuron differentiation Source: GO_Central
- nitric oxide mediated signal transduction Source: RGD
- nucleocytoplasmic transport Source: UniProtKB
- pancreatic A cell fate commitment Source: RGD
- pancreatic PP cell fate commitment Source: RGD
- positive regulation of apoptotic process Source: UniProtKB
- positive regulation of cell differentiation Source: UniProtKB
- positive regulation of DNA-binding transcription factor activity Source: RGD
- positive regulation of neuron differentiation Source: RGD
- positive regulation of transcription, DNA-templated Source: UniProtKB
- positive regulation of transcription by RNA polymerase II Source: UniProtKB
- positive regulation of transcription regulatory region DNA binding Source: RGD
- regulation of intestinal epithelial structure maintenance Source: UniProtKB
- regulation of neuron differentiation Source: RGD
- regulation of transcription by RNA polymerase II Source: GO_Central
- response to glucose Source: RGD
- response to xenobiotic stimulus Source: RGD
- signal transduction involved in regulation of gene expression Source: RGD
Keywordsi
Molecular function | Activator, Developmental protein, DNA-binding |
Biological process | Differentiation, Neurogenesis, Transcription, Transcription regulation |
Names & Taxonomyi
Protein namesi | Recommended name: Neurogenic differentiation factor 1Short name: NeuroD1 Alternative name(s): Basic helix-loop-helix factor 1 Short name: BHF-1 |
Gene namesi | Name:Neurod1 Synonyms:Neurod |
Organismi | Rattus norvegicus (Rat) |
Taxonomic identifieri | 10116 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Rattus |
Proteomesi |
|
Organism-specific databases
RGDi | 3165, Neurod1 |
Subcellular locationi
Nucleus
- nucleus Source: UniProtKB
- RNA polymerase II transcription regulator complex Source: RGD
Other locations
- cytoplasm Source: UniProtKB
Keywords - Cellular componenti
Cytoplasm, NucleusPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 259 | S → A: No effect on subcellular location. 1 Publication | 1 | |
Mutagenesisi | 262 | T → A: No effect on subcellular location. 1 Publication | 1 | |
Mutagenesisi | 266 | S → A: No effect on subcellular location. 1 Publication | 1 | |
Mutagenesisi | 274 | S → A: Impairs translocation from the cytoplasm to the nucleus upon glucose stimulation. 1 Publication | 1 | |
Mutagenesisi | 274 | S → D: No effect on subcellular location. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000127384 | 1 – 357 | Neurogenic differentiation factor 1Add BLAST | 357 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 162 | PhosphoserineBy similarity | 1 | |
Modified residuei | 259 | PhosphoserineBy similarity | 1 | |
Modified residuei | 266 | PhosphoserineBy similarity | 1 | |
Modified residuei | 274 | Phosphoserine1 Publication | 1 | |
Modified residuei | 336 | Phosphoserine; by CaMK21 Publication | 1 |
Post-translational modificationi
Keywords - PTMi
PhosphoproteinProteomic databases
PaxDbi | Q64289 |
PTM databases
iPTMneti | Q64289 |
PhosphoSitePlusi | Q64289 |
Expressioni
Inductioni
Gene expression databases
Bgeei | ENSRNOG00000005609, Expressed in cerebellum and 11 other tissues |
Genevisiblei | Q64289, RN |
Interactioni
Subunit structurei
Efficient DNA-binding requires dimerization with another bHLH protein. Heterodimer with TCF3/E47; the heterodimer is inhibited in presence of ID2, but not NR0B2, to E-box element.
Interacts with EP300; the interaction is inhibited by NR0B2 (By similarity).
Interacts with RREB1 (By similarity).
Interacts with ATOH8 (By similarity).
By similarityGO - Molecular functioni
- protein heterodimerization activity Source: RGD
- RNA polymerase II-specific DNA-binding transcription factor binding Source: RGD
Protein-protein interaction databases
BioGRIDi | 248101, 2 interactors |
STRINGi | 10116.ENSRNOP00000007662 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 101 – 153 | bHLHPROSITE-ProRule annotationAdd BLAST | 53 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 94 | DisorderedSequence analysisAdd BLAST | 94 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 87 – 93 | Nuclear localization signalSequence analysis | 7 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 27 – 53 | Basic and acidic residuesSequence analysisAdd BLAST | 27 | |
Compositional biasi | 54 – 76 | Acidic residuesSequence analysisAdd BLAST | 23 |
Phylogenomic databases
eggNOGi | KOG3898, Eukaryota |
GeneTreei | ENSGT00940000160478 |
HOGENOMi | CLU_055134_0_0_1 |
InParanoidi | Q64289 |
OMAi | FKHEPAA |
OrthoDBi | 1096531at2759 |
PhylomeDBi | Q64289 |
TreeFami | TF315153 |
Family and domain databases
Gene3Di | 4.10.280.10, 1 hit |
InterProi | View protein in InterPro IPR011598, bHLH_dom IPR036638, HLH_DNA-bd_sf IPR032652, Neurod1 IPR022575, Neurogenic_DUF IPR016637, TF_bHLH_NeuroD |
PANTHERi | PTHR19290:SF88, PTHR19290:SF88, 1 hit |
Pfami | View protein in Pfam PF00010, HLH, 1 hit PF12533, Neuro_bHLH, 1 hit |
PIRSFi | PIRSF015618, bHLH_NeuroD, 1 hit |
SMARTi | View protein in SMART SM00353, HLH, 1 hit |
SUPFAMi | SSF47459, SSF47459, 1 hit |
PROSITEi | View protein in PROSITE PS50888, BHLH, 1 hit |
i Sequence
Sequence statusi: Complete.
10 20 30 40 50
MTKSYSESGL MGEPQPQGPP SWTDECLSSQ DEEHEADKKE DELEAMNAEE
60 70 80 90 100
DSLRNGGEEE DEDEDLEEEE EEEEEEDDQK PKRRGPKKKK MTKARLERFK
110 120 130 140 150
LRRMKANARE RNRMHGLNAA LDNLRKVVPC YSKTQKLSKI ETLRLAKNYI
160 170 180 190 200
WALSEILRSG KSPDLVSFVQ TLCKGLSQPT TNLVAGCLQL NPRTFLPEQN
210 220 230 240 250
PDMPPHLPTA SASFPVHPYS YQSPGLPSPP YGTMDSSHVF HVKPPPHAYS
260 270 280 290 300
AALEPFFESP LTDCTSPSFD GPLSPPLSIN GNFSFKHEPS TEFEKNYAFT
310 320 330 340 350
MHYPAATLAG PQSHGSIFSS GAAAPRCEIP IDNIMSFDSH SHHERVMSAQ
LNAIFHD
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | D82075 mRNA Translation: BAA11536.1 D82074 mRNA Translation: BAA11535.1 D82945 mRNA Translation: BAA81821.1 AF107728 mRNA Translation: AAD19609.1 BC092367 mRNA Translation: AAH92367.1 BC094526 mRNA Translation: AAH94526.1 U80603 mRNA Translation: AAB38744.1 |
PIRi | JC4703 |
RefSeqi | NP_062091.1, NM_019218.2 |
Genome annotation databases
Ensembli | ENSRNOT00000007662; ENSRNOP00000007662; ENSRNOG00000005609 |
GeneIDi | 29458 |
KEGGi | rno:29458 |
UCSCi | RGD:3165, rat |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | D82075 mRNA Translation: BAA11536.1 D82074 mRNA Translation: BAA11535.1 D82945 mRNA Translation: BAA81821.1 AF107728 mRNA Translation: AAD19609.1 BC092367 mRNA Translation: AAH92367.1 BC094526 mRNA Translation: AAH94526.1 U80603 mRNA Translation: AAB38744.1 |
PIRi | JC4703 |
RefSeqi | NP_062091.1, NM_019218.2 |
3D structure databases
AlphaFoldDBi | Q64289 |
SMRi | Q64289 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 248101, 2 interactors |
STRINGi | 10116.ENSRNOP00000007662 |
PTM databases
iPTMneti | Q64289 |
PhosphoSitePlusi | Q64289 |
Proteomic databases
PaxDbi | Q64289 |
Genome annotation databases
Ensembli | ENSRNOT00000007662; ENSRNOP00000007662; ENSRNOG00000005609 |
GeneIDi | 29458 |
KEGGi | rno:29458 |
UCSCi | RGD:3165, rat |
Organism-specific databases
CTDi | 4760 |
RGDi | 3165, Neurod1 |
Phylogenomic databases
eggNOGi | KOG3898, Eukaryota |
GeneTreei | ENSGT00940000160478 |
HOGENOMi | CLU_055134_0_0_1 |
InParanoidi | Q64289 |
OMAi | FKHEPAA |
OrthoDBi | 1096531at2759 |
PhylomeDBi | Q64289 |
TreeFami | TF315153 |
Miscellaneous databases
PROi | PR:Q64289 |
Gene expression databases
Bgeei | ENSRNOG00000005609, Expressed in cerebellum and 11 other tissues |
Genevisiblei | Q64289, RN |
Family and domain databases
Gene3Di | 4.10.280.10, 1 hit |
InterProi | View protein in InterPro IPR011598, bHLH_dom IPR036638, HLH_DNA-bd_sf IPR032652, Neurod1 IPR022575, Neurogenic_DUF IPR016637, TF_bHLH_NeuroD |
PANTHERi | PTHR19290:SF88, PTHR19290:SF88, 1 hit |
Pfami | View protein in Pfam PF00010, HLH, 1 hit PF12533, Neuro_bHLH, 1 hit |
PIRSFi | PIRSF015618, bHLH_NeuroD, 1 hit |
SMARTi | View protein in SMART SM00353, HLH, 1 hit |
SUPFAMi | SSF47459, SSF47459, 1 hit |
PROSITEi | View protein in PROSITE PS50888, BHLH, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | NDF1_RAT | |
Accessioni | Q64289Primary (citable) accession number: Q64289 Secondary accession number(s): Q569P0 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 1, 1997 |
Last sequence update: | November 1, 1996 | |
Last modified: | May 25, 2022 | |
This is version 154 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |