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Protein

Neurogenic differentiation factor 1

Gene

Neurod1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Acts as a transcriptional activator: mediates transcriptional activation by binding to E box-containing promoter consensus core sequences 5'-CANNTG-3'. Associates with the p300/CBP transcription coactivator complex to stimulate transcription of the secretin gene as well as the gene encoding the cyclin-dependent kinase inhibitor CDKN1A. Contributes to the regulation of several cell differentiation pathways, like those that promote the formation of early retinal ganglion cells, inner ear sensory neurons, granule cells forming either the cerebellum or the dentate gyrus cell layer of the hippocampus, endocrine islet cells of the pancreas and enteroendocrine cells of the small intestine. Together with PAX6 or SIX3, is required for the regulation of amacrine cell fate specification. Also required for dendrite morphogenesis and maintenance in the cerebellar cortex. Associates with chromatin to enhancer regulatory elements in genes encoding key transcriptional regulators of neurogenesis (By similarity).By similarity1 Publication

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionActivator, Developmental protein, DNA-binding
Biological processDifferentiation, Neurogenesis, Transcription, Transcription regulation

Names & Taxonomyi

Protein namesi
Recommended name:
Neurogenic differentiation factor 1
Short name:
NeuroD1
Alternative name(s):
Basic helix-loop-helix factor 1
Short name:
BHF-1
Gene namesi
Name:Neurod1
Synonyms:Neurod
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 3

Organism-specific databases

RGDi3165 Neurod1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi259S → A: No effect on subcellular location. 1 Publication1
Mutagenesisi262T → A: No effect on subcellular location. 1 Publication1
Mutagenesisi266S → A: No effect on subcellular location. 1 Publication1
Mutagenesisi274S → A: Impairs translocation from the cytoplasm to the nucleus upon glucose stimulation. 1 Publication1
Mutagenesisi274S → D: No effect on subcellular location. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001273841 – 357Neurogenic differentiation factor 1Add BLAST357

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei162PhosphoserineBy similarity1
Modified residuei259PhosphoserineBy similarity1
Modified residuei266PhosphoserineBy similarity1
Modified residuei274Phosphoserine1 Publication1
Modified residuei336Phosphoserine; by CaMK21 Publication1

Post-translational modificationi

Phosphorylated by MAPK1; phosphorylation regulates heterodimerization and DNA-binding activities. Phosphorylation on Ser-266 and Ser-274 increases transactivation on the insulin promoter in glucose-stimulated insulinoma cells (By similarity). Phosphorylated. In islet cells, phosphorylated on Ser-274 upon glucose stimulation; which may be required for nuclear localization. In activated neurons, phosphorylated on Ser-336 by CaMK2; which promotes dendritic growth.By similarity2 Publications

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ64289
PRIDEiQ64289

PTM databases

iPTMnetiQ64289
PhosphoSitePlusiQ64289

Expressioni

Inductioni

Up-regulated by fentanyl. Down-regulated by miR-190.1 Publication

Gene expression databases

BgeeiENSRNOG00000005609 Expressed in 3 organ(s), highest expression level in Ammon's horn
GenevisibleiQ64289 RN

Interactioni

Subunit structurei

Heterodimer with TCF3/E47; the heterodimer is inhibited in presence of ID2, but not NR0B2, to E-box element. Interacts (via helix-loop-helix motif domain) with EP300 (via C-terminus); the interaction is inhibited by NR0B2. Interacts with TCF3; the interaction is inhibited by ID2. Interacts with RREB1 (By similarity). Efficient DNA-binding requires dimerization with another bHLH protein. Heterodimer with TCF3/E47. Interacts with ATOH8 (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

BioGridi248101, 2 interactors
STRINGi10116.ENSRNOP00000007662

Structurei

3D structure databases

ProteinModelPortaliQ64289
SMRiQ64289
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini101 – 153bHLHPROSITE-ProRule annotationAdd BLAST53

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi87 – 93Nuclear localization signalSequence analysis7

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi58 – 77Glu-rich (acidic)Add BLAST20
Compositional biasi67 – 76Poly-Glu10
Compositional biasi87 – 90Poly-Lys4

Phylogenomic databases

eggNOGiENOG410IQ4I Eukaryota
ENOG410Z6FG LUCA
GeneTreeiENSGT00680000099860
HOGENOMiHOG000049256
HOVERGENiHBG000250
InParanoidiQ64289
KOiK08033
OMAiEEHETDK
OrthoDBiEOG091G15AI
PhylomeDBiQ64289
TreeFamiTF315153

Family and domain databases

CDDicd00083 HLH, 1 hit
Gene3Di4.10.280.10, 1 hit
InterProiView protein in InterPro
IPR011598 bHLH_dom
IPR036638 HLH_DNA-bd_sf
IPR032652 Neurod1
IPR022575 Neurogenic_DUF
IPR016637 TF_bHLH_NeuroD
PANTHERiPTHR19290:SF88 PTHR19290:SF88, 1 hit
PfamiView protein in Pfam
PF00010 HLH, 1 hit
PF12533 Neuro_bHLH, 1 hit
PIRSFiPIRSF015618 bHLH_NeuroD, 1 hit
SMARTiView protein in SMART
SM00353 HLH, 1 hit
SUPFAMiSSF47459 SSF47459, 1 hit
PROSITEiView protein in PROSITE
PS50888 BHLH, 1 hit

Sequencei

Sequence statusi: Complete.

Q64289-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MTKSYSESGL MGEPQPQGPP SWTDECLSSQ DEEHEADKKE DELEAMNAEE
60 70 80 90 100
DSLRNGGEEE DEDEDLEEEE EEEEEEDDQK PKRRGPKKKK MTKARLERFK
110 120 130 140 150
LRRMKANARE RNRMHGLNAA LDNLRKVVPC YSKTQKLSKI ETLRLAKNYI
160 170 180 190 200
WALSEILRSG KSPDLVSFVQ TLCKGLSQPT TNLVAGCLQL NPRTFLPEQN
210 220 230 240 250
PDMPPHLPTA SASFPVHPYS YQSPGLPSPP YGTMDSSHVF HVKPPPHAYS
260 270 280 290 300
AALEPFFESP LTDCTSPSFD GPLSPPLSIN GNFSFKHEPS TEFEKNYAFT
310 320 330 340 350
MHYPAATLAG PQSHGSIFSS GAAAPRCEIP IDNIMSFDSH SHHERVMSAQ

LNAIFHD
Length:357
Mass (Da):40,001
Last modified:November 1, 1996 - v1
Checksum:iF773637E64D3E99E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D82075 mRNA Translation: BAA11536.1
D82074 mRNA Translation: BAA11535.1
D82945 mRNA Translation: BAA81821.1
AF107728 mRNA Translation: AAD19609.1
BC092367 mRNA Translation: AAH92367.1
BC094526 mRNA Translation: AAH94526.1
U80603 mRNA Translation: AAB38744.1
PIRiJC4703
RefSeqiNP_062091.1, NM_019218.2
UniGeneiRn.44289

Genome annotation databases

EnsembliENSRNOT00000007662; ENSRNOP00000007662; ENSRNOG00000005609
GeneIDi29458
KEGGirno:29458
UCSCiRGD:3165 rat

Similar proteinsi

Entry informationi

Entry nameiNDF1_RAT
AccessioniPrimary (citable) accession number: Q64289
Secondary accession number(s): Q569P0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: September 12, 2018
This is version 135 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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