Neurod1

Functionⁱ

Acts as a transcriptional activator: mediates transcriptional activation by binding to E box-containing promoter consensus core sequences 5'-CANNTG-3'. Associates with the p300/CBP transcription coactivator complex to stimulate transcription of the secretin gene as well as the gene encoding the cyclin-dependent kinase inhibitor CDKN1A. Contributes to the regulation of several cell differentiation pathways, like those that promote the formation of early retinal ganglion cells, inner ear sensory neurons, granule cells forming either the cerebellum or the dentate gyrus cell layer of the hippocampus, endocrine islet cells of the pancreas and enteroendocrine cells of the small intestine. Together with PAX6 or SIX3, is required for the regulation of amacrine cell fate specification. Also required for dendrite morphogenesis and maintenance in the cerebellar cortex. Associates with chromatin to enhancer regulatory elements in genes encoding key transcriptional regulators of neurogenesis (By similarity).By similarity1 Publication

GO - Molecular functionⁱ

chromatin binding Source: UniProtKB
double-stranded DNA binding
Source: RGD
Inferred from direct assayⁱ
- 10101232
E-box binding Source: Ensembl
protein heterodimerization activity Source: Ensembl
RNA polymerase II activating transcription factor binding Source: Ensembl
transcriptional activator activity, RNA polymerase II proximal promoter sequence-specific DNA binding Source: Ensembl
transcription coactivator activity Source: Ensembl

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

amacrine cell differentiation Source: UniProtKB
anterior/posterior pattern specification Source: Ensembl
cellular response to glucose stimulus
Source: UniProtKB
Inferred from direct assayⁱ
- 12297313
cerebellum development
Source: RGD
Inferred from expression patternⁱ
- 12242038
dentate gyrus development Source: UniProtKB
embryonic organ morphogenesis Source: Ensembl
endocrine pancreas development Source: UniProtKB
enteroendocrine cell differentiation Source: UniProtKB
inner ear development Source: UniProtKB
insulin secretion Source: Ensembl
negative regulation of JAK-STAT cascade Source: Ensembl
negative regulation of type B pancreatic cell apoptotic process Source: Ensembl
neuron development Source: InterPro
nitric oxide mediated signal transduction Source: Ensembl
nucleocytoplasmic transport
Source: UniProtKB
Inferred from direct assayⁱ
- 12297313
pancreatic A cell fate commitment Source: Ensembl
pancreatic PP cell fate commitment Source: Ensembl
positive regulation of apoptotic process Source: UniProtKB
positive regulation of cell differentiation Source: UniProtKB
positive regulation of DNA-binding transcription factor activity Source: Ensembl
positive regulation of neuron differentiation
Source: RGD
Inferred from direct assayⁱ
- 10101232
positive regulation of transcription, DNA-templated Source: UniProtKB
positive regulation of transcription by RNA polymerase II
Source: UniProtKB
Inferred from direct assayⁱ
- 12297313
positive regulation of transcription regulatory region DNA binding Source: Ensembl
regulation of cell cycle arrest Source: UniProtKB
regulation of intestinal epithelial structure maintenance Source: UniProtKB
response to drug
Source: RGD
Inferred from expression patternⁱ
- 16635253
signal transduction involved in regulation of gene expression Source: Ensembl

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Molecular function	Activator, Developmental protein, DNA-binding
Biological process	Differentiation, Neurogenesis, Transcription, Transcription regulation

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: Neurogenic differentiation factor 1 Short name: NeuroD1 Alternative name(s): Basic helix-loop-helix factor 1 Short name: BHF-1
Gene namesⁱ	Name:Neurod1 Synonyms:Neurod
Organismⁱ	Rattus norvegicus (Rat)
Taxonomic identifierⁱ	10116 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Dipnotetrapodomorpha › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Boreoeutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Rattus
Proteomesⁱ	UP000002494 Componentⁱ: Chromosome 3

Organism-specific databases

Rat genome database More...RGDⁱ	3165 Neurod1

RGDⁱ

3165 Neurod1

Keywords - Cellular componentⁱ

Cytoplasm, Nucleus

Pathology & Biotechⁱ

Mutagenesis

Feature key	Position(s)	DescriptionActions	Length
Mutagenesisⁱ	259	S → A: No effect on subcellular location. 1 Publication	1
Mutagenesisⁱ	262	T → A: No effect on subcellular location. 1 Publication	1
Mutagenesisⁱ	266	S → A: No effect on subcellular location. 1 Publication	1
Mutagenesisⁱ	274	S → A: Impairs translocation from the cytoplasm to the nucleus upon glucose stimulation. 1 Publication	1
Mutagenesisⁱ	274	S → D: No effect on subcellular location. 1 Publication	1

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
ChainⁱPRO_0000127384	1 – 357	Neurogenic differentiation factor 1Add BLAST		357

Amino acid modifications

Feature key	Position(s)	DescriptionActions	Length
Modified residueⁱ	162	PhosphoserineBy similarity	1
Modified residueⁱ	259	PhosphoserineBy similarity	1
Modified residueⁱ	266	PhosphoserineBy similarity	1
Modified residueⁱ	274	Phosphoserine1 Publication	1
Modified residueⁱ	336	Phosphoserine; by CaMK21 Publication	1

Post-translational modificationⁱ

Phosphorylated by MAPK1; phosphorylation regulates heterodimerization and DNA-binding activities. Phosphorylation on Ser-266 and Ser-274 increases transactivation on the insulin promoter in glucose-stimulated insulinoma cells (By similarity). Phosphorylated. In islet cells, phosphorylated on Ser-274 upon glucose stimulation; which may be required for nuclear localization. In activated neurons, phosphorylated on Ser-336 by CaMK2; which promotes dendritic growth.By similarity2 Publications

Keywords - PTMⁱ

Phosphoprotein

Proteomic databases

PaxDbⁱ	Q64289
PRIDEⁱ	Q64289

PTM databases

iPTMnetⁱ	Q64289
PhosphoSitePlusⁱ	Q64289

Expressionⁱ

Inductionⁱ

Up-regulated by fentanyl. Down-regulated by miR-190.1 Publication

Gene expression databases

Bgeeⁱ	ENSRNOG00000005609 Expressed in 3 organ(s), highest expression level in Ammon's horn
Genevisibleⁱ	Q64289 RN

Interactionⁱ

Subunit structureⁱ

Heterodimer with TCF3/E47; the heterodimer is inhibited in presence of ID2, but not NR0B2, to E-box element. Interacts (via helix-loop-helix motif domain) with EP300 (via C-terminus); the interaction is inhibited by NR0B2. Interacts with TCF3; the interaction is inhibited by ID2. Interacts with RREB1 (By similarity). Efficient DNA-binding requires dimerization with another bHLH protein. Heterodimer with TCF3/E47. Interacts with ATOH8 (By similarity).By similarity

GO - Molecular functionⁱ

protein heterodimerization activity Source: Ensembl
RNA polymerase II activating transcription factor binding Source: Ensembl

View the complete GO annotation on QuickGO ...

Protein-protein interaction databases

BioGridⁱ	248101, 2 interactors
STRINGⁱ	10116.ENSRNOP00000007662

Structureⁱ

3D structure databases

ProteinModelPortalⁱ	Q64289
SMRⁱ	Q64289
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Family & Domainsⁱ

Domains and Repeats

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Domainⁱ	101 – 153	bHLHPROSITE-ProRule annotationAdd BLAST		53

Motif

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Motifⁱ	87 – 93	Nuclear localization signalSequence analysis		7

Compositional bias

Feature key	Position(s)	DescriptionActions	Length
Compositional biasⁱ	58 – 77	Glu-rich (acidic)Add BLAST	20
Compositional biasⁱ	67 – 76	Poly-Glu	10
Compositional biasⁱ	87 – 90	Poly-Lys	4

Phylogenomic databases

eggNOGⁱ	ENOG410IQ4I Eukaryota ENOG410Z6FG LUCA
Ensembl GeneTree More...GeneTreeⁱ	ENSGT00680000099860
HOGENOMⁱ	HOG000049256
HOVERGENⁱ	HBG000250
InParanoidⁱ	Q64289
KEGG Orthology (KO) More...KOⁱ	K08033
OMAⁱ	EEHETDK
Database of Orthologous Groups More...OrthoDBⁱ	EOG091G15AI
PhylomeDBⁱ	Q64289
TreeFamⁱ	TF315153

Family and domain databases

Conserved Domains Database More...CDDⁱ	cd00083 HLH, 1 hit
Gene3Dⁱ	4.10.280.10, 1 hit
InterProⁱ	View protein in InterPro IPR011598 bHLH_dom IPR036638 HLH_DNA-bd_sf IPR032652 Neurod1 IPR022575 Neurogenic_DUF IPR016637 TF_bHLH_NeuroD
PANTHERⁱ	PTHR19290:SF88 PTHR19290:SF88, 1 hit
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF00010 HLH, 1 hit PF12533 Neuro_bHLH, 1 hit
PIRSFⁱ	PIRSF015618 bHLH_NeuroD, 1 hit
SMARTⁱ	View protein in SMART SM00353 HLH, 1 hit
SUPFAMⁱ	SSF47459 SSF47459, 1 hit
PROSITEⁱ	View protein in PROSITE PS50888 BHLH, 1 hit

Sequenceⁱ

Sequence statusⁱ: Complete.

Q64289-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
MTKSYSESGL MGEPQPQGPP SWTDECLSSQ DEEHEADKKE DELEAMNAEE 
        60         70         80         90        100
DSLRNGGEEE DEDEDLEEEE EEEEEEDDQK PKRRGPKKKK MTKARLERFK 
       110        120        130        140        150
LRRMKANARE RNRMHGLNAA LDNLRKVVPC YSKTQKLSKI ETLRLAKNYI 
       160        170        180        190        200
WALSEILRSG KSPDLVSFVQ TLCKGLSQPT TNLVAGCLQL NPRTFLPEQN 
       210        220        230        240        250
PDMPPHLPTA SASFPVHPYS YQSPGLPSPP YGTMDSSHVF HVKPPPHAYS 
       260        270        280        290        300
AALEPFFESP LTDCTSPSFD GPLSPPLSIN GNFSFKHEPS TEFEKNYAFT 
       310        320        330        340        350
MHYPAATLAG PQSHGSIFSS GAAAPRCEIP IDNIMSFDSH SHHERVMSAQ 

LNAIFHD

Length:357

Mass (Da):40,001

Last modified:November 1, 1996 - v1

Checksum:ⁱF773637E64D3E99E

GO

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	D82075 mRNA Translation: BAA11536.1 D82074 mRNA Translation: BAA11535.1 D82945 mRNA Translation: BAA81821.1 AF107728 mRNA Translation: AAD19609.1 BC092367 mRNA Translation: AAH92367.1 BC094526 mRNA Translation: AAH94526.1 U80603 mRNA Translation: AAB38744.1
PIRⁱ	JC4703
NCBI Reference Sequences More...RefSeqⁱ	NP_062091.1, NM_019218.2
UniGeneⁱ	Rn.44289

Genome annotation databases

Ensemblⁱ	ENSRNOT00000007662; ENSRNOP00000007662; ENSRNOG00000005609
GeneIDⁱ	29458
KEGGⁱ	rno:29458
UCSC genome browser More...UCSCⁱ	RGD:3165 rat

Similar proteinsⁱ

90% Identity
50% Identity

Protein	Similar proteins		Species	Score	Length	Source
Q64289	Neurogenic differentiation factor 1	)	MOUSE		357	UniRef90_Q60867
Neurogenic differentiation factor 1		HUMAN		356
Neurogenic differentiation factor 1		MESAU		355
Neurogenic differentiation factor		PTEAL		362
Neurogenic differentiation factor		PELSI		357
+101

Protein	Similar proteins		Species	Score	Length	Source
Q64289	Neurogenic differentiation factor 1	)	MOUSE		357	UniRef50_Q60867
Neurogenic differentiation factor 1		HUMAN		356
Neurogenic differentiation factor 1		MESAU		355
Neurogenic differentiation factor		PTEAL		362
Neurogenic differentiation factor		SARHA		357
+224

Entry informationⁱ

Entry nameⁱ	NDF1_RAT
Accessionⁱ	Q64289Primary (citable) accession number: Q64289 Secondary accession number(s): Q569P0
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
	Last sequence update:	November 1, 1996
	Last modified:	September 12, 2018
	This is version 135 of the entry and version 1 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Chordata Protein Annotation Program

Miscellaneousⁱ

Keywords - Technical termⁱ

Complete proteome, Reference proteome

UniProtKB

UniParc

Help

UniRef

Proteomes

Annotation systems

Supporting data

UniProtKB - Q64289 (NDF1_RAT)

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Neurogenic differentiation factor 1

Select a section on the left to see content.

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

Organism-specific databases

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Nucleus

Other locations

Nucleus

Other locations

Keywords - Cellular componenti

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

Mutagenesis

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Amino acid modifications

Keywords - PTMi

Proteomic databases

PTM databases

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

Gene expression databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

GO - Molecular functioni

Protein-protein interaction databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

Motif

Compositional bias

Phylogenomic databases

Family and domain databases

Sequence databases

Genome annotation databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

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Functionⁱ

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

Keywords - Cellular componentⁱ

Pathology & Biotechⁱ

PTM / Processingⁱ

Keywords - PTMⁱ

Expressionⁱ

Interactionⁱ

GO - Molecular functionⁱ

Structureⁱ

Family & Domainsⁱ

Entry informationⁱ

Miscellaneousⁱ

Keywords - Technical termⁱ