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Protein

Interferon-induced protein with tetratricopeptide repeats 1

Gene

Ifit1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Interferon-induced antiviral RNA-binding protein that specifically binds single-stranded RNA bearing a 5'-triphosphate group (PPP-RNA), thereby acting as a sensor of viral single-stranded RNAs and inhibiting expression of viral messenger RNAs. Single-stranded PPP-RNAs, which lack 2'-O-methylation of the 5' cap and bear a 5'-triphosphate group instead, are specific from viruses, providing a molecular signature to distinguish between self and non-self mRNAs by the host during viral infection. Directly binds PPP-RNA in a non-sequence-specific manner. Viruses evolved several ways to evade this restriction system such as encoding their own 2'-O-methylase for their mRNAs or by stealing host cap containing the 2'-O-methylation (cap snatching mechanism).2 Publications

GO - Molecular functioni

GO - Biological processi

  • cellular response to interferon-alpha Source: MGI
  • cellular response to interferon-beta Source: MGI
  • defense response to virus Source: GO_Central
  • innate immune response Source: UniProtKB-KW
  • response to bacterium Source: MGI
  • response to virus Source: UniProtKB

Keywordsi

Molecular functionRNA-binding
Biological processAntiviral defense, Immunity, Innate immunity

Names & Taxonomyi

Protein namesi
Recommended name:
Interferon-induced protein with tetratricopeptide repeats 1
Short name:
IFIT-1
Alternative name(s):
Glucocorticoid-attenuated response gene 16 protein
Short name:
GARG-16
Interferon-induced 56 kDa protein
Short name:
IFI-56K
Short name:
P56
Gene namesi
Name:Ifit1
Synonyms:Garg16, Ifi56, Isg56
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 19

Organism-specific databases

MGIiMGI:99450 Ifit1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001063451 – 463Interferon-induced protein with tetratricopeptide repeats 1Add BLAST463

Post-translational modificationi

Phosphorylated.By similarity
ISGylated.By similarity

Keywords - PTMi

Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiQ64282
MaxQBiQ64282
PaxDbiQ64282
PRIDEiQ64282

PTM databases

iPTMnetiQ64282
PhosphoSitePlusiQ64282
SwissPalmiQ64282

Expressioni

Inductioni

By type I interferons, dsRNAs and viruses.1 Publication

Gene expression databases

BgeeiENSMUSG00000034459 Expressed in 164 organ(s), highest expression level in intestine
GenevisibleiQ64282 MM

Interactioni

Subunit structurei

Component of an interferon-dependent multiprotein complex, at least composed of IFIT1, IFIT2 and IFIT3. Interacts with IFIT2 and IFIT3 (By similarity). Interacts with EIF3E (By similarity). Interacts (via TPR repeats 1-4) with RPL15 (By similarity). Interacts with TMEM173/MITA and disrupts its interaction with MAVS or TBK1 (By similarity). Interacts with EIF3C. Interacts (via TPR repeats 4-7) with EEF1A1.By similarity2 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei34Interaction with PPP-RNABy similarity1
Sitei42Interaction with PPP-RNABy similarity1
Sitei148Interaction with PPP-RNABy similarity1
Sitei184Interaction with PPP-RNABy similarity1
Sitei243Interaction with PPP-RNABy similarity1
Sitei281Interaction with the 5'-triphosphate group of PPP-RNABy similarity1

Protein-protein interaction databases

IntActiQ64282, 1 interactor
MINTiQ64282
STRINGi10090.ENSMUSP00000099888

Structurei

3D structure databases

ProteinModelPortaliQ64282
SMRiQ64282
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati52 – 85TPR 1Add BLAST34
Repeati92 – 126TPR 2Add BLAST35
Repeati136 – 171TPR 3Add BLAST36
Repeati174 – 207TPR 4Add BLAST34
Repeati209 – 241TPR 5Add BLAST33
Repeati242 – 275TPR 6Add BLAST34
Repeati294 – 328TPR 7Add BLAST35
Repeati329 – 362TPR 8Add BLAST34
Repeati367 – 401TPR 9Add BLAST35
Repeati426 – 459TPR 10Add BLAST34

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni247 – 253Interaction with the 5'-triphosphate group of PPP-RNABy similarity7

Domaini

RNA recognition is mediated by a convoluted intramolecular fold of the TPR repeats (TPR eddy), which scaffolds unique additional helices that form an RNA binding cleft.By similarity

Sequence similaritiesi

Belongs to the IFIT family.Curated

Keywords - Domaini

Repeat, TPR repeat

Phylogenomic databases

eggNOGiKOG1124 Eukaryota
COG0457 LUCA
GeneTreeiENSGT00390000013876
HOGENOMiHOG000001558
HOVERGENiHBG066330
InParanoidiQ64282
KOiK14217
OMAiNRIWEEI
OrthoDBiEOG091G06GX
TreeFamiTF342671

Family and domain databases

Gene3Di1.25.40.10, 3 hits
InterProiView protein in InterPro
IPR013026 TPR-contain_dom
IPR011990 TPR-like_helical_dom_sf
IPR019734 TPR_repeat
PfamiView protein in Pfam
PF13181 TPR_8, 1 hit
SMARTiView protein in SMART
SM00028 TPR, 6 hits
SUPFAMiSSF48452 SSF48452, 3 hits
PROSITEiView protein in PROSITE
PS50005 TPR, 4 hits
PS50293 TPR_REGION, 3 hits

Sequencei

Sequence statusi: Complete.

Q64282-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MGENADGDQV MENLLQLRCH FTWKLLFENN DIPDLEVRIS EQVQFLDIKN
60 70 80 90 100
PLGMHNLLAY VRHLKGQQDE ALQSLKEAEA LIQSEQLSKR SLATWGNCAW
110 120 130 140 150
LHYHRGSLAE AQIYLDKVEK VCKEFSSPFR YRLECAEMDC EEGWALLKCG
160 170 180 190 200
GGNYKQAMAC FAKALKVEPE NPEYNTGYAV VAYRQDLDDN FISLEPLRKA
210 220 230 240 250
VRLNPEDPYL KVLLALKLQD LGEHVEAEAH IEEALSSTSC QSYVIRYAAK
260 270 280 290 300
YFRRKHRVDK ALHLLNRALQ ASPSSGYLHY QKGLCYKQQI SQLRTSRNRQ
310 320 330 340 350
PRRQDNVQEL AQQAIHEFQE TLKLRPTFEM AYVCMAEVQA EIHQYEEAER
360 370 380 390 400
NFQKALNNKT LVAHIEQDIH LRYGRFLQFH KQSEDKAITL YLKGLKVEEK
410 420 430 440 450
SFAWRKLLTA LEKVAERRVC QNVHLVESTS LLGLVYKLKG QEKNALFYYE
460
KALRLTGEMN PAF
Length:463
Mass (Da):53,737
Last modified:July 27, 2011 - v2
Checksum:i166FAB371F7B6D2A
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti113I → V in AAB33831 (PubMed:7896268).Curated1
Sequence conflicti113I → V in AAC52625 (PubMed:8660659).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S77715, S77714 Genomic DNA Translation: AAB33831.2
U43084 mRNA Translation: AAC52625.1
AK150352 mRNA Translation: BAE29489.1
AK150424 mRNA Translation: BAE29547.1
AK150609 mRNA Translation: BAE29700.1
AK151125 mRNA Translation: BAE30133.1
AK151158 mRNA Translation: BAE30163.1
AK151278 mRNA Translation: BAE30265.1
AK151298 mRNA Translation: BAE30281.1
AK153184 mRNA Translation: BAE31785.1
AK157848 mRNA Translation: BAE34228.1
CT010226 mRNA Translation: CAJ18434.1
CH466534 Genomic DNA Translation: EDL41756.1
BC003768 mRNA Translation: AAH03768.1
CCDSiCCDS29766.1
PIRiB55508
RefSeqiNP_032357.2, NM_008331.3
UniGeneiMm.439751

Genome annotation databases

EnsembliENSMUST00000102824; ENSMUSP00000099888; ENSMUSG00000034459
GeneIDi15957
KEGGimmu:15957
UCSCiuc008hgt.1 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S77715, S77714 Genomic DNA Translation: AAB33831.2
U43084 mRNA Translation: AAC52625.1
AK150352 mRNA Translation: BAE29489.1
AK150424 mRNA Translation: BAE29547.1
AK150609 mRNA Translation: BAE29700.1
AK151125 mRNA Translation: BAE30133.1
AK151158 mRNA Translation: BAE30163.1
AK151278 mRNA Translation: BAE30265.1
AK151298 mRNA Translation: BAE30281.1
AK153184 mRNA Translation: BAE31785.1
AK157848 mRNA Translation: BAE34228.1
CT010226 mRNA Translation: CAJ18434.1
CH466534 Genomic DNA Translation: EDL41756.1
BC003768 mRNA Translation: AAH03768.1
CCDSiCCDS29766.1
PIRiB55508
RefSeqiNP_032357.2, NM_008331.3
UniGeneiMm.439751

3D structure databases

ProteinModelPortaliQ64282
SMRiQ64282
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ64282, 1 interactor
MINTiQ64282
STRINGi10090.ENSMUSP00000099888

PTM databases

iPTMnetiQ64282
PhosphoSitePlusiQ64282
SwissPalmiQ64282

Proteomic databases

EPDiQ64282
MaxQBiQ64282
PaxDbiQ64282
PRIDEiQ64282

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000102824; ENSMUSP00000099888; ENSMUSG00000034459
GeneIDi15957
KEGGimmu:15957
UCSCiuc008hgt.1 mouse

Organism-specific databases

CTDi3434
MGIiMGI:99450 Ifit1

Phylogenomic databases

eggNOGiKOG1124 Eukaryota
COG0457 LUCA
GeneTreeiENSGT00390000013876
HOGENOMiHOG000001558
HOVERGENiHBG066330
InParanoidiQ64282
KOiK14217
OMAiNRIWEEI
OrthoDBiEOG091G06GX
TreeFamiTF342671

Miscellaneous databases

PROiPR:Q64282
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000034459 Expressed in 164 organ(s), highest expression level in intestine
GenevisibleiQ64282 MM

Family and domain databases

Gene3Di1.25.40.10, 3 hits
InterProiView protein in InterPro
IPR013026 TPR-contain_dom
IPR011990 TPR-like_helical_dom_sf
IPR019734 TPR_repeat
PfamiView protein in Pfam
PF13181 TPR_8, 1 hit
SMARTiView protein in SMART
SM00028 TPR, 6 hits
SUPFAMiSSF48452 SSF48452, 3 hits
PROSITEiView protein in PROSITE
PS50005 TPR, 4 hits
PS50293 TPR_REGION, 3 hits
ProtoNetiSearch...

Entry informationi

Entry nameiIFIT1_MOUSE
AccessioniPrimary (citable) accession number: Q64282
Secondary accession number(s): Q99L77
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: July 27, 2011
Last modified: November 7, 2018
This is version 132 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
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Main funding by: National Institutes of Health

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