UniProtKB - Q64281 (LRB4A_MOUSE)
Leukocyte immunoglobulin-like receptor subfamily B member 4A
Lilrb4a
Functioni
Inhibitory receptor involved in the down-regulation of the immune response (PubMed:10026201, PubMed:24935931).
Receptor for FN1 (PubMed:34089617).
Receptor for integrin ITGAV/ITGB3 (PubMed:11323698).
Inhibits IgE-mediated mast cell activation, at least in part through interaction with ITGAV/ITGB3 (PubMed:8855262, PubMed:10026201, PubMed:11457897, PubMed:11323698).
Also inhibits KITLG/SCF-mediated mast cell activation (PubMed:12884301).
Through interaction with ITGAV/ITGB3, inhibits antibody production by memory and marginal zone B cells, probably by suppressing their differentiation into plasma cells (PubMed:24935931).
Inhibits IFNG production by CD8 T cells, CD4 T cells and natural killer cells (PubMed:12682239).
Inhibits antigen presentation by dendritic cells to T cells, preventing T cell activation (PubMed:18792399).
Inhibits lipopolysaccharide-mediated neutrophil-dependent vascular injury (PubMed:14557414).
Suppresses the allergic inflammatory response by inhibiting infiltration of neutrophils and eosinophils and preventing mast cell degranulation (PubMed:17761953).
Inhibits lysis by natural killer cells (PubMed:8977169).
12 PublicationsGO - Molecular functioni
- fibronectin binding Source: UniProtKB
- integrin binding Source: ARUK-UCL
- phosphatase binding Source: UniProtKB
- protein phosphatase binding Source: UniProtKB
- signaling receptor inhibitor activity Source: MGI
- transmembrane receptor protein tyrosine kinase inhibitor activity Source: MGI
GO - Biological processi
- adaptive immune response Source: UniProtKB-KW
- blood coagulation Source: MGI
- CD4-positive, alpha-beta T cell proliferation Source: MGI
- CD8-positive, alpha-beta T cell proliferation Source: MGI
- cellular response to lipopolysaccharide Source: MGI
- Fc receptor mediated inhibitory signaling pathway Source: MGI
- inflammatory response Source: MGI
- inflammatory response to antigenic stimulus Source: MGI
- negative regulation of activated T cell proliferation Source: MGI
- negative regulation of chemokine production Source: MGI
- negative regulation of cytokine production involved in inflammatory response Source: MGI
- negative regulation of cytotoxic T cell differentiation Source: MGI
- negative regulation of I-kappaB kinase/NF-kappaB signaling Source: MGI
- negative regulation of immunoglobulin production Source: UniProtKB
- negative regulation of interferon-gamma production Source: MGI
- negative regulation of interleukin-10 production Source: MGI
- negative regulation of interleukin-1 beta production Source: MGI
- negative regulation of interleukin-2 production Source: MGI
- negative regulation of interleukin-5 production Source: MGI
- negative regulation of interleukin-6 production Source: MGI
- negative regulation of IP-10 production Source: MGI
- negative regulation of MAPK cascade Source: MGI
- negative regulation of mast cell activation Source: UniProtKB
- negative regulation of mast cell activation involved in immune response Source: UniProtKB
- negative regulation of monocyte activation Source: UniProtKB
- negative regulation of natural killer cell activation Source: UniProtKB
- negative regulation of osteoclast differentiation Source: MGI
- negative regulation of plasma cell differentiation Source: UniProtKB
- negative regulation of pri-miRNA transcription by RNA polymerase II Source: MGI
- negative regulation of protein localization to nucleus Source: MGI
- negative regulation of protein tyrosine kinase activity Source: MGI
- negative regulation of signaling receptor activity Source: MGI
- negative regulation of T cell costimulation Source: MGI
- negative regulation of T cell cytokine production Source: MGI
- negative regulation of T cell proliferation Source: MGI
- negative regulation of T cell receptor signaling pathway Source: MGI
- neutrophil homeostasis Source: MGI
- positive regulation of CD8-positive, alpha-beta T cell differentiation Source: MGI
- positive regulation of regulatory T cell differentiation Source: MGI
- positive regulation of T cell anergy Source: MGI
- receptor internalization Source: MGI
- response to extracellular stimulus Source: MGI
- response to lipopolysaccharide Source: MGI
- response to nematode Source: MGI
- response to other organism Source: MGI
- response to wounding Source: MGI
- tolerance induction Source: MGI
- type 2 immune response Source: MGI
Keywordsi
Molecular function | Receptor |
Biological process | Adaptive immunity, Immunity |
Enzyme and pathway databases
Reactomei | R-MMU-6798695, Neutrophil degranulation |
Protein family/group databases
MEROPSi | I43.951 |
Names & Taxonomyi
Protein namesi | Recommended name: Leukocyte immunoglobulin-like receptor subfamily B member 4AImportedAlternative name(s): Mast cell surface glycoprotein Gp49B CD_antigen: CD85k |
Gene namesi | |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:102701, Lilrb4a |
VEuPathDBi | HostDB:ENSMUSG00000062593 HostDB:ENSMUSG00000112148 |
Subcellular locationi
Plasma membrane
- Cell membrane 4 Publications; Single-pass type I membrane protein Sequence analysis
Plasma Membrane
- external side of plasma membrane Source: MGI
- plasma membrane Source: GO_Central
Other locations
- cell surface Source: UniProtKB
- integral component of membrane Source: UniProtKB-KW
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 24 – 238 | ExtracellularSequence analysisAdd BLAST | 215 | |
Transmembranei | 239 – 260 | HelicalSequence analysisAdd BLAST | 22 | |
Topological domaini | 261 – 335 | CytoplasmicSequence analysisAdd BLAST | 75 |
Keywords - Cellular componenti
Cell membrane, MembranePathology & Biotechi
Disruption phenotypei
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 300 | Y → F: Partially suppresses inhibition of mast cell activation. Abolishes inhibition of mast cell activation; when associated with F-322. 1 Publication | 1 | |
Mutagenesisi | 322 | Y → F: Partially suppresses inhibition of mast cell activation. Abolishes inhibition of mast cell activation; when associated with F-300. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 23 | By similarityAdd BLAST | 23 | |
ChainiPRO_0000014824 | 24 – 335 | Leukocyte immunoglobulin-like receptor subfamily B member 4AAdd BLAST | 312 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Disulfide bondi | 49 ↔ 98 | PROSITE-ProRule annotation | ||
Glycosylationi | 133 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 144 ↔ 196 | PROSITE-ProRule annotation | ||
Glycosylationi | 191 | N-linked (GlcNAc...) asparagineSequence analysis | 1 |
Post-translational modificationi
Keywords - PTMi
Disulfide bond, Glycoprotein, PhosphoproteinProteomic databases
EPDi | Q64281 |
MaxQBi | Q64281 |
PaxDbi | Q64281 |
PeptideAtlasi | Q64281 |
PRIDEi | Q64281 |
ProteomicsDBi | 290039 [Q64281-2] |
PTM databases
GlyGeni | Q64281, 2 sites |
iPTMneti | Q64281 |
PhosphoSitePlusi | Q64281 |
Expressioni
Tissue specificityi
Inductioni
Gene expression databases
Bgeei | ENSMUSG00000062593, Expressed in granulocyte and 73 other tissues |
Genevisiblei | Q64281, MM |
Interactioni
Subunit structurei
Interacts (when tyrosine phosphorylated) with SH2 domain-containing phosphatases PTPN6/SHP-1 and PTPN11/SHP-2; interaction with PTPN6 enhances inhibition of mast cell activation.
2 PublicationsGO - Molecular functioni
- fibronectin binding Source: UniProtKB
- integrin binding Source: ARUK-UCL
- phosphatase binding Source: UniProtKB
- protein phosphatase binding Source: UniProtKB
Protein-protein interaction databases
BioGRIDi | 200008, 2 interactors |
DIPi | DIP-46122N |
IntActi | Q64281, 2 interactors |
STRINGi | 10090.ENSMUSP00000101121 |
Miscellaneous databases
RNActi | Q64281, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 42 – 125 | Ig-like C2-type 1Add BLAST | 84 | |
Domaini | 124 – 212 | Ig-like C2-type 2Add BLAST | 89 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 298 – 303 | ITIM motif 1 | 6 | |
Motifi | 320 – 325 | ITIM motif 2 | 6 |
Domaini
Keywords - Domaini
Immunoglobulin domain, Repeat, Signal, Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | ENOG502RU0A, Eukaryota |
GeneTreei | ENSGT01000000214458 |
HOGENOMi | CLU_021100_0_0_1 |
InParanoidi | Q64281 |
OMAi | EGRSEPW |
OrthoDBi | 1000446at2759 |
PhylomeDBi | Q64281 |
TreeFami | TF336644 |
Family and domain databases
Gene3Di | 2.60.40.10, 2 hits |
InterProi | View protein in InterPro IPR007110, Ig-like_dom IPR036179, Ig-like_dom_sf IPR013783, Ig-like_fold |
Pfami | View protein in Pfam PF13895, Ig_2, 2 hits |
SUPFAMi | SSF48726, SSF48726, 2 hits |
PROSITEi | View protein in PROSITE PS50835, IG_LIKE, 1 hit |
s (2+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 2 described isoforms and 4 potential isoforms that are computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MIAMLTVLLY LGLILEPRTA VQAGHLPKPI IWAEPGSVIA AYTSVITWCQ
60 70 80 90 100
GSWEAQYYHL YKEKSVNPWD TQVPLETRNK AKFNIPSMTT SYAGIYKCYY
110 120 130 140 150
ESAAGFSEHS DAMELVMTGA YENPSLSVYP SSNVTSGVSI SFSCSSSIVF
160 170 180 190 200
GRFILIQEGK HGLSWTLDSQ HQANQPSYAT FVLDAVTPNH NGTFRCYGYF
210 220 230 240 250
RNEPQVWSKP SNSLDLMISE TKDQSSTPTE DGLETYQKIL IGVLVSFLLL
260 270 280 290 300
FFLLLFLILI GYQYGHKKKA NASVKNTQSE NNAELNSWNP QNEDPQGIVY
310 320 330
AQVKPSRLQK DTACKETQDV TYAQLCIRTQ EQNNS
Computationally mapped potential isoform sequencesi
There are 4 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketA0A1W2P6F1 | A0A1W2P6F1_MOUSE | Leukocyte immunoglobulin-like recep... | Lilrb4a | 240 | Annotation score: | ||
A0A1W2P7X1 | A0A1W2P7X1_MOUSE | Predicted gene, 49339 | Gm49339 | 296 | Annotation score: | ||
A0A1W2P7B2 | A0A1W2P7B2_MOUSE | Leukocyte immunoglobulin-like recep... | Lilrb4a | 39 | Annotation score: | ||
A0A1W2P6K5 | A0A1W2P6K5_MOUSE | Leukocyte immunoglobulin-like recep... | Lilrb4a | 25 | Annotation score: |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_002510 | 232 | G → D in isoform 2. 1 Publication | 1 | |
Alternative sequenceiVSP_002511 | 233 – 271 | Missing in isoform 2. 1 PublicationAdd BLAST | 39 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U05266 mRNA Translation: AAA17799.1 U05265 Unassigned DNA Translation: AAA17797.1 U05265 Unassigned DNA Translation: AAA17798.1 U05264 mRNA Translation: AAA17796.1 BC006591 mRNA Translation: AAH06591.1 |
CCDSi | CCDS23832.1 [Q64281-1] |
PIRi | A53434 B53434 |
RefSeqi | NP_038560.1, NM_013532.3 [Q64281-1] |
Genome annotation databases
Ensembli | ENSMUST00000078778; ENSMUSP00000077833; ENSMUSG00000112148 [Q64281-1] ENSMUST00000217706; ENSMUSP00000151978; ENSMUSG00000062593 [Q64281-2] ENSMUST00000219696; ENSMUSP00000151486; ENSMUSG00000062593 [Q64281-1] ENSMUST00000220182; ENSMUSP00000151694; ENSMUSG00000112148 [Q64281-2] |
GeneIDi | 14728 |
KEGGi | mmu:14728 |
UCSCi | uc007far.2, mouse [Q64281-1] uc011xdq.2, mouse [Q64281-2] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U05266 mRNA Translation: AAA17799.1 U05265 Unassigned DNA Translation: AAA17797.1 U05265 Unassigned DNA Translation: AAA17798.1 U05264 mRNA Translation: AAA17796.1 BC006591 mRNA Translation: AAH06591.1 |
CCDSi | CCDS23832.1 [Q64281-1] |
PIRi | A53434 B53434 |
RefSeqi | NP_038560.1, NM_013532.3 [Q64281-1] |
3D structure databases
SMRi | Q64281 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 200008, 2 interactors |
DIPi | DIP-46122N |
IntActi | Q64281, 2 interactors |
STRINGi | 10090.ENSMUSP00000101121 |
Protein family/group databases
MEROPSi | I43.951 |
PTM databases
GlyGeni | Q64281, 2 sites |
iPTMneti | Q64281 |
PhosphoSitePlusi | Q64281 |
Proteomic databases
EPDi | Q64281 |
MaxQBi | Q64281 |
PaxDbi | Q64281 |
PeptideAtlasi | Q64281 |
PRIDEi | Q64281 |
ProteomicsDBi | 290039 [Q64281-2] |
Protocols and materials databases
DNASUi | 14728 |
Genome annotation databases
Ensembli | ENSMUST00000078778; ENSMUSP00000077833; ENSMUSG00000112148 [Q64281-1] ENSMUST00000217706; ENSMUSP00000151978; ENSMUSG00000062593 [Q64281-2] ENSMUST00000219696; ENSMUSP00000151486; ENSMUSG00000062593 [Q64281-1] ENSMUST00000220182; ENSMUSP00000151694; ENSMUSG00000112148 [Q64281-2] |
GeneIDi | 14728 |
KEGGi | mmu:14728 |
UCSCi | uc007far.2, mouse [Q64281-1] uc011xdq.2, mouse [Q64281-2] |
Organism-specific databases
CTDi | 14728 |
MGIi | MGI:102701, Lilrb4a |
VEuPathDBi | HostDB:ENSMUSG00000062593 HostDB:ENSMUSG00000112148 |
Phylogenomic databases
eggNOGi | ENOG502RU0A, Eukaryota |
GeneTreei | ENSGT01000000214458 |
HOGENOMi | CLU_021100_0_0_1 |
InParanoidi | Q64281 |
OMAi | EGRSEPW |
OrthoDBi | 1000446at2759 |
PhylomeDBi | Q64281 |
TreeFami | TF336644 |
Enzyme and pathway databases
Reactomei | R-MMU-6798695, Neutrophil degranulation |
Miscellaneous databases
BioGRID-ORCSi | 14728, 1 hit in 31 CRISPR screens |
PROi | PR:Q64281 |
RNActi | Q64281, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000062593, Expressed in granulocyte and 73 other tissues |
Genevisiblei | Q64281, MM |
Family and domain databases
Gene3Di | 2.60.40.10, 2 hits |
InterProi | View protein in InterPro IPR007110, Ig-like_dom IPR036179, Ig-like_dom_sf IPR013783, Ig-like_fold |
Pfami | View protein in Pfam PF13895, Ig_2, 2 hits |
SUPFAMi | SSF48726, SSF48726, 2 hits |
PROSITEi | View protein in PROSITE PS50835, IG_LIKE, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | LRB4A_MOUSE | |
Accessioni | Q64281Primary (citable) accession number: Q64281 Secondary accession number(s): Q64312 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 1, 1997 |
Last sequence update: | November 1, 1996 | |
Last modified: | February 23, 2022 | |
This is version 167 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot