UniProtKB - Q64280 (LFTY1_MOUSE)
Protein
Left-right determination factor 1
Gene
Lefty1
Organism
Mus musculus (Mouse)
Status
Functioni
Required for left-right axis determination as a regulator of LEFTY2 and NODAL.1 Publication
GO - Molecular functioni
- cytokine activity Source: GO_Central
- growth factor activity Source: UniProtKB-KW
- nodal binding Source: UniProtKB
- transforming growth factor beta receptor binding Source: InterPro
GO - Biological processi
- anterior/posterior axis specification Source: MGI
- BMP signaling pathway Source: GO_Central
- cell migration involved in gastrulation Source: MGI
- determination of left/right symmetry Source: BHF-UCL
- heart morphogenesis Source: BHF-UCL
- negative regulation of cell population proliferation Source: MGI
- negative regulation of nodal receptor complex assembly Source: UniProtKB
- negative regulation of nodal signaling pathway Source: UniProtKB
- negative regulation of transcription by RNA polymerase II Source: BHF-UCL
- positive regulation of pathway-restricted SMAD protein phosphorylation Source: GO_Central
- response to retinoic acid Source: MGI
- SMAD protein signal transduction Source: GO_Central
Keywordsi
Molecular function | Cytokine, Developmental protein, Growth factor |
Names & Taxonomyi
Protein namesi | Recommended name: Left-right determination factor 1Alternative name(s): Protein lefty-1 Short name: Lefty protein Stimulated by retinoic acid gene 3 protein Transforming growth factor beta-4 Short name: TGF-beta-4 |
Gene namesi | Name:Lefty1 Synonyms:Ebaf, Lefty, Stra3, Tgfb4 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:107405, Lefty1 |
Subcellular locationi
Extracellular region or secreted
Extracellular region or secreted
- extracellular region Source: UniProtKB
- extracellular space Source: GO_Central
Keywords - Cellular componenti
SecretedPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 21 | Sequence analysisAdd BLAST | 21 | |
PropeptideiPRO_0000033808 | 22 – 76 | Or 135Sequence analysisAdd BLAST | 55 | |
ChainiPRO_0000033809 | 77 – 368 | Left-right determination factor 1Add BLAST | 292 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Glycosylationi | 158 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 253 ↔ 266 | By similarity | ||
Disulfide bondi | 265 ↔ 318 | By similarity | ||
Disulfide bondi | 295 ↔ 353 | By similarity | ||
Disulfide bondi | 299 ↔ 355 | By similarity |
Post-translational modificationi
The processing of the protein may also occur at the second R-X-X-R site located at AA 132-135. Processing appears to be regulated in a cell-type specific manner.
Keywords - PTMi
Disulfide bond, GlycoproteinProteomic databases
PaxDbi | Q64280 |
PeptideAtlasi | Q64280 |
PRIDEi | Q64280 |
PTM databases
GlyGeni | Q64280, 1 site |
PhosphoSitePlusi | Q64280 |
Expressioni
Developmental stagei
By 8.0 dpc, expressed exclusively on the left side of developing embryos with expression predominantly in the prospective floor plate (PFP). Weak expression in the lateral-plate mesoderm (LPM).
Gene expression databases
Bgeei | ENSMUSG00000038793, Expressed in embryo and 134 other tissues |
Genevisiblei | Q64280, MM |
Interactioni
GO - Molecular functioni
- cytokine activity Source: GO_Central
- growth factor activity Source: UniProtKB-KW
- nodal binding Source: UniProtKB
- transforming growth factor beta receptor binding Source: InterPro
Protein-protein interaction databases
DIPi | DIP-46067N |
IntActi | Q64280, 1 interactor |
STRINGi | 10090.ENSMUSP00000041427 |
Miscellaneous databases
RNActi | Q64280, protein |
Family & Domainsi
Sequence similaritiesi
Belongs to the TGF-beta family.Curated
Keywords - Domaini
SignalPhylogenomic databases
eggNOGi | KOG3900, Eukaryota |
GeneTreei | ENSGT00390000010056 |
HOGENOMi | CLU_064098_0_0_1 |
InParanoidi | Q64280 |
OMAi | KDTCCRE |
OrthoDBi | 751948at2759 |
PhylomeDBi | Q64280 |
TreeFami | TF106462 |
Family and domain databases
Gene3Di | 2.10.90.10, 1 hit |
InterProi | View protein in InterPro IPR029034, Cystine-knot_cytokine IPR003942, LRDF IPR001839, TGF-b_C IPR001111, TGF-b_propeptide IPR015615, TGF-beta-rel IPR017948, TGFb_CS |
PANTHERi | PTHR11848, PTHR11848, 1 hit |
Pfami | View protein in Pfam PF00019, TGF_beta, 1 hit PF00688, TGFb_propeptide, 1 hit |
PIRSFi | PIRSF037402, TGFb4, 1 hit |
PRINTSi | PR01427, TGFBETA4 |
SMARTi | View protein in SMART SM00204, TGFB, 1 hit |
SUPFAMi | SSF57501, SSF57501, 1 hit |
PROSITEi | View protein in PROSITE PS00250, TGF_BETA_1, 1 hit PS51362, TGF_BETA_2, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
Q64280-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MPFLWLCWAL WALSLVSLRE ALTGEQILGS LLQQLQLDQP PVLDKADVEG
60 70 80 90 100
MVIPSHVRTQ YVALLQHSHA SRSRGKRFSQ NLREVAGRFL VSETSTHLLV
110 120 130 140 150
FGMEQRLPPN SELVQAVLRL FQEPVPRTAL RRQKRLSPHS ARARVTIEWL
160 170 180 190 200
RFRDDGSNRT ALIDSRLVSI HESGWKAFDV TEAVNFWQQL SRPRQPLLLQ
210 220 230 240 250
VSVQREHLGP GTWSSHKLVR FAAQGTPDGK GQGEPQLELH TLDLKDYGAQ
260 270 280 290 300
GNCDPEAPVT EGTRCCRQEM YLDLQGMKWA ENWILEPPGF LTYECVGSCL
310 320 330 340 350
QLPESLTSRW PFLGPRQCVA SEMTSLPMIV SVKEGGRTRP QVVSLPNMRV
360
QTCSCASDGA LIPRRLQP
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | D83921 mRNA Translation: BAA12121.1 Z73151 mRNA Translation: CAA97497.1 AJ000082 mRNA Translation: CAA03909.1 AJ000083 Genomic DNA Translation: CAA03910.1 AK131943 mRNA Translation: BAE20889.1 BC050221 mRNA Translation: AAH50221.1 |
CCDSi | CCDS15577.1 |
PIRi | S67507 |
RefSeqi | NP_034224.1, NM_010094.4 |
Genome annotation databases
Ensembli | ENSMUST00000037361; ENSMUSP00000041427; ENSMUSG00000038793 |
GeneIDi | 13590 |
KEGGi | mmu:13590 |
UCSCi | uc007dww.1, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | D83921 mRNA Translation: BAA12121.1 Z73151 mRNA Translation: CAA97497.1 AJ000082 mRNA Translation: CAA03909.1 AJ000083 Genomic DNA Translation: CAA03910.1 AK131943 mRNA Translation: BAE20889.1 BC050221 mRNA Translation: AAH50221.1 |
CCDSi | CCDS15577.1 |
PIRi | S67507 |
RefSeqi | NP_034224.1, NM_010094.4 |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
DIPi | DIP-46067N |
IntActi | Q64280, 1 interactor |
STRINGi | 10090.ENSMUSP00000041427 |
PTM databases
GlyGeni | Q64280, 1 site |
PhosphoSitePlusi | Q64280 |
Proteomic databases
PaxDbi | Q64280 |
PeptideAtlasi | Q64280 |
PRIDEi | Q64280 |
Genome annotation databases
Ensembli | ENSMUST00000037361; ENSMUSP00000041427; ENSMUSG00000038793 |
GeneIDi | 13590 |
KEGGi | mmu:13590 |
UCSCi | uc007dww.1, mouse |
Organism-specific databases
CTDi | 10637 |
MGIi | MGI:107405, Lefty1 |
Phylogenomic databases
eggNOGi | KOG3900, Eukaryota |
GeneTreei | ENSGT00390000010056 |
HOGENOMi | CLU_064098_0_0_1 |
InParanoidi | Q64280 |
OMAi | KDTCCRE |
OrthoDBi | 751948at2759 |
PhylomeDBi | Q64280 |
TreeFami | TF106462 |
Miscellaneous databases
BioGRID-ORCSi | 13590, 1 hit in 17 CRISPR screens |
PROi | PR:Q64280 |
RNActi | Q64280, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000038793, Expressed in embryo and 134 other tissues |
Genevisiblei | Q64280, MM |
Family and domain databases
Gene3Di | 2.10.90.10, 1 hit |
InterProi | View protein in InterPro IPR029034, Cystine-knot_cytokine IPR003942, LRDF IPR001839, TGF-b_C IPR001111, TGF-b_propeptide IPR015615, TGF-beta-rel IPR017948, TGFb_CS |
PANTHERi | PTHR11848, PTHR11848, 1 hit |
Pfami | View protein in Pfam PF00019, TGF_beta, 1 hit PF00688, TGFb_propeptide, 1 hit |
PIRSFi | PIRSF037402, TGFb4, 1 hit |
PRINTSi | PR01427, TGFBETA4 |
SMARTi | View protein in SMART SM00204, TGFB, 1 hit |
SUPFAMi | SSF57501, SSF57501, 1 hit |
PROSITEi | View protein in PROSITE PS00250, TGF_BETA_1, 1 hit PS51362, TGF_BETA_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | LFTY1_MOUSE | |
Accessioni | Q64280Primary (citable) accession number: Q64280 Secondary accession number(s): Q3V2A9 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 1, 1997 |
Last sequence update: | November 1, 1996 | |
Last modified: | December 2, 2020 | |
This is version 149 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families