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Protein

5-hydroxytryptamine receptor 1A

Gene

Htr1a

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

G-protein coupled receptor for 5-hydroxytryptamine (serotonin). Also functions as a receptor for various drugs and psychoactive substances. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors, such as adenylate cyclase. Beta-arrestin family members inhibit signaling via G proteins and mediate activation of alternative signaling pathways. Signaling inhibits adenylate cyclase activity and activates a phosphatidylinositol-calcium second messenger system that regulates the release of Ca2+ ions from intracellular stores. Plays a role in the regulation of 5-hydroxytryptamine release and in the regulation of dopamine and 5-hydroxytryptamine metabolism. Plays a role in the regulation of dopamine and 5-hydroxytryptamine levels in the brain, and thereby affects neural activity, mood and behavior. Plays a role in the response to anxiogenic stimuli.6 Publications

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionG-protein coupled receptor, Receptor, Transducer
Biological processBehavior

Enzyme and pathway databases

ReactomeiR-MMU-390666 Serotonin receptors
R-MMU-418594 G alpha (i) signalling events

Names & Taxonomyi

Protein namesi
Recommended name:
5-hydroxytryptamine receptor 1A
Short name:
5-HT-1A
Short name:
5-HT1A
Alternative name(s):
Serotonin receptor 1A
Gene namesi
Name:Htr1a
Synonyms:Gpcr18
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 13

Organism-specific databases

MGIiMGI:96273 Htr1a

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 36ExtracellularBy similarityAdd BLAST36
Transmembranei37 – 62Helical; Name=1By similarityAdd BLAST26
Topological domaini63 – 73CytoplasmicBy similarityAdd BLAST11
Transmembranei74 – 98Helical; Name=2By similarityAdd BLAST25
Topological domaini99 – 110ExtracellularBy similarityAdd BLAST12
Transmembranei111 – 132Helical; Name=3By similarityAdd BLAST22
Topological domaini133 – 152CytoplasmicBy similarityAdd BLAST20
Transmembranei153 – 178Helical; Name=4By similarityAdd BLAST26
Topological domaini179 – 191ExtracellularBy similarityAdd BLAST13
Transmembranei192 – 217Helical; Name=5By similarityAdd BLAST26
Topological domaini218 – 345CytoplasmicBy similarityAdd BLAST128
Transmembranei346 – 367Helical; Name=6By similarityAdd BLAST22
Topological domaini368 – 378ExtracellularBy similarityAdd BLAST11
Transmembranei379 – 403Helical; Name=7By similarityAdd BLAST25
Topological domaini404 – 421CytoplasmicBy similarityAdd BLAST18

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Disruption phenotypei

Mutant mice display decreased exploratory behavior and increased fear-related behavior in anxiogenic environments. Mutant mice display altered monoamine metabolism in specific parts of the brain, especially in dorsal and medial raphe nuclei, thalamus and hypothalamus, leading to altered levels of 5-hydroxytryptamine, dopamine and their metabolites, as well as altered noradrenaline levels.3 Publications

Chemistry databases

ChEMBLiCHEMBL3737

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000689041 – 4215-hydroxytryptamine receptor 1AAdd BLAST421

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi10N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi11N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi24N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi30N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi109 ↔ 187PROSITE-ProRule annotation

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

MaxQBiQ64264
PaxDbiQ64264
PRIDEiQ64264

PTM databases

iPTMnetiQ64264
PhosphoSitePlusiQ64264
SwissPalmiQ64264

Expressioni

Tissue specificityi

Most abundantly expressed in midbrain, in dorsal raphe and hippocampus. Detected at lower levels in amygdala and brain cortex.1 Publication

Gene expression databases

BgeeiENSMUSG00000021721 Expressed in 83 organ(s), highest expression level in CA1 field of hippocampus
CleanExiMM_HTR1A
GenevisibleiQ64264 MM

Interactioni

Subunit structurei

Heterodimer; heterodimerizes with GPER1.By similarity

GO - Molecular functioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000022235

Chemistry databases

BindingDBiQ64264

Structurei

3D structure databases

ProteinModelPortaliQ64264
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni112 – 121Agonist bindingBy similarity10
Regioni358 – 362Agonist bindingBy similarity5

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi133 – 135DRY motif; important for ligand-induced conformation changesBy similarity3
Motifi396 – 400NPxxY motif; important for ligand-induced conformation changes and signalingBy similarity5

Sequence similaritiesi

Belongs to the G-protein coupled receptor 1 family. 5-hydroxytryptamine receptor subfamily. HTR1A sub-subfamily.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3656 Eukaryota
ENOG410XRW9 LUCA
GeneTreeiENSGT00760000118795
HOGENOMiHOG000239242
HOVERGENiHBG106962
InParanoidiQ64264
KOiK04153
OMAiYQVLNKW
OrthoDBiEOG091G06VI
TreeFamiTF316350

Family and domain databases

CDDicd15330 7tmA_5-HT1A_vertebrates, 1 hit
InterProiView protein in InterPro
IPR000610 5HT1A_rcpt
IPR002231 5HT_rcpt
IPR000276 GPCR_Rhodpsn
IPR017452 GPCR_Rhodpsn_7TM
PANTHERiPTHR24247:SF20 PTHR24247:SF20, 1 hit
PfamiView protein in Pfam
PF00001 7tm_1, 1 hit
PRINTSiPR00512 5HT1ARECEPTR
PR01101 5HTRECEPTOR
PR00237 GPCRRHODOPSN
SMARTiView protein in SMART
SM01381 7TM_GPCR_Srsx, 1 hit
PROSITEiView protein in PROSITE
PS00237 G_PROTEIN_RECEP_F1_1, 1 hit
PS50262 G_PROTEIN_RECEP_F1_2, 1 hit

Sequencei

Sequence statusi: Complete.

Q64264-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MDMFSLGQGN NTTTSLEPFG TGGNDTGLSN VTFSYQVITS LLLGTLIFCA
60 70 80 90 100
VLGNACVVAA IALERSLQNV ANYLIGSLAV TDLMVSVLVL PMAALYQVLN
110 120 130 140 150
KWTLGQVTCD LFIALDVLCC TSSILHLCAI ALDRYWAITD PIDYVNKRTP
160 170 180 190 200
RRAAALISLT WLIGFLISIP PMLGWRTPED RSNPNECTIS KDHGYTIYST
210 220 230 240 250
FGAFYIPLLL MLVLYGRIFR AARFRIRKTV KKVEKKGAGT SFGTSSAPPP
260 270 280 290 300
KKSLNGQPGS GDCRRSAENR AVGTPCANGA VRQGEDDATL EVIEVHRVGN
310 320 330 340 350
SKGHLPLPSE SGATSYVPAC LERKNERTAE AKRKMALARE RKTVKTLGII
360 370 380 390 400
MGTFILCWLP FFIVALVLPF CESSCHMPEL LGAIINWLGY SNSLLNPVIY
410 420
AYFNKDFQNA FKKIIKCKFC R
Length:421
Mass (Da):46,176
Last modified:July 27, 2011 - v2
Checksum:i74F519DF7CC0C7AA
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti177T → A in AAA81519 (PubMed:8254366).Curated1
Sequence conflicti242F → L in AAA16850 (PubMed:8288218).Curated1
Sequence conflicti247A → V in AAA16850 (PubMed:8288218).Curated1
Sequence conflicti263C → W in AAA16850 (PubMed:8288218).Curated1
Sequence conflicti304H → D in AAA81519 (PubMed:8254366).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti17 – 19EPF → DHL in strain: C3H/An. 3

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U39391 mRNA Translation: AAA81519.1
AK043668 mRNA Translation: BAC31611.1
AK049814 mRNA Translation: BAC33931.1
AK049884 mRNA Translation: BAC33970.1
AK140194 mRNA Translation: BAE24273.1
CH466568 Genomic DNA Translation: EDL18479.1
BC138669 mRNA Translation: AAI38670.1
BC138681 mRNA Translation: AAI38682.1
U33820 Genomic DNA Translation: AAC52572.1
L20339 mRNA Translation: AAA16850.1
CCDSiCCDS26756.1
PIRiI49375
RefSeqiNP_032334.2, NM_008308.4
UniGeneiMm.4716

Genome annotation databases

EnsembliENSMUST00000022235; ENSMUSP00000022235; ENSMUSG00000021721
GeneIDi15550
KEGGimmu:15550
UCSCiuc007rtu.2 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U39391 mRNA Translation: AAA81519.1
AK043668 mRNA Translation: BAC31611.1
AK049814 mRNA Translation: BAC33931.1
AK049884 mRNA Translation: BAC33970.1
AK140194 mRNA Translation: BAE24273.1
CH466568 Genomic DNA Translation: EDL18479.1
BC138669 mRNA Translation: AAI38670.1
BC138681 mRNA Translation: AAI38682.1
U33820 Genomic DNA Translation: AAC52572.1
L20339 mRNA Translation: AAA16850.1
CCDSiCCDS26756.1
PIRiI49375
RefSeqiNP_032334.2, NM_008308.4
UniGeneiMm.4716

3D structure databases

ProteinModelPortaliQ64264
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000022235

Chemistry databases

BindingDBiQ64264
ChEMBLiCHEMBL3737

Protein family/group databases

GPCRDBiSearch...

PTM databases

iPTMnetiQ64264
PhosphoSitePlusiQ64264
SwissPalmiQ64264

Proteomic databases

MaxQBiQ64264
PaxDbiQ64264
PRIDEiQ64264

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000022235; ENSMUSP00000022235; ENSMUSG00000021721
GeneIDi15550
KEGGimmu:15550
UCSCiuc007rtu.2 mouse

Organism-specific databases

CTDi3350
MGIiMGI:96273 Htr1a

Phylogenomic databases

eggNOGiKOG3656 Eukaryota
ENOG410XRW9 LUCA
GeneTreeiENSGT00760000118795
HOGENOMiHOG000239242
HOVERGENiHBG106962
InParanoidiQ64264
KOiK04153
OMAiYQVLNKW
OrthoDBiEOG091G06VI
TreeFamiTF316350

Enzyme and pathway databases

ReactomeiR-MMU-390666 Serotonin receptors
R-MMU-418594 G alpha (i) signalling events

Miscellaneous databases

PROiPR:Q64264
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000021721 Expressed in 83 organ(s), highest expression level in CA1 field of hippocampus
CleanExiMM_HTR1A
GenevisibleiQ64264 MM

Family and domain databases

CDDicd15330 7tmA_5-HT1A_vertebrates, 1 hit
InterProiView protein in InterPro
IPR000610 5HT1A_rcpt
IPR002231 5HT_rcpt
IPR000276 GPCR_Rhodpsn
IPR017452 GPCR_Rhodpsn_7TM
PANTHERiPTHR24247:SF20 PTHR24247:SF20, 1 hit
PfamiView protein in Pfam
PF00001 7tm_1, 1 hit
PRINTSiPR00512 5HT1ARECEPTR
PR01101 5HTRECEPTOR
PR00237 GPCRRHODOPSN
SMARTiView protein in SMART
SM01381 7TM_GPCR_Srsx, 1 hit
PROSITEiView protein in PROSITE
PS00237 G_PROTEIN_RECEP_F1_1, 1 hit
PS50262 G_PROTEIN_RECEP_F1_2, 1 hit
ProtoNetiSearch...

Entry informationi

Entry namei5HT1A_MOUSE
AccessioniPrimary (citable) accession number: Q64264
Secondary accession number(s): Q60956, Q61617, Q8BGS4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: July 27, 2011
Last modified: November 7, 2018
This is version 158 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. 7-transmembrane G-linked receptors
    List of 7-transmembrane G-linked receptor entries
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Main funding by: National Institutes of Health

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