Htr1a

Functionⁱ

G-protein coupled receptor for 5-hydroxytryptamine (serotonin). Also functions as a receptor for various drugs and psychoactive substances. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors, such as adenylate cyclase. Beta-arrestin family members inhibit signaling via G proteins and mediate activation of alternative signaling pathways. Signaling inhibits adenylate cyclase activity and activates a phosphatidylinositol-calcium second messenger system that regulates the release of Ca²⁺ ions from intracellular stores. Plays a role in the regulation of 5-hydroxytryptamine release and in the regulation of dopamine and 5-hydroxytryptamine metabolism. Plays a role in the regulation of dopamine and 5-hydroxytryptamine levels in the brain, and thereby affects neural activity, mood and behavior. Plays a role in the response to anxiogenic stimuli.6 Publications

GO - Molecular functionⁱ

drug binding Source: MGI
G-protein alpha-subunit binding Source: MGI
G protein-coupled receptor activity
Source: GO_Central
Inferred from biological aspect of ancestorⁱ
- 21873635
G protein-coupled serotonin receptor activity
Source: MGI
Inferred from direct assayⁱ
- 8254366
neurotransmitter receptor activity
Source: GO_Central
Inferred from biological aspect of ancestorⁱ
- 21873635
receptor-receptor interaction Source: MGI
serotonin binding Source: MGI
signaling receptor binding Source: MGI

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

adenylate cyclase-inhibiting serotonin receptor signaling pathway Source: UniProtKB
behavioral fear response
Source: UniProtKB
Inferred from mutant phenotypeⁱ
- 9826725
cell proliferation Source: InterPro
chemical synaptic transmission
Source: GO_Central
Inferred from biological aspect of ancestorⁱ
- 21873635
exploration behavior
Source: UniProtKB
Inferred from mutant phenotypeⁱ
- 9826725
G protein-coupled receptor signaling pathway Source: MGI
G protein-coupled receptor signaling pathway, coupled to cyclic nucleotide second messenger
Source: GO_Central
Inferred from biological aspect of ancestorⁱ
- 21873635
NMDA glutamate receptor clustering Source: MGI
positive regulation of microtubule depolymerization Source: MGI
regulation of dopamine metabolic process
Source: UniProtKB
Inferred from mutant phenotypeⁱ
- 11080193
regulation of feeding behavior Source: MGI
regulation of hormone secretion Source: InterPro
regulation of serotonin secretion
Source: UniProtKB
Inferred from mutant phenotypeⁱ
- 9826725
regulation of synaptic vesicle exocytosis Source: MGI
serotonin metabolic process
Source: UniProtKB
Inferred from mutant phenotypeⁱ
- 11080193
serotonin receptor signaling pathway
Source: UniProtKB
Inferred from mutant phenotypeⁱ
- 9826725
vasoconstriction Source: InterPro

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Molecular function	G-protein coupled receptor, Receptor, Transducer
Biological process	Behavior

Enzyme and pathway databases

Reactomeⁱ	R-MMU-390666 Serotonin receptors R-MMU-418594 G alpha (i) signalling events

Reactomeⁱ

R-MMU-390666 Serotonin receptors
R-MMU-418594 G alpha (i) signalling events

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: 5-hydroxytryptamine receptor 1A Short name: 5-HT-1A Short name: 5-HT1A Alternative name(s): Serotonin receptor 1A
Gene namesⁱ	Name:Htr1a Synonyms:Gpcr18
Organismⁱ	Mus musculus (Mouse)
Taxonomic identifierⁱ	10090 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Dipnotetrapodomorpha › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Boreoeutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus
Proteomesⁱ	UP000000589 Componentⁱ: Chromosome 13

Organism-specific databases

MGIⁱ	MGI:96273 Htr1a

Topology

Feature key	Position(s)	DescriptionActions	Length
Topological domainⁱ	1 – 36	ExtracellularBy similarityAdd BLAST	36
Transmembraneⁱ	37 – 62	Helical; Name=1By similarityAdd BLAST	26
Topological domainⁱ	63 – 73	CytoplasmicBy similarityAdd BLAST	11
Transmembraneⁱ	74 – 98	Helical; Name=2By similarityAdd BLAST	25
Topological domainⁱ	99 – 110	ExtracellularBy similarityAdd BLAST	12
Transmembraneⁱ	111 – 132	Helical; Name=3By similarityAdd BLAST	22
Topological domainⁱ	133 – 152	CytoplasmicBy similarityAdd BLAST	20
Transmembraneⁱ	153 – 178	Helical; Name=4By similarityAdd BLAST	26
Topological domainⁱ	179 – 191	ExtracellularBy similarityAdd BLAST	13
Transmembraneⁱ	192 – 217	Helical; Name=5By similarityAdd BLAST	26
Topological domainⁱ	218 – 345	CytoplasmicBy similarityAdd BLAST	128
Transmembraneⁱ	346 – 367	Helical; Name=6By similarityAdd BLAST	22
Topological domainⁱ	368 – 378	ExtracellularBy similarityAdd BLAST	11
Transmembraneⁱ	379 – 403	Helical; Name=7By similarityAdd BLAST	25
Topological domainⁱ	404 – 421	CytoplasmicBy similarityAdd BLAST	18

Keywords - Cellular componentⁱ

Cell membrane, Membrane

Pathology & Biotechⁱ

Disruption phenotypeⁱ

Mutant mice display decreased exploratory behavior and increased fear-related behavior in anxiogenic environments. Mutant mice display altered monoamine metabolism in specific parts of the brain, especially in dorsal and medial raphe nuclei, thalamus and hypothalamus, leading to altered levels of 5-hydroxytryptamine, dopamine and their metabolites, as well as altered noradrenaline levels.3 Publications

Chemistry databases

ChEMBLⁱ	CHEMBL3737

ChEMBLⁱ

CHEMBL3737

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
ChainⁱPRO_0000068904	1 – 421	5-hydroxytryptamine receptor 1AAdd BLAST		421

Amino acid modifications

Feature key	Position(s)	DescriptionActions	Length
Glycosylationⁱ	10	N-linked (GlcNAc...) asparagineSequence analysis	1
Glycosylationⁱ	11	N-linked (GlcNAc...) asparagineSequence analysis	1
Glycosylationⁱ	24	N-linked (GlcNAc...) asparagineSequence analysis	1
Glycosylationⁱ	30	N-linked (GlcNAc...) asparagineSequence analysis	1
Disulfide bondⁱ	109 ↔ 187	PROSITE-ProRule annotation

Keywords - PTMⁱ

Disulfide bond, Glycoprotein

Proteomic databases

MaxQB - The MaxQuant DataBase More...MaxQBⁱ	Q64264
PaxDbⁱ	Q64264
PRIDEⁱ	Q64264

PTM databases

iPTMnetⁱ	Q64264
PhosphoSitePlusⁱ	Q64264
SwissPalmⁱ	Q64264

Expressionⁱ

Tissue specificityⁱ

Most abundantly expressed in midbrain, in dorsal raphe and hippocampus. Detected at lower levels in amygdala and brain cortex.1 Publication

Gene expression databases

Bgeeⁱ	ENSMUSG00000021721 Expressed in 83 organ(s), highest expression level in CA1 field of hippocampus
CleanExⁱ	MM_HTR1A
Genevisibleⁱ	Q64264 MM

Interactionⁱ

Subunit structureⁱ

Heterodimer; heterodimerizes with GPER1.By similarity

GO - Molecular functionⁱ

G-protein alpha-subunit binding Source: MGI
receptor-receptor interaction Source: MGI
signaling receptor binding Source: MGI

View the complete GO annotation on QuickGO ...

Protein-protein interaction databases

STRINGⁱ	10090.ENSMUSP00000022235

STRINGⁱ

10090.ENSMUSP00000022235

Chemistry databases

BindingDBⁱ

Q64264

Structureⁱ

3D structure databases

ProteinModelPortalⁱ	Q64264
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Family & Domainsⁱ

Region

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Regionⁱ	112 – 121	Agonist bindingBy similarity		10
Regionⁱ	358 – 362	Agonist bindingBy similarity		5

Motif

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Motifⁱ	133 – 135	DRY motif; important for ligand-induced conformation changesBy similarity		3
Motifⁱ	396 – 400	NPxxY motif; important for ligand-induced conformation changes and signalingBy similarity		5

Sequence similaritiesⁱ

Belongs to the G-protein coupled receptor 1 family. 5-hydroxytryptamine receptor subfamily. HTR1A sub-subfamily.PROSITE-ProRule annotation

Keywords - Domainⁱ

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGⁱ	KOG3656 Eukaryota ENOG410XRW9 LUCA
Ensembl GeneTree More...GeneTreeⁱ	ENSGT00760000118795
HOGENOMⁱ	HOG000239242
HOVERGENⁱ	HBG106962
InParanoidⁱ	Q64264
KEGG Orthology (KO) More...KOⁱ	K04153
OMAⁱ	YQVLNKW
Database of Orthologous Groups More...OrthoDBⁱ	EOG091G06VI
TreeFamⁱ	TF316350

Family and domain databases

Conserved Domains Database More...CDDⁱ	cd15330 7tmA_5-HT1A_vertebrates, 1 hit
InterProⁱ	View protein in InterPro IPR000610 5HT1A_rcpt IPR002231 5HT_rcpt IPR000276 GPCR_Rhodpsn IPR017452 GPCR_Rhodpsn_7TM
PANTHERⁱ	PTHR24247:SF20 PTHR24247:SF20, 1 hit
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF00001 7tm_1, 1 hit
PRINTSⁱ	PR00512 5HT1ARECEPTR PR01101 5HTRECEPTOR PR00237 GPCRRHODOPSN
SMARTⁱ	View protein in SMART SM01381 7TM_GPCR_Srsx, 1 hit
PROSITEⁱ	View protein in PROSITE PS00237 G_PROTEIN_RECEP_F1_1, 1 hit PS50262 G_PROTEIN_RECEP_F1_2, 1 hit

Sequenceⁱ

Sequence statusⁱ: Complete.

Q64264-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
MDMFSLGQGN NTTTSLEPFG TGGNDTGLSN VTFSYQVITS LLLGTLIFCA 
        60         70         80         90        100
VLGNACVVAA IALERSLQNV ANYLIGSLAV TDLMVSVLVL PMAALYQVLN 
       110        120        130        140        150
KWTLGQVTCD LFIALDVLCC TSSILHLCAI ALDRYWAITD PIDYVNKRTP 
       160        170        180        190        200
RRAAALISLT WLIGFLISIP PMLGWRTPED RSNPNECTIS KDHGYTIYST 
       210        220        230        240        250
FGAFYIPLLL MLVLYGRIFR AARFRIRKTV KKVEKKGAGT SFGTSSAPPP 
       260        270        280        290        300
KKSLNGQPGS GDCRRSAENR AVGTPCANGA VRQGEDDATL EVIEVHRVGN 
       310        320        330        340        350
SKGHLPLPSE SGATSYVPAC LERKNERTAE AKRKMALARE RKTVKTLGII 
       360        370        380        390        400
MGTFILCWLP FFIVALVLPF CESSCHMPEL LGAIINWLGY SNSLLNPVIY 
       410        420 
AYFNKDFQNA FKKIIKCKFC R

Length:421

Mass (Da):46,176

Last modified:July 27, 2011 - v2

Checksum:ⁱ74F519DF7CC0C7AA

GO

Experimental Info

Feature key	Position(s)	DescriptionActions	Length
Sequence conflictⁱ	177	T → A in AAA81519 (PubMed:8254366).Curated	1
Sequence conflictⁱ	242	F → L in AAA16850 (PubMed:8288218).Curated	1
Sequence conflictⁱ	247	A → V in AAA16850 (PubMed:8288218).Curated	1
Sequence conflictⁱ	263	C → W in AAA16850 (PubMed:8288218).Curated	1
Sequence conflictⁱ	304	H → D in AAA81519 (PubMed:8254366).Curated	1

Natural variant

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Natural variantⁱ	17 – 19	EPF → DHL in strain: C3H/An.		3

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	U39391 mRNA Translation: AAA81519.1 AK043668 mRNA Translation: BAC31611.1 AK049814 mRNA Translation: BAC33931.1 AK049884 mRNA Translation: BAC33970.1 AK140194 mRNA Translation: BAE24273.1 CH466568 Genomic DNA Translation: EDL18479.1 BC138669 mRNA Translation: AAI38670.1 BC138681 mRNA Translation: AAI38682.1 U33820 Genomic DNA Translation: AAC52572.1 L20339 mRNA Translation: AAA16850.1
The Consensus CDS (CCDS) project More...CCDSⁱ	CCDS26756.1
PIRⁱ	I49375
NCBI Reference Sequences More...RefSeqⁱ	NP_032334.2, NM_008308.4
UniGeneⁱ	Mm.4716

Genome annotation databases

Ensemblⁱ	ENSMUST00000022235; ENSMUSP00000022235; ENSMUSG00000021721
GeneIDⁱ	15550
KEGGⁱ	mmu:15550
UCSC genome browser More...UCSCⁱ	uc007rtu.2 mouse

Similar proteinsⁱ

90% Identity
50% Identity

Protein	Similar proteins		Species	Score	Length	Source
Q64264	5-hydroxytryptamine receptor 1A		RAT		422	UniRef90_P19327
Serotonin receptor 1A (Fragment)		CAVPO		143
Uncharacterized protein		NEOLE		443
5-hydroxytryptamine receptor		CRIGR		422
5-hydroxytryptamine receptor 1A		MESAU		422
+11

Protein	Similar proteins		Species	Score	Length	Source
Q64264	5-hydroxytryptamine receptor 1A		RAT		422	UniRef50_P19327
Serotonin receptor 1A (Fragment)		CAVPO		143
Uncharacterized protein		NEOLE		443
5-hydroxytryptamine receptor		CRIGR		422
5-hydroxytryptamine receptor 1A		MESAU		422
+89

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	U39391 mRNA Translation: AAA81519.1 AK043668 mRNA Translation: BAC31611.1 AK049814 mRNA Translation: BAC33931.1 AK049884 mRNA Translation: BAC33970.1 AK140194 mRNA Translation: BAE24273.1 CH466568 Genomic DNA Translation: EDL18479.1 BC138669 mRNA Translation: AAI38670.1 BC138681 mRNA Translation: AAI38682.1 U33820 Genomic DNA Translation: AAC52572.1 L20339 mRNA Translation: AAA16850.1
CCDSⁱ	CCDS26756.1
PIRⁱ	I49375
RefSeqⁱ	NP_032334.2, NM_008308.4
UniGeneⁱ	Mm.4716

3D structure databases

ProteinModelPortalⁱ	Q64264
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

STRINGⁱ	10090.ENSMUSP00000022235

STRINGⁱ

10090.ENSMUSP00000022235

Chemistry databases

BindingDBⁱ	Q64264
ChEMBLⁱ	CHEMBL3737

Protein family/group databases

GPCRDBⁱ	Search...

GPCRDBⁱ

Search...

PTM databases

iPTMnetⁱ	Q64264
PhosphoSitePlusⁱ	Q64264
SwissPalmⁱ	Q64264

Proteomic databases

MaxQBⁱ	Q64264
PaxDbⁱ	Q64264
PRIDEⁱ	Q64264

Protocols and materials databases

Structural Biology Knowledgebase	Search...

Structural Biology Knowledgebase

Search...

Genome annotation databases

Ensemblⁱ	ENSMUST00000022235; ENSMUSP00000022235; ENSMUSG00000021721
GeneIDⁱ	15550
KEGGⁱ	mmu:15550
UCSCⁱ	uc007rtu.2 mouse

Organism-specific databases

CTDⁱ	3350
MGIⁱ	MGI:96273 Htr1a

Phylogenomic databases

eggNOGⁱ	KOG3656 Eukaryota ENOG410XRW9 LUCA
GeneTreeⁱ	ENSGT00760000118795
HOGENOMⁱ	HOG000239242
HOVERGENⁱ	HBG106962
InParanoidⁱ	Q64264
KOⁱ	K04153
OMAⁱ	YQVLNKW
OrthoDBⁱ	EOG091G06VI
TreeFamⁱ	TF316350

Enzyme and pathway databases

Reactomeⁱ	R-MMU-390666 Serotonin receptors R-MMU-418594 G alpha (i) signalling events

Reactomeⁱ

R-MMU-390666 Serotonin receptors
R-MMU-418594 G alpha (i) signalling events

Miscellaneous databases

Protein Ontology More...PROⁱ	PR:Q64264
SOURCEⁱ	Search...

Gene expression databases

Bgeeⁱ	ENSMUSG00000021721 Expressed in 83 organ(s), highest expression level in CA1 field of hippocampus
CleanExⁱ	MM_HTR1A
Genevisibleⁱ	Q64264 MM

Family and domain databases

CDDⁱ	cd15330 7tmA_5-HT1A_vertebrates, 1 hit
InterProⁱ	View protein in InterPro IPR000610 5HT1A_rcpt IPR002231 5HT_rcpt IPR000276 GPCR_Rhodpsn IPR017452 GPCR_Rhodpsn_7TM
PANTHERⁱ	PTHR24247:SF20 PTHR24247:SF20, 1 hit
Pfamⁱ	View protein in Pfam PF00001 7tm_1, 1 hit
PRINTSⁱ	PR00512 5HT1ARECEPTR PR01101 5HTRECEPTOR PR00237 GPCRRHODOPSN
SMARTⁱ	View protein in SMART SM01381 7TM_GPCR_Srsx, 1 hit
PROSITEⁱ	View protein in PROSITE PS00237 G_PROTEIN_RECEP_F1_1, 1 hit PS50262 G_PROTEIN_RECEP_F1_2, 1 hit
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	5HT1A_MOUSE
Accessionⁱ	Q64264Primary (citable) accession number: Q64264 Secondary accession number(s): Q60956, Q61617, Q8BGS4
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
	Last sequence update:	July 27, 2011
	Last modified:	November 7, 2018
	This is version 158 of the entry and version 2 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Chordata Protein Annotation Program

Miscellaneousⁱ

Keywords - Technical termⁱ

Complete proteome, Reference proteome

Documents

SIMILARITY comments
Index of protein domains and families
MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
7-transmembrane G-linked receptors
List of 7-transmembrane G-linked receptor entries

UniProtKB

UniParc

Help

UniRef

Proteomes

Annotation systems

Supporting data

UniProtKB - Q64264 (5HT1A_MOUSE)

Your basket is currently empty. i <p>When browsing through different UniProt proteins, you can use the ‘basket’ to save them, so that you can back to find or analyse them later.<p><a href='/help/basket' target='_top'>More...</a></p>

5-hydroxytryptamine receptor 1A

Select a section on the left to see content.

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

Enzyme and pathway databases

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

Organism-specific databases

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Plasma membrane

Plasma Membrane

Other locations

Topology

Keywords - Cellular componenti

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

Chemistry databases

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Amino acid modifications

Keywords - PTMi

Proteomic databases

PTM databases

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

Gene expression databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

GO - Molecular functioni

Protein-protein interaction databases

Chemistry databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Region

Motif

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Keywords - Domaini

Phylogenomic databases

Family and domain databases

Experimental Info

Natural variant

Sequence databases

Genome annotation databases

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protein-protein interaction databases

Chemistry databases

Protein family/group databases

PTM databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Miscellaneous databases

Gene expression databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

Documents

UniProt is an ELIXIR core data resource

Your basket is currently empty. ⁱ

Functionⁱ

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

Keywords - Cellular componentⁱ

Pathology & Biotechⁱ

PTM / Processingⁱ

Keywords - PTMⁱ

Expressionⁱ

Interactionⁱ

GO - Molecular functionⁱ

Structureⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Keywords - Domainⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ

Keywords - Technical termⁱ