UniProtKB - Q64264 (5HT1A_MOUSE)
Protein
5-hydroxytryptamine receptor 1A
Gene
Htr1a
Organism
Mus musculus (Mouse)
Status
Functioni
G-protein coupled receptor for 5-hydroxytryptamine (serotonin). Also functions as a receptor for various drugs and psychoactive substances. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors, such as adenylate cyclase. Beta-arrestin family members inhibit signaling via G proteins and mediate activation of alternative signaling pathways. Signaling inhibits adenylate cyclase activity and activates a phosphatidylinositol-calcium second messenger system that regulates the release of Ca2+ ions from intracellular stores. Plays a role in the regulation of 5-hydroxytryptamine release and in the regulation of dopamine and 5-hydroxytryptamine metabolism. Plays a role in the regulation of dopamine and 5-hydroxytryptamine levels in the brain, and thereby affects neural activity, mood and behavior. Plays a role in the response to anxiogenic stimuli.6 Publications
GO - Molecular functioni
- G-protein alpha-subunit binding Source: MGI
- G protein-coupled serotonin receptor activity Source: MGI
- neurotransmitter receptor activity Source: GO_Central
- receptor-receptor interaction Source: MGI
- serotonin binding Source: MGI
- signaling receptor binding Source: MGI
GO - Biological processi
- adenylate cyclase-inhibiting serotonin receptor signaling pathway Source: UniProtKB
- behavioral fear response Source: UniProtKB
- chemical synaptic transmission Source: GO_Central
- exploration behavior Source: UniProtKB
- gamma-aminobutyric acid signaling pathway Source: MGI
- G protein-coupled receptor signaling pathway Source: MGI
- G protein-coupled receptor signaling pathway, coupled to cyclic nucleotide second messenger Source: GO_Central
- negative regulation of gamma-aminobutyric acid secretion Source: MGI
- NMDA glutamate receptor clustering Source: MGI
- positive regulation of microtubule depolymerization Source: MGI
- regulation of dopamine metabolic process Source: UniProtKB
- regulation of feeding behavior Source: MGI
- regulation of hormone secretion Source: InterPro
- regulation of serotonin secretion Source: UniProtKB
- regulation of synaptic vesicle exocytosis Source: MGI
- regulation of vasoconstriction Source: InterPro
- serotonin metabolic process Source: UniProtKB
- serotonin receptor signaling pathway Source: UniProtKB
Keywordsi
Molecular function | G-protein coupled receptor, Receptor, Transducer |
Biological process | Behavior |
Enzyme and pathway databases
Reactomei | R-MMU-390666, Serotonin receptors |
Names & Taxonomyi
Protein namesi | Recommended name: 5-hydroxytryptamine receptor 1AShort name: 5-HT-1A Short name: 5-HT1A Alternative name(s): Serotonin receptor 1A |
Gene namesi | Name:Htr1a Synonyms:Gpcr18 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:96273, Htr1a |
Subcellular locationi
Plasma membrane
- Cell membrane 1 Publication; Multi-pass membrane protein 1 Publication
Other locations
- dendrite By similarity
Plasma Membrane
- integral component of plasma membrane Source: UniProtKB
- integral component of presynaptic membrane Source: MGI
Other locations
- axon hillock Source: MGI
- dendrite Source: GO_Central
- GABA-ergic synapse Source: MGI
- neuronal cell body Source: MGI
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 36 | ExtracellularBy similarityAdd BLAST | 36 | |
Transmembranei | 37 – 62 | Helical; Name=1By similarityAdd BLAST | 26 | |
Topological domaini | 63 – 73 | CytoplasmicBy similarityAdd BLAST | 11 | |
Transmembranei | 74 – 98 | Helical; Name=2By similarityAdd BLAST | 25 | |
Topological domaini | 99 – 110 | ExtracellularBy similarityAdd BLAST | 12 | |
Transmembranei | 111 – 132 | Helical; Name=3By similarityAdd BLAST | 22 | |
Topological domaini | 133 – 152 | CytoplasmicBy similarityAdd BLAST | 20 | |
Transmembranei | 153 – 178 | Helical; Name=4By similarityAdd BLAST | 26 | |
Topological domaini | 179 – 191 | ExtracellularBy similarityAdd BLAST | 13 | |
Transmembranei | 192 – 217 | Helical; Name=5By similarityAdd BLAST | 26 | |
Topological domaini | 218 – 345 | CytoplasmicBy similarityAdd BLAST | 128 | |
Transmembranei | 346 – 367 | Helical; Name=6By similarityAdd BLAST | 22 | |
Topological domaini | 368 – 378 | ExtracellularBy similarityAdd BLAST | 11 | |
Transmembranei | 379 – 403 | Helical; Name=7By similarityAdd BLAST | 25 | |
Topological domaini | 404 – 421 | CytoplasmicBy similarityAdd BLAST | 18 |
Keywords - Cellular componenti
Cell membrane, Cell projection, MembranePathology & Biotechi
Disruption phenotypei
Mutant mice display decreased exploratory behavior and increased fear-related behavior in anxiogenic environments. Mutant mice display altered monoamine metabolism in specific parts of the brain, especially in dorsal and medial raphe nuclei, thalamus and hypothalamus, leading to altered levels of 5-hydroxytryptamine, dopamine and their metabolites, as well as altered noradrenaline levels.3 Publications
Chemistry databases
ChEMBLi | CHEMBL3737 |
DrugCentrali | Q64264 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000068904 | 1 – 421 | 5-hydroxytryptamine receptor 1AAdd BLAST | 421 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Glycosylationi | 10 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 11 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 24 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 30 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 109 ↔ 187 | PROSITE-ProRule annotation |
Keywords - PTMi
Disulfide bond, GlycoproteinProteomic databases
MaxQBi | Q64264 |
PaxDbi | Q64264 |
PRIDEi | Q64264 |
ProteomicsDBi | 285688 |
PTM databases
GlyGeni | Q64264, 4 sites |
iPTMneti | Q64264 |
PhosphoSitePlusi | Q64264 |
SwissPalmi | Q64264 |
Expressioni
Tissue specificityi
Most abundantly expressed in midbrain, in dorsal raphe and hippocampus. Detected at lower levels in amygdala and brain cortex.1 Publication
Gene expression databases
Bgeei | ENSMUSG00000021721, Expressed in CA1 field of hippocampus and 101 other tissues |
Genevisiblei | Q64264, MM |
Interactioni
Subunit structurei
Heterodimer; heterodimerizes with GPER1 (By similarity).
Interacts with YIF1B (By similarity).
By similarityGO - Molecular functioni
- G-protein alpha-subunit binding Source: MGI
- receptor-receptor interaction Source: MGI
- signaling receptor binding Source: MGI
Protein-protein interaction databases
STRINGi | 10090.ENSMUSP00000022235 |
Chemistry databases
BindingDBi | Q64264 |
Miscellaneous databases
RNActi | Q64264, protein |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 112 – 121 | Agonist bindingBy similarity | 10 | |
Regioni | 358 – 362 | Agonist bindingBy similarity | 5 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 133 – 135 | DRY motif; important for ligand-induced conformation changesBy similarity | 3 | |
Motifi | 396 – 400 | NPxxY motif; important for ligand-induced conformation changes and signalingBy similarity | 5 |
Sequence similaritiesi
Belongs to the G-protein coupled receptor 1 family. 5-hydroxytryptamine receptor subfamily. HTR1A sub-subfamily.PROSITE-ProRule annotation
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG3656, Eukaryota |
GeneTreei | ENSGT00940000154484 |
HOGENOMi | CLU_009579_11_1_1 |
InParanoidi | Q64264 |
OMAi | DPGYTIY |
OrthoDBi | 703991at2759 |
TreeFami | TF316350 |
Family and domain databases
InterProi | View protein in InterPro IPR000610, 5HT1A_rcpt IPR002231, 5HT_rcpt IPR000276, GPCR_Rhodpsn IPR017452, GPCR_Rhodpsn_7TM |
PANTHERi | PTHR24247:SF20, PTHR24247:SF20, 1 hit |
Pfami | View protein in Pfam PF00001, 7tm_1, 1 hit |
PRINTSi | PR00512, 5HT1ARECEPTR PR01101, 5HTRECEPTOR PR00237, GPCRRHODOPSN |
SMARTi | View protein in SMART SM01381, 7TM_GPCR_Srsx, 1 hit |
PROSITEi | View protein in PROSITE PS00237, G_PROTEIN_RECEP_F1_1, 1 hit PS50262, G_PROTEIN_RECEP_F1_2, 1 hit |
i Sequence
Sequence statusi: Complete.
Q64264-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MDMFSLGQGN NTTTSLEPFG TGGNDTGLSN VTFSYQVITS LLLGTLIFCA
60 70 80 90 100
VLGNACVVAA IALERSLQNV ANYLIGSLAV TDLMVSVLVL PMAALYQVLN
110 120 130 140 150
KWTLGQVTCD LFIALDVLCC TSSILHLCAI ALDRYWAITD PIDYVNKRTP
160 170 180 190 200
RRAAALISLT WLIGFLISIP PMLGWRTPED RSNPNECTIS KDHGYTIYST
210 220 230 240 250
FGAFYIPLLL MLVLYGRIFR AARFRIRKTV KKVEKKGAGT SFGTSSAPPP
260 270 280 290 300
KKSLNGQPGS GDCRRSAENR AVGTPCANGA VRQGEDDATL EVIEVHRVGN
310 320 330 340 350
SKGHLPLPSE SGATSYVPAC LERKNERTAE AKRKMALARE RKTVKTLGII
360 370 380 390 400
MGTFILCWLP FFIVALVLPF CESSCHMPEL LGAIINWLGY SNSLLNPVIY
410 420
AYFNKDFQNA FKKIIKCKFC R
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 177 | T → A in AAA81519 (PubMed:8254366).Curated | 1 | |
Sequence conflicti | 242 | F → L in AAA16850 (PubMed:8288218).Curated | 1 | |
Sequence conflicti | 247 | A → V in AAA16850 (PubMed:8288218).Curated | 1 | |
Sequence conflicti | 263 | C → W in AAA16850 (PubMed:8288218).Curated | 1 | |
Sequence conflicti | 304 | H → D in AAA81519 (PubMed:8254366).Curated | 1 |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural varianti | 17 – 19 | EPF → DHL in strain: C3H/An. | 3 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U39391 mRNA Translation: AAA81519.1 AK043668 mRNA Translation: BAC31611.1 AK049814 mRNA Translation: BAC33931.1 AK049884 mRNA Translation: BAC33970.1 AK140194 mRNA Translation: BAE24273.1 CH466568 Genomic DNA Translation: EDL18479.1 BC138669 mRNA Translation: AAI38670.1 BC138681 mRNA Translation: AAI38682.1 U33820 Genomic DNA Translation: AAC52572.1 L20339 mRNA Translation: AAA16850.1 |
CCDSi | CCDS26756.1 |
PIRi | I49375 |
RefSeqi | NP_032334.2, NM_008308.4 |
Genome annotation databases
Ensembli | ENSMUST00000022235; ENSMUSP00000022235; ENSMUSG00000021721 |
GeneIDi | 15550 |
KEGGi | mmu:15550 |
UCSCi | uc007rtu.2, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U39391 mRNA Translation: AAA81519.1 AK043668 mRNA Translation: BAC31611.1 AK049814 mRNA Translation: BAC33931.1 AK049884 mRNA Translation: BAC33970.1 AK140194 mRNA Translation: BAE24273.1 CH466568 Genomic DNA Translation: EDL18479.1 BC138669 mRNA Translation: AAI38670.1 BC138681 mRNA Translation: AAI38682.1 U33820 Genomic DNA Translation: AAC52572.1 L20339 mRNA Translation: AAA16850.1 |
CCDSi | CCDS26756.1 |
PIRi | I49375 |
RefSeqi | NP_032334.2, NM_008308.4 |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
STRINGi | 10090.ENSMUSP00000022235 |
Chemistry databases
BindingDBi | Q64264 |
ChEMBLi | CHEMBL3737 |
DrugCentrali | Q64264 |
Protein family/group databases
GPCRDBi | Search... |
PTM databases
GlyGeni | Q64264, 4 sites |
iPTMneti | Q64264 |
PhosphoSitePlusi | Q64264 |
SwissPalmi | Q64264 |
Proteomic databases
MaxQBi | Q64264 |
PaxDbi | Q64264 |
PRIDEi | Q64264 |
ProteomicsDBi | 285688 |
Protocols and materials databases
Antibodypediai | 2959, 319 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000022235; ENSMUSP00000022235; ENSMUSG00000021721 |
GeneIDi | 15550 |
KEGGi | mmu:15550 |
UCSCi | uc007rtu.2, mouse |
Organism-specific databases
CTDi | 3350 |
MGIi | MGI:96273, Htr1a |
Phylogenomic databases
eggNOGi | KOG3656, Eukaryota |
GeneTreei | ENSGT00940000154484 |
HOGENOMi | CLU_009579_11_1_1 |
InParanoidi | Q64264 |
OMAi | DPGYTIY |
OrthoDBi | 703991at2759 |
TreeFami | TF316350 |
Enzyme and pathway databases
Reactomei | R-MMU-390666, Serotonin receptors |
Miscellaneous databases
BioGRID-ORCSi | 15550, 0 hits in 52 CRISPR screens |
PROi | PR:Q64264 |
RNActi | Q64264, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000021721, Expressed in CA1 field of hippocampus and 101 other tissues |
Genevisiblei | Q64264, MM |
Family and domain databases
InterProi | View protein in InterPro IPR000610, 5HT1A_rcpt IPR002231, 5HT_rcpt IPR000276, GPCR_Rhodpsn IPR017452, GPCR_Rhodpsn_7TM |
PANTHERi | PTHR24247:SF20, PTHR24247:SF20, 1 hit |
Pfami | View protein in Pfam PF00001, 7tm_1, 1 hit |
PRINTSi | PR00512, 5HT1ARECEPTR PR01101, 5HTRECEPTOR PR00237, GPCRRHODOPSN |
SMARTi | View protein in SMART SM01381, 7TM_GPCR_Srsx, 1 hit |
PROSITEi | View protein in PROSITE PS00237, G_PROTEIN_RECEP_F1_1, 1 hit PS50262, G_PROTEIN_RECEP_F1_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | 5HT1A_MOUSE | |
Accessioni | Q64264Primary (citable) accession number: Q64264 Secondary accession number(s): Q60956, Q61617, Q8BGS4 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 1, 1997 |
Last sequence update: | July 27, 2011 | |
Last modified: | April 7, 2021 | |
This is version 173 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- 7-transmembrane G-linked receptors
List of 7-transmembrane G-linked receptor entries - MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families