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UniProtKB - Q64249 (NR6A1_MOUSE)
Protein
Nuclear receptor subfamily 6 group A member 1
Gene
Nr6a1
Organism
Mus musculus (Mouse)
Status
Functioni
Orphan nuclear receptor. Binds to a response element containing the sequence 5'-TCAAGGTCA-3'. May be involved in the regulation of gene expression in germ cell development during gametogenesis.
1 PublicationRegions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
DNA bindingi | 72 – 147 | Nuclear receptorPROSITE-ProRule annotationAdd BLAST | 76 | |
Zinc fingeri | 75 – 95 | NR C4-typePROSITE-ProRule annotationAdd BLAST | 21 | |
Zinc fingeri | 111 – 135 | NR C4-typePROSITE-ProRule annotationAdd BLAST | 25 |
GO - Molecular functioni
- DNA-binding transcription activator activity, RNA polymerase II-specific Source: NTNU_SB
- nuclear receptor activity Source: GO_Central
- protein homodimerization activity Source: UniProtKB
- RNA polymerase II cis-regulatory region sequence-specific DNA binding Source: MGI
- sequence-specific DNA binding Source: UniProtKB
- sequence-specific double-stranded DNA binding Source: MGI
- zinc ion binding Source: InterPro
GO - Biological processi
- cell differentiation Source: UniProtKB-KW
- gamete generation Source: UniProtKB
- negative regulation of transcription by RNA polymerase II Source: MGI
- positive regulation of transcription by RNA polymerase II Source: NTNU_SB
- regulation of transcription by RNA polymerase II Source: GO_Central
- spermatogenesis Source: UniProtKB-KW
Keywordsi
Molecular function | Developmental protein, DNA-binding, Receptor |
Biological process | Differentiation, Spermatogenesis, Transcription, Transcription regulation |
Ligand | Metal-binding, Zinc |
Enzyme and pathway databases
Reactomei | R-MMU-383280, Nuclear Receptor transcription pathway |
Names & Taxonomyi
Protein namesi | Recommended name: Nuclear receptor subfamily 6 group A member 1Alternative name(s): Germ cell nuclear factor Short name: GCNF Short name: mGCNF Retinoid receptor-related testis-specific receptor Short name: RTR |
Gene namesi | Name:Nr6a1 Synonyms:Gcnf |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1352459, Nr6a1 |
VEuPathDBi | HostDB:ENSMUSG00000063972 |
Subcellular locationi
Nucleus
- Nucleus Curated
Nucleus
- nucleus Source: UniProtKB-SubCell
Other locations
- transcription regulator complex Source: MGI
Keywords - Cellular componenti
NucleusPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 246 – 247 | SY → GG: Abolishes interaction with RXRIP110. 1 Publication | 2 | |
Mutagenesisi | 254 – 255 | LP → AA: Does not abolish interaction with RXRIP110. 1 Publication | 2 | |
Mutagenesisi | 265 – 266 | SY → AA: Does not abolish interaction with RXRIP110. 1 Publication | 2 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000053742 | 1 – 495 | Nuclear receptor subfamily 6 group A member 1Add BLAST | 495 |
Proteomic databases
PaxDbi | Q64249 |
PRIDEi | Q64249 |
ProteomicsDBi | 252849 [Q64249-1] 252850 [Q64249-2] |
PTM databases
PhosphoSitePlusi | Q64249 |
Expressioni
Tissue specificityi
Expressed in the germ cells of both the adult testis and ovary, being most abundant in spermatids.2 Publications
Gene expression databases
Bgeei | ENSMUSG00000063972, Expressed in primitive streak and 241 other tissues |
ExpressionAtlasi | Q64249, baseline and differential |
Genevisiblei | Q64249, MM |
Interactioni
Subunit structurei
Homodimer.
Interacts with UIMC1.
2 PublicationsGO - Molecular functioni
- protein homodimerization activity Source: UniProtKB
Protein-protein interaction databases
BioGRIDi | 199868, 16 interactors |
STRINGi | 10090.ENSMUSP00000108498 |
Miscellaneous databases
RNActi | Q64249, protein |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | Q64249 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 264 – 495 | NR LBDPROSITE-ProRule annotationAdd BLAST | 232 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 34 | DisorderedSequence analysisAdd BLAST | 34 | |
Regioni | 145 – 165 | DisorderedSequence analysisAdd BLAST | 21 | |
Regioni | 177 – 214 | DisorderedSequence analysisAdd BLAST | 38 | |
Regioni | 187 – 268 | Sufficient for interaction with UIMC1Add BLAST | 82 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 188 – 214 | Polar residuesSequence analysisAdd BLAST | 27 |
Sequence similaritiesi
Zinc finger
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 75 – 95 | NR C4-typePROSITE-ProRule annotationAdd BLAST | 21 | |
Zinc fingeri | 111 – 135 | NR C4-typePROSITE-ProRule annotationAdd BLAST | 25 |
Keywords - Domaini
Zinc-fingerPhylogenomic databases
eggNOGi | KOG3575, Eukaryota |
GeneTreei | ENSGT00940000157936 |
HOGENOMi | CLU_007368_6_1_1 |
InParanoidi | Q64249 |
OMAi | SPEAYVC |
OrthoDBi | 619653at2759 |
PhylomeDBi | Q64249 |
TreeFami | TF350737 |
Family and domain databases
Gene3Di | 1.10.565.10, 1 hit 3.30.50.10, 1 hit |
InterProi | View protein in InterPro IPR035500, NHR-like_dom_sf IPR000536, Nucl_hrmn_rcpt_lig-bd IPR001723, Nuclear_hrmn_rcpt IPR001628, Znf_hrmn_rcpt IPR013088, Znf_NHR/GATA |
Pfami | View protein in Pfam PF00104, Hormone_recep, 1 hit PF00105, zf-C4, 1 hit |
PRINTSi | PR00398, STRDHORMONER PR00047, STROIDFINGER |
SMARTi | View protein in SMART SM00430, HOLI, 1 hit SM00399, ZnF_C4, 1 hit |
SUPFAMi | SSF48508, SSF48508, 1 hit |
PROSITEi | View protein in PROSITE PS51843, NR_LBD, 1 hit PS00031, NUCLEAR_REC_DBD_1, 1 hit PS51030, NUCLEAR_REC_DBD_2, 1 hit |
s (3+)i Sequence
Sequence statusi: Complete.
This entry describes 3 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 3 described isoforms and 2 potential isoforms that are computationally mapped.Show allAlign All
Isoform 1 (identifier: Q64249-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MERDERPPSG GGGGGGSAGF LEPPAALPPP PRNGFCQDEL AELDPGTNGE
60 70 80 90 100
TDSLTLGQGH IPVSVPDDRA EQRTCLICGD RATGLHYGII SCEGCKGFFK
110 120 130 140 150
RSICNKRVYR CSRDKNCVMS RKQRNRCQYC RLLKCLQMGM NRKAIREDGM
160 170 180 190 200
PGGRNKSIGP VQISEEEIER IMSGQEFEEE ANHWSNHGDS DHSSPGNRAS
210 220 230 240 250
ESNQPSPGST LSSSRSVELN GFMAFRDQYM GMSVPPHYQY IPHLFSYSGH
260 270 280 290 300
SPLLPPQARS LDPQSYSLIH QLMSAEDLEP LGTPMLIEDG YAVTQAELFA
310 320 330 340 350
LLCRLADELL FRQIAWIKKL PFFCELSIKD YTCLLSSTWQ ELILLSSLTV
360 370 380 390 400
YSKQIFGELA DVTAKYSPSD EELHRFSDEG MEVIERLIYL YHKFHQLKVS
410 420 430 440 450
NEEYACMKAI NFLNQDIRGL TSASQLEQLN KRYWYICQDF TEYKYTHQPN
460 470 480 490
RFPDLMMCLP EIRYIAGKMV NVPLEQLPLL FKVVLHSCKT STVKE
Computationally mapped potential isoform sequencesi
There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketA0A0A0MQG4 | A0A0A0MQG4_MOUSE | Nuclear receptor subfamily 6 group ... | Nr6a1 | 220 | Annotation score: | ||
Z4YMW4 | Z4YMW4_MOUSE | Nuclear receptor subfamily 6 group ... | Nr6a1 | 235 | Annotation score: |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_025928 | 1 – 57 | Missing in isoform 3. CuratedAdd BLAST | 57 | |
Alternative sequenceiVSP_025929 | 48 – 62 | Missing in isoform 2. CuratedAdd BLAST | 15 | |
Alternative sequenceiVSP_025930 | 58 – 62 | QGHIP → METWE in isoform 3. Curated | 5 | |
Alternative sequenceiVSP_025931 | 63 | V → I in isoform 2. Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U14666 mRNA Translation: AAA92682.1 U09563 mRNA Translation: AAA64746.1 AF254821 , AF254575, AF254815, AF254816, AF254817, AF254818, AF254819, AF254820 Genomic DNA Translation: AAG24485.1 AF390896 mRNA Translation: AAM55193.1 AF390900 , AF390897, AF390898, AF390899 Genomic DNA Translation: AAM95878.1 AL844842 Genomic DNA No translation available. AL935155 Genomic DNA No translation available. |
CCDSi | CCDS16012.1 [Q64249-1] CCDS50581.1 [Q64249-3] |
PIRi | A57053 |
RefSeqi | NP_001153020.1, NM_001159548.1 [Q64249-3] NP_034394.1, NM_010264.4 [Q64249-1] |
Genome annotation databases
Ensembli | ENSMUST00000076275; ENSMUSP00000075624; ENSMUSG00000063972 [Q64249-1] ENSMUST00000112877; ENSMUSP00000108498; ENSMUSG00000063972 [Q64249-1] ENSMUST00000168098; ENSMUSP00000126009; ENSMUSG00000063972 [Q64249-3] |
GeneIDi | 14536 |
KEGGi | mmu:14536 |
UCSCi | uc012bus.1, mouse [Q64249-3] uc012buu.1, mouse [Q64249-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U14666 mRNA Translation: AAA92682.1 U09563 mRNA Translation: AAA64746.1 AF254821 , AF254575, AF254815, AF254816, AF254817, AF254818, AF254819, AF254820 Genomic DNA Translation: AAG24485.1 AF390896 mRNA Translation: AAM55193.1 AF390900 , AF390897, AF390898, AF390899 Genomic DNA Translation: AAM95878.1 AL844842 Genomic DNA No translation available. AL935155 Genomic DNA No translation available. |
CCDSi | CCDS16012.1 [Q64249-1] CCDS50581.1 [Q64249-3] |
PIRi | A57053 |
RefSeqi | NP_001153020.1, NM_001159548.1 [Q64249-3] NP_034394.1, NM_010264.4 [Q64249-1] |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
5KRB | X-ray | 2.10 | B/G | 72-155 | [»] | |
SMRi | Q64249 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 199868, 16 interactors |
STRINGi | 10090.ENSMUSP00000108498 |
PTM databases
PhosphoSitePlusi | Q64249 |
Proteomic databases
PaxDbi | Q64249 |
PRIDEi | Q64249 |
ProteomicsDBi | 252849 [Q64249-1] 252850 [Q64249-2] |
Protocols and materials databases
Antibodypediai | 16337, 334 antibodies from 35 providers |
DNASUi | 14536 |
Genome annotation databases
Ensembli | ENSMUST00000076275; ENSMUSP00000075624; ENSMUSG00000063972 [Q64249-1] ENSMUST00000112877; ENSMUSP00000108498; ENSMUSG00000063972 [Q64249-1] ENSMUST00000168098; ENSMUSP00000126009; ENSMUSG00000063972 [Q64249-3] |
GeneIDi | 14536 |
KEGGi | mmu:14536 |
UCSCi | uc012bus.1, mouse [Q64249-3] uc012buu.1, mouse [Q64249-1] |
Organism-specific databases
CTDi | 2649 |
MGIi | MGI:1352459, Nr6a1 |
VEuPathDBi | HostDB:ENSMUSG00000063972 |
Phylogenomic databases
eggNOGi | KOG3575, Eukaryota |
GeneTreei | ENSGT00940000157936 |
HOGENOMi | CLU_007368_6_1_1 |
InParanoidi | Q64249 |
OMAi | SPEAYVC |
OrthoDBi | 619653at2759 |
PhylomeDBi | Q64249 |
TreeFami | TF350737 |
Enzyme and pathway databases
Reactomei | R-MMU-383280, Nuclear Receptor transcription pathway |
Miscellaneous databases
BioGRID-ORCSi | 14536, 2 hits in 64 CRISPR screens |
ChiTaRSi | Nr6a1, mouse |
PROi | PR:Q64249 |
RNActi | Q64249, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000063972, Expressed in primitive streak and 241 other tissues |
ExpressionAtlasi | Q64249, baseline and differential |
Genevisiblei | Q64249, MM |
Family and domain databases
Gene3Di | 1.10.565.10, 1 hit 3.30.50.10, 1 hit |
InterProi | View protein in InterPro IPR035500, NHR-like_dom_sf IPR000536, Nucl_hrmn_rcpt_lig-bd IPR001723, Nuclear_hrmn_rcpt IPR001628, Znf_hrmn_rcpt IPR013088, Znf_NHR/GATA |
Pfami | View protein in Pfam PF00104, Hormone_recep, 1 hit PF00105, zf-C4, 1 hit |
PRINTSi | PR00398, STRDHORMONER PR00047, STROIDFINGER |
SMARTi | View protein in SMART SM00430, HOLI, 1 hit SM00399, ZnF_C4, 1 hit |
SUPFAMi | SSF48508, SSF48508, 1 hit |
PROSITEi | View protein in PROSITE PS51843, NR_LBD, 1 hit PS00031, NUCLEAR_REC_DBD_1, 1 hit PS51030, NUCLEAR_REC_DBD_2, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | NR6A1_MOUSE | |
Accessioni | Q64249Primary (citable) accession number: Q64249 Secondary accession number(s): A2AQL8, A2AQM1, Q687Z3 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | December 1, 2000 |
Last sequence update: | November 1, 1996 | |
Last modified: | February 23, 2022 | |
This is version 179 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families