UniProtKB - Q64221 (HEN2_MOUSE)
Helix-loop-helix protein 2
Nhlh2
Functioni
Transcription factor which binds the E box motif 5'-CA[TC][AG]TG-3' (PubMed:15470499, PubMed:18356286).
Involved in regulating energy expenditure, body mass, voluntary physical activity, mating behavior and reproductive longevity, acting through the hypothalamic-pituitary-gonadal axis (PubMed:15470499, PubMed:12419415, PubMed:9090387, PubMed:17717072, PubMed:23785158, PubMed:15465527).
Acts as a transcriptional activator of target genes, including Ndn and Pcsk1 (PubMed:15470499, PubMed:18356286).
May act centrally to regulate function of both white and brown adipose tissue (PubMed:20808804, PubMed:19436734).
Together with NHLH1, required to maintain migration and survival of cells in the anterior extramural migration stream (aes), which forms the precerebellar nuclei (PubMed:17573818).
Also, in concert with Nhlh1, may determine fate of gonadotropin releasing hormone-1 (GnRH-1) neurons (PubMed:15470499).
10 PublicationsCaution
GO - Molecular functioni
- DNA-binding transcription activator activity, RNA polymerase II-specific Source: NTNU_SB
- DNA-binding transcription factor activity, RNA polymerase II-specific Source: GO_Central
- protein dimerization activity Source: InterPro
- RNA polymerase II cis-regulatory region sequence-specific DNA binding Source: NTNU_SB
- RNA polymerase II-specific DNA-binding transcription factor binding Source: UniProtKB
- RNA polymerase II transcription regulatory region sequence-specific DNA binding Source: UniProtKB
- sequence-specific double-stranded DNA binding Source: MGI
GO - Biological processi
- apoptotic process Source: UniProtKB
- cell migration in hindbrain Source: UniProtKB
- hypothalamus gonadotrophin-releasing hormone neuron development Source: UniProtKB
- male gonad development Source: UniProtKB
- male mating behavior Source: UniProtKB
- mating behavior Source: MGI
- ovulation cycle Source: MGI
- peripheral nervous system development Source: UniProtKB
- positive regulation of transcription by RNA polymerase II Source: UniProtKB
- regulation of transcription by RNA polymerase II Source: GO_Central
Keywordsi
Molecular function | Activator, Developmental protein, DNA-binding |
Biological process | Differentiation, Transcription, Transcription regulation |
Names & Taxonomyi
Protein namesi | Recommended name: Helix-loop-helix protein 2Short name: HEN-2 Alternative name(s): Nescient helix loop helix 2 Short name: NSCL-2 |
Gene namesi | Name:Nhlh2 Synonyms:Hen2 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:97324, Nhlh2 |
VEuPathDBi | HostDB:ENSMUSG00000048540 |
Subcellular locationi
Nucleus
- Nucleus PROSITE-ProRule annotation
Keywords - Cellular componenti
NucleusPathology & Biotechi
Disruption phenotypei
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000127200 | 1 – 135 | Helix-loop-helix protein 2Add BLAST | 135 |
Proteomic databases
PaxDbi | Q64221 |
PRIDEi | Q64221 |
ProteomicsDBi | 269590 |
PTM databases
PhosphoSitePlusi | Q64221 |
Expressioni
Tissue specificityi
Developmental stagei
Gene expression databases
Bgeei | ENSMUSG00000048540, Expressed in habenula and 134 other tissues |
Genevisiblei | Q64221, MM |
Interactioni
Subunit structurei
Homodimer.
Interacts and may form heterodimers with STAT3.
1 PublicationBinary interactionsi
Q64221
With | #Exp. | IntAct |
---|---|---|
Sirt1 [Q923E4] | 2 | EBI-5378529,EBI-1802585 |
GO - Molecular functioni
- protein dimerization activity Source: InterPro
- RNA polymerase II-specific DNA-binding transcription factor binding Source: UniProtKB
Protein-protein interaction databases
IntActi | Q64221, 1 interactor |
STRINGi | 10090.ENSMUSP00000064355 |
Miscellaneous databases
RNActi | Q64221, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 77 – 129 | bHLHPROSITE-ProRule annotationAdd BLAST | 53 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 81 | DisorderedSequence analysisAdd BLAST | 81 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 9 – 23 | Polar residuesSequence analysisAdd BLAST | 15 |
Phylogenomic databases
eggNOGi | KOG4029, Eukaryota |
GeneTreei | ENSGT00940000162602 |
InParanoidi | Q64221 |
OMAi | RSCCASD |
OrthoDBi | 1491224at2759 |
PhylomeDBi | Q64221 |
Family and domain databases
Gene3Di | 4.10.280.10, 1 hit |
InterProi | View protein in InterPro IPR011598, bHLH_dom IPR036638, HLH_DNA-bd_sf IPR040238, TAL-like |
PANTHERi | PTHR13864, PTHR13864, 1 hit |
Pfami | View protein in Pfam PF00010, HLH, 1 hit |
SMARTi | View protein in SMART SM00353, HLH, 1 hit |
SUPFAMi | SSF47459, SSF47459, 1 hit |
PROSITEi | View protein in PROSITE PS50888, BHLH, 1 hit |
i Sequence
Sequence statusi: Complete.
10 20 30 40 50
MMLSPDQAAD SDHPSSTHSD PESLGGADTK VLGSVSDLEP VEEADGDGKG
60 70 80 90 100
GSRAALYPHP QQLSREEKRR RRRATAKYRS AHATRERIRV EAFNLAFAEL
110 120 130
RKLLPTLPPD KKLSKIEILR LAICYISYLN HVLDV
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 55 | A → V in AAB22580 (PubMed:1633105).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | S40532 mRNA Translation: AAB22580.1 AK031747 mRNA Translation: BAC27535.1 AK053837 mRNA Translation: BAC35549.1 AK079121 mRNA Translation: BAC37549.1 AK082728 mRNA Translation: BAC38590.1 AK084160 mRNA Translation: BAC39129.1 AK140559 mRNA Translation: BAE24423.1 AC166072 Genomic DNA No translation available. CH466608 Genomic DNA Translation: EDL07620.1 CH466608 Genomic DNA Translation: EDL07621.1 CH466608 Genomic DNA Translation: EDL07622.1 BC058413 mRNA Translation: AAH58413.1 |
CCDSi | CCDS17685.1 |
PIRi | A49005 |
RefSeqi | NP_848892.1, NM_178777.3 XP_006501175.1, XM_006501112.2 |
Genome annotation databases
Ensembli | ENSMUST00000066187; ENSMUSP00000064355; ENSMUSG00000048540 ENSMUST00000196324; ENSMUSP00000142746; ENSMUSG00000048540 ENSMUST00000198675; ENSMUSP00000143362; ENSMUSG00000048540 |
GeneIDi | 18072 |
KEGGi | mmu:18072 |
UCSCi | uc008qro.2, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | S40532 mRNA Translation: AAB22580.1 AK031747 mRNA Translation: BAC27535.1 AK053837 mRNA Translation: BAC35549.1 AK079121 mRNA Translation: BAC37549.1 AK082728 mRNA Translation: BAC38590.1 AK084160 mRNA Translation: BAC39129.1 AK140559 mRNA Translation: BAE24423.1 AC166072 Genomic DNA No translation available. CH466608 Genomic DNA Translation: EDL07620.1 CH466608 Genomic DNA Translation: EDL07621.1 CH466608 Genomic DNA Translation: EDL07622.1 BC058413 mRNA Translation: AAH58413.1 |
CCDSi | CCDS17685.1 |
PIRi | A49005 |
RefSeqi | NP_848892.1, NM_178777.3 XP_006501175.1, XM_006501112.2 |
3D structure databases
AlphaFoldDBi | Q64221 |
SMRi | Q64221 |
ModBasei | Search... |
Protein-protein interaction databases
IntActi | Q64221, 1 interactor |
STRINGi | 10090.ENSMUSP00000064355 |
PTM databases
PhosphoSitePlusi | Q64221 |
Proteomic databases
PaxDbi | Q64221 |
PRIDEi | Q64221 |
ProteomicsDBi | 269590 |
Protocols and materials databases
Antibodypediai | 33870, 192 antibodies from 24 providers |
DNASUi | 18072 |
Genome annotation databases
Ensembli | ENSMUST00000066187; ENSMUSP00000064355; ENSMUSG00000048540 ENSMUST00000196324; ENSMUSP00000142746; ENSMUSG00000048540 ENSMUST00000198675; ENSMUSP00000143362; ENSMUSG00000048540 |
GeneIDi | 18072 |
KEGGi | mmu:18072 |
UCSCi | uc008qro.2, mouse |
Organism-specific databases
CTDi | 4808 |
MGIi | MGI:97324, Nhlh2 |
VEuPathDBi | HostDB:ENSMUSG00000048540 |
Phylogenomic databases
eggNOGi | KOG4029, Eukaryota |
GeneTreei | ENSGT00940000162602 |
InParanoidi | Q64221 |
OMAi | RSCCASD |
OrthoDBi | 1491224at2759 |
PhylomeDBi | Q64221 |
Miscellaneous databases
BioGRID-ORCSi | 18072, 2 hits in 72 CRISPR screens |
ChiTaRSi | Nhlh2, mouse |
PROi | PR:Q64221 |
RNActi | Q64221, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000048540, Expressed in habenula and 134 other tissues |
Genevisiblei | Q64221, MM |
Family and domain databases
Gene3Di | 4.10.280.10, 1 hit |
InterProi | View protein in InterPro IPR011598, bHLH_dom IPR036638, HLH_DNA-bd_sf IPR040238, TAL-like |
PANTHERi | PTHR13864, PTHR13864, 1 hit |
Pfami | View protein in Pfam PF00010, HLH, 1 hit |
SMARTi | View protein in SMART SM00353, HLH, 1 hit |
SUPFAMi | SSF47459, SSF47459, 1 hit |
PROSITEi | View protein in PROSITE PS50888, BHLH, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | HEN2_MOUSE | |
Accessioni | Q64221Primary (citable) accession number: Q64221 Secondary accession number(s): Q8BFV7 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 1, 1997 |
Last sequence update: | October 3, 2012 | |
Last modified: | May 25, 2022 | |
This is version 146 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot