UniProtKB - Q64213 (SF01_MOUSE)
Splicing factor 1
Sf1
Functioni
Necessary for the ATP-dependent first step of spliceosome assembly. Binds to the intron branch point sequence (BPS) 5'-UACUAAC-3' of the pre-mRNA. May act as transcription repressor (By similarity).
By similarityRegions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 277 – 296 | CCHC-typePROSITE-ProRule annotationAdd BLAST | 20 |
GO - Molecular functioni
- identical protein binding Source: MGI
- mRNA binding Source: GO_Central
- pre-mRNA branch point binding Source: InterPro
- RNA binding Source: MGI
- zinc ion binding Source: InterPro
GO - Biological processi
- Leydig cell differentiation Source: MGI
- male sex determination Source: MGI
- mRNA splicing, via spliceosome Source: InterPro
- negative regulation of smooth muscle cell proliferation Source: MGI
- nuclear body organization Source: MGI
- regulation of mRNA splicing, via spliceosome Source: GO_Central
- regulation of steroid biosynthetic process Source: MGI
Keywordsi
Molecular function | Repressor, RNA-binding |
Biological process | mRNA processing, mRNA splicing, Transcription, Transcription regulation |
Ligand | Metal-binding, Zinc |
Enzyme and pathway databases
Reactomei | R-MMU-72163, mRNA Splicing - Major Pathway |
Names & Taxonomyi
Protein namesi | Recommended name: Splicing factor 1Alternative name(s): CW17 Mammalian branch point-binding protein Short name: BBP Short name: mBBP Transcription factor ZFM1 Short name: mZFM Zinc finger gene in MEN1 locus Zinc finger protein 162 |
Gene namesi | Name:Sf1 Synonyms:Zfm1, Zfp162 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1095403, Sf1 |
VEuPathDBi | HostDB:ENSMUSG00000024949 |
Subcellular locationi
Nucleus
Nucleus
- nuclear body Source: MGI
- nucleoplasm Source: MGI
- nucleus Source: MGI
- spliceosomal complex Source: MGI
- U2AF complex Source: Ensembl
Other locations
- cytoplasm Source: MGI
Keywords - Cellular componenti
Nucleus, SpliceosomePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | RemovedBy similarity | |||
ChainiPRO_0000050130 | 2 – 653 | Splicing factor 1Add BLAST | 652 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 2 | N-acetylalanineBy similarity | 1 | |
Modified residuei | 14 | PhosphoserineBy similarity | 1 | |
Modified residuei | 20 | Phosphoserine; by PKGBy similarity | 1 | |
Modified residuei | 80 | PhosphoserineCombined sources | 1 | |
Modified residuei | 82 | PhosphoserineCombined sources | 1 | |
Modified residuei | 87 | PhosphotyrosineCombined sources | 1 | |
Modified residuei | 89 | PhosphoserineBy similarity | 1 |
Post-translational modificationi
Keywords - PTMi
Acetylation, PhosphoproteinProteomic databases
EPDi | Q64213 |
jPOSTi | Q64213 |
MaxQBi | Q64213 |
PaxDbi | Q64213 |
PeptideAtlasi | Q64213 |
PRIDEi | Q64213 |
ProteomicsDBi | 261501 [Q64213-1] 261502 [Q64213-2] 261503 [Q64213-3] |
PTM databases
iPTMneti | Q64213 |
PhosphoSitePlusi | Q64213 |
SwissPalmi | Q64213 |
Expressioni
Tissue specificityi
Gene expression databases
Bgeei | ENSMUSG00000024949, Expressed in ear and 310 other tissues |
ExpressionAtlasi | Q64213, baseline and differential |
Genevisiblei | Q64213, MM |
Interactioni
Subunit structurei
Binds U2AF2.
Interacts with U1 snRNA.
Interacts with RBM17. Binds EWSR1, FUS and TAF15 (By similarity).
By similarityGO - Molecular functioni
- identical protein binding Source: MGI
Protein-protein interaction databases
ComplexPortali | CPX-5861, SF1-U2AF65 splicing factor complex |
DIPi | DIP-60453N |
ELMi | Q64213 |
IntActi | Q64213, 2 interactors |
STRINGi | 10090.ENSMUSP00000121309 |
Miscellaneous databases
RNActi | Q64213, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 141 – 222 | KHPROSITE-ProRule annotationAdd BLAST | 82 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 44 | DisorderedSequence analysisAdd BLAST | 44 | |
Regioni | 65 – 94 | DisorderedSequence analysisAdd BLAST | 30 | |
Regioni | 325 – 584 | DisorderedSequence analysisAdd BLAST | 260 | |
Regioni | 611 – 653 | DisorderedSequence analysisAdd BLAST | 43 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 15 – 19 | Nuclear localization signalSequence analysis | 5 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 346 – 361 | Pro residuesSequence analysisAdd BLAST | 16 | |
Compositional biasi | 415 – 445 | Pro residuesSequence analysisAdd BLAST | 31 | |
Compositional biasi | 472 – 518 | Pro residuesSequence analysisAdd BLAST | 47 | |
Compositional biasi | 519 – 563 | Polar residuesSequence analysisAdd BLAST | 45 | |
Compositional biasi | 564 – 584 | Pro residuesSequence analysisAdd BLAST | 21 |
Sequence similaritiesi
Zinc finger
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 277 – 296 | CCHC-typePROSITE-ProRule annotationAdd BLAST | 20 |
Keywords - Domaini
Zinc-fingerPhylogenomic databases
eggNOGi | KOG0119, Eukaryota |
GeneTreei | ENSGT00940000157258 |
InParanoidi | Q64213 |
OMAi | GAAPWHQ |
TreeFami | TF319159 |
Family and domain databases
Gene3Di | 3.30.1370.10, 1 hit |
InterProi | View protein in InterPro IPR045071, BBP-like IPR031150, BBP/SF1 IPR004087, KH_dom IPR004088, KH_dom_type_1 IPR036612, KH_dom_type_1_sf IPR032570, SF1-HH IPR001878, Znf_CCHC |
PANTHERi | PTHR11208, PTHR11208, 1 hit PTHR11208:SF45, PTHR11208:SF45, 1 hit |
Pfami | View protein in Pfam PF00013, KH_1, 1 hit PF16275, SF1-HH, 1 hit PF00098, zf-CCHC, 1 hit |
SMARTi | View protein in SMART SM00322, KH, 1 hit SM00343, ZnF_C2HC, 1 hit |
SUPFAMi | SSF54791, SSF54791, 1 hit |
PROSITEi | View protein in PROSITE PS50084, KH_TYPE_1, 1 hit PS50158, ZF_CCHC, 1 hit |
s (3+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry describes 3 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 3 described isoforms and 9 potential isoforms that are computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MATGANATPL DFPSKKRKRS RWNQDTMEQK TVIPGMPTVI PPGLTREQER
60 70 80 90 100
AYIVQLQIED LTRKLRTGDL GIPPNPEDRS PSPEPIYNSE GKRLNTREFR
110 120 130 140 150
TRKKLEEERH TLITEMVALN PDFKPPADYK PPATRVSDKV MIPQDEYPEI
160 170 180 190 200
NFVGLLIGPR GNTLKNIEKE CNAKIMIRGK GSVKEGKVGR KDGQMLPGED
210 220 230 240 250
EPLHALVTAN TMENVKKAVE QIRNILKQGI ETPEDQNDLR KMQLRELARL
260 270 280 290 300
NGTLREDDNR ILRPWQSSET RSITNTTVCT KCGGAGHIAS DCKFQRPGDP
310 320 330 340 350
QSAQDKARMD KEYLSLMAEL GEAPVPASVG STSGPATTPL ASAPRPAAPA
360 370 380 390 400
SNPPPPSLMS TTQSRPPWMN SGPSENRPYH GMHGGGPGGP GGGPHSFPHP
410 420 430 440 450
LPSLTGGHGG HPMQHNPNGP PPPWMQPPPP PMNQGPHPPG HHGPPPMDQY
460 470 480 490 500
LGSTPVGSGV YRLHQGKGMM PPPPMGMMPP PPPPPSGQPP PPPSGPLPPW
510 520 530 540 550
QQQQQQPPPP PPPSSSMASS TPLPWQQNTT TTTTSAGTGS IPPWQQQQAA
560 570 580 590 600
AAASPGTPQM QGNPTMVPLP PGVQPPLPPG APPPPPCSIE CLLCLLSSPN
610 620 630 640 650
SLCLSPNRAA RIPPRGSDGP SHESEDFPRP LVTLPGRQPQ QRPWWTGWFG
KAA
The sequence of this isoform differs from the canonical sequence as follows:
529-653: TTTTTTSAGT...WWTGWFGKAA → SLPAAAMARAMRVRTFRAHW
Computationally mapped potential isoform sequencesi
There are 9 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketD3YZC9 | D3YZC9_MOUSE | Splicing factor 1 | Sf1 | 571 | Annotation score: | ||
E9Q4Q2 | E9Q4Q2_MOUSE | Splicing factor 1 | Sf1 | 548 | Annotation score: | ||
D3YZD0 | D3YZD0_MOUSE | Splicing factor 1 | Sf1 | 638 | Annotation score: | ||
D3YVH4 | D3YVH4_MOUSE | Splicing factor 1 | Sf1 | 267 | Annotation score: | ||
F6SRV1 | F6SRV1_MOUSE | Splicing factor 1 | Sf1 | 171 | Annotation score: | ||
F6YR19 | F6YR19_MOUSE | Splicing factor 1 | Sf1 | 225 | Annotation score: | ||
F6Z9W2 | F6Z9W2_MOUSE | Splicing factor 1 | Sf1 | 200 | Annotation score: | ||
F8WHF9 | F8WHF9_MOUSE | Splicing factor 1 | Sf1 | 110 | Annotation score: | ||
D6RDB7 | D6RDB7_MOUSE | Splicing factor 1 | Sf1 | 84 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 184 | K → E in CAA59797 (PubMed:9212169).Curated | 1 | |
Sequence conflicti | 184 | K → E in CAA73359 (PubMed:10360842).Curated | 1 | |
Sequence conflicti | 509 | P → S in AAH55370 (PubMed:15489334).Curated | 1 | |
Sequence conflicti | 524 | P → L in AAH09091 (PubMed:15489334).Curated | 1 | |
Sequence conflicti | 586 | P → T in CAA73359 (PubMed:10360842).Curated | 1 | |
Sequence conflicti | 586 | P → T in CAA70113 (PubMed:10360842).Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_050424 | 529 – 653 | TTTTT…FGKAA → SLPAAAMARAMRVRTFRAHW in isoform CW17E. 2 PublicationsAdd BLAST | 125 | |
Alternative sequenceiVSP_008840 | 586 – 653 | PCSIE…FGKAA → PPPPPGSAGMMYAPPPPPPP PMDPSNFVTMMGMGVAGMPP FGMPPAPPPPPPQN in isoform 3. 1 PublicationAdd BLAST | 68 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X80159 mRNA Translation: CAA56440.1 X85802 mRNA Translation: CAA59797.1 Y08907 Genomic DNA Translation: CAA70113.1 Y12838 Genomic DNA Translation: CAA73359.1 AC127556 Genomic DNA No translation available. AC167245 Genomic DNA No translation available. BC009091 mRNA Translation: AAH09091.1 BC055370 mRNA Translation: AAH55370.1 |
CCDSi | CCDS50368.1 [Q64213-3] CCDS50369.1 [Q64213-2] |
Genome annotation databases
Ensembli | ENSMUST00000131252; ENSMUSP00000121309; ENSMUSG00000024949 [Q64213-3] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X80159 mRNA Translation: CAA56440.1 X85802 mRNA Translation: CAA59797.1 Y08907 Genomic DNA Translation: CAA70113.1 Y12838 Genomic DNA Translation: CAA73359.1 AC127556 Genomic DNA No translation available. AC167245 Genomic DNA No translation available. BC009091 mRNA Translation: AAH09091.1 BC055370 mRNA Translation: AAH55370.1 |
CCDSi | CCDS50368.1 [Q64213-3] CCDS50369.1 [Q64213-2] |
3D structure databases
BMRBi | Q64213 |
SMRi | Q64213 |
ModBasei | Search... |
Protein-protein interaction databases
ComplexPortali | CPX-5861, SF1-U2AF65 splicing factor complex |
DIPi | DIP-60453N |
ELMi | Q64213 |
IntActi | Q64213, 2 interactors |
STRINGi | 10090.ENSMUSP00000121309 |
PTM databases
iPTMneti | Q64213 |
PhosphoSitePlusi | Q64213 |
SwissPalmi | Q64213 |
Proteomic databases
EPDi | Q64213 |
jPOSTi | Q64213 |
MaxQBi | Q64213 |
PaxDbi | Q64213 |
PeptideAtlasi | Q64213 |
PRIDEi | Q64213 |
ProteomicsDBi | 261501 [Q64213-1] 261502 [Q64213-2] 261503 [Q64213-3] |
Protocols and materials databases
Antibodypediai | 15586, 417 antibodies from 31 providers |
Genome annotation databases
Ensembli | ENSMUST00000131252; ENSMUSP00000121309; ENSMUSG00000024949 [Q64213-3] |
Organism-specific databases
MGIi | MGI:1095403, Sf1 |
VEuPathDBi | HostDB:ENSMUSG00000024949 |
Phylogenomic databases
eggNOGi | KOG0119, Eukaryota |
GeneTreei | ENSGT00940000157258 |
InParanoidi | Q64213 |
OMAi | GAAPWHQ |
TreeFami | TF319159 |
Enzyme and pathway databases
Reactomei | R-MMU-72163, mRNA Splicing - Major Pathway |
Miscellaneous databases
ChiTaRSi | Sf1, mouse |
PROi | PR:Q64213 |
RNActi | Q64213, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000024949, Expressed in ear and 310 other tissues |
ExpressionAtlasi | Q64213, baseline and differential |
Genevisiblei | Q64213, MM |
Family and domain databases
Gene3Di | 3.30.1370.10, 1 hit |
InterProi | View protein in InterPro IPR045071, BBP-like IPR031150, BBP/SF1 IPR004087, KH_dom IPR004088, KH_dom_type_1 IPR036612, KH_dom_type_1_sf IPR032570, SF1-HH IPR001878, Znf_CCHC |
PANTHERi | PTHR11208, PTHR11208, 1 hit PTHR11208:SF45, PTHR11208:SF45, 1 hit |
Pfami | View protein in Pfam PF00013, KH_1, 1 hit PF16275, SF1-HH, 1 hit PF00098, zf-CCHC, 1 hit |
SMARTi | View protein in SMART SM00322, KH, 1 hit SM00343, ZnF_C2HC, 1 hit |
SUPFAMi | SSF54791, SSF54791, 1 hit |
PROSITEi | View protein in PROSITE PS50084, KH_TYPE_1, 1 hit PS50158, ZF_CCHC, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | SF01_MOUSE | |
Accessioni | Q64213Primary (citable) accession number: Q64213 Secondary accession number(s): E9QK02 Q921Z4 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 7, 2003 |
Last sequence update: | July 27, 2011 | |
Last modified: | February 23, 2022 | |
This is version 172 of the entry and version 6 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families