UniProtKB - Q641Y2 (NDUS2_RAT)
Protein
NADH dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrial
Gene
Ndufs2
Organism
Rattus norvegicus (Rat)
Status
Functioni
Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.By similarity
Catalytic activityi
- EC:7.1.1.2By similarity
Cofactori
[4Fe-4S] clusterCuratedNote: Binds 1 [4Fe-4S] cluster.Curated
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 326 | Iron-sulfur (4Fe-4S)Curated | 1 | |
Metal bindingi | 332 | Iron-sulfur (4Fe-4S)Curated | 1 | |
Metal bindingi | 347 | Iron-sulfur (4Fe-4S)Curated | 1 |
GO - Molecular functioni
- 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
- metal ion binding Source: UniProtKB-KW
- NAD binding Source: InterPro
- NADH dehydrogenase (ubiquinone) activity Source: GO_Central
- quinone binding Source: InterPro
- ubiquitin protein ligase binding Source: RGD
GO - Biological processi
- mitochondrial ATP synthesis coupled electron transport Source: RGD
- mitochondrial electron transport, NADH to ubiquinone Source: GO_Central
- response to oxidative stress Source: RGD
Keywordsi
Molecular function | Oxidoreductase, Translocase |
Biological process | Electron transport, Respiratory chain, Transport |
Ligand | 4Fe-4S, Iron, Iron-sulfur, Metal-binding, NAD, Ubiquinone |
Enzyme and pathway databases
Reactomei | R-RNO-611105, Respiratory electron transport R-RNO-6799198, Complex I biogenesis |
Names & Taxonomyi
Protein namesi | Recommended name: NADH dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrial (EC:7.1.1.2)Alternative name(s): Complex I-49kD Short name: CI-49kD NADH-ubiquinone oxidoreductase 49 kDa subunit |
Gene namesi | Name:Ndufs2Imported |
Organismi | Rattus norvegicus (Rat) |
Taxonomic identifieri | 10116 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Rattus |
Proteomesi |
|
Organism-specific databases
RGDi | 1307109, Ndufs2 |
Subcellular locationi
Mitochondrion
- Mitochondrion inner membrane 1 Publication; Peripheral membrane protein 1 Publication; Matrix side 1 Publication
Mitochondrion
- mitochondrial respiratory chain complex I Source: RGD
Keywords - Cellular componenti
Membrane, Mitochondrion, Mitochondrion inner membranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transit peptidei | 1 – 33 | MitochondrionBy similarityAdd BLAST | 33 | |
ChainiPRO_0000342702 | 34 – 463 | NADH dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrialAdd BLAST | 430 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 62 | N6-acetyllysineBy similarity | 1 | |
Modified residuei | 118 | Symmetric dimethylarginineBy similarity | 1 |
Post-translational modificationi
Dimethylation at Arg-118 by NDUFAF7 takes place after NDUFS2 assembles into the complex I, leading to stabilize the early intermediate complex.By similarity
Keywords - PTMi
Acetylation, MethylationProteomic databases
jPOSTi | Q641Y2 |
PaxDbi | Q641Y2 |
PRIDEi | Q641Y2 |
2D gel databases
World-2DPAGEi | 0004:Q641Y2 |
PTM databases
iPTMneti | Q641Y2 |
PhosphoSitePlusi | Q641Y2 |
Expressioni
Gene expression databases
Bgeei | ENSRNOG00000038372, Expressed in heart and 21 other tissues |
Genevisiblei | Q641Y2, RN |
Interactioni
Subunit structurei
Complex I is composed of 45 different subunits.
Component of the iron-sulfur (IP) fragment of the enzyme.
Interacts with NDUFAF3.
Interacts with NDUFAF7.
By similarityGO - Molecular functioni
- ubiquitin protein ligase binding Source: RGD
Protein-protein interaction databases
BioGRIDi | 252831, 2 interactors |
STRINGi | 10116.ENSRNOP00000055224 |
Family & Domainsi
Sequence similaritiesi
Belongs to the complex I 49 kDa subunit family.Sequence analysis
Keywords - Domaini
Transit peptidePhylogenomic databases
eggNOGi | KOG2870, Eukaryota |
GeneTreei | ENSGT00390000009529 |
HOGENOMi | CLU_015134_1_1_1 |
InParanoidi | Q641Y2 |
OMAi | IMGTSME |
OrthoDBi | 444312at2759 |
PhylomeDBi | Q641Y2 |
Family and domain databases
Gene3Di | 1.10.645.20, 1 hit |
HAMAPi | MF_01358, NDH1_NuoD, 1 hit |
InterProi | View protein in InterPro IPR001135, NADH_Q_OxRdtase_suD IPR038290, NADH_Q_OxRdtase_suD_sf IPR014029, NADH_UbQ_OxRdtase_49kDa_CS IPR022885, NDH1_su_D/H IPR029014, NiFe-Hase_large |
Pfami | View protein in Pfam PF00346, Complex1_49kDa, 1 hit |
SUPFAMi | SSF56762, SSF56762, 1 hit |
TIGRFAMsi | TIGR01962, NuoD, 1 hit |
PROSITEi | View protein in PROSITE PS00535, COMPLEX1_49K, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
Q641Y2-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MAALRAVCSL RGVGAQVLRA GSGIRLPSQP SRGARRWQPD IEWAEQFSGA
60 70 80 90 100
VMYPSKETAH WKPPPWNDVD VLKEKVVTNV TLNFGPQHPA AHGVLRLVLE
110 120 130 140 150
LSGEMVRKCD PHIGLLHRGT EKLIEYKTYL QALPYFDRLD YVSMMCNEQA
160 170 180 190 200
YSLAVEKLLN IQPPPRAQWI RVLFGEITRI LNHIMAVTTH ALDIGAMTPF
210 220 230 240 250
FWMFEEREKM FEFYERVSGA RMHAAYIRPG GVHQDLPLGL MDDIYEFSKN
260 270 280 290 300
FSLRIDEVEE MLTNNRIWRN RTVDIGVVSA EDALNYGFSG VMLRGSGIQW
310 320 330 340 350
DLRKTQPYDV YDQVEFDVPI GSRGDCYDRY LCRVEEMRQS LRIIEQCLNK
360 370 380 390 400
MPPGEIKVDD AKVSPPKRAE MKTSMESLIH HFKLYTEGYQ VPPGATYTAI
410 420 430 440 450
EAPKGEFGVY LVSDGSSRPY RCKIKAPGFA HLAGLDKMSK GHMLADVVAI
460
IGTQDIVFGE IDR
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | BC082067 mRNA Translation: AAH82067.1 |
RefSeqi | NP_001011907.1, NM_001011907.1 XP_003751325.1, XM_003751277.3 |
Genome annotation databases
Ensembli | ENSRNOT00000058423; ENSRNOP00000055224; ENSRNOG00000038372 |
GeneIDi | 289218 |
KEGGi | rno:289218 |
UCSCi | RGD:1307109, rat |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | BC082067 mRNA Translation: AAH82067.1 |
RefSeqi | NP_001011907.1, NM_001011907.1 XP_003751325.1, XM_003751277.3 |
3D structure databases
SMRi | Q641Y2 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 252831, 2 interactors |
STRINGi | 10116.ENSRNOP00000055224 |
PTM databases
iPTMneti | Q641Y2 |
PhosphoSitePlusi | Q641Y2 |
2D gel databases
World-2DPAGEi | 0004:Q641Y2 |
Proteomic databases
jPOSTi | Q641Y2 |
PaxDbi | Q641Y2 |
PRIDEi | Q641Y2 |
Genome annotation databases
Ensembli | ENSRNOT00000058423; ENSRNOP00000055224; ENSRNOG00000038372 |
GeneIDi | 289218 |
KEGGi | rno:289218 |
UCSCi | RGD:1307109, rat |
Organism-specific databases
CTDi | 4720 |
RGDi | 1307109, Ndufs2 |
Phylogenomic databases
eggNOGi | KOG2870, Eukaryota |
GeneTreei | ENSGT00390000009529 |
HOGENOMi | CLU_015134_1_1_1 |
InParanoidi | Q641Y2 |
OMAi | IMGTSME |
OrthoDBi | 444312at2759 |
PhylomeDBi | Q641Y2 |
Enzyme and pathway databases
Reactomei | R-RNO-611105, Respiratory electron transport R-RNO-6799198, Complex I biogenesis |
Miscellaneous databases
PROi | PR:Q641Y2 |
Gene expression databases
Bgeei | ENSRNOG00000038372, Expressed in heart and 21 other tissues |
Genevisiblei | Q641Y2, RN |
Family and domain databases
Gene3Di | 1.10.645.20, 1 hit |
HAMAPi | MF_01358, NDH1_NuoD, 1 hit |
InterProi | View protein in InterPro IPR001135, NADH_Q_OxRdtase_suD IPR038290, NADH_Q_OxRdtase_suD_sf IPR014029, NADH_UbQ_OxRdtase_49kDa_CS IPR022885, NDH1_su_D/H IPR029014, NiFe-Hase_large |
Pfami | View protein in Pfam PF00346, Complex1_49kDa, 1 hit |
SUPFAMi | SSF56762, SSF56762, 1 hit |
TIGRFAMsi | TIGR01962, NuoD, 1 hit |
PROSITEi | View protein in PROSITE PS00535, COMPLEX1_49K, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | NDUS2_RAT | |
Accessioni | Q641Y2Primary (citable) accession number: Q641Y2 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 1, 2008 |
Last sequence update: | October 25, 2004 | |
Last modified: | February 10, 2021 | |
This is version 113 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families