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Protein

Transcription factor Dp-2

Gene

Tfdp2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Can stimulate E2F-dependent transcription. Binds DNA cooperatively with E2F family members through the E2 recognition site, 5'-TTTC[CG]CGC-3', found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The TFDP2:E2F complex functions in the control of cell-cycle progression from G1 to S phase. The E2F1:DP complex appears to mediate both cell proliferation and apoptosis. Blocks adipocyte differentiation by repressing CEBPA binding to its target gene promoters.By similarity

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi129 – 210Sequence analysisAdd BLAST82

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionActivator, DNA-binding
Biological processCell cycle, Transcription, Transcription regulation

Names & Taxonomyi

Protein namesi
Recommended name:
Transcription factor Dp-2
Alternative name(s):
Dp-3
E2F dimerization partner 2
Gene namesi
Name:Tfdp2
Synonyms:Dp2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:107167 Tfdp2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00002194782 – 446Transcription factor Dp-2Add BLAST445

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylthreonineBy similarity1
Modified residuei24Phosphoserine; by CDK2Sequence analysisBy similarity1
Modified residuei42Phosphoserine; by CDK2Sequence analysis1
Modified residuei122PhosphoserineBy similarity1

Post-translational modificationi

Phosphorylation by E2F1-bound cyclin A-CDK2, in the S phase, inhibits E2F-mediated DNA binding and transactivation.

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiQ64163
PeptideAtlasiQ64163
PRIDEiQ64163

PTM databases

PhosphoSitePlusiQ64163

Expressioni

Tissue specificityi

Expressed in all tissues examined. Highest levels in spleen and heart.

Gene expression databases

CleanExiMM_TFDP2

Interactioni

Subunit structurei

Component of the DRTF1/E2F transcription factor complex. Forms heterodimers with E2F family members. The complex can interact with hypophosphorylated retinoblastoma protein RB1 and related proteins (RBL1 and RBL2) that inhibit the E2F transactivation domain. During the cell cycle, RB becomes phosphorylated in mid-to-late G1 phase, detaches from the DRTF1/E2F complex rendering E2F transcriptionally active. Interacts with GMCL (By similarity). Component of the DREAM complex (also named LINC complex) at least composed of E2F4, E2F5, LIN9, LIN37, LIN52, LIN54, MYBL1, MYBL2, RBL1, RBL2, RBBP4, TFDP1 and TFDP2. The complex exists in quiescent cells where it represses cell cycle-dependent genes. It dissociates in S phase when LIN9, LIN37, LIN52 and LIN54 form a subcomplex that binds to MYBL2. The complex TFDP2:E2F1 interacts with CEBPA; the interaction prevents CEBPA binding to target gene promoters and represses its transcriptional activity (By similarity).By similarity1 Publication

Binary interactionsi

GO - Molecular functioni

Protein-protein interaction databases

DIPiDIP-24228N
IntActiQ64163, 3 interactors
STRINGi10090.ENSMUSP00000128260

Structurei

3D structure databases

ProteinModelPortaliQ64163
SMRiQ64163
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni60 – 82Interaction with CEBPABy similarityAdd BLAST23
Regioni219 – 292DimerizationSequence analysisAdd BLAST74
Regioni229 – 261DCB1Add BLAST33
Regioni274 – 330DCB2Add BLAST57

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi103 – 118Nuclear localization signal1 PublicationAdd BLAST16
Motifi176 – 210DEF boxAdd BLAST35

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi432 – 446Asp/Glu-rich (acidic; NCB domain)Add BLAST15

Sequence similaritiesi

Belongs to the E2F/DP family.Curated

Phylogenomic databases

eggNOGiKOG2829 Eukaryota
ENOG410Y9QP LUCA
HOGENOMiHOG000030696
HOVERGENiHBG009894
InParanoidiQ64163
PhylomeDBiQ64163

Family and domain databases

CDDicd14458 DP_DD, 1 hit
Gene3Di1.10.10.10, 1 hit
1.20.140.80, 1 hit
InterProiView protein in InterPro
IPR028314 DP-2
IPR037241 E2F-DP_heterodim
IPR003316 E2F_WHTH_DNA-bd_dom
IPR038168 TF_DP_C_sf
IPR014889 Transc_factor_DP_C
IPR015648 Transcrpt_fac_DP
IPR036388 WH-like_DNA-bd_sf
IPR036390 WH_DNA-bd_sf
PANTHERiPTHR12548 PTHR12548, 1 hit
PTHR12548:SF5 PTHR12548:SF5, 1 hit
PfamiView protein in Pfam
PF08781 DP, 1 hit
PF02319 E2F_TDP, 1 hit
PIRSFiPIRSF009404 Transcription_factor_DP, 1 hit
SMARTiView protein in SMART
SM01138 DP, 1 hit
SM01372 E2F_TDP, 1 hit
SUPFAMiSSF144074 SSF144074, 1 hit
SSF46785 SSF46785, 1 hit

Sequences (4+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 4 isoformsi produced by alternative splicing. AlignAdd to basket
Note: Additional isoforms seem to exist.

This entry has 4 described isoforms and 11 potential isoforms that are computationally mapped.Show allAlign All

Isoform Alpha (identifier: Q64163-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MTAKNVGLPS TNAKLRGFID QNFSPSKGNI SLVAFPVSST NSPTKILPKT
60 70 80 90 100
LGPINVNVGP QMIISTPQRI ANSGSVLIGN PYTPAPAMVT QTHIAEAAGW
110 120 130 140 150
VPSDRKRARE FIDSDFSESK RSKKGDKNGK GLRHFSMKVC EKVQRKGTTS
160 170 180 190 200
YNEVADELVS EFTNSNNHLA ADSAYDQENI RRRVYDALNV LMAMNIISSL
210 220 230 240 250
PTGKKRNQVD CNSAQECQNL EIEKQRRIER IKQKRAQLQE LLLQQIAFKN
260 270 280 290 300
LVQRNRQNEQ QNQGPPAVNS TIQLPFIIIN TSRKTVIDCS ISSDKFEYLF
310 320 330 340 350
NFDNTFEIHD DIEVLKRMGM SFGLESGKCS LEDLKIARSL VPKALEGYIT
360 370 380 390 400
DISTGPSWLN QGLLLNSTQS VSNLDPTTGA TVPQSSVNQG LCLDAEVALA
410 420 430 440
TGQLPASNSH QSSSAASHFS ESRGETPCSF NDEDEEDEEE DPSSPE
Length:446
Mass (Da):49,098
Last modified:May 30, 2000 - v2
Checksum:i0E6E87AE8B82EE8A
GO
Isoform Beta (identifier: Q64163-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-61: Missing.
     103-118: Missing.

Show »
Length:369
Mass (Da):40,811
Checksum:i3C198019055FB478
GO
Isoform Gamma (identifier: Q64163-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-61: Missing.
     103-118: Missing.
     173-173: S → SQ

Show »
Length:370
Mass (Da):40,939
Checksum:iB76BC25F93256944
GO
Isoform Delta (identifier: Q64163-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-61: Missing.

Show »
Length:385
Mass (Da):42,751
Checksum:iC76E95880BB3582E
GO

Computationally mapped potential isoform sequencesi

There are 11 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
F6QG91F6QG91_MOUSE
Transcription factor
Tfdp2
386Annotation score:
E9PWL5E9PWL5_MOUSE
Transcription factor
Tfdp2
310Annotation score:
J3QK26J3QK26_MOUSE
Transcription factor
Tfdp2
359Annotation score:
Q8BHD2Q8BHD2_MOUSE
Transcription factor
Tfdp2
385Annotation score:
A0A087WPU8A0A087WPU8_MOUSE
Transcription factor
Tfdp2
446Annotation score:
A0A087WPS0A0A087WPS0_MOUSE
Transcription factor Dp-2
Tfdp2
203Annotation score:
A0A087WSK4A0A087WSK4_MOUSE
Transcription factor Dp-2
Tfdp2
369Annotation score:
A0A1B0GXK0A0A1B0GXK0_MOUSE
Transcription factor Dp-2
Tfdp2
368Annotation score:
A0A087WQV5A0A087WQV5_MOUSE
Transcription factor Dp-2
Tfdp2
112Annotation score:
A0A087WRW3A0A087WRW3_MOUSE
Transcription factor Dp-2
Tfdp2
39Annotation score:
There is more potential isoformShow all

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0013551 – 61Missing in isoform Beta, isoform Gamma and isoform Delta. 1 PublicationAdd BLAST61
Alternative sequenceiVSP_001356103 – 118Missing in isoform Beta and isoform Gamma. 1 PublicationAdd BLAST16
Alternative sequenceiVSP_001357173S → SQ in isoform Gamma. 1 Publication1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S79780 mRNA Translation: AAB35506.2
CCDSiCCDS23413.1 [Q64163-4]
UniGeneiMm.17977
Mm.447855

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S79780 mRNA Translation: AAB35506.2
CCDSiCCDS23413.1 [Q64163-4]
UniGeneiMm.17977
Mm.447855

3D structure databases

ProteinModelPortaliQ64163
SMRiQ64163
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-24228N
IntActiQ64163, 3 interactors
STRINGi10090.ENSMUSP00000128260

PTM databases

PhosphoSitePlusiQ64163

Proteomic databases

PaxDbiQ64163
PeptideAtlasiQ64163
PRIDEiQ64163

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Organism-specific databases

MGIiMGI:107167 Tfdp2

Phylogenomic databases

eggNOGiKOG2829 Eukaryota
ENOG410Y9QP LUCA
HOGENOMiHOG000030696
HOVERGENiHBG009894
InParanoidiQ64163
PhylomeDBiQ64163

Miscellaneous databases

ChiTaRSiTfdp2 mouse
PROiPR:Q64163
SOURCEiSearch...

Gene expression databases

CleanExiMM_TFDP2

Family and domain databases

CDDicd14458 DP_DD, 1 hit
Gene3Di1.10.10.10, 1 hit
1.20.140.80, 1 hit
InterProiView protein in InterPro
IPR028314 DP-2
IPR037241 E2F-DP_heterodim
IPR003316 E2F_WHTH_DNA-bd_dom
IPR038168 TF_DP_C_sf
IPR014889 Transc_factor_DP_C
IPR015648 Transcrpt_fac_DP
IPR036388 WH-like_DNA-bd_sf
IPR036390 WH_DNA-bd_sf
PANTHERiPTHR12548 PTHR12548, 1 hit
PTHR12548:SF5 PTHR12548:SF5, 1 hit
PfamiView protein in Pfam
PF08781 DP, 1 hit
PF02319 E2F_TDP, 1 hit
PIRSFiPIRSF009404 Transcription_factor_DP, 1 hit
SMARTiView protein in SMART
SM01138 DP, 1 hit
SM01372 E2F_TDP, 1 hit
SUPFAMiSSF144074 SSF144074, 1 hit
SSF46785 SSF46785, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiTFDP2_MOUSE
AccessioniPrimary (citable) accession number: Q64163
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: May 30, 2000
Last modified: November 7, 2018
This is version 151 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
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Main funding by: National Institutes of Health

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