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Protein

T-cell surface glycoprotein CD3 gamma chain

Gene

Cd3g

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Part of the TCR-CD3 complex present on T-lymphocyte cell surface that plays an essential role in adaptive immune response. When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain. Upon TCR engagement, these motifs become phosphorylated by Src family protein tyrosine kinases LCK and FYN, resulting in the activation of downstream signaling pathways. In addition to this role of signal transduction in T-cell activation, CD3G plays an essential role in the dynamic regulation of TCR expression at the cell surface. Indeed, constitutive TCR cycling is dependent on the di-leucine-based (diL) receptor-sorting motif present in CD3G.By similarity

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionReceptor
Biological processAdaptive immunity, Immunity

Names & Taxonomyi

Protein namesi
Recommended name:
T-cell surface glycoprotein CD3 gamma chain
Alternative name(s):
T-cell receptor T3 gamma chain
CD_antigen: CD3g
Gene namesi
Name:Cd3g
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi1307718 Cd3g

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini23 – 116ExtracellularSequence analysisAdd BLAST94
Transmembranei117 – 137HelicalSequence analysisAdd BLAST21
Topological domaini138 – 182CytoplasmicSequence analysisAdd BLAST45

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 22By similarityAdd BLAST22
ChainiPRO_000001461823 – 182T-cell surface glycoprotein CD3 gamma chainAdd BLAST160

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi46 ↔ 87By similarity
Glycosylationi66N-linked (GlcNAc...) asparagineSequence analysis1
Modified residuei145PhosphoserineBy similarity1
Modified residuei148Phosphoserine; by PKCBy similarity1

Post-translational modificationi

Phosphorylated on Tyr residues after T-cell receptor triggering by LCK in association with CD4/CD8. Phosphorylated also by PKC; leading to the TCR complex down-regulation.By similarity
Phosphorylated on Tyr residues after T-cell receptor triggering by LCK in association with CD4/CD8.By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiQ64159
PRIDEiQ64159

Interactioni

Subunit structurei

The TCR-CD3 complex is composed of a CD3D/CD3E and a CD3G/CD3E heterodimers that preferentially associate with TCRalpha and TCRbeta, respectively, to form TCRalpha/CD3E/CD3G and TCRbeta/CD3G/CD3E trimers. In turn, the hexamer interacts with CD3Z homodimer to form the TCR-CD3 complex. Alternatively, TCRalpha and TCRbeta can be replaced by TCRgamma and TCRdelta.By similarity

GO - Molecular functioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000021390

Structurei

3D structure databases

ProteinModelPortaliQ64159
SMRiQ64159
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini37 – 94Ig-likeAdd BLAST58
Domaini149 – 177ITAMPROSITE-ProRule annotationAdd BLAST29

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi153 – 154Di-leucine motifBy similarity2

Domaini

A di-leucine motif and a tyrosine-based motif are individually sufficient to induce both endocytosis and delivery to lysosomes.By similarity

Keywords - Domaini

Immunoglobulin domain, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IWAX Eukaryota
ENOG410Y2TD LUCA
HOGENOMiHOG000015287
HOVERGENiHBG005278
InParanoidiQ64159
KOiK06452
PhylomeDBiQ64159

Family and domain databases

Gene3Di2.60.40.10, 1 hit
InterProiView protein in InterPro
IPR015484 CD3_esu/gsu/dsu
IPR036179 Ig-like_dom_sf
IPR013783 Ig-like_fold
IPR032052 Ig_4
IPR003598 Ig_sub2
IPR003110 Phos_immunorcpt_sig_ITAM
PANTHERiPTHR10570 PTHR10570, 1 hit
PfamiView protein in Pfam
PF16680 Ig_4, 1 hit
PF02189 ITAM, 1 hit
SMARTiView protein in SMART
SM00408 IGc2, 1 hit
SUPFAMiSSF48726 SSF48726, 1 hit
PROSITEiView protein in PROSITE
PS51055 ITAM_1, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry has 1 described isoform and 1 potential isoform that is computationally mapped.iShow all

Q64159-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MEQGKGLAGL FLVISLLQGT MAQQKEEKHL VKVDDSQGDG SVLLTCDFNE
60 70 80 90 100
KTITWLKDGH RISPPNATKS TWNLGNGAKD PRGMYQCRGA KKKSQLLQVY
110 120 130 140 150
YRLCENCIEL NMGTVSGFIF AEIISIFFLA VGVYFIAGQD GVRQSRASDK
160 170 180
QTLLQNEQVY QPLKDREYEQ YSRLQGNQVR KK
Length:182
Mass (Da):20,545
Last modified:November 1, 1997 - v1
Checksum:iA4DBA7AA67FACED7
GO

Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
F1M9F8F1M9F8_RAT
T-cell surface glycoprotein CD3 gam...
Cd3g
182Annotation score:

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S79711 mRNA Translation: AAB21286.2
RefSeqiNP_001071114.1, NM_001077646.2

Genome annotation databases

GeneIDi300678
KEGGirno:300678
UCSCiRGD:1307718 rat

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S79711 mRNA Translation: AAB21286.2
RefSeqiNP_001071114.1, NM_001077646.2

3D structure databases

ProteinModelPortaliQ64159
SMRiQ64159
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000021390

Proteomic databases

PaxDbiQ64159
PRIDEiQ64159

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi300678
KEGGirno:300678
UCSCiRGD:1307718 rat

Organism-specific databases

CTDi917
RGDi1307718 Cd3g

Phylogenomic databases

eggNOGiENOG410IWAX Eukaryota
ENOG410Y2TD LUCA
HOGENOMiHOG000015287
HOVERGENiHBG005278
InParanoidiQ64159
KOiK06452
PhylomeDBiQ64159

Miscellaneous databases

PROiPR:Q64159

Family and domain databases

Gene3Di2.60.40.10, 1 hit
InterProiView protein in InterPro
IPR015484 CD3_esu/gsu/dsu
IPR036179 Ig-like_dom_sf
IPR013783 Ig-like_fold
IPR032052 Ig_4
IPR003598 Ig_sub2
IPR003110 Phos_immunorcpt_sig_ITAM
PANTHERiPTHR10570 PTHR10570, 1 hit
PfamiView protein in Pfam
PF16680 Ig_4, 1 hit
PF02189 ITAM, 1 hit
SMARTiView protein in SMART
SM00408 IGc2, 1 hit
SUPFAMiSSF48726 SSF48726, 1 hit
PROSITEiView protein in PROSITE
PS51055 ITAM_1, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiCD3G_RAT
AccessioniPrimary (citable) accession number: Q64159
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: May 23, 2018
This is version 120 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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