UniProtKB - Q64127 (TIF1A_MOUSE)

BLAST

>sp|Q64127|TIF1A_MOUSE Transcription intermediary factor 1-alpha OS=Mus musculus OX=10090 GN=Trim24 PE=1 SV=1
MEVAVEKAAAAAAPAGGPAAAAPSGENEAESRQGPDSESGGEASRLNLLDTCAVCHQNIQ
SRVPKLLPCLHSFCQRCLPAPQRYLMLTAPALGSAETPPPAPAPAPAPGSPAGGPSPFAT
QVGVIRCPVCSQECAERHIIDNFFVKDTTEVPSSTVEKSNQVCTSCEDNAEANGFCVECV
EWLCKTCIRAHQRVKFTKDHTVRQKEEVSPEAVGVTSQRPVFCPFHKKEQLKLYCETCDK
LTCRDCQLLEHKEHRYQFIEEAFQNQKVIIDTLITKLMEKTKYIKYTGNQIQNRIIEINQ
NQKQVEQDIKVAIFTLMVEINKKGKALLHQLESLAKDHRMKLMQQQQEVAGLSKQLEHVM
HFSKWAVSSGSSTALLYSKRLITYRLRHLLRARCDASPVTNTTIQFHCDPSFWAQNIINL
GSLVIEDKESQPQMPKQNPVVEQSSQPPGGLPSNQLSKFPTQISLAQLRLQHIQQQVMAQ
RQQVQRRPAPVGLPNPRMQGPIQQPSISHQHPPPRLINFQNHSPKPNGPVLPPYPQQLRY
SPSQNVPRQTTIKPNPLQMAFLAQQAIKQWQISSVQAPPTTASSSSSTPSSPTITSAAGY
DGKAFSSPMIDLSAPVGGSYNLPSLPDIDCSSTIMLDNIARKDTGVDHAQPRPPSNRTVQ
SPNSSVPSPGLAGPVTMTSVHPPIRSPSASSVGSRGSSGSSSKPAGADSTHKVPVVMLEP
IRIKQENSGPPENYDFPVVIVKQESDEESRPQNTNYPRSILTSLLLNSSQSSASEETVLR
SDAPDSTGDQPGLHQENSSNGKSEWSDASQKSPVHVGETRKEDDPNEDWCAVCQNGGELL
CCEKCPKVFHLTCHVPTLTNFPSGEWICTFCRDLSKPEVDYDCDVPSHHSEKRKSEGLTK
LTPIDKRKCERLLLFLYCHEMSLAFQDPVPLTVPDYYKIIKNPMDLSTIKKRLQEDYCMY
TKPEDFVADFRLIFQNCAEFNEPDSEVANAGIKLESYFEELLKNLYPEKRFPKVEFRHEA
EDCKFSDDSDDDFVQPRKKRLKSTEDRQLLK

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History
Entry version 177 (07 Nov 2018)
Sequence version 1 (01 Nov 1996)
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Protein

Transcription intermediary factor 1-alpha

Gene

Trim24

Organism

Mus musculus (Mouse)

Status

Reviewed-Annotation score: -Experimental evidence at protein levelⁱ

Functionⁱ

Transcriptional coactivator that interacts with numerous nuclear receptors and coactivators and modulates the transcription of target genes. Interacts with chromatin depending on histone H3 modifications, having the highest affinity for histone H3 that is both unmodified at 'Lys-4' (H3K4me0) and acetylated at 'Lys-23' (H3K23ac) (By similarity). Has E3 protein-ubiquitin ligase activity. Promotes ubiquitination and proteasomal degradation of p53/TP53. Plays a role in the regulation of cell proliferation and apoptosis via its effects on p53/TP53 levels. Up-regulates ligand-dependent transcription activation by AR, GCR/NR3C1, thyroid hormone receptor (TR) and ESR1. Modulates transcription activation by retinoic acid (RA) receptors, such as RARA. Plays a role in regulating retinoic acid-dependent proliferation of hepatocytes. Required for normal transition from proliferating neonatal hepatocytes to quiescent adult hepatocytes.By similarity7 Publications

Catalytic activityⁱ

S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N₆-ubiquitinyl-[acceptor protein]-L-lysine.

Pathwayⁱ: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

Regions

Feature key	Position(s)	DescriptionActions	Length
Zinc fingerⁱ	52 – 77	RING-typePROSITE-ProRule annotationAdd BLAST	26
Zinc fingerⁱ	158 – 211	B box-type 1PROSITE-ProRule annotationAdd BLAST	54
Zinc fingerⁱ	218 – 259	B box-type 2PROSITE-ProRule annotationAdd BLAST	42
Zinc fingerⁱ	827 – 874	PHD-typePROSITE-ProRule annotationAdd BLAST	48

GO - Molecular functionⁱ

chromatin binding
Source: MGI
Inferred from direct assayⁱ
- 10318760
estrogen response element binding Source: UniProtKB
lysine-acetylated histone binding Source: UniProtKB
nuclear receptor binding
Source: MGI
Inferred from direct assayⁱ
- 15322135
p53 binding
Source: UniProtKB
Inferred from physical interactionⁱ
- 19556538
protein kinase activity
Source: MGI
Inferred from direct assayⁱ
- 10562550
sequence-specific DNA binding
Source: MGI
Inferred from direct assayⁱ
- 18026104
transcription coactivator activity
Source: UniProtKB
Inferred from direct assayⁱ
- 19909775
ubiquitin protein ligase activity
Source: MGI
Inferred from direct assayⁱ
- 19556538
ubiquitin-protein transferase activity
Source: UniProtKB
Inferred from direct assayⁱ
- 19556538
zinc ion binding Source: UniProtKB

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

calcium ion homeostasis
Source: MGI
Inferred from mutant phenotypeⁱ
- 18287084
cellular response to estrogen stimulus Source: UniProtKB
negative regulation of cell proliferation
Source: MGI
Inferred from mutant phenotypeⁱ
- 18026104
negative regulation of transcription, DNA-templated
Source: MGI
Inferred from direct assayⁱ
- 10562550
- 18026104
positive regulation of gene expression
Source: MGI
Inferred from mutant phenotypeⁱ
- 18026104
positive regulation of transcription, DNA-templated
Source: MGI
Inferred from genetic interactionⁱ
- 7744009
protein autophosphorylation
Source: MGI
Inferred from direct assayⁱ
- 10562550
protein catabolic process
Source: UniProtKB
Inferred from mutant phenotypeⁱ
- 19556538
protein phosphorylation
Source: MGI
Inferred from direct assayⁱ
- 10562550
protein ubiquitination
Source: UniProtKB
Inferred from direct assayⁱ
- 19556538
regulation of apoptotic process Source: MGI
regulation of protein stability
Source: UniProtKB
Inferred from mutant phenotypeⁱ
- 19556538
regulation of signal transduction by p53 class mediator
Source: MGI
Inferred from genetic interactionⁱ
- 19556538
regulation of vitamin D receptor signaling pathway
Source: MGI
Inferred from mutant phenotypeⁱ
- 18287084

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Molecular function	DNA-binding, Repressor, Transferase
Biological process	Transcription, Transcription regulation, Ubl conjugation pathway
Ligand	Metal-binding, Zinc

Enzyme and pathway databases

UniPathwayⁱ	UPA00143

UniPathwayⁱ

UPA00143

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: Transcription intermediary factor 1-alpha (EC:2.3.2.27) Short name: TIF1-alpha Alternative name(s): E3 ubiquitin-protein ligase Trim24 RING-type E3 ubiquitin transferase TIF1-alphaCurated Tripartite motif-containing protein 24
Gene namesⁱ	Name:Trim24 Synonyms:Tif1, Tif1a
Organismⁱ	Mus musculus (Mouse)
Taxonomic identifierⁱ	10090 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Dipnotetrapodomorpha › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Boreoeutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus
Proteomesⁱ	UP000000589 Componentⁱ: Chromosome 6

Organism-specific databases

MGIⁱ	MGI:109275 Trim24

Keywords - Cellular componentⁱ

Cytoplasm, Nucleus

Pathology & Biotechⁱ

Involvement in diseaseⁱ

A chromosomal aberration involving TRIM24 produces a TRIM24-BRAF (T18) oncogene originally isolated from a furfural-induced hepatoma.

Disruption phenotypeⁱ

No visible phenotype during the first few months. Impaired transition from proliferating neonatal hepatocytes to quiescent adult hepatocytes. Hepatocytes continue to proliferate throughout adulthood. High incidence hypertrophic hepatocytes with enlarged nuclei after three months. After nine months, about half of the mice have hepatocellular adenomas. Very high incidence of hepatocarcinoma in 13 to 29 month old mice, increasing from 40% to 80%. When one copy of Rara is disrupted, mice do not develop liver tumors or liver dysplasia.2 Publications

Mutagenesis

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Mutagenesisⁱ	724	K → R: Loss of sumoylation; when associated with R-742. 1 Publication		1
Mutagenesisⁱ	742	K → R: Loss of sumoylation; when associated with R-724. 1 Publication		1

Sites

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Siteⁱ	332 – 333	Breakpoint for translocation to form TRIM24-BRAF oncogene		2

Keywords - Diseaseⁱ

Proto-oncogene, Tumor suppressor

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
ChainⁱPRO_0000056391	1 – 1051	Transcription intermediary factor 1-alphaAdd BLAST		1051

Amino acid modifications

Feature key	Position(s)	DescriptionActions	Length
Cross-linkⁱ	7	Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residueⁱ	97	PhosphothreonineBy similarity	1
Modified residueⁱ	110	PhosphoserineBy similarity	1
Cross-linkⁱ	205	Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linkⁱ	276	Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linkⁱ	436	Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linkⁱ	458	Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residueⁱ	469	Omega-N-methylarginineCombined sources	1
Cross-linkⁱ	553	Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linkⁱ	642	Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residueⁱ	655	PhosphoserineBy similarity	1
Modified residueⁱ	661	PhosphoserineCombined sources	1
Modified residueⁱ	668	PhosphoserineCombined sources	1
Cross-linkⁱ	703	Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linkⁱ	712	Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linkⁱ	724	Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO); alternateCurated
Cross-linkⁱ	724	Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO1); alternateBy similarity
Cross-linkⁱ	724	Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2); alternateBy similarity
Cross-linkⁱ	742	Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO); alternateCurated
Cross-linkⁱ	742	Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2); alternateBy similarity
Modified residueⁱ	745	PhosphoserineBy similarity	1
Modified residueⁱ	769	PhosphoserineBy similarity	1
Cross-linkⁱ	802	Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residueⁱ	809	PhosphoserineBy similarity	1
Cross-linkⁱ	811	Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residueⁱ	812	PhosphoserineCombined sources	1
Modified residueⁱ	819	PhosphothreonineBy similarity	1
Cross-linkⁱ	876	Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linkⁱ	950	Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linkⁱ	993	Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residueⁱ	1026	PhosphoserineCombined sources	1
Modified residueⁱ	1029	PhosphoserineBy similarity	1
Cross-linkⁱ	1042	Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residueⁱ	1043	PhosphoserineBy similarity	1

Post-translational modificationⁱ

Sumoylated.1 Publication

Keywords - PTMⁱ

Isopeptide bond, Methylation, Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbⁱ	Q64127
PeptideAtlas More...PeptideAtlasⁱ	Q64127
PRIDEⁱ	Q64127

PTM databases

iPTMnetⁱ	Q64127
PhosphoSitePlusⁱ	Q64127

Expressionⁱ

Tissue specificityⁱ

Detected in embryonic and adult liver. Detected in zygote and throughout embryogenesis (at protein level). Detected in all adult tissues, with the highest expression level in testis.2 Publications

Inductionⁱ

Before puberty, highly expressed in liver from males and females. After puberty, expression is considerably higher in liver from females compared to males. Up-regulated in males by continuous exposure to growth hormone.1 Publication

Gene expression databases

Bgeeⁱ	ENSMUSG00000029833 Expressed in 308 organ(s), highest expression level in testis
CleanExⁱ	MM_TRIM24
ExpressionAtlasⁱ	Q64127 baseline and differential
Genevisibleⁱ	Q64127 MM

Interactionⁱ

Subunit structureⁱ

Interacts (via bromo domain) with histone H3 (via N-terminus), provided that it is not methylated at 'Lys-4' (H3K4me0). Does not interact with histone H3 that is methylated at 'Lys-4' (H3K4me1, H3K4me2 or H3K4me3). Interacts (via bromo domain) with histone H3 (via N-terminus) that is acetylated at 'Lys-23' (H3K23ac). Has the highest affinity for histone H3 that is both unmodified at 'Lys-4' (H3K4me0) and acetylated at 'Lys-23' (H3K23ac). Has very low affinity for histone H3 that is methylated at 'Lys-9' (H3K9me), or acetylated at both 'Lys-9' (H3K9ac) and 'Lys-14' (H3K14ac), or acetylated at 'Lys-27' (H3K27ac) (in vitro). Interacts with TRIM16. Interacts with NR3C2/MCR (By similarity). Interacts with the ligand-binding domain of estrogen receptors (in vitro). Interaction with DNA-bound estrogen receptors requires the presence of estradiol (By similarity). Interacts with AR, CARM1, KAT5/TIP60, NCOA2/GRIP1, BRD7, CBX1, CBX3 and CBX5. Part of a coactivator complex containing TRIM24, NCOA2/GRIP1 and CARM1. Interacts with p53/TP53 and PML.By similarity7 Publications

Sites

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Siteⁱ	828	Interaction with histone H3 that is not methylated at 'Lys-4' (H3K4me0)By similarity		1

Binary interactionsⁱ

With	Entry	#Exp.	IntAct
Cbx1	P83917	4	EBI-307947,EBI-78119
Cbx5	Q61686	4	EBI-307947,EBI-307973
Tp53	P02340	2	EBI-307947,EBI-474016
Trim33	Q99PP7	2	EBI-307947,EBI-3043980

GO - Molecular functionⁱ

lysine-acetylated histone binding Source: UniProtKB
nuclear receptor binding
Source: MGI
Inferred from direct assayⁱ
- 15322135
p53 binding
Source: UniProtKB
Inferred from physical interactionⁱ
- 19556538

View the complete GO annotation on QuickGO ...

Protein-protein interaction databases

BioGridⁱ	204196, 11 interactors
Database of interacting proteins More...DIPⁱ	DIP-31476N
IntActⁱ	Q64127, 13 interactors
Molecular INTeraction database More...MINTⁱ	Q64127
STRINGⁱ	10090.ENSMUSP00000031859

Structureⁱ

3D structure databases

ProteinModelPortalⁱ	Q64127
SMRⁱ	Q64127
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Family & Domainsⁱ

Domains and Repeats

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Domainⁱ	933 – 988	BromoPROSITE-ProRule annotationAdd BLAST		56

Region

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Regionⁱ	755 – 780	Nuclear receptor binding site (NRBS)Add BLAST		26
Regionⁱ	835 – 841	Interaction with histone H3 that is not methylated at 'Lys-4' (H3K4me0)By similarity		7

Coiled coil

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Coiled coilⁱ	289 – 359	Sequence analysisAdd BLAST		71

Motif

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Motifⁱ	892 – 908	Nuclear localization signalSequence analysisAdd BLAST		17

Compositional bias

Feature key	Position(s)	DescriptionActions	Length
Compositional biasⁱ	8 – 15	Poly-Ala	8
Compositional biasⁱ	19 – 22	Poly-Ala	4
Compositional biasⁱ	344 – 347	Poly-Gln	4
Compositional biasⁱ	583 – 587	Poly-Ser	5

Zinc finger

Feature key	Position(s)	DescriptionActions	Length
Zinc fingerⁱ	52 – 77	RING-typePROSITE-ProRule annotationAdd BLAST	26
Zinc fingerⁱ	158 – 211	B box-type 1PROSITE-ProRule annotationAdd BLAST	54
Zinc fingerⁱ	218 – 259	B box-type 2PROSITE-ProRule annotationAdd BLAST	42
Zinc fingerⁱ	827 – 874	PHD-typePROSITE-ProRule annotationAdd BLAST	48

Keywords - Domainⁱ

Bromodomain, Coiled coil, Repeat, Zinc-finger

Phylogenomic databases

eggNOGⁱ	ENOG410ITFE Eukaryota COG5076 LUCA
Ensembl GeneTree More...GeneTreeⁱ	ENSGT00920000148978
HOGENOMⁱ	HOG000252971
HOVERGENⁱ	HBG054599
InParanoidⁱ	Q64127
KEGG Orthology (KO) More...KOⁱ	K08881
OMAⁱ	FWAQNIF
Database of Orthologous Groups More...OrthoDBⁱ	EOG091G01KK
PhylomeDBⁱ	Q64127
TreeFamⁱ	TF106455

Family and domain databases

Conserved Domains Database More...CDDⁱ	cd00021 BBOX, 1 hit
Gene3Dⁱ	1.20.920.10, 1 hit
InterProⁱ	View protein in InterPro IPR003649 Bbox_C IPR001487 Bromodomain IPR036427 Bromodomain-like_sf IPR018359 Bromodomain_CS IPR019786 Zinc_finger_PHD-type_CS IPR000315 Znf_B-box IPR011011 Znf_FYVE_PHD IPR001965 Znf_PHD IPR019787 Znf_PHD-finger IPR001841 Znf_RING IPR017907 Znf_RING_CS
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF00439 Bromodomain, 1 hit PF00628 PHD, 1 hit PF00643 zf-B_box, 1 hit
PRINTSⁱ	PR00503 BROMODOMAIN
SMARTⁱ	View protein in SMART SM00502 BBC, 1 hit SM00336 BBOX, 2 hits SM00297 BROMO, 1 hit SM00249 PHD, 1 hit SM00184 RING, 1 hit
SUPFAMⁱ	SSF47370 SSF47370, 1 hit SSF57903 SSF57903, 1 hit
PROSITEⁱ	View protein in PROSITE PS00633 BROMODOMAIN_1, 1 hit PS50014 BROMODOMAIN_2, 1 hit PS50119 ZF_BBOX, 2 hits PS01359 ZF_PHD_1, 1 hit PS50016 ZF_PHD_2, 1 hit PS00518 ZF_RING_1, 1 hit PS50089 ZF_RING_2, 1 hit

Sequences (2+)ⁱ

Sequence statusⁱ: Complete.

This entry describes 2 isoformsⁱ produced by alternative splicing. Align Add to basket

This entry has 2 described isoforms and 1 potential isoform that is computationally mapped.Show all Align All

Isoform Long (identifier: Q64127-1) [UniParc]FASTA Add to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MEVAVEKAAA AAAPAGGPAA AAPSGENEAE SRQGPDSESG GEASRLNLLD 
        60         70         80         90        100
TCAVCHQNIQ SRVPKLLPCL HSFCQRCLPA PQRYLMLTAP ALGSAETPPP 
       110        120        130        140        150
APAPAPAPGS PAGGPSPFAT QVGVIRCPVC SQECAERHII DNFFVKDTTE 
       160        170        180        190        200
VPSSTVEKSN QVCTSCEDNA EANGFCVECV EWLCKTCIRA HQRVKFTKDH 
       210        220        230        240        250
TVRQKEEVSP EAVGVTSQRP VFCPFHKKEQ LKLYCETCDK LTCRDCQLLE 
       260        270        280        290        300
HKEHRYQFIE EAFQNQKVII DTLITKLMEK TKYIKYTGNQ IQNRIIEINQ 
       310        320        330        340        350
NQKQVEQDIK VAIFTLMVEI NKKGKALLHQ LESLAKDHRM KLMQQQQEVA 
       360        370        380        390        400
GLSKQLEHVM HFSKWAVSSG SSTALLYSKR LITYRLRHLL RARCDASPVT 
       410        420        430        440        450
NTTIQFHCDP SFWAQNIINL GSLVIEDKES QPQMPKQNPV VEQSSQPPGG 
       460        470        480        490        500
LPSNQLSKFP TQISLAQLRL QHIQQQVMAQ RQQVQRRPAP VGLPNPRMQG 
       510        520        530        540        550
PIQQPSISHQ HPPPRLINFQ NHSPKPNGPV LPPYPQQLRY SPSQNVPRQT 
       560        570        580        590        600
TIKPNPLQMA FLAQQAIKQW QISSVQAPPT TASSSSSTPS SPTITSAAGY 
       610        620        630        640        650
DGKAFSSPMI DLSAPVGGSY NLPSLPDIDC SSTIMLDNIA RKDTGVDHAQ 
       660        670        680        690        700
PRPPSNRTVQ SPNSSVPSPG LAGPVTMTSV HPPIRSPSAS SVGSRGSSGS 
       710        720        730        740        750
SSKPAGADST HKVPVVMLEP IRIKQENSGP PENYDFPVVI VKQESDEESR 
       760        770        780        790        800
PQNTNYPRSI LTSLLLNSSQ SSASEETVLR SDAPDSTGDQ PGLHQENSSN 
       810        820        830        840        850
GKSEWSDASQ KSPVHVGETR KEDDPNEDWC AVCQNGGELL CCEKCPKVFH 
       860        870        880        890        900
LTCHVPTLTN FPSGEWICTF CRDLSKPEVD YDCDVPSHHS EKRKSEGLTK 
       910        920        930        940        950
LTPIDKRKCE RLLLFLYCHE MSLAFQDPVP LTVPDYYKII KNPMDLSTIK 
       960        970        980        990       1000
KRLQEDYCMY TKPEDFVADF RLIFQNCAEF NEPDSEVANA GIKLESYFEE 
      1010       1020       1030       1040       1050
LLKNLYPEKR FPKVEFRHEA EDCKFSDDSD DDFVQPRKKR LKSTEDRQLL 

K

Length:1,051

Mass (Da):116,657

Last modified:November 1, 1996 - v1

Checksum:ⁱ610584C1C6885972

Isoform Short (identifier: Q64127-2) [UniParc]FASTA Add to basket

The sequence of this isoform differs from the canonical sequence as follows:
477-510: Missing.

« Hide

        10         20         30         40         50
MEVAVEKAAA AAAPAGGPAA AAPSGENEAE SRQGPDSESG GEASRLNLLD 
        60         70         80         90        100
TCAVCHQNIQ SRVPKLLPCL HSFCQRCLPA PQRYLMLTAP ALGSAETPPP 
       110        120        130        140        150
APAPAPAPGS PAGGPSPFAT QVGVIRCPVC SQECAERHII DNFFVKDTTE 
       160        170        180        190        200
VPSSTVEKSN QVCTSCEDNA EANGFCVECV EWLCKTCIRA HQRVKFTKDH 
       210        220        230        240        250
TVRQKEEVSP EAVGVTSQRP VFCPFHKKEQ LKLYCETCDK LTCRDCQLLE 
       260        270        280        290        300
HKEHRYQFIE EAFQNQKVII DTLITKLMEK TKYIKYTGNQ IQNRIIEINQ 
       310        320        330        340        350
NQKQVEQDIK VAIFTLMVEI NKKGKALLHQ LESLAKDHRM KLMQQQQEVA 
       360        370        380        390        400
GLSKQLEHVM HFSKWAVSSG SSTALLYSKR LITYRLRHLL RARCDASPVT 
       410        420        430        440        450
NTTIQFHCDP SFWAQNIINL GSLVIEDKES QPQMPKQNPV VEQSSQPPGG 
       460        470        480        490        500
LPSNQLSKFP TQISLAQLRL QHIQQQHPPP RLINFQNHSP KPNGPVLPPY 
       510        520        530        540        550
PQQLRYSPSQ NVPRQTTIKP NPLQMAFLAQ QAIKQWQISS VQAPPTTASS 
       560        570        580        590        600
SSSTPSSPTI TSAAGYDGKA FSSPMIDLSA PVGGSYNLPS LPDIDCSSTI 
       610        620        630        640        650
MLDNIARKDT GVDHAQPRPP SNRTVQSPNS SVPSPGLAGP VTMTSVHPPI 
       660        670        680        690        700
RSPSASSVGS RGSSGSSSKP AGADSTHKVP VVMLEPIRIK QENSGPPENY 
       710        720        730        740        750
DFPVVIVKQE SDEESRPQNT NYPRSILTSL LLNSSQSSAS EETVLRSDAP 
       760        770        780        790        800
DSTGDQPGLH QENSSNGKSE WSDASQKSPV HVGETRKEDD PNEDWCAVCQ 
       810        820        830        840        850
NGGELLCCEK CPKVFHLTCH VPTLTNFPSG EWICTFCRDL SKPEVDYDCD 
       860        870        880        890        900
VPSHHSEKRK SEGLTKLTPI DKRKCERLLL FLYCHEMSLA FQDPVPLTVP 
       910        920        930        940        950
DYYKIIKNPM DLSTIKKRLQ EDYCMYTKPE DFVADFRLIF QNCAEFNEPD 
       960        970        980        990       1000
SEVANAGIKL ESYFEELLKN LYPEKRFPKV EFRHEAEDCK FSDDSDDDFV 
      1010 
QPRKKRLKST EDRQLLK

Show »

Length:1,017

Mass (Da):112,844

Checksum:ⁱ0EDAF4BC938F56B6

Computationally mapped potential isoform sequencesⁱ

There is 1 potential isoform mapped to this entry.BLAST Align Show all Add to basket

	Entry	Entry name		Protein names	Gene names	Length	Annotation

	E9Q1U8	E9Q1U8_MOUSE		Transcription intermediary factor 1... Transcription intermediary factor 1-alpha	Trim24	981	Annotation score:

Alternative sequence

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Alternative sequenceⁱVSP_005773	477 – 510	Missing in isoform Short. CuratedAdd BLAST		34

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	S78221 mRNA Translation: AAB34290.1 S78219 mRNA Translation: AAB34289.1 BC056959 mRNA Translation: AAH56959.1
The Consensus CDS (CCDS) project More...CCDSⁱ	CCDS20008.1 [Q64127-1] CCDS71751.1 [Q64127-2]
PIRⁱ	S55259
NCBI Reference Sequences More...RefSeqⁱ	NP_001258993.1, NM_001272064.1 [Q64127-2] NP_001259005.1, NM_001272076.1 NP_659542.3, NM_145076.4 [Q64127-1]
UniGeneⁱ	Mm.41063

Genome annotation databases

Ensemblⁱ	ENSMUST00000031859; ENSMUSP00000031859; ENSMUSG00000029833 [Q64127-1] ENSMUST00000120428; ENSMUSP00000113063; ENSMUSG00000029833 [Q64127-2]
GeneIDⁱ	21848
KEGGⁱ	mmu:21848
UCSC genome browser More...UCSCⁱ	uc009bjk.2 mouse [Q64127-1] uc009bjl.2 mouse [Q64127-2]

Keywords - Coding sequence diversityⁱ

Alternative splicing, Chromosomal rearrangement

Similar proteinsⁱ

90% Identity
50% Identity

Protein	Similar proteins		Species	Score	Length	Source
Q64127	Transcription intermediary factor 1-alpha	)	HUMAN		1050	UniRef90_Q64127
Tripartite motif containing 24		FELCA		1268
Tripartite motif containing 24		PANTR		1054
Transcription intermediary factor 1-alpha isoform b (Fragment)		MACMU		82
Tripartite motif containing 24		BOVIN		1053
+76
Q64127-2	Uncharacterized protein		MACMU		1021	UniRef90_Q64127-2
transcription intermediary factor 1-alpha isoform X2		PHYCD		1018
TRIM24 isoform 2		PONAB		1016
Uncharacterized protein		NOMLE		1016
Tripartite motif containing 24		GORGO		1016
+49

Protein	Similar proteins		Species	Score	Length	Source
Q64127	Transcription intermediary factor 1-alpha	)	HUMAN		1050	UniRef50_Q64127
Tripartite motif containing 24		FELCA		1268
Tripartite motif containing 24		PANTR		1054
Transcription intermediary factor 1-alpha isoform b (Fragment)		MACMU		82
Tripartite motif containing 24		BOVIN		1053
+138

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	S78221 mRNA Translation: AAB34290.1 S78219 mRNA Translation: AAB34289.1 BC056959 mRNA Translation: AAH56959.1
CCDSⁱ	CCDS20008.1 [Q64127-1] CCDS71751.1 [Q64127-2]
PIRⁱ	S55259
RefSeqⁱ	NP_001258993.1, NM_001272064.1 [Q64127-2] NP_001259005.1, NM_001272076.1 NP_659542.3, NM_145076.4 [Q64127-1]
UniGeneⁱ	Mm.41063

3D structure databases

ProteinModelPortalⁱ	Q64127
SMRⁱ	Q64127
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

BioGridⁱ	204196, 11 interactors
DIPⁱ	DIP-31476N
IntActⁱ	Q64127, 13 interactors
MINTⁱ	Q64127
STRINGⁱ	10090.ENSMUSP00000031859

PTM databases

iPTMnetⁱ	Q64127
PhosphoSitePlusⁱ	Q64127

Proteomic databases

PaxDbⁱ	Q64127
PeptideAtlasⁱ	Q64127
PRIDEⁱ	Q64127

Protocols and materials databases

Structural Biology Knowledgebase	Search...

Structural Biology Knowledgebase

Search...

Genome annotation databases

Ensemblⁱ	ENSMUST00000031859; ENSMUSP00000031859; ENSMUSG00000029833 [Q64127-1] ENSMUST00000120428; ENSMUSP00000113063; ENSMUSG00000029833 [Q64127-2]
GeneIDⁱ	21848
KEGGⁱ	mmu:21848
UCSCⁱ	uc009bjk.2 mouse [Q64127-1] uc009bjl.2 mouse [Q64127-2]

Organism-specific databases

CTDⁱ	8805
MGIⁱ	MGI:109275 Trim24

Phylogenomic databases

eggNOGⁱ	ENOG410ITFE Eukaryota COG5076 LUCA
GeneTreeⁱ	ENSGT00920000148978
HOGENOMⁱ	HOG000252971
HOVERGENⁱ	HBG054599
InParanoidⁱ	Q64127
KOⁱ	K08881
OMAⁱ	FWAQNIF
OrthoDBⁱ	EOG091G01KK
PhylomeDBⁱ	Q64127
TreeFamⁱ	TF106455

Enzyme and pathway databases

UniPathwayⁱ	UPA00143

UniPathwayⁱ

UPA00143

Miscellaneous databases

ChiTaRSⁱ	Trim24 mouse
Protein Ontology More...PROⁱ	PR:Q64127
SOURCEⁱ	Search...

Gene expression databases

Bgeeⁱ	ENSMUSG00000029833 Expressed in 308 organ(s), highest expression level in testis
CleanExⁱ	MM_TRIM24
ExpressionAtlasⁱ	Q64127 baseline and differential
Genevisibleⁱ	Q64127 MM

Family and domain databases

CDDⁱ	cd00021 BBOX, 1 hit
Gene3Dⁱ	1.20.920.10, 1 hit
InterProⁱ	View protein in InterPro IPR003649 Bbox_C IPR001487 Bromodomain IPR036427 Bromodomain-like_sf IPR018359 Bromodomain_CS IPR019786 Zinc_finger_PHD-type_CS IPR000315 Znf_B-box IPR011011 Znf_FYVE_PHD IPR001965 Znf_PHD IPR019787 Znf_PHD-finger IPR001841 Znf_RING IPR017907 Znf_RING_CS
Pfamⁱ	View protein in Pfam PF00439 Bromodomain, 1 hit PF00628 PHD, 1 hit PF00643 zf-B_box, 1 hit
PRINTSⁱ	PR00503 BROMODOMAIN
SMARTⁱ	View protein in SMART SM00502 BBC, 1 hit SM00336 BBOX, 2 hits SM00297 BROMO, 1 hit SM00249 PHD, 1 hit SM00184 RING, 1 hit
SUPFAMⁱ	SSF47370 SSF47370, 1 hit SSF57903 SSF57903, 1 hit
PROSITEⁱ	View protein in PROSITE PS00633 BROMODOMAIN_1, 1 hit PS50014 BROMODOMAIN_2, 1 hit PS50119 ZF_BBOX, 2 hits PS01359 ZF_PHD_1, 1 hit PS50016 ZF_PHD_2, 1 hit PS00518 ZF_RING_1, 1 hit PS50089 ZF_RING_2, 1 hit
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	TIF1A_MOUSE
Accessionⁱ	Q64127Primary (citable) accession number: Q64127 Secondary accession number(s): Q64126
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	July 15, 1999
	Last sequence update:	November 1, 1996
	Last modified:	November 7, 2018
	This is version 177 of the entry and version 1 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Chordata Protein Annotation Program

Miscellaneousⁱ

Keywords - Technical termⁱ

Complete proteome, Reference proteome

Documents

MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
PATHWAY comments
Index of metabolic and biosynthesis pathways

UniProtKB

UniParc

Help

UniRef

Proteomes

Annotation systems

Supporting data

UniProtKB - Q64127 (TIF1A_MOUSE)

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Transcription intermediary factor 1-alpha

Trim24

Select a section on the left to see content.

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

<p>This subsection of the <a href="http://www.uniprot.org/help/function_section">'Function'</a> section describes the metabolic pathway(s) associated with a protein.<p><a href='/help/pathway' target='_top'>More...</a></p>Pathwayi: protein ubiquitination

Regions

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

Enzyme and pathway databases

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

Organism-specific databases

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Nucleus

Other locations

Nucleus

Other locations

Keywords - Cellular componenti

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

Mutagenesis

Sites

Keywords - Diseasei

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Amino acid modifications

Keywords - PTMi

Proteomic databases

PTM databases

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

Gene expression databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

Sites

GO - Molecular functioni

Protein-protein interaction databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

Region

Coiled coil

Motif

Compositional bias

Zinc finger

Keywords - Domaini

Phylogenomic databases

Family and domain databases

Alternative sequence

Sequence databases

Genome annotation databases

Keywords - Coding sequence diversityi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Miscellaneous databases

Gene expression databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

Documents

UniProt is an ELIXIR core data resource

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Functionⁱ

Pathwayⁱ: protein ubiquitination

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

Keywords - Cellular componentⁱ

Pathology & Biotechⁱ

Keywords - Diseaseⁱ

PTM / Processingⁱ

Keywords - PTMⁱ

Expressionⁱ

Interactionⁱ

GO - Molecular functionⁱ

Structureⁱ

Family & Domainsⁱ

Keywords - Domainⁱ

Keywords - Coding sequence diversityⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ

Keywords - Technical termⁱ